Restriction map for
pFN21AA1404
(Circular) (Six-base) MAPSORT of: pFN21AA1404.seq Check: 5492 from: 1 to: 10459
pFN21AA1404 10459 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8269 278
Size: 53 83 186 936 6733 2468
Fragments arranged by size:
6733 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3285 7802 1937
Size: 1348 4517 4594
Fragments arranged by size:
4594 4517 1348
Acc65I G'GTAC_C
Cuts at: 7780 7780
Size: 10459
AclI AA'CG_TT
Cuts at: 1563 8586 8959 1563
Size: 7023 373 3063
Fragments arranged by size:
7023 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3335 3681 3812 4091 7062 8522 9906 10174
Size: 1703 346 131 279 2971 1460 1384 268
Cuts at: 10174 1632
Size: 1917
Fragments arranged by size:
2971 1917 1703 1460 1384 346 279 268
131
AfeI AGC'GCT
Cuts at: 7238 7238
Size: 10459
AflII C'TTAA_G
Cuts at: 820 1017 5297 6995 820
Size: 197 4280 1698 4284
Fragments arranged by size:
4284 4280 1698 197
AflIII A'CryG_T
Cuts at: 1203 5127 7397 7607 9358 10335 1203
Size: 3924 2270 210 1751 977 1327
Fragments arranged by size:
3924 2270 1751 1327 977 210
AgeI A'CCGG_T
Cuts at: 5997 6241 9290 5997
Size: 244 3049 7166
Fragments arranged by size:
7166 3049 244
AhdI GACnn_n'nnGTC
Cuts at: 9188 9188
Size: 10459
AleI CACnn'nnGTG
Cuts at: 1166 2966 6171 6424 1166
Size: 1800 3205 253 5201
Fragments arranged by size:
5201 3205 1800 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5531 6422 1184
Size: 4347 891 5221
Fragments arranged by size:
5221 4347 891
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 6069 6101 5352
Size: 32 685 32 9710
Fragments arranged by size:
9710 685 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3202 3704 4000 4249 4409 4637 4967 5110 5275
Size: 502 296 249 160 228 330 143 165
Cuts at: 5275 5713 8202 9774 3202
Size: 438 2489 1572 3887
Fragments arranged by size:
3887 2489 1572 502 438 330 296 249
228 165 160 143
ApaLI G'TGCA_C
Cuts at: 3038 4216 6571 8516 9672 3038
Size: 1178 2355 1945 1156 3825
Fragments arranged by size:
3825 2355 1945 1178 1156
ApoI r'AATT_y
Cuts at: 1493 2044 2648 3334 3710 4109 7768 7994 8146
Size: 551 604 686 376 399 3659 226 152
Cuts at: 8146 10255 1493
Size: 2109 1697
Fragments arranged by size:
3659 2109 1697 686 604 551 399 376
226 152
AseI AT'TA_AT
Cuts at: 160 1032 3750 9013 160
Size: 872 2718 5263 1606
Fragments arranged by size:
5263 2718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10459
AvaI C'yCGr_G
Cuts at: 1870 1943 5670 7784 1870
Size: 73 3727 2114 4545
Fragments arranged by size:
4545 3727 2114 73
AvrII C'CTAG_G
Cuts at: 2165 2165
Size: 10459
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4138 4171 4808 4841 4853 4886 5808 5841 4138
Size: 33 637 33 12 33 922 33 8756
Fragments arranged by size:
8756 922 637 33 33 33 33 12
BamHI G'GATC_C
Cuts at: 7789 8156 7789
Size: 367 10092
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4773 5722 5994 6046 6874
Size: 325 217 627 2986 949 272 52 828
Cuts at: 6874 7780 9235 618
Size: 906 1455 1842
Fragments arranged by size:
2986 1842 1455 949 906 828 627 325
272 217 52
BanII G_rGCy'C
Cuts at: 1391 2523 2562 6594 6962 7693 7778 1391
Size: 1132 39 4032 368 731 85 4072
Fragments arranged by size:
4072 4032 1132 731 368 85 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 2450 2767 2991 3198 4031 4920
Size: 971 124 427 317 224 207 833 889
Cuts at: 4920 5316 6777 7050 928
Size: 396 1461 273 4337
Fragments arranged by size:
4337 1461 971 889 833 427 396 317
273 224 207 124
BbvCI CC'TCA_GC
Cuts at: 4094 4853 4094
Size: 759 9700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3080 3114 6591 6625 8650 8684 1903
Size: 34 1143 34 3477 34 2025 34 3678
Fragments arranged by size:
3678 3477 2025 1143 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2733 2947 3106 5836 8351 9561 2733
Size: 214 159 2730 2515 1210 3631
Fragments arranged by size:
3631 2730 2515 1210 214 159
BclI T'GATC_A
Cuts at: 1548 2688 1548
Size: 1140 9319
BfrBI ATG'CAT
Cuts at: 3369 5958 6441 7171 3369
Size: 2589 483 730 6657
Fragments arranged by size:
6657 2589 730 483
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4546 7557 9070
Size: 107 122 71 631 605 2874 3011 1513
Cuts at: 9070 136
Size: 1525
Fragments arranged by size:
3011 2874 1525 1513 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3430 10454 3430
Size: 7024 3435
BlpI GC'TnA_GC
Cuts at: 6961 7876 6961
Size: 915 9544
Bme1580I G_kGCm'C
Cuts at: 1790 2942 3042 4220 5234 5727 5997 6051 6156
Size: 1152 100 1178 1014 493 270 54 105
Cuts at: 6156 6234 6455 6575 6879 7607 8520 9676 1790
Size: 78 221 120 304 728 913 1156 2573
Fragments arranged by size:
2573 1178 1156 1152 1014 913 728 493
304 270 221 120 105 100 78 54
BmgBI CAC'GTC
Cuts at: 1318 3556 1318
Size: 2238 8221
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2444 2636 3714
Size: 416 53 83 399 24 1027 192 1078
Cuts at: 3714 4285 4635 5112 8190 9148 442
Size: 571 350 477 3078 958 1753
Fragments arranged by size:
3078 1753 1078 1027 958 571 477 416
399 350 192 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3488 1056
Size: 2432 8027
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2037 3640 6148 2037
Size: 1603 2508 6348
Fragments arranged by size:
6348 2508 1603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 3554 3929 4232 4373 4383 4892 5297
Size: 1502 813 375 303 141 10 509 405
Cuts at: 5297 7028 9119 1239
Size: 1731 2091 2579
Fragments arranged by size:
2579 2091 1731 1502 813 509 405 375
303 141 10
Bpu10I CC'TnA_GC
Cuts at: 4094 4853 4094
Size: 759 9700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 5060 5177 6206 7261 7409 8584 9449 9747
Size: 2484 117 1029 1055 148 1175 865 298
Cuts at: 9747 2576
Size: 3288
Fragments arranged by size:
3288 2484 1175 1055 1029 865 298 148
117
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2277 6665 9122 882
Size: 617 778 4388 2457 2219
Fragments arranged by size:
4388 2457 2219 778 617
BsaAI yAC'GTr
Cuts at: 493 1204 7608 8250 493
Size: 711 6404 642 2702
Fragments arranged by size:
6404 2702 711 642
BsaBI GATnn'nnATC
Cuts at: 4475 5468 6935 10453 4475
Size: 993 1467 3518 4481
Fragments arranged by size:
4481 3518 1467 993
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5538 8266 8648 9283
Size: 53 83 186 936 4005 2728 382 635
Cuts at: 9283 275
Size: 1451
Fragments arranged by size:
4005 2728 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5997 6241 8892 9290 9564 9711 10122 5997
Size: 244 2651 398 274 147 411 6334
Fragments arranged by size:
6334 2651 411 398 274 244 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 3629 3659 4045
Size: 30 165 30 1329 30 867 30 386
Cuts at: 4045 4075 1178
Size: 30 7562
Fragments arranged by size:
7562 1329 867 386 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2151 2154 2744 4454 4469 4472 4535
Size: 838 127 3 590 1710 15 3 63
Cuts at: 4535 5582 7013 7754 1186
Size: 1047 1431 741 3891
Fragments arranged by size:
3891 1710 1431 1047 838 741 590 127
63 15 3 3
BseYI C'CCAG_C
Cuts at: 4269 4542 4671 7692 9662 10158 4269
Size: 273 129 3021 1970 496 4570
Fragments arranged by size:
4570 3021 1970 496 273 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2813 2990 3314 4322 4904 5893 6209
Size: 573 1077 177 324 1008 582 989 316
Cuts at: 6209 6782 7451 7523 1163
Size: 573 669 72 4099
Fragments arranged by size:
4099 1077 1008 989 669 582 573 573
324 316 177 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7909 8670 9698 1540
Size: 275 186 5908 761 1028 2301
Fragments arranged by size:
5908 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 2562 2733 3042 4220 6575 8520 8605 9676
Size: 39 171 309 1178 2355 1945 85 1071
Cuts at: 9676 2523
Size: 3306
Fragments arranged by size:
3306 2355 1945 1178 1071 309 171 85
39
BsmI GAATG_Cn'
Cuts at: 4339 7970 8063 4339
Size: 3631 93 6735
Fragments arranged by size:
6735 3631 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2562 2733 2942 3042 4220 5234
Size: 399 733 39 171 209 100 1178 1014
Cuts at: 5234 5727 5997 6051 6156 6234 6455 6575 6594
Size: 493 270 54 105 78 221 120 19
Cuts at: 6594 6879 6962 7607 7693 7778 8520 8605 9676
Size: 285 83 645 86 85 742 85 1071
Cuts at: 9676 1391
Size: 2174
Fragments arranged by size:
2174 1178 1071 1014 742 733 645 493
399 285 270 221 209 171 120 105
100 86 85 85 83 78 54 39
19
BspEI T'CCGG_A
Cuts at: 10122 10122
Size: 10459
BspHI T'CATG_A
Cuts at: 4158 4192 4287 8348 10078 4158
Size: 34 95 4061 1730 4539
Fragments arranged by size:
4539 4061 1730 95 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4257 4926 5460 7814 844
Size: 3413 669 534 2354 3489
Fragments arranged by size:
3489 3413 2354 669 534
BsrBI CCG'CTC
Cuts at: 1394 1823 8346 1394
Size: 429 6523 3507
Fragments arranged by size:
6523 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5052 7561 8954 9128 65
Size: 1183 3804 2509 1393 174 1396
Fragments arranged by size:
3804 2509 1396 1393 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5997 6241 9103 9290 1700
Size: 110 4187 244 2862 187 2869
Fragments arranged by size:
4187 2869 2862 244 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10459
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10459
BssSI C'ACGA_G
Cuts at: 7530 8519 9531 7530
Size: 989 1012 8458
Fragments arranged by size:
8458 1012 989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5073 794
Size: 4279 6180
BstBI TT'CG_AA
Cuts at: 6363 9263 6363
Size: 2900 7559
BstEII G'GTnAC_C
Cuts at: 1184 6208 6712 1184
Size: 5024 504 4931
Fragments arranged by size:
5024 4931 504
BstXI CCAn_nnnn'nTGG
Cuts at: 4210 4351 6173 4210
Size: 141 1822 8496
Fragments arranged by size:
8496 1822 141
BstYI r'GATC_y
Cuts at: 1972 3430 3760 4848 4983 5272 6693 6951 7743
Size: 1458 330 1088 135 289 1421 258 792
Cuts at: 7743 7789 8156 8541 8558 10010 10096 10108 10454
Size: 46 367 385 17 1452 86 12 346
Cuts at: 10454 1972
Size: 1977
Fragments arranged by size:
1977 1458 1452 1421 1088 792 385 367
346 330 289 258 135 86 46 17
12
Bsu36I CC'TnA_GG
Cuts at: 2976 3203 4085 6893 2976
Size: 227 882 2808 6542
Fragments arranged by size:
6542 2808 882 227
BtgI C'CryG_G
Cuts at: 513 1065 2004 2220 2357 7593 513
Size: 552 939 216 137 5236 3379
Fragments arranged by size:
5236 3379 939 552 216 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9720
Fragments arranged by size:
9720 683 56
BtsI GCAGTG_nn'
Cuts at: 2387 3096 7975 8769 8789 2387
Size: 709 4879 794 20 4057
Fragments arranged by size:
4879 4057 794 709 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10459
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10424
DraI TTT'AAA
Cuts at: 7763 7763
Size: 10459
DraIII CAC_nnn'GTG
Cuts at: 2316 5413 6306 2316
Size: 3097 893 6469
Fragments arranged by size:
6469 3097 893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9466 809
Size: 507 8150 1802
Fragments arranged by size:
8150 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3766 4599 4893
Size: 54 1425 50 275 18 1936 833 294
Cuts at: 4893 5139 5715 6070 6643 7140 7227 7558 7716
Size: 246 576 355 573 497 87 331 158
Cuts at: 7716 7906 8795 10424 8
Size: 190 889 1629 43
Fragments arranged by size:
1936 1629 1425 889 833 576 573 497
355 331 294 275 246 190 158 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7906 1537
Size: 275 6094 4090
Fragments arranged by size:
6094 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2862 3203 4521 4554 5501 5568 5941
Size: 846 663 341 1318 33 947 67 373
Cuts at: 5941 6921 7010 7176 7488 8389 1353
Size: 980 89 166 312 901 3423
Fragments arranged by size:
3423 1318 980 947 901 846 663 373
341 312 166 89 67 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2401 4103 4564 5876 9046 9420
Size: 1064 138 812 1702 461 1312 3170 374
Cuts at: 9420 9566 387
Size: 146 1280
Fragments arranged by size:
3170 1702 1312 1280 1064 812 461 374
146 138
EcoICRI GAG'CTC
Cuts at: 2521 2560 2521
Size: 39 10420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2741 3335 3554 3681 3812 3929 4091
Size: 393 1109 594 219 127 131 117 162
Cuts at: 4091 4232 4373 4383 4892 5297 7028 7062 8522
Size: 141 141 10 509 405 1731 34 1460
Cuts at: 8522 9119 9906 10174 1239
Size: 597 787 268 1524
Fragments arranged by size:
1731 1524 1460 1109 787 597 594 509
405 393 268 219 162 141 141 131
127 117 34 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2756 2891 3601 3870 4991 5441 6339 2756
Size: 135 710 269 1121 450 898 6876
Fragments arranged by size:
6876 1121 898 710 450 269 135
EcoO109I rG'GnC_Cy
Cuts at: 2066 2761 3054 3199 3860 3907 4143 4406 4539
Size: 695 293 145 661 47 236 263 133
Cuts at: 4539 5774 7000 7096 2066
Size: 1235 1226 96 5429
Fragments arranged by size:
5429 1235 1226 695 661 293 263 236
145 133 96 47
EcoRI G'AATT_C
Cuts at: 2044 3334 4109 7768 8146 2044
Size: 1290 775 3659 378 4357
Fragments arranged by size:
4357 3659 1290 775 378
EcoRV GAT'ATC
Cuts at: 5643 5643
Size: 10459
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2540 3400 7255 7478 2540
Size: 860 3855 223 5521
Fragments arranged by size:
5521 3855 860 223
FspI TGC'GCA
Cuts at: 5507 8965 5507
Size: 3458 7001
HaeII r_GCGC'y
Cuts at: 1678 6406 6556 7240 9606 1678
Size: 4728 150 684 2366 2531
Fragments arranged by size:
4728 2531 2366 684 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3432 3464 6840 6872 7432
Size: 32 135 32 1860 32 3376 32 560
Cuts at: 7432 7464 9100 9132 9174 9206 1373
Size: 32 1636 32 42 32 2626
Fragments arranged by size:
3376 2626 1860 1636 560 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2860 6239 7803 8049 669
Size: 988 281 922 3379 1564 246 3079
Fragments arranged by size:
3379 3079 1564 988 922 281 246
HindIII A'AGCT_T
Cuts at: 748 3184 5070 5832 748
Size: 2436 1886 762 5375
Fragments arranged by size:
5375 2436 1886 762
HpaI GTT'AAC
Cuts at: 8049 8049
Size: 10459
KpnI G_GTAC'C
Cuts at: 7784 7784
Size: 10459
MfeI C'AATT_G
Cuts at: 4015 8058 4015
Size: 4043 6416
MluI A'CGCG_T
Cuts at: 10335 10335
Size: 10459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5352 5670 9573 9757 1305
Size: 4047 318 3903 184 2007
Fragments arranged by size:
4047 3903 2007 318 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3768 4601 5141 5717 6645
Size: 54 1425 343 1936 833 540 576 928
Cuts at: 6645 7142 7229 7560 10
Size: 497 87 331 2909
Fragments arranged by size:
2909 1936 1425 928 833 576 540 497
343 331 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2028 2966 3045 3260 5657 5708 5928
Size: 648 862 938 79 215 2397 51 220
Cuts at: 5928 6171 6424 6705 7566 8417 8776 8935 518
Size: 243 253 281 861 851 359 159 2042
Fragments arranged by size:
2397 2042 938 862 861 851 648 359
281 253 243 220 215 159 79 51
MspA1I CmG'CkG
Cuts at: 1606 3481 4546 4637 5110 6357 6466 6483 6766
Size: 1875 1065 91 473 1247 109 17 283
Cuts at: 6766 6919 7033 7875 8552 9700 9945 1606
Size: 153 114 842 677 1148 245 2120
Fragments arranged by size:
2120 1875 1247 1148 1065 842 677 473
283 245 153 114 109 91 17
NcoI C'CATG_G
Cuts at: 513 1065 2004 2220 2357 7593 513
Size: 552 939 216 137 5236 3379
Fragments arranged by size:
5236 3379 939 552 216 137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10459
NheI G'CTAG_C
Cuts at: 1052 3484 1052
Size: 2432 8027
NotI GC'GGCC_GC
Cuts at: 7906 7906
Size: 10459
NsiI A_TGCA'T
Cuts at: 3371 5960 6443 7173 3371
Size: 2589 483 730 6657
Fragments arranged by size:
6657 2589 730 483
NspI r_CATG'y
Cuts at: 1132 1229 2267 3373 5707 5906 7175 7401 7700
Size: 97 1038 1106 2334 199 1269 226 299
Cuts at: 7700 7817 9362 1132
Size: 117 1545 2229
Fragments arranged by size:
2334 2229 1545 1269 1106 1038 299 226
199 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10459
PciI A'CATG_T
Cuts at: 7397 9358 7397
Size: 1961 8498
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 4000 4582 5275 5554 6443 7149 1108
Size: 1518 1374 582 693 279 889 706 4418
Fragments arranged by size:
4418 1518 1374 889 706 693 582 279
PfoI T'CCnGG_A
Cuts at: 2757 3558 3570 3579 3865 4908 6018 2757
Size: 801 12 9 286 1043 1110 7198
Fragments arranged by size:
7198 1110 1043 801 286 12 9
PmeI GTTT'AAAC
Cuts at: 7763 7763
Size: 10459
PmlI CAC'GTG
Cuts at: 7608 7608
Size: 10459
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8528 8560 10041 10073 8528
Size: 32 1481 32 8914
Fragments arranged by size:
8914 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2761 3860 4406 7096 2761
Size: 1099 546 2690 6124
Fragments arranged by size:
6124 2690 1099 546
PshAI GACnn'nnGTC
Cuts at: 1613 7101 1613
Size: 5488 4971
PsiI TTA'TAA
Cuts at: 8029 8029
Size: 10459
PstI C_TGCA'G
Cuts at: 830 2466 2922 4254 4744 5640 5870 6779 7811
Size: 1636 456 1332 490 896 230 909 1032
Cuts at: 7811 830
Size: 3478
Fragments arranged by size:
3478 1636 1332 1032 909 896 490 456
230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10459
PvuII CAG'CTG
Cuts at: 3481 4637 5110 6357 6766 7033 3481
Size: 1156 473 1247 409 267 6907
Fragments arranged by size:
6907 1247 1156 473 409 267
SacI G_AGCT'C
Cuts at: 2523 2562 2523
Size: 39 10420
SalI G'TCGA_C
Cuts at: 1936 7801 1936
Size: 5865 4594
SapI GCTCTTCn'nnn_
Cuts at: 5501 7010 7488 5501
Size: 1509 478 8472
Fragments arranged by size:
8472 1509 478
SbfI CC_TGCA'GG
Cuts at: 7811 7811
Size: 10459
ScaI AGT'ACT
Cuts at: 5432 8707 10419 5432
Size: 3275 1712 5472
Fragments arranged by size:
5472 3275 1712
SexAI A'CCwGG_T
Cuts at: 2312 7299 2312
Size: 4987 5472
SfcI C'TryA_G
Cuts at: 826 1045 2338 2406 2462 2918 3547 4250 4740
Size: 219 1293 68 56 456 629 703 490
Cuts at: 4740 4817 4988 5636 5866 6775 7369 7807 8942
Size: 77 171 648 230 909 594 438 1135
Cuts at: 8942 9623 9814 826
Size: 681 191 1471
Fragments arranged by size:
1471 1293 1135 909 703 681 648 629
594 490 456 438 230 219 191 171
77 68 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10459
SgrAI Cr'CCGG_yG
Cuts at: 1700 5997 1700
Size: 4297 6162
SmaI CCC'GGG
Cuts at: 1872 7786 1872
Size: 5914 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 5039 5156 5297 6221 6995
Size: 197 926 612 2484 117 141 924 774
Cuts at: 6995 7240 7388 8563 9464 9726 820
Size: 245 148 1175 901 262 1553
Fragments arranged by size:
2484 1553 1175 926 924 901 774 612
262 245 197 148 141 117
SnaBI TAC'GTA
Cuts at: 493 8250 493
Size: 7757 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10459
SphI G_CATG'C
Cuts at: 1132 2267 7700 7817 1132
Size: 1135 5433 117 3774
Fragments arranged by size:
5433 3774 1135 117
SspI AAT'ATT
Cuts at: 5 52 6723 8383 5
Size: 47 6671 1660 2081
Fragments arranged by size:
6671 2081 1660 47
StuI AGG'CCT
Cuts at: 1517 3545 6267 1517
Size: 2028 2722 5709
Fragments arranged by size:
5709 2722 2028
StyI C'CwwG_G
Cuts at: 513 1065 2004 2165 2220 2357 3375 3641 5566
Size: 552 939 161 55 137 1018 266 1925
Cuts at: 5566 5718 7593 7898 513
Size: 152 1875 305 3074
Fragments arranged by size:
3074 1925 1875 1018 939 552 305 266
161 152 137 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4034 6183 6731 7424 8486 8503 8656
Size: 1255 2454 2149 548 693 1062 17 153
Cuts at: 8656 8841 9286 325
Size: 185 445 1498
Fragments arranged by size:
2454 2149 1498 1255 1062 693 548 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2623 4257 5430
Size: 274 80 33 51 1443 646 1634 1173
Cuts at: 5430 5688 6623 8705 10417 96
Size: 258 935 2082 1712 138
Fragments arranged by size:
2082 1712 1634 1443 1173 935 646 274
258 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4045 4077 10419 10451 4045
Size: 32 6342 32 4053
Fragments arranged by size:
6342 4053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10459
XbaI T'CTAG_A
Cuts at: 7795 7795
Size: 10459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4622 6653 6725 4622
Size: 2031 72 8356
Fragments arranged by size:
8356 2031 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10459
XmaI C'CCGG_G
Cuts at: 1870 7784 1870
Size: 5914 4545
XmnI GAAnn'nnTTC
Cuts at: 2457 3591 4076 8588 2457
Size: 1134 485 4512 4328
Fragments arranged by size:
4512 4328 1134 485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8267 276
Size: 53 83 186 936 6733 2468
Fragments arranged by size:
6733 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BbeI BsiWI BsmBI BstZ17I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI