Restriction map for
pFN21AA1343
(Circular) (Six-base) MAPSORT of: pFN21AA1343.seq Check: 305 from: 1 to: 6190
pFN21AA1343 6190 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4253 5637 5905 1632
Size: 2621 1384 268 1917
Fragments arranged by size:
2621 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 5089 6066 1203
Size: 3886 977 1327
Fragments arranged by size:
3886 1327 977
AgeI A'CCGG_T
Cuts at: 3412 5021 3412
Size: 1609 4581
AhdI GACnn_n'nnGTC
Cuts at: 3177 4919 3177
Size: 1742 4448
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2553 2585 2553
Size: 32 6158
AlwNI CAG_nnn'CTG
Cuts at: 3933 5505 3933
Size: 1572 4618
ApaLI G'TGCA_C
Cuts at: 2796 4247 5403 2796
Size: 1451 1156 3583
Fragments arranged by size:
3583 1451 1156
ApoI r'AATT_y
Cuts at: 1493 2042 2831 3459 3499 3725 3877 5986 1493
Size: 549 789 628 40 226 152 2109 1697
Fragments arranged by size:
2109 1697 789 628 549 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 2862 4744 160
Size: 872 1830 1882 1606
Fragments arranged by size:
1882 1830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 3515 1870
Size: 73 1572 4545
Fragments arranged by size:
4545 1572 73
BamHI G'GATC_C
Cuts at: 2034 3520 3887 2034
Size: 1486 367 4337
Fragments arranged by size:
4337 1486 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3511 4966 618
Size: 325 217 627 1724 1455 1842
Fragments arranged by size:
1842 1724 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3509 1391
Size: 2118 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3302 3336 4381 4415 1903
Size: 34 1365 34 1045 34 3678
Fragments arranged by size:
3678 1365 1045 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2126 4082 5292 2126
Size: 1956 1210 3024
Fragments arranged by size:
3024 1956 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4801 136
Size: 107 122 71 631 605 3129 1525
Fragments arranged by size:
3129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 2781 3607 2781
Size: 826 5364
Bme1580I G_kGCm'C
Cuts at: 1790 2800 4251 5407 1790
Size: 1010 1451 1156 2573
Fragments arranged by size:
2573 1451 1156 1010
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3185 3921 4879
Size: 416 53 83 399 24 1768 736 958
Cuts at: 4879 442
Size: 1753
Fragments arranged by size:
1768 1753 958 736 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3293 4850 1239
Size: 2054 1557 2579
Fragments arranged by size:
2579 2054 1557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4315 5180 5478 4315
Size: 865 298 5027
Fragments arranged by size:
5027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2106 4853 882
Size: 617 607 2747 2219
Fragments arranged by size:
2747 2219 617 607
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 2523 6184 2523
Size: 3661 2529
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3001 3997 4379 5014
Size: 53 83 186 936 1468 996 382 635
Cuts at: 5014 275
Size: 1451
Fragments arranged by size:
1468 1451 996 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2260 3412 4623 5021 5295 5442 5853 2260
Size: 1152 1211 398 274 147 411 2597
Fragments arranged by size:
2597 1211 1152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5965
Fragments arranged by size:
5965 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2897 3032 3104 3156 3158 3291 1186
Size: 1711 135 72 52 2 133 4085
Fragments arranged by size:
4085 1711 135 133 72 52 2
BseYI C'CCAG_C
Cuts at: 5393 5889 5393
Size: 496 5694
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3470 1163
Size: 573 1734 3883
Fragments arranged by size:
3883 1734 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2800 3131 4251 4336 5407 2800
Size: 331 1120 85 1071 3583
Fragments arranged by size:
3583 1120 1071 331 85
BsmI GAATG_Cn'
Cuts at: 2782 3701 3794 2782
Size: 919 93 5178
Fragments arranged by size:
5178 919 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2800 3131 3509 4251 4336 5407 1391
Size: 399 1010 331 378 742 85 1071 2174
Fragments arranged by size:
2174 1071 1010 742 399 378 331 85
BspEI T'CCGG_A
Cuts at: 2260 5853 2260
Size: 3593 2597
BspHI T'CATG_A
Cuts at: 4079 5809 4079
Size: 1730 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3545 844
Size: 2701 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 4685 4859 65
Size: 1183 894 2543 174 1396
Fragments arranged by size:
2543 1396 1183 894 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3291 3412 4834 5021 1700
Size: 110 1481 121 1422 187 2869
Fragments arranged by size:
2869 1481 1422 187 121 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 4250 5262 4250
Size: 1012 5178
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2034 3520 3887 4272 4289 5741 5827 5839
Size: 62 1486 367 385 17 1452 86 12
Cuts at: 5839 6185 1972
Size: 346 1977
Fragments arranged by size:
1977 1486 1452 385 367 346 86 62
17 12
Bsu36I CC'TnA_GG
Cuts at: 2658 2729 2658
Size: 71 6119
BtgI C'CryG_G
Cuts at: 513 1065 3423 513
Size: 552 2358 3280
Fragments arranged by size:
3280 2358 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2606 487
Size: 683 56 1380 4071
Fragments arranged by size:
4071 1380 683 56
BtsI GCAGTG_nn'
Cuts at: 2904 3266 3482 3706 4500 4520 2904
Size: 362 216 224 794 20 4574
Fragments arranged by size:
4574 794 362 224 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 3348 3494 3348
Size: 146 6044
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2968 3338 3637
Size: 54 1425 50 275 18 1138 370 299
Cuts at: 3637 4526 6155 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 370 299 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2593 3119 3135 3291 4120 1353
Size: 1240 526 16 156 829 3423
Fragments arranged by size:
3423 1240 829 526 156 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4777 5151 5297 387
Size: 1064 138 3188 374 146 1280
Fragments arranged by size:
3188 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3293 4253 4850 5637 5905 1239
Size: 393 1661 960 597 787 268 1524
Fragments arranged by size:
1661 1524 960 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2831 3459 3499 3877 2831
Size: 628 40 378 5144
Fragments arranged by size:
5144 628 378 40
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3001 3001
Size: 6190
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 5337 1678
Size: 3659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3163 3195 3461 3493 4831
Size: 32 135 32 1591 32 266 32 1338
Cuts at: 4831 4863 4905 4937 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1591 1338 266 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3437 3534 3780 669
Size: 988 281 1499 97 246 3079
Fragments arranged by size:
3079 1499 988 281 246 97
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 2148 2361 3789 2148
Size: 213 1428 4549
Fragments arranged by size:
4549 1428 213
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1993 2852 3233 3287 5304 5488 1305
Size: 688 859 381 54 2017 184 2007
Fragments arranged by size:
2017 2007 859 688 381 184 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2970 10
Size: 54 1425 343 1138 3230
Fragments arranged by size:
3230 1425 1138 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4148 4507 4666 518
Size: 648 2982 359 159 2042
Fragments arranged by size:
2982 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3606 4283 5431 5676 1606
Size: 2000 677 1148 245 2120
Fragments arranged by size:
2120 2000 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5638
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2197 2668 3548 5093 1132
Size: 97 968 471 880 1545 2229
Fragments arranged by size:
2229 1545 968 880 471 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 5089 5089
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2504 1108
Size: 1396 4794
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PstI C_TGCA'G
Cuts at: 830 3542 830
Size: 2712 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 3119 3291 3119
Size: 172 6018
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2701 2995 3538 4673 5354 5545 826
Size: 219 1656 294 543 1135 681 191 1471
Fragments arranged by size:
1656 1471 1135 681 543 294 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3517 1872
Size: 1645 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4294 5195 5457 820
Size: 197 926 2351 901 262 1553
Fragments arranged by size:
2351 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3548 1132
Size: 2416 3774
SspI AAT'ATT
Cuts at: 5 52 2381 4114 5
Size: 47 2329 1733 2081
Fragments arranged by size:
2329 2081 1733 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 3629 513
Size: 552 2564 3074
Fragments arranged by size:
3074 2564 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4217 4234 4387 4572 5017 325
Size: 1255 2637 17 153 185 445 1498
Fragments arranged by size:
2637 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2064 4436 6148
Size: 274 80 33 51 1443 87 2372 1712
Cuts at: 6148 96
Size: 138
Fragments arranged by size:
2372 1712 1443 274 138 87 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2242 2274 2500 2532 6150 6182 2242
Size: 32 226 32 3618 32 2250
Fragments arranged by size:
3618 2250 226 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3062 3062
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3515 1870
Size: 1645 4545
XmnI GAAnn'nnTTC
Cuts at: 2981 4319 2981
Size: 1338 4852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI