Restriction map for
pFN21AA1342
(Circular) (Six-base) MAPSORT of: pFN21AA1342.seq Check: 9957 from: 1 to: 5980
pFN21AA1342 5980 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2050 2427 2826 2844 4043 5427 5695 1632
Size: 418 377 399 18 1199 1384 268 1917
Fragments arranged by size:
1917 1384 1199 418 399 377 268 18
AflII C'TTAA_G
Cuts at: 820 1017 2174 820
Size: 197 1157 4626
Fragments arranged by size:
4626 1157 197
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 2054 1166
Size: 888 5092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2128 1184
Size: 944 5036
AlwNI CAG_nnn'CTG
Cuts at: 3723 5295 3723
Size: 1572 4408
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2033 2272 3122 3289 3515 3667 5776 1493
Size: 540 239 850 167 226 152 2109 1697
Fragments arranged by size:
2109 1697 850 540 239 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2788 3305 1870
Size: 73 845 517 4545
Fragments arranged by size:
4545 845 517 73
BamHI G'GATC_C
Cuts at: 2650 3310 3677 2650
Size: 660 367 4953
Fragments arranged by size:
4953 660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3301 4756 618
Size: 325 217 627 1514 1455 1842
Fragments arranged by size:
1842 1514 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3299 1391
Size: 1908 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2077 2850 3035 928
Size: 971 178 773 185 3873
Fragments arranged by size:
3873 971 773 185 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2679 3872 5082 2679
Size: 1193 1210 3577
Fragments arranged by size:
3577 1210 1193
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2302 3225 5975 2302
Size: 923 2750 2307
Fragments arranged by size:
2750 2307 923
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 4041 5197 1790
Size: 2251 1156 2573
Fragments arranged by size:
2573 2251 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 4640 1239
Size: 1139 2262 2579
Fragments arranged by size:
2579 2262 1139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4970 5268 4105
Size: 865 298 4817
Fragments arranged by size:
4817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2660 4643 882
Size: 617 1161 1983 2219
Fragments arranged by size:
2219 1983 1161 617
BsaAI yAC'GTr
Cuts at: 493 1204 3265 3771 493
Size: 711 2061 506 2702
Fragments arranged by size:
2702 2061 711 506
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2963 4413 4811 5085 5232 5643 2963
Size: 1450 398 274 147 411 3300
Fragments arranged by size:
3300 1450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5755
Fragments arranged by size:
5755 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2139 1186
Size: 953 5027
BseYI C'CCAG_C
Cuts at: 2464 5183 5679 2464
Size: 2719 496 2765
Fragments arranged by size:
2765 2719 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3207 1163
Size: 573 1471 3936
Fragments arranged by size:
3936 1471 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3216 3270 4041 4126 5197 3216
Size: 54 771 85 1071 3999
Fragments arranged by size:
3999 1071 771 85 54
BsmI GAATG_Cn'
Cuts at: 2368 3491 3584 2368
Size: 1123 93 4764
Fragments arranged by size:
4764 1123 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3216 3270 3299 4041 4126 5197 1391
Size: 399 1426 54 29 742 85 1071 2174
Fragments arranged by size:
2174 1426 1071 742 399 85 54 29
BspEI T'CCGG_A
Cuts at: 2963 5643 2963
Size: 2680 3300
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2276 4475 4649 65
Size: 1183 1028 2199 174 1396
Fragments arranged by size:
2199 1396 1183 1028 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2061 2109 2287 2302 2650 2974 3225 3310
Size: 89 48 178 15 348 324 251 85
Cuts at: 3310 3677 4062 4079 5531 5617 5629 5975 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 348 346 324 251
178 89 86 85 48 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2536 2550 513
Size: 552 939 532 14 3943
Fragments arranged by size:
3943 939 552 532 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 3066 3496 4290 4310 3066
Size: 430 794 20 4736
Fragments arranged by size:
4736 794 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 2421 3284 2421
Size: 863 5117
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945
Size: 54 1425 50 275 18 1597 889 1629
Cuts at: 5945 8
Size: 43
Fragments arranged by size:
1629 1597 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 2480 3910 1353
Size: 1089 38 1430 3423
Fragments arranged by size:
3423 1430 1089 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2378 2427 2826 2844 4043 4640
Size: 393 418 328 49 399 18 1199 597
Cuts at: 4640 5427 5695 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1199 787 597 418 399 393 328
268 49 18
EcoO109I rG'GnC_Cy
Cuts at: 3100 3150 3100
Size: 50 5930
EcoRI G'AATT_C
Cuts at: 2272 3289 3667 2272
Size: 1017 378 4585
Fragments arranged by size:
4585 1017 378
EcoRV GAT'ATC
Cuts at: 2761 2761
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 5127 1678
Size: 3449 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 2546 2578 2745
Size: 32 135 32 529 32 413 32 167
Cuts at: 2745 2777 2820 2852 4621 4653 4695 4727 1373
Size: 32 43 32 1769 32 42 32 2626
Fragments arranged by size:
2626 1769 529 413 167 135 43 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 3938 4297 4456 518
Size: 648 888 1884 359 159 2042
Fragments arranged by size:
2042 1884 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2261 3396 4073 5221 5466 1606
Size: 655 1135 677 1148 245 2120
Fragments arranged by size:
2120 1148 1135 677 655 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2550 513
Size: 552 939 546 3943
Fragments arranged by size:
3943 939 552 546
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2171 3338 4883 1132
Size: 97 942 1167 1545 2229
Fragments arranged by size:
2229 1545 1167 942 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2710 1108
Size: 1602 4378
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PmlI CAC'GTG
Cuts at: 3265 3265
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3150 3150
Size: 5980
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PstI C_TGCA'G
Cuts at: 830 3332 830
Size: 2502 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2261 2261
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SanDI GG'GwC_CC
Cuts at: 3150 3150
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 3328 4463 5144 5335 826
Size: 219 2283 1135 681 191 1471
Fragments arranged by size:
2283 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 4084 4985 5247 820
Size: 197 926 231 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 231 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 2550 2646 3419 513
Size: 552 939 546 96 773 3074
Fragments arranged by size:
3074 939 773 552 546 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 3173 4007 4024 4177 4362 4807
Size: 1255 1131 462 834 17 153 185 445
Cuts at: 4807 325
Size: 1498
Fragments arranged by size:
1498 1255 1131 834 462 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2053 2085 5940 5972 2053
Size: 32 3855 32 2061
Fragments arranged by size:
3855 2061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI