Restriction map for
pFN21AA1402
(Circular) (Six-base) MAPSORT of: pFN21AA1402.seq Check: 5009 from: 1 to: 7021
pFN21AA1402 7021 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2434 4055 2434
Size: 1621 5400
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4831 278
Size: 53 83 186 936 3295 2468
Fragments arranged by size:
3295 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3588 4364 1937
Size: 1651 776 4594
Fragments arranged by size:
4594 1651 776
Acc65I G'GTAC_C
Cuts at: 2936 3669 4342 2936
Size: 733 673 5615
Fragments arranged by size:
5615 733 673
AclI AA'CG_TT
Cuts at: 1563 5148 5521 1563
Size: 3585 373 3063
Fragments arranged by size:
3585 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2950 3519 5084 6468 6736 1632
Size: 1318 569 1565 1384 268 1917
Fragments arranged by size:
1917 1565 1384 1318 569 268
AfeI AGC'GCT
Cuts at: 2043 2971 2043
Size: 928 6093
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6824
AflIII A'CryG_T
Cuts at: 1203 5920 6897 1203
Size: 4717 977 1327
Fragments arranged by size:
4717 1327 977
AgeI A'CCGG_T
Cuts at: 5852 5852
Size: 7021
AhdI GACnn_n'nnGTC
Cuts at: 5750 5750
Size: 7021
AleI CACnn'nnGTG
Cuts at: 1166 2561 3467 1166
Size: 1395 906 4720
Fragments arranged by size:
4720 1395 906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4026 1184
Size: 2842 4179
AlwNI CAG_nnn'CTG
Cuts at: 3289 4764 6336 3289
Size: 1475 1572 3974
Fragments arranged by size:
3974 1572 1475
ApaI G_GGCC'C
Cuts at: 2425 3388 3807 3921 2425
Size: 963 419 114 5525
Fragments arranged by size:
5525 963 419 114
ApaLI G'TGCA_C
Cuts at: 2918 5078 6234 2918
Size: 2160 1156 3705
Fragments arranged by size:
3705 2160 1156
ApoI r'AATT_y
Cuts at: 1493 4330 4556 4708 6817 1493
Size: 2837 226 152 2109 1697
Fragments arranged by size:
2837 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5575 160
Size: 872 4543 1606
Fragments arranged by size:
4543 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2424 3465 3524 3922 3969 4050
Size: 73 388 93 1041 59 398 47 81
Cuts at: 4050 4346 1870
Size: 296 4545
Fragments arranged by size:
4545 1041 398 388 296 93 81 73
59 47
BamHI G'GATC_C
Cuts at: 2103 4351 4718 2103
Size: 2248 367 4406
Fragments arranged by size:
4406 2248 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2936 3375 3669 4342 5797
Size: 325 217 627 1149 439 294 673 1455
Cuts at: 5797 618
Size: 1842
Fragments arranged by size:
1842 1455 1149 673 627 439 325 294
217
BanII G_rGCy'C
Cuts at: 1391 2018 2182 2297 2425 2431 2894 3084 3175
Size: 627 164 115 128 6 463 190 91
Cuts at: 3175 3388 3619 3807 3921 3979 4057 4146 4340
Size: 213 231 188 114 58 78 89 194
Cuts at: 4340 1391
Size: 4072
Fragments arranged by size:
4072 627 463 231 213 194 190 188
164 128 115 114 91 89 78 58
6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2208 928
Size: 971 309 5741
Fragments arranged by size:
5741 971 309
BbvCI CC'TCA_GC
Cuts at: 2600 4244 2600
Size: 1644 5377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2825 2859 3230 3264 3515
Size: 34 50 34 804 34 371 34 251
Cuts at: 3515 3549 5212 5246 1903
Size: 34 1663 34 3678
Fragments arranged by size:
3678 1663 804 371 251 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4913 6123 4913
Size: 1210 5811
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7021
BfrBI ATG'CAT
Cuts at: 3566 3566
Size: 7021
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2476 3079 4061
Size: 107 122 71 631 605 804 603 982
Cuts at: 4061 4280 5632 136
Size: 219 1352 1525
Fragments arranged by size:
1525 1352 982 804 631 605 603 219
122 107 71
BglII A'GATC_T
Cuts at: 7016 7016
Size: 7021
BlpI GC'TnA_GC
Cuts at: 3065 4438 3065
Size: 1373 5648
Bme1580I G_kGCm'C
Cuts at: 1790 2425 2764 2922 3185 3378 3388 3807 3921
Size: 635 339 158 263 193 10 419 114
Cuts at: 3921 4276 5082 6238 1790
Size: 355 806 1156 2573
Fragments arranged by size:
2573 1156 806 635 419 355 339 263
193 158 114 10
BmgBI CAC'GTC
Cuts at: 1318 3211 3739 1318
Size: 1893 528 4600
Fragments arranged by size:
4600 1893 528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2418 4752 5710
Size: 416 53 83 399 24 1001 2334 958
Cuts at: 5710 442
Size: 1753
Fragments arranged by size:
2334 1753 1001 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3201 1056
Size: 2145 4876
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2771 2311
Size: 460 6561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 2996 3257 3569 3603 4121 4130 4274
Size: 1176 581 261 312 34 518 9 144
Cuts at: 4274 5681 1239
Size: 1407 2579
Fragments arranged by size:
2579 1407 1176 581 518 312 261 144
34 9
Bpu10I CC'TnA_GC
Cuts at: 2081 2264 2600 4244 2081
Size: 183 336 1644 4858
Fragments arranged by size:
4858 1644 336 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5146 6011 6309 5146
Size: 865 298 5858
Fragments arranged by size:
5858 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 3748 5684 882
Size: 617 984 1265 1936 2219
Fragments arranged by size:
2219 1936 1265 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 4812 493
Size: 711 3608 2702
Fragments arranged by size:
3608 2702 711
BsaBI GATnn'nnATC
Cuts at: 7015 7015
Size: 7021
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2808 4828 5210 5845
Size: 53 83 186 936 1275 2020 382 635
Cuts at: 5845 275
Size: 1451
Fragments arranged by size:
2020 1451 1275 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3024 4131 5454 5852 6126 6273 6684 3024
Size: 1107 1323 398 274 147 411 3361
Fragments arranged by size:
3361 1323 1107 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2691 2721 2912 2942 3140
Size: 30 165 30 1288 30 191 30 198
Cuts at: 3140 3170 3929 3959 1178
Size: 30 759 30 4240
Fragments arranged by size:
4240 1288 759 198 191 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2166 2651 2760 4103 1186
Size: 980 485 109 1343 4104
Fragments arranged by size:
4104 1343 980 485 109
BseYI C'CCAG_C
Cuts at: 2290 3121 3126 3316 4275 6224 6720 2290
Size: 831 5 190 959 1949 496 2591
Fragments arranged by size:
2591 1949 959 831 496 190 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2095 2585 3183 3491 4199 1163
Size: 573 359 490 598 308 708 3985
Fragments arranged by size:
3985 708 598 573 490 359 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4471 5232 6260 1540
Size: 275 186 2470 761 1028 2301
Fragments arranged by size:
2470 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2018 2182 2265 2894 2922 3619 5082 5167 6238
Size: 164 83 629 28 697 1463 85 1071
Cuts at: 6238 2018
Size: 2801
Fragments arranged by size:
2801 1463 1071 697 629 164 85 83
28
BsiWI C'GTAC_G
Cuts at: 2369 2369
Size: 7021
BsmI GAATG_Cn'
Cuts at: 3838 4532 4625 3838
Size: 694 93 6234
Fragments arranged by size:
6234 694 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2182 2265 2297 2425 2431 2764
Size: 399 228 164 83 32 128 6 333
Cuts at: 2764 2894 2922 3084 3175 3185 3378 3388 3619
Size: 130 28 162 91 10 193 10 231
Cuts at: 3619 3807 3921 3979 4057 4146 4276 4340 5082
Size: 188 114 58 78 89 130 64 742
Cuts at: 5082 5167 6238 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 333 231 228 193
188 164 162 130 130 128 114 91
89 85 83 78 64 58 32 28
10 10 6
BspEI T'CCGG_A
Cuts at: 3024 6684 3024
Size: 3660 3361
BspHI T'CATG_A
Cuts at: 4910 6640 4910
Size: 1730 5291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 3354 4055 4376 844
Size: 1590 920 701 321 3489
Fragments arranged by size:
3489 1590 920 701 321
BsrBI CCG'CTC
Cuts at: 1394 1823 2305 2467 2986 4908 1394
Size: 429 482 162 519 1922 3507
Fragments arranged by size:
3507 1922 519 482 429 162
BsrDI GCAATG_nn'
Cuts at: 65 1248 3566 5516 5690 65
Size: 1183 2318 1950 174 1396
Fragments arranged by size:
2318 1950 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2949 3378 3999 5665 5852 1700
Size: 110 1139 429 621 1666 187 2869
Fragments arranged by size:
2869 1666 1139 621 429 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7021
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7021
BssSI C'ACGA_G
Cuts at: 3555 3597 5081 6093 3555
Size: 42 1484 1012 4483
Fragments arranged by size:
4483 1484 1012 42
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2588 3289 3478 794
Size: 1794 701 189 4337
Fragments arranged by size:
4337 1794 701 189
BstBI TT'CG_AA
Cuts at: 5825 5825
Size: 7021
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7021
BstXI CCAn_nnnn'nTGG
Cuts at: 3547 3547
Size: 7021
BstYI r'GATC_y
Cuts at: 1972 2103 2122 3021 4351 4718 5103 5120 6572
Size: 131 19 899 1330 367 385 17 1452
Cuts at: 6572 6658 6670 7016 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1330 899 385 367 346 131
86 19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2355 3610 4208 513
Size: 552 1290 1255 598 3326
Fragments arranged by size:
3326 1290 1255 598 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6282
Fragments arranged by size:
6282 683 56
BtsI GCAGTG_nn'
Cuts at: 4537 5331 5351 4537
Size: 794 20 6207
Fragments arranged by size:
6207 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7021
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6986
DraI TTT'AAA
Cuts at: 4325 4325
Size: 7021
DraIII CAC_nnn'GTG
Cuts at: 3371 3760 3789 3371
Size: 389 29 6603
Fragments arranged by size:
6603 389 29
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6028 809
Size: 507 4712 1802
Fragments arranged by size:
4712 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 2352 2593
Size: 54 1425 50 275 18 206 316 241
Cuts at: 2593 2723 2859 3413 3607 4468 5357 6986 8
Size: 130 136 554 194 861 889 1629 43
Fragments arranged by size:
1629 1425 889 861 554 316 275 241
206 194 136 130 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4468 1537
Size: 275 2656 4090
Fragments arranged by size:
4090 2656 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3776 4080 4951 1353
Size: 2423 304 871 3423
Fragments arranged by size:
3423 2423 871 304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3221 5608 5982 6128 387
Size: 1064 138 1632 2387 374 146 1280
Fragments arranged by size:
2387 1632 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2016 2180 2892 3617 2016
Size: 164 712 725 5420
Fragments arranged by size:
5420 725 712 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2950 2996 3257 3519 3569 3603
Size: 393 783 535 46 261 262 50 34
Cuts at: 3603 4121 4130 4274 5084 5681 6468 6736 1239
Size: 518 9 144 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 783 597 535 518 393
268 262 261 144 50 46 34 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2230 2230
Size: 7021
EcoO109I rG'GnC_Cy
Cuts at: 2236 2421 2569 2872 3385 3712 3917 3918 2236
Size: 185 148 303 513 327 205 1 5339
Fragments arranged by size:
5339 513 327 303 205 185 148 1
EcoRI G'AATT_C
Cuts at: 4330 4708 4330
Size: 378 6643
FspI TGC'GCA
Cuts at: 5527 5527
Size: 7021
HaeII r_GCGC'y
Cuts at: 1678 2045 2973 3256 3310 6168 1678
Size: 367 928 283 54 2858 2531
Fragments arranged by size:
2858 2531 928 367 283 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2775 2807 3728 3760 3929
Size: 32 135 32 1203 32 921 32 169
Cuts at: 3929 3961 5662 5694 5736 5768 1373
Size: 32 1701 32 42 32 2626
Fragments arranged by size:
2626 1701 1203 921 169 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4365 4611 669
Size: 988 281 2427 246 3079
Fragments arranged by size:
3079 2427 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7021
HpaI GTT'AAC
Cuts at: 4611 4611
Size: 7021
KpnI G_GTAC'C
Cuts at: 2940 3673 4346 2940
Size: 733 673 5615
Fragments arranged by size:
5615 733 673
MfeI C'AATT_G
Cuts at: 4620 4620
Size: 7021
MluI A'CGCG_T
Cuts at: 6897 6897
Size: 7021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2961 3204 3333 6135 6319 1305
Size: 1656 243 129 2802 184 2007
Fragments arranged by size:
2802 2007 1656 243 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2595 2861 10
Size: 54 1425 343 763 266 4170
Fragments arranged by size:
4170 1425 763 343 266 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 2561 2733 3467 3737 4979 5338
Size: 648 1294 101 172 734 270 1242 359
Cuts at: 5338 5497 518
Size: 159 2042
Fragments arranged by size:
2042 1294 1242 734 648 359 270 172
159 101
MspA1I CmG'CkG
Cuts at: 1606 2416 3475 3653 3822 4236 4279 4437 5114
Size: 810 1059 178 169 414 43 158 677
Cuts at: 5114 6262 6507 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1059 810 677 414 245 178
169 158 43
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6469
NdeI CA'TA_TG
Cuts at: 387 2560 387
Size: 2173 4848
NheI G'CTAG_C
Cuts at: 1052 3197 1052
Size: 2145 4876
NotI GC'GGCC_GC
Cuts at: 4468 4468
Size: 7021
NsiI A_TGCA'T
Cuts at: 3568 3568
Size: 7021
NspI r_CATG'y
Cuts at: 1132 1229 3566 4379 5924 1132
Size: 97 2337 813 1545 2229
Fragments arranged by size:
2337 2229 1545 813 97
PasI CC'CwG_GG
Cuts at: 1348 3674 3914 1348
Size: 2326 240 4455
Fragments arranged by size:
4455 2326 240
PciI A'CATG_T
Cuts at: 5920 5920
Size: 7021
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3632 3789 1108
Size: 2524 157 4340
Fragments arranged by size:
4340 2524 157
PfoI T'CCnGG_A
Cuts at: 2099 3546 3873 2099
Size: 1447 327 5247
Fragments arranged by size:
5247 1447 327
PmeI GTTT'AAAC
Cuts at: 4325 4325
Size: 7021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5090 5122 6603 6635 5090
Size: 32 1481 32 5476
Fragments arranged by size:
5476 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2236 2872 2236
Size: 636 6385
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7021
PsiI TTA'TAA
Cuts at: 4591 4591
Size: 7021
PspOMI G'GGCC_C
Cuts at: 2421 3384 3803 3917 2421
Size: 963 419 114 5525
Fragments arranged by size:
5525 963 419 114
PspXI vC'TCGA_Gb
Cuts at: 3524 3524
Size: 7021
PstI C_TGCA'G
Cuts at: 830 3015 3511 3649 4205 4373 830
Size: 2185 496 138 556 168 3478
Fragments arranged by size:
3478 2185 556 496 168 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7021
PvuII CAG'CTG
Cuts at: 3475 4279 3475
Size: 804 6217
SacI G_AGCT'C
Cuts at: 2018 2182 2894 3619 2018
Size: 164 712 725 5420
Fragments arranged by size:
5420 725 712 164
SalI G'TCGA_C
Cuts at: 1936 4363 1936
Size: 2427 4594
SanDI GG'GwC_CC
Cuts at: 2236 2872 2236
Size: 636 6385
SbfI CC_TGCA'GG
Cuts at: 4373 4373
Size: 7021
ScaI AGT'ACT
Cuts at: 5269 6981 5269
Size: 1712 5309
SfcI C'TryA_G
Cuts at: 826 1045 2139 2230 3011 3188 3507 3645 3981
Size: 219 1094 91 781 177 319 138 336
Cuts at: 3981 4201 4369 5504 6185 6376 826
Size: 220 168 1135 681 191 1471
Fragments arranged by size:
1471 1135 1094 781 681 336 319 220
219 191 177 168 138 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7021
SgrAI Cr'CCGG_yG
Cuts at: 1700 3378 1700
Size: 1678 5343
SmaI CCC'GGG
Cuts at: 1872 2426 3924 3971 4348 1872
Size: 554 1498 47 377 4545
Fragments arranged by size:
4545 1498 554 377 47
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3524 5125 6026 6288 820
Size: 197 926 1581 1601 901 262 1553
Fragments arranged by size:
1601 1581 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4812 493
Size: 4319 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7021
SphI G_CATG'C
Cuts at: 1132 4379 1132
Size: 3247 3774
SrfI GCCC'GGGC
Cuts at: 2426 2426
Size: 7021
SspI AAT'ATT
Cuts at: 5 52 4945 5
Size: 47 4893 2081
Fragments arranged by size:
4893 2081 47
StuI AGG'CCT
Cuts at: 1517 3530 3799 3854 1517
Size: 2013 269 55 4684
Fragments arranged by size:
4684 2013 269 55
StyI C'CwwG_G
Cuts at: 513 1065 4460 513
Size: 552 3395 3074
Fragments arranged by size:
3395 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3203 5048 5065 5218 5403 5848 325
Size: 1255 1623 1845 17 153 185 445 1498
Fragments arranged by size:
1845 1623 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 3333 5267
Size: 274 80 33 51 1443 642 714 1934
Cuts at: 5267 6979 96
Size: 1712 138
Fragments arranged by size:
1934 1712 1443 714 642 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2691 2723 2912 2944 3140 3172 6981 7013 2691
Size: 32 189 32 196 32 3809 32 2699
Fragments arranged by size:
3809 2699 196 189 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3740 1316
Size: 2424 4597
XbaI T'CTAG_A
Cuts at: 4357 4357
Size: 7021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2353
Size: 7021
XhoI C'TCGA_G
Cuts at: 1943 3524 1943
Size: 1581 5440
XmaI C'CCGG_G
Cuts at: 1870 2424 3922 3969 4346 1870
Size: 554 1498 47 377 4545
Fragments arranged by size:
4545 1498 554 377 47
XmnI GAAnn'nnTTC
Cuts at: 5150 5150
Size: 7021
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4829 276
Size: 53 83 186 936 3295 2468
Fragments arranged by size:
3295 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsmBI BstZ17I Bsu36I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PsrI RsrII SacII SapI SexAI
SfiI SfoI SwaI