Restriction map for
pFN21AA1376
(Circular) (Six-base) MAPSORT of: pFN21AA1376.seq Check: 970 from: 1 to: 5947
pFN21AA1376 5947 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 2933 3268 2933
Size: 335 5612
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 3054 4010 5394 5662 1632
Size: 621 801 956 1384 268 1917
Fragments arranged by size:
1917 1384 956 801 621 268
AflII C'TTAA_G
Cuts at: 820 1017 2581 820
Size: 197 1564 4186
Fragments arranged by size:
4186 1564 197
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2355 1184
Size: 1171 4776
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 5915
AlwNI CAG_nnn'CTG
Cuts at: 3690 5262 3690
Size: 1572 4375
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 2107 2148 2468 2811 3256 3482 3634 5743
Size: 614 41 320 343 445 226 152 2109
Cuts at: 5743 1493
Size: 1697
Fragments arranged by size:
2109 1697 614 445 343 320 226 152
41
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2671 2933 3268 4723 618
Size: 325 217 627 884 262 335 1455 1842
Fragments arranged by size:
1842 1455 884 627 335 325 262 217
BanII G_rGCy'C
Cuts at: 1391 3127 3266 1391
Size: 1736 139 4072
Fragments arranged by size:
4072 1736 139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2080 3839 5049 2080
Size: 1759 1210 2978
Fragments arranged by size:
2978 1759 1210
BclI T'GATC_A
Cuts at: 1548 3208 1548
Size: 1660 4287
BfrBI ATG'CAT
Cuts at: 2342 2342
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2397 4558 136
Size: 107 122 71 631 605 725 2161 1525
Fragments arranged by size:
2161 1525 725 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2277 4008 5164 1790
Size: 487 1731 1156 2573
Fragments arranged by size:
2573 1731 1156 487
BmgBI CAC'GTC
Cuts at: 1318 2790 1318
Size: 1472 4475
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 3678 4636
Size: 416 53 83 399 24 713 1548 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1548 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2913 4607 1239
Size: 1674 1694 2579
Fragments arranged by size:
2579 1694 1674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2953 4072 4937 5235 2953
Size: 1119 865 298 3665
Fragments arranged by size:
3665 1119 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 3201 5941 3201
Size: 2740 3207
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 1178
Size: 30 165 30 783 30 4909
Fragments arranged by size:
4909 783 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5947
BseYI C'CCAG_C
Cuts at: 2011 3044 5150 5646 2011
Size: 1033 2106 496 2312
Fragments arranged by size:
2312 2106 1033 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4008 4093 5164 4008
Size: 85 1071 4791
Fragments arranged by size:
4791 1071 85
BsmI GAATG_Cn'
Cuts at: 2342 3458 3551 2342
Size: 1116 93 4738
Fragments arranged by size:
4738 1116 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2098 2780 2098
Size: 682 5265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 3127 3266 4008 4093 5164 1391
Size: 399 487 850 139 742 85 1071 2174
Fragments arranged by size:
2174 1071 850 742 487 399 139 85
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2068 4442 4616 65
Size: 1183 820 2374 174 1396
Fragments arranged by size:
2374 1396 1183 820 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 4591 4778 1700
Size: 110 388 2393 187 2869
Fragments arranged by size:
2869 2393 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 3243 4007 5019 3243
Size: 764 1012 4171
Fragments arranged by size:
4171 1012 764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5947
BstBI TT'CG_AA
Cuts at: 2385 4751 2385
Size: 2366 3581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942
Size: 1305 367 385 17 1452 86 12 346
Cuts at: 5942 1972
Size: 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2566 2566
Size: 5947
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4456
Fragments arranged by size:
4456 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 3463 4257 4277 2407
Size: 1056 794 20 4077
Fragments arranged by size:
4077 1056 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2063 4954 809
Size: 507 747 2891 1802
Fragments arranged by size:
2891 1802 747 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912
Size: 54 1425 50 275 18 1564 889 1629
Cuts at: 5912 8
Size: 43
Fragments arranged by size:
1629 1564 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2662 3877 1353
Size: 665 644 1215 3423
Fragments arranged by size:
3423 1215 665 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3094 4534 4908 5054 387
Size: 1064 138 1505 1440 374 146 1280
Fragments arranged by size:
1505 1440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2913 3054 4010 4607 5394 5662
Size: 393 621 660 141 956 597 787 268
Cuts at: 5662 1239
Size: 1524
Fragments arranged by size:
1524 956 787 660 621 597 393 268
141
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
EcoRV GAT'ATC
Cuts at: 2494 2494
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 3215 3247 4588
Size: 32 135 32 506 32 1105 32 1341
Cuts at: 4588 4620 4662 4694 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1341 1105 506 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2038 748
Size: 1290 4657
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 2937 3272 2937
Size: 335 5612
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2337 2400 2996 3905 4264 4423 518
Size: 648 1171 63 596 909 359 159 2042
Fragments arranged by size:
2042 1171 909 648 596 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 3363 4040 5188 5433 1606
Size: 1757 677 1148 245 2120
Fragments arranged by size:
2120 1757 1148 677 245
NaeI GCC'GGC
Cuts at: 2200 2200
Size: 5947
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4456
Fragments arranged by size:
4456 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NgoMIV G'CCGG_C
Cuts at: 2198 2198
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NsiI A_TGCA'T
Cuts at: 2344 2344
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2168 3305 4850 1132
Size: 97 939 1137 1545 2229
Fragments arranged by size:
2229 1545 1137 939 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 2890 3153 2890
Size: 263 5684
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SapI GCTCTTCn'nnn_
Cuts at: 2662 2662
Size: 5947
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2842 3295 4430 5111 5302 826
Size: 219 1797 453 1135 681 191 1471
Fragments arranged by size:
1797 1471 1135 681 453 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2581 2968 4051 4952 5214 820
Size: 197 926 638 387 1083 901 262 1553
Fragments arranged by size:
1553 1083 926 901 638 387 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 2864 3871 5
Size: 47 2812 1007 2081
Fragments arranged by size:
2812 2081 1007 47
StuI AGG'CCT
Cuts at: 1517 2437 2702 1517
Size: 920 265 4762
Fragments arranged by size:
4762 920 265
StyI C'CwwG_G
Cuts at: 513 1065 2004 2438 3386 513
Size: 552 939 434 948 3074
Fragments arranged by size:
3074 948 939 552 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2404 3058 3974 3991 4144 4329 4774
Size: 1255 824 654 916 17 153 185 445
Cuts at: 4774 325
Size: 1498
Fragments arranged by size:
1498 1255 916 824 654 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4193 5905 96
Size: 274 80 33 51 1443 2216 1712 138
Fragments arranged by size:
2216 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 2189 3283 2189
Size: 1094 4853
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 2625 4076 2625
Size: 1451 4496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI
FseI FspAI KasI NarI NruI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI