Restriction map for
pFN21AA1351
(Circular) (Six-base) MAPSORT of: pFN21AA1351.seq Check: 4162 from: 1 to: 8377
pFN21AA1351 8377 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6187 278
Size: 53 83 186 936 4651 2468
Fragments arranged by size:
4651 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2920 5720 1937
Size: 983 2800 4594
Fragments arranged by size:
4594 2800 983
Acc65I G'GTAC_C
Cuts at: 5698 5698
Size: 8377
AclI AA'CG_TT
Cuts at: 1563 6504 6877 1563
Size: 4941 373 3063
Fragments arranged by size:
4941 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2732 3064 4347 6440 7824 8092 1632
Size: 1100 332 1283 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 1283 1100 332 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8180
AflIII A'CryG_T
Cuts at: 1203 2781 2947 7276 8253 1203
Size: 1578 166 4329 977 1327
Fragments arranged by size:
4329 1578 1327 977 166
AgeI A'CCGG_T
Cuts at: 7208 7208
Size: 8377
AhdI GACnn_n'nnGTC
Cuts at: 7106 7106
Size: 8377
AleI CACnn'nnGTG
Cuts at: 1166 3549 1166
Size: 2383 5994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5257 1184
Size: 4073 4304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3181 3213 3181
Size: 32 8345
AlwNI CAG_nnn'CTG
Cuts at: 2594 3463 5017 5053 6120 7692 2594
Size: 869 1554 36 1067 1572 3279
Fragments arranged by size:
3279 1572 1554 1067 869 36
ApaI G_GGCC'C
Cuts at: 2059 2059
Size: 8377
ApaLI G'TGCA_C
Cuts at: 3353 6434 7590 3353
Size: 3081 1156 4140
Fragments arranged by size:
4140 3081 1156
ApoI r'AATT_y
Cuts at: 1493 2691 2723 3181 3240 4195 4201 4678 4721
Size: 1198 32 458 59 955 6 477 43
Cuts at: 4721 4937 5686 5912 6064 8173 1493
Size: 216 749 226 152 2109 1697
Fragments arranged by size:
2109 1697 1198 955 749 477 458 226
216 152 59 43 32 6
AseI AT'TA_AT
Cuts at: 160 1032 4301 6931 160
Size: 872 3269 2630 1606
Fragments arranged by size:
3269 2630 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
AvaI C'yCGr_G
Cuts at: 1870 1943 2799 3906 4029 4869 5702 1870
Size: 73 856 1107 123 840 833 4545
Fragments arranged by size:
4545 1107 856 840 833 123 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4158 4191 4158
Size: 33 8344
BamHI G'GATC_C
Cuts at: 5707 6074 5707
Size: 367 8010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 2663 4530 4740 5698
Size: 325 217 627 663 213 1867 210 958
Cuts at: 5698 7153 618
Size: 1455 1842
Fragments arranged by size:
1867 1842 1455 958 663 627 325 217
213 210
BanII G_rGCy'C
Cuts at: 1391 2059 5696 1391
Size: 668 3637 4072
Fragments arranged by size:
4072 3637 668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6568 6602 1903
Size: 34 4631 34 3678
Fragments arranged by size:
4631 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4237 4330 6269 7479 4237
Size: 93 1939 1210 5135
Fragments arranged by size:
5135 1939 1210 93
BclI T'GATC_A
Cuts at: 1548 3972 4987 1548
Size: 2424 1015 4938
Fragments arranged by size:
4938 2424 1015
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6988 136
Size: 107 122 71 631 605 5316 1525
Fragments arranged by size:
5316 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2957 8372 2957
Size: 5415 2962
BlpI GC'TnA_GC
Cuts at: 5794 5794
Size: 8377
Bme1580I G_kGCm'C
Cuts at: 1790 2059 3357 4535 6438 7594 1790
Size: 269 1298 1178 1903 1156 2573
Fragments arranged by size:
2573 1903 1298 1178 1156 269
BmgBI CAC'GTC
Cuts at: 1318 4909 1318
Size: 3591 4786
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 6108 7066
Size: 416 53 83 399 24 681 4010 958
Cuts at: 7066 442
Size: 1753
Fragments arranged by size:
4010 1753 958 681 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5612 5612
Size: 8377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3800 7037 1239
Size: 2561 3237 2579
Fragments arranged by size:
3237 2579 2561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2688 2843 3143 3344 5471 5666 6502 7367 7665
Size: 155 300 201 2127 195 836 865 298
Cuts at: 7665 2688
Size: 3400
Fragments arranged by size:
3400 2127 865 836 300 298 201 195
155
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7040 882
Size: 617 5541 2219
Fragments arranged by size:
5541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2948 6168 493
Size: 711 1744 3220 2702
Fragments arranged by size:
3220 2702 1744 711
BsaBI GATnn'nnATC
Cuts at: 2810 3003 8371 2810
Size: 193 5368 2816
Fragments arranged by size:
5368 2816 193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5238 6184 6566 7201
Size: 53 83 186 936 3705 946 382 635
Cuts at: 7201 275
Size: 1451
Fragments arranged by size:
3705 1451 946 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6810 7208 7482 7629 8040 6810
Size: 398 274 147 411 7147
Fragments arranged by size:
7147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4314 4344 4834 4864 1178
Size: 30 165 30 2911 30 490 30 4691
Fragments arranged by size:
4691 2911 490 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4652 4857 5327 5387 1186
Size: 3466 205 470 60 4176
Fragments arranged by size:
4176 3466 470 205 60
BseYI C'CCAG_C
Cuts at: 2632 3314 7580 8076 2632
Size: 682 4266 496 2933
Fragments arranged by size:
4266 2933 682 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4766 4796 5066 5088 1163
Size: 573 3030 30 270 22 4452
Fragments arranged by size:
4452 3030 573 270 30 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5827 6588 7616 1540
Size: 275 186 3826 761 1028 2301
Fragments arranged by size:
3826 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2433 3357 4111 4914 5150 6438 6523 7594 2433
Size: 924 754 803 236 1288 85 1071 3216
Fragments arranged by size:
3216 1288 1071 924 803 754 236 85
BsmI GAATG_Cn'
Cuts at: 2443 3115 5888 5981 2443
Size: 672 2773 93 4839
Fragments arranged by size:
4839 2773 672 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5423 5423
Size: 8377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2433 3357 4111 4535 4914 5150
Size: 399 269 374 924 754 424 379 236
Cuts at: 5150 5696 6438 6523 7594 1391
Size: 546 742 85 1071 2174
Fragments arranged by size:
2174 1071 924 754 742 546 424 399
379 374 269 236 85
BspEI T'CCGG_A
Cuts at: 8040 8040
Size: 8377
BspHI T'CATG_A
Cuts at: 3144 6266 7996 3144
Size: 3122 1730 3525
Fragments arranged by size:
3525 3122 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2769 4154 4818 5259 5732 844
Size: 1925 1385 664 441 473 3489
Fragments arranged by size:
3489 1925 1385 664 473 441
BsrBI CCG'CTC
Cuts at: 1394 1823 6264 1394
Size: 429 4441 3507
Fragments arranged by size:
4441 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3675 3706 4314 6872 7046 65
Size: 1183 2427 31 608 2558 174 1396
Fragments arranged by size:
2558 2427 1396 1183 608 174 31
BsrFI r'CCGG_y
Cuts at: 1700 1810 7021 7208 1700
Size: 110 5211 187 2869
Fragments arranged by size:
5211 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4311 5003 96
Size: 4215 692 3470
Fragments arranged by size:
4215 3470 692
BssHII G'CGCG_C
Cuts at: 1925 2039 1925
Size: 114 8263
BssSI C'ACGA_G
Cuts at: 6437 7449 6437
Size: 1012 7365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5552 794
Size: 4758 3619
BstBI TT'CG_AA
Cuts at: 2953 7181 2953
Size: 4228 4149
BstEII G'GTnAC_C
Cuts at: 1184 3858 1184
Size: 2674 5703
BstXI CCAn_nnnn'nTGG
Cuts at: 2237 5158 2237
Size: 2921 5456
BstYI r'GATC_y
Cuts at: 1972 2328 2957 2998 3004 5707 6074 6459 6476
Size: 356 629 41 6 2703 367 385 17
Cuts at: 6476 7928 8014 8026 8372 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2703 1977 1452 629 385 367 356 346
86 41 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 2587 5310 2587
Size: 2723 5654
BtgI C'CryG_G
Cuts at: 513 1065 3915 513
Size: 552 2850 4975
Fragments arranged by size:
4975 2850 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4135 487
Size: 683 56 2909 4729
Fragments arranged by size:
4729 2909 683 56
BtsI GCAGTG_nn'
Cuts at: 3461 5532 5893 6687 6707 3461
Size: 2071 361 794 20 5131
Fragments arranged by size:
5131 2071 794 361 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8342
DraI TTT'AAA
Cuts at: 4200 5681 4200
Size: 1481 6896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7384 809
Size: 507 6068 1802
Fragments arranged by size:
6068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2649 4060 5824
Size: 54 1425 50 275 18 819 1411 1764
Cuts at: 5824 6713 8342 8
Size: 889 1629 43
Fragments arranged by size:
1764 1629 1425 1411 889 819 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5824 1537
Size: 275 4012 4090
Fragments arranged by size:
4090 4012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2462 3872 5667 6307 1353
Size: 816 293 1410 1795 640 3423
Fragments arranged by size:
3423 1795 1410 816 640 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6964 7338 7484 387
Size: 1064 138 5375 374 146 1280
Fragments arranged by size:
5375 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2732 3064 3800 4347 6440 7037 7824
Size: 393 1100 332 736 547 2093 597 787
Cuts at: 7824 8092 1239
Size: 268 1524
Fragments arranged by size:
2093 1524 1100 787 736 597 547 393
332 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2586 4284 2586
Size: 1698 6679
EcoO109I rG'GnC_Cy
Cuts at: 2055 2056 2591 4554 5135 2055
Size: 1 535 1963 581 5297
Fragments arranged by size:
5297 1963 581 535 1
EcoRI G'AATT_C
Cuts at: 2723 3240 4678 4721 5686 6064 2723
Size: 517 1438 43 965 378 5036
Fragments arranged by size:
5036 1438 965 517 378 43
FspI TGC'GCA
Cuts at: 6883 6883
Size: 8377
HaeII r_GCGC'y
Cuts at: 1678 7524 1678
Size: 5846 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5633 5665 7018 7050 7092
Size: 32 135 32 4061 32 1353 32 42
Cuts at: 7092 7124 1373
Size: 32 2626
Fragments arranged by size:
4061 2626 1353 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 4515 4630 4980 5344 5721
Size: 988 281 1494 1083 115 350 364 377
Cuts at: 5721 5967 669
Size: 246 3079
Fragments arranged by size:
3079 1494 1083 988 377 364 350 281
246 115
HindIII A'AGCT_T
Cuts at: 748 5478 748
Size: 4730 3647
HpaI GTT'AAC
Cuts at: 4515 5967 4515
Size: 1452 6925
KpnI G_GTAC'C
Cuts at: 5702 5702
Size: 8377
MfeI C'AATT_G
Cuts at: 3309 5976 3309
Size: 2667 5710
MluI A'CGCG_T
Cuts at: 8253 8253
Size: 8377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3686 5037 7491 7675 1305
Size: 2381 1351 2454 184 2007
Fragments arranged by size:
2454 2381 2007 1351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2651 4062 10
Size: 54 1425 343 819 1411 4325
Fragments arranged by size:
4325 1425 1411 819 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2928 3449 3549 3719 6335 6694 6853
Size: 648 1762 521 100 170 2616 359 159
Cuts at: 6853 518
Size: 2042
Fragments arranged by size:
2616 2042 1762 648 521 359 170 159
100
MspA1I CmG'CkG
Cuts at: 1606 5621 5793 6470 7618 7863 1606
Size: 4015 172 677 1148 245 2120
Fragments arranged by size:
4015 2120 1148 677 245 172
NcoI C'CATG_G
Cuts at: 513 1065 3915 513
Size: 552 2850 4975
Fragments arranged by size:
4975 2850 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8377
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8377
NotI GC'GGCC_GC
Cuts at: 5824 5824
Size: 8377
NspI r_CATG'y
Cuts at: 1132 1229 2342 2785 5735 7280 1132
Size: 97 1113 443 2950 1545 2229
Fragments arranged by size:
2950 2229 1545 1113 443 97
PasI CC'CwG_GG
Cuts at: 1348 2594 1348
Size: 1246 7131
PciI A'CATG_T
Cuts at: 2781 7276 2781
Size: 4495 3882
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2460 4124 1108
Size: 1352 1664 5361
Fragments arranged by size:
5361 1664 1352
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 8377
PmeI GTTT'AAAC
Cuts at: 5681 5681
Size: 8377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6446 6478 7959 7991 6446
Size: 32 1481 32 6832
Fragments arranged by size:
6832 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3932 1613
Size: 2319 6058
PsiI TTA'TAA
Cuts at: 2964 5947 2964
Size: 2983 5394
PspOMI G'GGCC_C
Cuts at: 2055 2055
Size: 8377
PstI C_TGCA'G
Cuts at: 830 3169 3987 5236 5256 5729 830
Size: 2339 818 1249 20 473 3478
Fragments arranged by size:
3478 2339 1249 818 473 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8377
PvuII CAG'CTG
Cuts at: 5621 5621
Size: 8377
SalI G'TCGA_C
Cuts at: 1936 5719 1936
Size: 3783 4594
SapI GCTCTTCn'nnn_
Cuts at: 2462 3872 2462
Size: 1410 6967
SbfI CC_TGCA'GG
Cuts at: 5729 5729
Size: 8377
ScaI AGT'ACT
Cuts at: 6625 8337 6625
Size: 1712 6665
SfcI C'TryA_G
Cuts at: 826 1045 3165 3246 3257 3983 4961 5232 5252
Size: 219 2120 81 11 726 978 271 20
Cuts at: 5252 5725 6860 7541 7732 826
Size: 473 1135 681 191 1471
Fragments arranged by size:
2120 1471 1135 978 726 681 473 271
219 191 81 20 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8377
SmaI CCC'GGG
Cuts at: 1872 4031 5704 1872
Size: 2159 1673 4545
Fragments arranged by size:
4545 2159 1673
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2703 2799 2822 3158 3359 3906
Size: 197 926 760 96 23 336 201 547
Cuts at: 3906 4869 5486 5645 6481 7382 7644 820
Size: 963 617 159 836 901 262 1553
Fragments arranged by size:
1553 963 926 901 836 760 617 547
336 262 201 197 159 96 23
SnaBI TAC'GTA
Cuts at: 493 6168 493
Size: 5675 2702
SpeI A'CTAG_T
Cuts at: 152 3584 152
Size: 3432 4945
SphI G_CATG'C
Cuts at: 1132 2342 5735 1132
Size: 1210 3393 3774
Fragments arranged by size:
3774 3393 1210
SspI AAT'ATT
Cuts at: 5 52 6301 5
Size: 47 6249 2081
Fragments arranged by size:
6249 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8377
StyI C'CwwG_G
Cuts at: 513 1065 3915 4550 4592 5655 5816 513
Size: 552 2850 635 42 1063 161 3074
Fragments arranged by size:
3074 2850 1063 635 552 161 42
SwaI ATTT'AAAT
Cuts at: 4200 4200
Size: 8377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2831 4246 4440 6404 6421 6574 6759
Size: 1255 1251 1415 194 1964 17 153 185
Cuts at: 6759 7204 325
Size: 445 1498
Fragments arranged by size:
1964 1498 1415 1255 1251 445 194 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4311 5003 6623
Size: 274 80 33 51 1443 2334 692 1620
Cuts at: 6623 8335 96
Size: 1712 138
Fragments arranged by size:
2334 1712 1620 1443 692 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 2854 2886 4314 4346 4805 4834 4837
Size: 32 474 32 1428 32 459 29 3
Cuts at: 4837 4866 8337 8369 2348
Size: 29 3471 32 2356
Fragments arranged by size:
3471 2356 1428 474 459 32 32 32
32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8377
XbaI T'CTAG_A
Cuts at: 5713 5713
Size: 8377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3512 4595 5158 5188 3512
Size: 1083 563 30 6701
Fragments arranged by size:
6701 1083 563 30
XhoI C'TCGA_G
Cuts at: 1943 2799 3906 4869 1943
Size: 856 1107 963 5451
Fragments arranged by size:
5451 1107 963 856
XmaI C'CCGG_G
Cuts at: 1870 4029 5702 1870
Size: 2159 1673 4545
Fragments arranged by size:
4545 2159 1673
XmnI GAAnn'nnTTC
Cuts at: 4574 6506 4574
Size: 1932 6445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6185 276
Size: 53 83 186 936 4651 2468
Fragments arranged by size:
4651 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI