Restriction map for
pFN21AA1354
(Circular) (Six-base) MAPSORT of: pFN21AA1354.seq Check: 7644 from: 1 to: 6556
pFN21AA1354 6556 bp
With 182 enzymes: SgfI *
March 28, 2012 12:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3899 1937
Size: 1962 4594
Acc65I G'GTAC_C
Cuts at: 2167 3877 2167
Size: 1710 4846
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 2354 2558 3496 3816 3828 4619 6003
Size: 701 21 204 938 320 12 791 1384
Cuts at: 6003 6271 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 938 791 701 320 268 204
21 12
AflII C'TTAA_G
Cuts at: 820 1017 2624 820
Size: 197 1607 4752
Fragments arranged by size:
4752 1607 197
AflIII A'CryG_T
Cuts at: 1203 5455 6432 1203
Size: 4252 977 1327
Fragments arranged by size:
4252 1327 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 3186 1166
Size: 2020 4536
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3045 1184
Size: 1861 4695
AlwNI CAG_nnn'CTG
Cuts at: 2935 4299 5871 2935
Size: 1364 1572 3620
Fragments arranged by size:
3620 1572 1364
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 2806 3865 4091 4243 6352 1493
Size: 1313 1059 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 1059 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 1870
Size: 73 1938 4545
Fragments arranged by size:
4545 1938 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2151 2184 2151
Size: 33 6523
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 3877 5332 618
Size: 325 217 627 380 1710 1455 1842
Fragments arranged by size:
1842 1710 1455 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 3393 3875 1391
Size: 2002 482 4072
Fragments arranged by size:
4072 2002 482
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2688 928
Size: 971 789 4796
Fragments arranged by size:
4796 971 789
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4747 4781 1903
Size: 34 2810 34 3678
Fragments arranged by size:
3678 2810 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 2125 1548
Size: 577 5979
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5167 136
Size: 107 122 71 631 605 3495 1525
Fragments arranged by size:
3495 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2987 6551 2987
Size: 3564 2992
BlpI GC'TnA_GC
Cuts at: 3973 3973
Size: 6556
Bme1580I G_kGCm'C
Cuts at: 1790 2977 3193 4617 5773 1790
Size: 1187 216 1424 1156 2573
Fragments arranged by size:
2573 1424 1187 1156 216
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2574 4287 5245
Size: 416 53 83 399 24 1157 1713 958
Cuts at: 5245 442
Size: 1753
Fragments arranged by size:
1753 1713 1157 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2394 2826 1056
Size: 1338 432 4786
Fragments arranged by size:
4786 1338 432
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3503
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5216 1239
Size: 3977 2579
Bpu10I CC'TnA_GC
Cuts at: 2672 2672
Size: 6556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3205 4681 5546 5844 3205
Size: 1476 865 298 3917
Fragments arranged by size:
3917 1476 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5219 882
Size: 617 3720 2219
Fragments arranged by size:
3720 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 4347 493
Size: 711 1560 1583 2702
Fragments arranged by size:
2702 1583 1560 711
BsaBI GATnn'nnATC
Cuts at: 6550 6550
Size: 6556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 2216 4363 4745
Size: 53 83 186 936 504 179 2147 382
Cuts at: 4745 5380 275
Size: 635 1451
Fragments arranged by size:
2147 1451 936 635 504 382 186 179
83 53
BsaWI w'CCGG_w
Cuts at: 4989 5387 5661 5808 6219 4989
Size: 398 274 147 411 5326
Fragments arranged by size:
5326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3612 3642 1178
Size: 30 165 30 2209 30 4092
Fragments arranged by size:
4092 2209 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2931 1186
Size: 1745 4811
BseYI C'CCAG_C
Cuts at: 5759 6255 5759
Size: 496 6060
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2786 3249 1163
Size: 573 1050 463 4470
Fragments arranged by size:
4470 1050 573 463
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3393 4617 4702 5773 3393
Size: 1224 85 1071 4176
Fragments arranged by size:
4176 1224 1071 85
BsmI GAATG_Cn'
Cuts at: 3762 4067 4160 3762
Size: 305 93 6158
Fragments arranged by size:
6158 305 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2044 2044
Size: 6556
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2977 3193 3393 3875 4617 4702 5773
Size: 399 1187 216 200 482 742 85 1071
Cuts at: 5773 1391
Size: 2174
Fragments arranged by size:
2174 1187 1071 742 482 399 216 200
85
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 2781 4445 6175 2781
Size: 1664 1730 3162
Fragments arranged by size:
3162 1730 1664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 3136 3911 844
Size: 1963 329 775 3489
Fragments arranged by size:
3489 1963 775 329
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3460 5051 5225 65
Size: 1183 2212 1591 174 1396
Fragments arranged by size:
2212 1591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5200 5387 1700
Size: 110 3390 187 2869
Fragments arranged by size:
3390 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3470 3773 96
Size: 3374 303 2879
Fragments arranged by size:
3374 2879 303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 4616 5628 4616
Size: 1012 5544
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6556
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 3810 1184
Size: 2626 3930
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 2902
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 2749 2987 3886 4253 4638 4655 6107 6193
Size: 777 238 899 367 385 17 1452 86
Cuts at: 6193 6205 6551 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 899 777 385 367 346 238
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5817
Fragments arranged by size:
5817 683 56
BtsI GCAGTG_nn'
Cuts at: 3232 4072 4866 4886 3232
Size: 840 794 20 4902
Fragments arranged by size:
4902 840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DraIII CAC_nnn'GTG
Cuts at: 3758 3758
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3243 4003 4892
Size: 54 1425 50 275 18 1413 760 889
Cuts at: 4892 6521 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1413 889 760 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4486 1353
Size: 3133 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5143 5517 5663 387
Size: 1064 138 3554 374 146 1280
Fragments arranged by size:
3554 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3391 3391
Size: 6556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2354 2558 3496 3816 3828 4619
Size: 393 701 21 204 938 320 12 791
Cuts at: 4619 5216 6003 6271 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 938 791 787 701 597 393 320
268 204 21 12
EcoRI G'AATT_C
Cuts at: 3865 4243 3865
Size: 378 6178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2033 2165 2453 2033
Size: 132 288 6136
Fragments arranged by size:
6136 288 132
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2172 2204 3612 3644 5197
Size: 32 135 32 600 32 1408 32 1553
Cuts at: 5197 5229 5271 5303 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1553 1408 600 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3900 4146 669
Size: 988 281 1962 246 3079
Fragments arranged by size:
3079 1962 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2285 748
Size: 1537 5019
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 2171 3881 2171
Size: 1710 4846
MfeI C'AATT_G
Cuts at: 3585 4155 3585
Size: 570 5986
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2455 2658 3457 3598 5670 5854 1305
Size: 1150 203 799 141 2072 184 2007
Fragments arranged by size:
2072 2007 1150 799 203 184 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3245 10
Size: 54 1425 343 1413 3321
Fragments arranged by size:
3321 1425 1413 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2980 3186 3207 4514 4873 5032
Size: 648 843 971 206 21 1307 359 159
Cuts at: 5032 518
Size: 2042
Fragments arranged by size:
2042 1307 971 843 648 359 206 159
21
MspA1I CmG'CkG
Cuts at: 1606 2935 3233 3972 4649 5797 6042 1606
Size: 1329 298 739 677 1148 245 2120
Fragments arranged by size:
2120 1329 1148 739 677 298 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6004
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 2390 2822 1052
Size: 1338 432 4786
Fragments arranged by size:
4786 1338 432
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 3469 3914 5459 1132
Size: 97 2240 445 1545 2229
Fragments arranged by size:
2240 2229 1545 445 97
PasI CC'CwG_GG
Cuts at: 1348 3806 1348
Size: 2458 4098
PciI A'CATG_T
Cuts at: 5455 5455
Size: 6556
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2177 1108
Size: 1069 5487
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2631 2663 4625 4657 6138 6170 2631
Size: 32 1962 32 1481 32 3017
Fragments arranged by size:
3017 1962 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3446 1613
Size: 1833 4723
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2779 2811 2779
Size: 32 6524
PstI C_TGCA'G
Cuts at: 830 3908 830
Size: 3078 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
PvuII CAG'CTG
Cuts at: 2935 3233 2935
Size: 298 6258
SacI G_AGCT'C
Cuts at: 3393 3393
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 2563 4804 6516 2563
Size: 2241 1712 2603
Fragments arranged by size:
2603 2241 1712
SexAI A'CCwGG_T
Cuts at: 2170 2170
Size: 6556
SfcI C'TryA_G
Cuts at: 826 1045 3444 3904 5039 5720 5911 826
Size: 219 2399 460 1135 681 191 1471
Fragments arranged by size:
2399 1471 1135 681 460 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 3184 4660 5561 5823 820
Size: 197 926 681 560 1476 901 262 1553
Fragments arranged by size:
1553 1476 926 901 681 560 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 2406 2722 4480 5
Size: 47 2354 316 1758 2081
Fragments arranged by size:
2354 2081 1758 316 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6556
StyI C'CwwG_G
Cuts at: 513 1065 2017 3995 513
Size: 552 952 1978 3074
Fragments arranged by size:
3074 1978 952 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4583 4600 4753 4938 5383 325
Size: 1255 3003 17 153 185 445 1498
Fragments arranged by size:
3003 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2561 3470 3773
Size: 274 80 33 51 1443 584 909 303
Cuts at: 3773 4802 6514 96
Size: 1029 1712 138
Fragments arranged by size:
1712 1443 1029 909 584 303 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3772 3804 6516 6548 3772
Size: 32 2712 32 3780
Fragments arranged by size:
3780 2712 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3818 3892 3818
Size: 74 6482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3614 3614
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 4685 4685
Size: 6556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI