Restriction map for
pFN21AA1405
(Circular) (Six-base) MAPSORT of: pFN21AA1405.seq Check: 9514 from: 1 to: 7792
pFN21AA1405 7792 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3133 3154 3388 3777 4213 4582 3133
Size: 21 234 389 436 369 6343
Fragments arranged by size:
6343 436 389 369 234 21
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3345 3924 5602 278
Size: 53 83 186 936 1809 579 1678 2468
Fragments arranged by size:
2468 1809 1678 936 579 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4404 5135 1937
Size: 2467 731 4594
Fragments arranged by size:
4594 2467 731
Acc65I G'GTAC_C
Cuts at: 5113 5113
Size: 7792
AclI AA'CG_TT
Cuts at: 1563 5919 6292 1563
Size: 4356 373 3063
Fragments arranged by size:
4356 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 4313 5855 7239 7507 1632
Size: 875 1806 1542 1384 268 1917
Fragments arranged by size:
1917 1806 1542 1384 875 268
AfeI AGC'GCT
Cuts at: 3454 4319 3454
Size: 865 6927
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7595
AflIII A'CryG_T
Cuts at: 1203 2364 6691 7668 1203
Size: 1161 4327 977 1327
Fragments arranged by size:
4327 1327 1161 977
AgeI A'CCGG_T
Cuts at: 6623 6623
Size: 7792
AhdI GACnn_n'nnGTC
Cuts at: 6521 6521
Size: 7792
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7792
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3963 1184
Size: 2779 5013
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2575 2606 2607 2638 2575
Size: 31 1 31 7729
Fragments arranged by size:
7729 31 31 1
AlwNI CAG_nnn'CTG
Cuts at: 2314 2479 3158 3389 4076 4228 4585 4603 4617
Size: 165 679 231 687 152 357 18 14
Cuts at: 4617 5535 7107 2314
Size: 918 1572 2999
Fragments arranged by size:
2999 1572 918 687 679 357 231 165
152 18 14
ApaLI G'TGCA_C
Cuts at: 2540 5849 7005 2540
Size: 3309 1156 3327
Fragments arranged by size:
3327 3309 1156
ApoI r'AATT_y
Cuts at: 1493 5101 5327 5479 7588 1493
Size: 3608 226 152 2109 1697
Fragments arranged by size:
3608 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6346 160
Size: 872 5314 1606
Fragments arranged by size:
5314 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7792
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 3294 3746 5018 5117 1870
Size: 73 185 1166 452 1272 99 4545
Fragments arranged by size:
4545 1272 1166 452 185 99 73
BamHI G'GATC_C
Cuts at: 2320 5122 5489 2320
Size: 2802 367 4623
Fragments arranged by size:
4623 2802 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2132 2171 2258 3145 3765
Size: 325 217 627 345 39 87 887 620
Cuts at: 3765 3814 5113 6568 618
Size: 49 1299 1455 1842
Fragments arranged by size:
1842 1455 1299 887 627 620 345 325
217 87 49 39
BanII G_rGCy'C
Cuts at: 1391 3113 3617 3681 3972 4131 5111 1391
Size: 1722 504 64 291 159 980 4072
Fragments arranged by size:
4072 1722 980 504 291 159 64
BbeI G_GCGC'C
Cuts at: 2136 2175 2136
Size: 39 7753
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3318 3496 928
Size: 971 1419 178 5224
Fragments arranged by size:
5224 1419 971 178
BbvCI CC'TCA_GC
Cuts at: 3420 4889 4940 3420
Size: 1469 51 6272
Fragments arranged by size:
6272 1469 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2648 2682 3275 3309 3943 3977 5983
Size: 34 711 34 593 34 634 34 2006
Cuts at: 5983 6017 1903
Size: 34 3678
Fragments arranged by size:
3678 2006 711 634 593 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5684 6894 5684
Size: 1210 6582
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7792
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2283 2996
Size: 107 122 71 631 605 343 268 713
Cuts at: 2996 3872 3998 4106 4115 6403 136
Size: 876 126 108 9 2288 1525
Fragments arranged by size:
2288 1525 876 713 631 605 343 268
126 122 108 107 71 9
BglII A'GATC_T
Cuts at: 2202 2427 4030 4495 7787 2202
Size: 225 1603 465 3292 2207
Fragments arranged by size:
3292 2207 1603 465 225
BlpI GC'TnA_GC
Cuts at: 4119 5209 4119
Size: 1090 6702
Bme1580I G_kGCm'C
Cuts at: 1790 2544 3150 3768 3819 5853 7009 1790
Size: 754 606 618 51 2034 1156 2573
Fragments arranged by size:
2573 2034 1156 754 618 606 51
BmgBI CAC'GTC
Cuts at: 1318 2191 2857 1318
Size: 873 666 6253
Fragments arranged by size:
6253 873 666
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2104 2548 2822
Size: 416 53 83 399 24 687 444 274
Cuts at: 2822 2912 3522 3966 4396 4715 4746 5523 6481
Size: 90 610 444 430 319 31 777 958
Cuts at: 6481 442
Size: 1753
Fragments arranged by size:
1753 958 777 687 610 444 444 430
416 399 319 274 90 83 53 31
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7792
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3646 3697 4579 3646
Size: 51 882 6859
Fragments arranged by size:
6859 882 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2444 2501 3326 3380 3544 3601 3986 4469
Size: 1205 57 825 54 164 57 385 483
Cuts at: 4469 4538 4607 4655 6452 1239
Size: 69 69 48 1797 2579
Fragments arranged by size:
2579 1797 1205 825 483 385 164 69
69 57 57 54 48
Bpu10I CC'TnA_GC
Cuts at: 3420 4095 4125 4889 4940 3420
Size: 675 30 764 51 6272
Fragments arranged by size:
6272 764 675 51 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2759 3068 5917 6782 7080 2759
Size: 309 2849 865 298 3471
Fragments arranged by size:
3471 2849 865 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3700 6455 882
Size: 617 2201 2755 2219
Fragments arranged by size:
2755 2219 2201 617
BsaAI yAC'GTr
Cuts at: 493 1204 2182 2365 2515 5583 493
Size: 711 978 183 150 3068 2702
Fragments arranged by size:
3068 2702 978 711 183 150
BsaBI GATnn'nnATC
Cuts at: 7786 7786
Size: 7792
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2172 2238 3342
Size: 53 83 186 936 600 39 66 1104
Cuts at: 3342 3921 4899 5599 5981 6616 275
Size: 579 978 700 382 635 1451
Fragments arranged by size:
1451 1104 978 936 700 635 600 579
382 186 83 66 53 39
BsaWI w'CCGG_w
Cuts at: 6225 6623 6897 7044 7455 6225
Size: 398 274 147 411 6562
Fragments arranged by size:
6562 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3164 3194 3524 3554 4822
Size: 30 165 30 1761 30 330 30 1268
Cuts at: 4822 4852 1178
Size: 30 4118
Fragments arranged by size:
4118 1761 1268 330 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3083 3185 3251 3377 3689 3725 3815 3874
Size: 1897 102 66 126 312 36 90 59
Cuts at: 3874 4814 1186
Size: 940 4164
Fragments arranged by size:
4164 1897 940 312 126 102 90 66
59 36
BseYI C'CCAG_C
Cuts at: 3113 3761 3772 4659 4998 6995 7491 3113
Size: 648 11 887 339 1997 496 3414
Fragments arranged by size:
3414 1997 887 648 496 339 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2406 3182 3959 4616 1163
Size: 573 670 776 777 657 4339
Fragments arranged by size:
4339 777 776 670 657 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2598 5242 6003 7031 1540
Size: 275 186 597 2644 761 1028 2301
Fragments arranged by size:
2644 2301 1028 761 597 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 2496 2544 2565 5853 5938 7009 2364
Size: 132 48 21 3288 85 1071 3147
Fragments arranged by size:
3288 3147 1071 132 85 48 21
BsmI GAATG_Cn'
Cuts at: 5303 5396 5303
Size: 93 7699
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2496 2544 2565 3113 3150 3617
Size: 399 574 132 48 21 548 37 467
Cuts at: 3617 3681 3768 3819 3972 4131 5111 5853 5938
Size: 64 87 51 153 159 980 742 85
Cuts at: 5938 7009 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 980 742 574 548 467 399
159 153 132 87 85 64 51 48
37 21
BspEI T'CCGG_A
Cuts at: 7455 7455
Size: 7792
BspHI T'CATG_A
Cuts at: 5681 7411 5681
Size: 1730 6062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3133 3154 3378 3388 3777 3931 3969 4213
Size: 2289 21 224 10 389 154 38 244
Cuts at: 4213 4246 4582 5147 844
Size: 33 336 565 3489
Fragments arranged by size:
3489 2289 565 389 336 244 224 154
38 33 21 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2680 2740 2770 5679 1394
Size: 429 857 60 30 2909 3507
Fragments arranged by size:
3507 2909 857 429 60 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 2824 4845 6287 6461 65
Size: 1183 1576 2021 1442 174 1396
Fragments arranged by size:
2021 1576 1442 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4135 4548 6436 6623 1700
Size: 110 2325 413 1888 187 2869
Fragments arranged by size:
2869 2325 1888 413 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7792
BssHII G'CGCG_C
Cuts at: 1925 2100 2102 2104 1925
Size: 175 2 2 7613
Fragments arranged by size:
7613 175 2 2
BssSI C'ACGA_G
Cuts at: 5852 6864 5852
Size: 1012 6780
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 2539 3814 4225 794
Size: 1520 225 1275 411 4361
Fragments arranged by size:
4361 1520 1275 411 225
BstBI TT'CG_AA
Cuts at: 6596 6596
Size: 7792
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7792
BstYI r'GATC_y
Cuts at: 1972 2202 2320 2427 4030 4495 4686 4884 5122
Size: 230 118 107 1603 465 191 198 238
Cuts at: 5122 5489 5874 5891 7343 7429 7441 7787 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1603 1452 465 385 367 346 238
230 198 191 118 107 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3672 4023 3672
Size: 351 7441
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 2670 2760 3891 513
Size: 552 939 78 588 90 1131 4414
Fragments arranged by size:
4414 1131 939 588 552 90 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7053
Fragments arranged by size:
7053 683 56
BtsI GCAGTG_nn'
Cuts at: 3318 4188 5086 5308 6102 6122 3318
Size: 870 898 222 794 20 4988
Fragments arranged by size:
4988 898 870 794 222 20
ClaI AT'CG_AT
Cuts at: 1554 4527 1554
Size: 2973 4819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7757
DraI TTT'AAA
Cuts at: 5096 5096
Size: 7792
DraIII CAC_nnn'GTG
Cuts at: 2539 2548 2539
Size: 9 7783
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6799 809
Size: 507 5483 1802
Fragments arranged by size:
5483 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 3873 3894
Size: 54 1425 50 275 18 445 1598 21
Cuts at: 3894 4137 4536 5239 6128 7757 8
Size: 243 399 703 889 1629 43
Fragments arranged by size:
1629 1598 1425 889 703 445 399 275
243 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 5239 1537
Size: 275 3427 4090
Fragments arranged by size:
4090 3427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3240 4148 4901 5722 1353
Size: 1887 908 753 821 3423
Fragments arranged by size:
3423 1887 908 821 753
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2313 4535 5003 6379 6753 6899
Size: 1064 138 724 2222 468 1376 374 146
Cuts at: 6899 387
Size: 1280
Fragments arranged by size:
2222 1376 1280 1064 724 468 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 2501 2507 3326 3380 3544 3601
Size: 393 812 57 6 819 54 164 57
Cuts at: 3601 3986 4313 4469 4538 4607 4655 5855 6452
Size: 385 327 156 69 69 48 1200 597
Cuts at: 6452 7239 7507 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1200 819 812 787 597 393 385
327 268 164 156 69 69 57 57
54 48 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3374 3413 3749 4589 3374
Size: 39 336 840 6577
Fragments arranged by size:
6577 840 336 39
EcoO109I rG'GnC_Cy
Cuts at: 2311 2352 2449 3034 3844 3931 3943 4099 4288
Size: 41 97 585 810 87 12 156 189
Cuts at: 4288 4984 2311
Size: 696 5119
Fragments arranged by size:
5119 810 696 585 189 156 97 87
41 12
EcoRI G'AATT_C
Cuts at: 5101 5479 5101
Size: 378 7414
FspI TGC'GCA
Cuts at: 3329 6298 3329
Size: 2969 4823
HaeII r_GCGC'y
Cuts at: 1678 2136 2175 2834 2848 3253 3456 4321 6939
Size: 458 39 659 14 405 203 865 2618
Cuts at: 6939 1678
Size: 2531
Fragments arranged by size:
2618 2531 865 659 458 405 203 39
14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2206 2238 2312 2328 2344
Size: 32 135 32 634 32 74 16 16
Cuts at: 2344 2360 2729 2761 3275 3307 3413 3445 3505
Size: 16 369 32 514 32 106 32 60
Cuts at: 3505 3524 3537 3556 4022 4054 4480 4487 4512
Size: 19 13 19 466 32 426 7 25
Cuts at: 4512 4519 4678 4710 6433 6465 6507 6539 1373
Size: 7 159 32 1723 32 42 32 2626
Fragments arranged by size:
2626 1723 634 514 466 426 369 159
135 106 74 60 42 32 32 32
32 32 32 32 32 32 32 25
19 19 16 16 16 13 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 5136 5382 669
Size: 988 281 3198 246 3079
Fragments arranged by size:
3198 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4466 748
Size: 3718 4074
HpaI GTT'AAC
Cuts at: 5382 5382
Size: 7792
KasI G'GCGC_C
Cuts at: 2132 2171 2132
Size: 39 7753
KpnI G_GTAC'C
Cuts at: 5117 5117
Size: 7792
MfeI C'AATT_G
Cuts at: 5391 5391
Size: 7792
MluI A'CGCG_T
Cuts at: 7668 7668
Size: 7792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3708 4068 4227 4808 6906 7090 1305
Size: 2403 360 159 581 2098 184 2007
Fragments arranged by size:
2403 2098 2007 581 360 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3875 3896 4538 10
Size: 54 1425 343 2043 21 642 3264
Fragments arranged by size:
3264 2043 1425 642 343 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 2660 3323 4175 5750 6109 6268
Size: 648 1443 51 663 852 1575 359 159
Cuts at: 6268 518
Size: 2042
Fragments arranged by size:
2042 1575 1443 852 663 648 359 159
51
MspA1I CmG'CkG
Cuts at: 1606 2056 2227 2289 3226 3761 4145 4201 4243
Size: 450 171 62 937 535 384 56 42
Cuts at: 4243 4345 4380 4516 4944 5208 5885 7033 7278
Size: 102 35 136 428 264 677 1148 245
Cuts at: 7278 1606
Size: 2120
Fragments arranged by size:
2120 1148 937 677 535 450 428 384
264 245 171 136 102 62 56 42
35
NaeI GCC'GGC
Cuts at: 4137 4550 4137
Size: 413 7379
NarI GG'CG_CC
Cuts at: 2133 2172 2133
Size: 39 7753
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6301
Fragments arranged by size:
6301 939 552
NdeI CA'TA_TG
Cuts at: 387 3337 387
Size: 2950 4842
NgoMIV G'CCGG_C
Cuts at: 4135 4548 4135
Size: 413 7379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7792
NotI GC'GGCC_GC
Cuts at: 5239 5239
Size: 7792
NruI TCG'CGA
Cuts at: 3057 3057
Size: 7792
NspI r_CATG'y
Cuts at: 1132 1229 5150 6695 1132
Size: 97 3921 1545 2229
Fragments arranged by size:
3921 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2349 3665 1348
Size: 1001 1316 5475
Fragments arranged by size:
5475 1316 1001
PciI A'CATG_T
Cuts at: 6691 6691
Size: 7792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2083 1108
Size: 975 6817
PfoI T'CCnGG_A
Cuts at: 2445 3231 3716 3732 4416 4584 2445
Size: 786 485 16 684 168 5653
Fragments arranged by size:
5653 786 684 485 168 16
PmeI GTTT'AAAC
Cuts at: 5096 5096
Size: 7792
PmlI CAC'GTG
Cuts at: 2182 2365 2182
Size: 183 7609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2351 2383 5861 5893 7374 7406 2351
Size: 32 3478 32 1481 32 2737
Fragments arranged by size:
3478 2737 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 3034 3844 3931 3943 4288 2449
Size: 585 810 87 12 345 5953
Fragments arranged by size:
5953 810 585 345 87 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7792
PsiI TTA'TAA
Cuts at: 5362 5362
Size: 7792
PspXI vC'TCGA_Gb
Cuts at: 5018 5018
Size: 7792
PstI C_TGCA'G
Cuts at: 830 2895 3399 3825 3843 4242 4362 4503 4774
Size: 2065 504 426 18 399 120 141 271
Cuts at: 4774 5144 830
Size: 370 3478
Fragments arranged by size:
3478 2065 504 426 399 370 271 141
120 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7792
PvuII CAG'CTG
Cuts at: 3226 4145 4243 4345 4516 4944 3226
Size: 919 98 102 171 428 6074
Fragments arranged by size:
6074 919 428 171 102 98
SalI G'TCGA_C
Cuts at: 1936 5134 1936
Size: 3198 4594
SapI GCTCTTCn'nnn_
Cuts at: 3240 4901 3240
Size: 1661 6131
SbfI CC_TGCA'GG
Cuts at: 3399 3825 3843 5144 3399
Size: 426 18 1301 6047
Fragments arranged by size:
6047 1301 426 18
ScaI AGT'ACT
Cuts at: 6040 7752 6040
Size: 1712 6080
SexAI A'CCwGG_T
Cuts at: 2715 2715
Size: 7792
SfcI C'TryA_G
Cuts at: 826 1045 2891 3395 3821 3839 4238 4358 4499
Size: 219 1846 504 426 18 399 120 141
Cuts at: 4499 4624 4770 4810 5140 6275 6956 7147 826
Size: 125 146 40 330 1135 681 191 1471
Fragments arranged by size:
1846 1471 1135 681 504 426 399 330
219 191 146 141 125 120 40 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 4106 4106
Size: 7792
SfoI GGC'GCC
Cuts at: 2134 2173 2134
Size: 39 7753
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7792
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7792
SmaI CCC'GGG
Cuts at: 1872 2130 5119 1872
Size: 258 2989 4545
Fragments arranged by size:
4545 2989 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2774 3083 5018 5896 6797 7059
Size: 197 926 831 309 1935 878 901 262
Cuts at: 7059 820
Size: 1553
Fragments arranged by size:
1935 1553 926 901 878 831 309 262
197
SnaBI TAC'GTA
Cuts at: 493 5583 493
Size: 5090 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7792
SphI G_CATG'C
Cuts at: 1132 5150 1132
Size: 4018 3774
SspI AAT'ATT
Cuts at: 5 52 5716 5
Size: 47 5664 2081
Fragments arranged by size:
5664 2081 47
StuI AGG'CCT
Cuts at: 1517 3837 4021 4938 1517
Size: 2320 184 917 4371
Fragments arranged by size:
4371 2320 917 184
StyI C'CwwG_G
Cuts at: 513 1065 2004 2455 3039 3498 3579 3927 5043
Size: 552 939 451 584 459 81 348 1116
Cuts at: 5043 5231 513
Size: 188 3074
Fragments arranged by size:
3074 1116 939 584 552 459 451 348
188 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2511 2584 4253 4378 4990 4994 5819
Size: 1255 931 73 1669 125 612 4 825
Cuts at: 5819 5836 5989 6174 6619 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1669 1498 1255 931 825 612 445 185
153 125 73 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6038 7750 96
Size: 274 80 33 51 1443 4061 1712 138
Fragments arranged by size:
4061 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4822 4854 7752 7784 4822
Size: 32 2898 32 4830
Fragments arranged by size:
4830 2898 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2189 3536 1316
Size: 873 1347 5572
Fragments arranged by size:
5572 1347 873
XbaI T'CTAG_A
Cuts at: 5128 5128
Size: 7792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2923 2476
Size: 447 7345
XhoI C'TCGA_G
Cuts at: 1943 5018 1943
Size: 3075 4717
XmaI C'CCGG_G
Cuts at: 1870 2128 5117 1870
Size: 258 2989 4545
Fragments arranged by size:
4545 2989 258
XmnI GAAnn'nnTTC
Cuts at: 2297 5921 2297
Size: 3624 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3343 3922 5600 276
Size: 53 83 186 936 1809 579 1678 2468
Fragments arranged by size:
2468 1809 1678 936 579 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NsiI PacI
PspOMI PsrI RsrII SacI SacII SanDI SrfI SwaI