(Circular) (Six-base) MAPSORT of: pFN21AA1361.seq Check: 1834 from: 1 to: 7708
pFN21AA1361 7708 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2545 4242 2545
Size: 1697 6011
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 5518 278
Size: 53 83 186 936 2287 1695 2468
Fragments arranged by size:
2468 2287 1695 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2201 5051 1937
Size: 264 2850 4594
Fragments arranged by size:
4594 2850 264
Acc65I G'GTAC_C
Cuts at: 4864 5029 4864
Size: 165 7543
AclI AA'CG_TT
Cuts at: 1563 5835 6208 1563
Size: 4272 373 3063
Fragments arranged by size:
4272 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2396 4830 5771 7155 7423 1632
Size: 422 342 2434 941 1384 268 1917
Fragments arranged by size:
2434 1917 1384 941 422 342 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7511
AflIII A'CryG_T
Cuts at: 1203 2645 4995 6607 7584 1203
Size: 1442 2350 1612 977 1327
Fragments arranged by size:
2350 1612 1442 1327 977
AgeI A'CCGG_T
Cuts at: 6539 6539
Size: 7708
AhdI GACnn_n'nnGTC
Cuts at: 6437 6437
Size: 7708
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7708
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3978 4010 3978
Size: 32 7676
AlwNI CAG_nnn'CTG
Cuts at: 4429 4909 4925 5451 7023 4429
Size: 480 16 526 1572 5114
Fragments arranged by size:
5114 1572 526 480 16
ApaLI G'TGCA_C
Cuts at: 5765 6921 5765
Size: 1156 6552
ApoI r'AATT_y
Cuts at: 1493 3608 5017 5243 5395 7504 1493
Size: 2115 1409 226 152 2109 1697
Fragments arranged by size:
2115 2109 1697 1409 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4341 6262 160
Size: 872 3309 1921 1606
Fragments arranged by size:
3309 1921 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
AvaI C'yCGr_G
Cuts at: 1870 1943 3659 4717 4869 5033 1870
Size: 73 1716 1058 152 164 4545
Fragments arranged by size:
4545 1716 1058 164 152 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3712 3745 3712
Size: 33 7675
BamHI G'GATC_C
Cuts at: 5038 5405 5038
Size: 367 7341
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4864 5029 6484 618
Size: 325 217 627 3077 165 1455 1842
Fragments arranged by size:
3077 1842 1455 627 325 217 165
BanII G_rGCy'C
Cuts at: 1391 2058 4062 5027 1391
Size: 667 2004 965 4072
Fragments arranged by size:
4072 2004 965 667
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3107 928
Size: 971 1208 5529
Fragments arranged by size:
5529 1208 971
BbvCI CC'TCA_GC
Cuts at: 4473 4473
Size: 7708
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3337 3371 5899 5933 1903
Size: 34 50 34 1316 34 2528 34 3678
Fragments arranged by size:
3678 2528 1316 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4180 5600 6810 4180
Size: 1420 1210 5078
Fragments arranged by size:
5078 1420 1210
BclI T'GATC_A
Cuts at: 1548 4035 4330 1548
Size: 2487 295 4926
Fragments arranged by size:
4926 2487 295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6319 136
Size: 107 122 71 631 605 4647 1525
Fragments arranged by size:
4647 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2092 2878 3499 7703 2092
Size: 786 621 4204 2097
Fragments arranged by size:
4204 2097 786 621
BlpI GC'TnA_GC
Cuts at: 4320 5125 4320
Size: 805 6903
Bme1580I G_kGCm'C
Cuts at: 1790 4534 5769 6925 1790
Size: 2744 1235 1156 2573
Fragments arranged by size:
2744 2573 1235 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7708
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4758 4777 5439
Size: 416 53 83 399 24 3341 19 662
Cuts at: 5439 6397 442
Size: 958 1753
Fragments arranged by size:
3341 1753 958 662 416 399 83 53
24 19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7708
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3391 3391
Size: 7708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2774 4010 4259 4451 6368 1239
Size: 1535 1236 249 192 1917 2579
Fragments arranged by size:
2579 1917 1535 1236 249 192
Bpu10I CC'TnA_GC
Cuts at: 4473 4473
Size: 7708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2787 4268 5833 6698 6996 2787
Size: 1481 1565 865 298 3499
Fragments arranged by size:
3499 1565 1481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2637 3156 4522 6371 882
Size: 617 1138 519 1366 1849 2219
Fragments arranged by size:
2219 1849 1366 1138 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 2294 5499 493
Size: 711 1090 3205 2702
Fragments arranged by size:
3205 2702 1090 711
BsaBI GATnn'nnATC
Cuts at: 3477 7702 3477
Size: 4225 3483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 3880 5515 5897
Size: 53 83 186 936 2287 60 1635 382
Cuts at: 5897 6532 275
Size: 635 1451
Fragments arranged by size:
2287 1635 1451 936 635 382 186 83
60 53
BsaWI w'CCGG_w
Cuts at: 4524 6141 6539 6813 6960 7371 4524
Size: 1617 398 274 147 411 4861
Fragments arranged by size:
4861 1617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7483
Fragments arranged by size:
7483 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3722 3926 1186
Size: 2536 204 4968
Fragments arranged by size:
4968 2536 204
BseYI C'CCAG_C
Cuts at: 3068 4827 6911 7407 3068
Size: 1759 2084 496 3369
Fragments arranged by size:
3369 2084 1759 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5158 5919 6947 1540
Size: 275 186 3157 761 1028 2301
Fragments arranged by size:
3157 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2410 4062 4263 5769 5854 6925 2058
Size: 352 1652 201 1506 85 1071 2841
Fragments arranged by size:
2841 1652 1506 1071 352 201 85
BsmI GAATG_Cn'
Cuts at: 2695 3851 4447 4700 5219 5312 2695
Size: 1156 596 253 519 93 5091
Fragments arranged by size:
5091 1156 596 519 253 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2410 4062 4263 4534 5027 5769
Size: 399 268 352 1652 201 271 493 742
Cuts at: 5769 5854 6925 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1652 1071 742 493 399 352 271
268 201 85
BspEI T'CCGG_A
Cuts at: 4524 7371 4524
Size: 2847 4861
BspHI T'CATG_A
Cuts at: 5597 7327 5597
Size: 1730 5978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2545 4199 4242 5063 844
Size: 1701 1654 43 821 3489
Fragments arranged by size:
3489 1701 1654 821 43
BsrBI CCG'CTC
Cuts at: 1394 1823 5595 1394
Size: 429 3772 3507
Fragments arranged by size:
3772 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2700 6203 6377 65
Size: 1183 1452 3503 174 1396
Fragments arranged by size:
3503 1452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6352 6539 1700
Size: 110 4542 187 2869
Fragments arranged by size:
4542 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7708
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7708
BssSI C'ACGA_G
Cuts at: 2139 4960 5768 6780 2139
Size: 2821 808 1012 3067
Fragments arranged by size:
3067 2821 1012 808
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2537 2699 3955 794
Size: 1743 162 1256 4547
Fragments arranged by size:
4547 1743 1256 162
BstBI TT'CG_AA
Cuts at: 3688 6512 3688
Size: 2824 4884
BstEII G'GTnAC_C
Cuts at: 1184 4838 1184
Size: 3654 4054
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 4800 2161
Size: 2639 5069
BstYI r'GATC_y
Cuts at: 1972 2071 2092 2333 2878 3235 3256 3499 5038
Size: 99 21 241 545 357 21 243 1539
Cuts at: 5038 5405 5790 5807 7259 7345 7357 7703 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1539 1452 545 385 367 357 346
243 241 99 86 21 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 4695 4695
Size: 7708
BtgI C'CryG_G
Cuts at: 513 1065 2110 2526 2592 4779 4832 513
Size: 552 1045 416 66 2187 53 3389
Fragments arranged by size:
3389 2187 1045 552 416 66 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3464 487
Size: 683 56 2238 4731
Fragments arranged by size:
4731 2238 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 5224 6018 6038 2133
Size: 3091 794 20 3803
Fragments arranged by size:
3803 3091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7673
DraI TTT'AAA
Cuts at: 2832 3201 5012 2832
Size: 369 1811 5528
Fragments arranged by size:
5528 1811 369
DraIII CAC_nnn'GTG
Cuts at: 2405 3172 2405
Size: 767 6941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6715 809
Size: 507 5399 1802
Fragments arranged by size:
5399 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2166 3022
Size: 54 1425 50 275 18 277 59 856
Cuts at: 3022 5155 6044 7673 8
Size: 2133 889 1629 43
Fragments arranged by size:
2133 1629 1425 889 856 277 275 59
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5155 1537
Size: 275 3343 4090
Fragments arranged by size:
4090 3343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2090 2415 2994 3222 3594 4084 4551
Size: 710 27 325 579 228 372 490 467
Cuts at: 4551 5638 1353
Size: 1087 3423
Fragments arranged by size:
3423 1087 710 579 490 467 372 325
228 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3986 4930 6295 6669 6815 387
Size: 1064 138 2397 944 1365 374 146 1280
Fragments arranged by size:
2397 1365 1280 1064 944 374 146 138
EcoICRI GAG'CTC
Cuts at: 2056 4060 2056
Size: 2004 5704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2396 2774 4010 4259 4451 4830
Size: 393 422 342 378 1236 249 192 379
Cuts at: 4830 5771 6368 7155 7423 1239
Size: 941 597 787 268 1524
Fragments arranged by size:
1524 1236 941 787 597 422 393 379
378 342 268 249 192
EcoNI CCTnn'n_nnAGG
Cuts at: 2954 2954
Size: 7708
EcoO109I rG'GnC_Cy
Cuts at: 2038 3898 4760 2038
Size: 1860 862 4986
Fragments arranged by size:
4986 1860 862
EcoRI G'AATT_C
Cuts at: 5017 5395 5017
Size: 378 7330
EcoRV GAT'ATC
Cuts at: 2458 2458
Size: 7708
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3716 3827 3716
Size: 111 7597
FspI TGC'GCA
Cuts at: 6214 6214
Size: 7708
HaeII r_GCGC'y
Cuts at: 1678 2860 6855 1678
Size: 1182 3995 2531
Fragments arranged by size:
3995 2531 1182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 2615 2647 3248
Size: 32 135 32 929 32 82 32 601
Cuts at: 3248 3280 6349 6381 6423 6455 1373
Size: 32 3069 32 42 32 2626
Fragments arranged by size:
3069 2626 929 601 135 82 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3109 5052 5298 669
Size: 988 281 1171 1943 246 3079
Fragments arranged by size:
3079 1943 1171 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2116 4632 4797 748
Size: 1368 2516 165 3659
Fragments arranged by size:
3659 2516 1368 165
HpaI GTT'AAC
Cuts at: 3109 5298 3109
Size: 2189 5519
KpnI G_GTAC'C
Cuts at: 4868 5033 4868
Size: 165 7543
MfeI C'AATT_G
Cuts at: 5307 5307
Size: 7708
MluI A'CGCG_T
Cuts at: 7584 7584
Size: 7708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3028 3988 4587 6822 7006 1305
Size: 1723 960 599 2235 184 2007
Fragments arranged by size:
2235 2007 1723 960 599 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2109 2168 10
Size: 54 1425 343 277 59 5550
Fragments arranged by size:
5550 1425 343 277 59 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3173 5666 6025 6184 518
Size: 648 2007 2493 359 159 2042
Fragments arranged by size:
2493 2042 2007 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4072 4417 5124 5801 6949 7194 1606
Size: 2466 345 707 677 1148 245 2120
Fragments arranged by size:
2466 2120 1148 707 677 345 245
NcoI C'CATG_G
Cuts at: 513 1065 2110 2526 2592 4779 4832 513
Size: 552 1045 416 66 2187 53 3389
Fragments arranged by size:
3389 2187 1045 552 416 66 53
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7708
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7708
NotI GC'GGCC_GC
Cuts at: 5155 5155
Size: 7708
NruI TCG'CGA
Cuts at: 4500 4500
Size: 7708
NspI r_CATG'y
Cuts at: 1132 1229 2649 3076 3953 4118 4999 5066 6611
Size: 97 1420 427 877 165 881 67 1545
Cuts at: 6611 1132
Size: 2229
Fragments arranged by size:
2229 1545 1420 881 877 427 165 97
67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7708
PciI A'CATG_T
Cuts at: 2645 4995 6607 2645
Size: 2350 1612 3746
Fragments arranged by size:
3746 2350 1612
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7708
PfoI T'CCnGG_A
Cuts at: 2955 4756 2955
Size: 1801 5907
PmeI GTTT'AAAC
Cuts at: 5012 5012
Size: 7708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3468 3500 5777 5809 7290 7322 3468
Size: 32 2277 32 1481 32 3854
Fragments arranged by size:
3854 2277 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 3898 4760 2038
Size: 1860 862 4986
Fragments arranged by size:
4986 1860 862
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7708
PsiI TTA'TAA
Cuts at: 5278 5278
Size: 7708
PspXI vC'TCGA_Gb
Cuts at: 4869 4869
Size: 7708
PstI C_TGCA'G
Cuts at: 830 2928 3532 4422 5060 830
Size: 2098 604 890 638 3478
Fragments arranged by size:
3478 2098 890 638 604
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7708
PvuII CAG'CTG
Cuts at: 4072 4417 4072
Size: 345 7363
SacI G_AGCT'C
Cuts at: 2058 4062 2058
Size: 2004 5704
SalI G'TCGA_C
Cuts at: 1936 5050 1936
Size: 3114 4594
SapI GCTCTTCn'nnn_
Cuts at: 2063 2090 2415 2063
Size: 27 325 7356
Fragments arranged by size:
7356 325 27
SbfI CC_TGCA'GG
Cuts at: 5060 5060
Size: 7708
ScaI AGT'ACT
Cuts at: 5956 7668 5956
Size: 1712 5996
SfcI C'TryA_G
Cuts at: 826 1045 2738 2924 3528 4418 5056 6191 6872
Size: 219 1693 186 604 890 638 1135 681
Cuts at: 6872 7063 826
Size: 191 1471
Fragments arranged by size:
1693 1471 1135 890 681 638 604 219
191 186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7708
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7708
SmaI CCC'GGG
Cuts at: 1872 4719 5035 1872
Size: 2847 316 4545
Fragments arranged by size:
4545 2847 316
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2802 3659 4283 4869 5812 6713
Size: 197 926 859 857 624 586 943 901
Cuts at: 6713 6975 820
Size: 262 1553
Fragments arranged by size:
1553 943 926 901 859 857 624 586
262 197
SnaBI TAC'GTA
Cuts at: 493 5499 493
Size: 5006 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7708
SphI G_CATG'C
Cuts at: 1132 3953 5066 1132
Size: 2821 1113 3774
Fragments arranged by size:
3774 2821 1113
SspI AAT'ATT
Cuts at: 5 52 2324 5632 5
Size: 47 2272 3308 2081
Fragments arranged by size:
3308 2272 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7708
StyI C'CwwG_G
Cuts at: 513 1065 2110 2526 2592 4779 4832 5147 513
Size: 552 1045 416 66 2187 53 315 3074
Fragments arranged by size:
3074 2187 1045 552 416 315 66 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4772 5735 5752 5905 6090 6535 325
Size: 1255 3192 963 17 153 185 445 1498
Fragments arranged by size:
3192 1498 1255 963 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4212 5954 7666
Size: 274 80 33 51 1443 2235 1742 1712
Cuts at: 7666 96
Size: 138
Fragments arranged by size:
2235 1742 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4379 4411 7668 7700 4379
Size: 32 3257 32 4387
Fragments arranged by size:
4387 3257 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3902 1316
Size: 2586 5122
XbaI T'CTAG_A
Cuts at: 5044 5044
Size: 7708
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2161 2548 4764 4797 2117
Size: 44 387 2216 33 5028
Fragments arranged by size:
5028 2216 387 44 33
XhoI C'TCGA_G
Cuts at: 1943 3659 4869 1943
Size: 1716 1210 4782
Fragments arranged by size:
4782 1716 1210
XmaI C'CCGG_G
Cuts at: 1870 4717 5033 1870
Size: 2847 316 4545
Fragments arranged by size:
4545 2847 316
XmnI GAAnn'nnTTC
Cuts at: 2084 5837 2084
Size: 3753 3955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 5516 276
Size: 53 83 186 936 2287 1695 2468
Fragments arranged by size:
2468 2287 1695 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI