Restriction map for
pFN21AA1333
(Circular) (Six-base) MAPSORT of: pFN21AA1333.seq Check: 8586 from: 1 to: 6823
pFN21AA1333 6823 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4166 1937
Size: 2229 4594
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 4886 6270 6538 1632
Size: 680 2574 1384 268 1917
Fragments arranged by size:
2574 1917 1384 680 268
AfeI AGC'GCT
Cuts at: 2774 2774
Size: 6823
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6626
AflIII A'CryG_T
Cuts at: 1203 2905 4120 5722 6699 1203
Size: 1702 1215 1602 977 1327
Fragments arranged by size:
1702 1602 1327 1215 977
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 3535 5552 3535
Size: 2017 4806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3934 1184
Size: 2750 4073
AlwNI CAG_nnn'CTG
Cuts at: 3691 4566 6138 3691
Size: 875 1572 4376
Fragments arranged by size:
4376 1572 875
ApaLI G'TGCA_C
Cuts at: 4880 6036 4880
Size: 1156 5667
ApoI r'AATT_y
Cuts at: 1493 2154 2450 2622 3026 3236 3313 3717 4132
Size: 661 296 172 404 210 77 404 415
Cuts at: 4132 4358 4510 6619 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 661 415 404 404 296 226
210 172 152 77
AseI AT'TA_AT
Cuts at: 160 1032 3053 5377 160
Size: 872 2021 2324 1606
Fragments arranged by size:
2324 2021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 4148 1870
Size: 73 2205 4545
Fragments arranged by size:
4545 2205 73
BamHI G'GATC_C
Cuts at: 2990 4153 4520 2990
Size: 1163 367 5293
Fragments arranged by size:
5293 1163 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4144 5599 618
Size: 325 217 627 2357 1455 1842
Fragments arranged by size:
2357 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4142 1391
Size: 2751 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3900 928
Size: 971 2001 3851
Fragments arranged by size:
3851 2001 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4715 5925 4715
Size: 1210 5613
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6823
BfrBI ATG'CAT
Cuts at: 3137 3137
Size: 6823
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5434 136
Size: 107 122 71 631 605 3762 1525
Fragments arranged by size:
3762 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3004 6818 3004
Size: 3814 3009
BlpI GC'TnA_GC
Cuts at: 4240 4240
Size: 6823
Bme1580I G_kGCm'C
Cuts at: 1790 4884 6040 1790
Size: 3094 1156 2573
Fragments arranged by size:
3094 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4554 5512 442
Size: 416 53 83 399 24 3137 958 1753
Fragments arranged by size:
3137 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3918 5483 1239
Size: 2679 1565 2579
Fragments arranged by size:
2679 2579 1565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3284 3303 3587 4948 5813 6111 3284
Size: 19 284 1361 865 298 3996
Fragments arranged by size:
3996 1361 865 298 284 19
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5486 882
Size: 617 3987 2219
Fragments arranged by size:
3987 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4614 493
Size: 711 3410 2702
Fragments arranged by size:
3410 2702 711
BsaBI GATnn'nnATC
Cuts at: 3497 6817 3497
Size: 3320 3503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4630 5012 5647 275
Size: 53 83 186 936 3097 382 635 1451
Fragments arranged by size:
3097 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5256 5654 5928 6075 6486 5256
Size: 398 274 147 411 5593
Fragments arranged by size:
5593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6598
Fragments arranged by size:
6598 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2806 1186
Size: 1620 5203
BseYI C'CCAG_C
Cuts at: 3450 6026 6522 3450
Size: 2576 496 3751
Fragments arranged by size:
3751 2576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4273 5034 6062 1540
Size: 275 186 2272 761 1028 2301
Fragments arranged by size:
2301 2272 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4884 4969 6040 4884
Size: 85 1071 5667
Fragments arranged by size:
5667 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3557 4334 4427 3137
Size: 420 777 93 5533
Fragments arranged by size:
5533 777 420 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4142 4884 4969 6040 1391
Size: 399 2352 742 85 1071 2174
Fragments arranged by size:
2352 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6486 6486
Size: 6823
BspHI T'CATG_A
Cuts at: 4712 6442 4712
Size: 1730 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4178 844
Size: 3334 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4710 1394
Size: 429 2887 3507
Fragments arranged by size:
3507 2887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5318 5492 65
Size: 1183 4070 174 1396
Fragments arranged by size:
4070 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5467 5654 1700
Size: 110 3657 187 2869
Fragments arranged by size:
3657 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2122 2570 96
Size: 2026 448 4349
Fragments arranged by size:
4349 2026 448
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 4883 5895 4883
Size: 1012 5811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2442 2546 794
Size: 1648 104 5071
Fragments arranged by size:
5071 1648 104
BstBI TT'CG_AA
Cuts at: 2710 5627 2710
Size: 2917 3906
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6823
BstXI CCAn_nnnn'nTGG
Cuts at: 2354 2446 2354
Size: 92 6731
BstYI r'GATC_y
Cuts at: 1972 2990 3004 4153 4520 4905 4922 6374 6460
Size: 1018 14 1149 367 385 17 1452 86
Cuts at: 6460 6472 6818 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1149 1018 385 367 346 86
17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6084
Fragments arranged by size:
6084 683 56
BtsI GCAGTG_nn'
Cuts at: 4339 5133 5153 4339
Size: 794 20 6009
Fragments arranged by size:
6009 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6788
DraI TTT'AAA
Cuts at: 3946 4127 3946
Size: 181 6642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5830 809
Size: 507 4514 1802
Fragments arranged by size:
4514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4270 5159 6788
Size: 54 1425 50 275 18 2440 889 1629
Cuts at: 6788 8
Size: 43
Fragments arranged by size:
2440 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4270 1537
Size: 275 2458 4090
Fragments arranged by size:
4090 2458 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2949 4753 1353
Size: 1596 1804 3423
Fragments arranged by size:
3423 1804 1596
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5410 5784 5930 387
Size: 1064 138 3821 374 146 1280
Fragments arranged by size:
3821 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 3918 4886 5483 6270 6538 1239
Size: 393 680 1606 968 597 787 268 1524
Fragments arranged by size:
1606 1524 968 787 680 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3288 3288
Size: 6823
EcoRI G'AATT_C
Cuts at: 2622 4132 4510 2622
Size: 1510 378 4935
Fragments arranged by size:
4935 1510 378
EcoRV GAT'ATC
Cuts at: 2209 2209
Size: 6823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3353 3650 3917 3353
Size: 297 267 6259
Fragments arranged by size:
6259 297 267
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 2776 5970 1678
Size: 1098 3194 2531
Fragments arranged by size:
3194 2531 1098
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2989 3021 3084 3116 3257
Size: 32 135 32 1417 32 63 32 141
Cuts at: 3257 3289 3521 3553 3860 3892 5464 5496 5538
Size: 32 232 32 307 32 1572 32 42
Cuts at: 5538 5570 1373
Size: 32 2626
Fragments arranged by size:
2626 1572 1417 307 232 141 135 63
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4167 4413 669
Size: 988 281 2229 246 3079
Fragments arranged by size:
3079 2229 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3828 748
Size: 3080 3743
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 2274 3171 3960 4422 2274
Size: 897 789 462 4675
Fragments arranged by size:
4675 897 789 462
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2931 3970 5937 6121 1305
Size: 1626 1039 1967 184 2007
Fragments arranged by size:
2007 1967 1626 1039 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5001
Fragments arranged by size:
5001 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2293 4781 5140 5299 518
Size: 648 1127 2488 359 159 2042
Fragments arranged by size:
2488 2042 1127 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3938 4239 4916 6064 6309 1606
Size: 2332 301 677 1148 245 2120
Fragments arranged by size:
2332 2120 1148 677 301 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6271
NdeI CA'TA_TG
Cuts at: 387 3166 3184 387
Size: 2779 18 4026
Fragments arranged by size:
4026 2779 18
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6823
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NsiI A_TGCA'T
Cuts at: 3139 3139
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 2909 3977 4124 4181 5726 1132
Size: 97 1680 1068 147 57 1545 2229
Fragments arranged by size:
2229 1680 1545 1068 147 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6823
PciI A'CATG_T
Cuts at: 2905 4120 5722 2905
Size: 1215 1602 4006
Fragments arranged by size:
4006 1602 1215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3117 1108
Size: 2009 4814
PfoI T'CCnGG_A
Cuts at: 3220 3220
Size: 6823
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3288 3288
Size: 6823
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 2475 3497 3593 4393 2475
Size: 1022 96 800 4905
Fragments arranged by size:
4905 1022 800 96
PstI C_TGCA'G
Cuts at: 830 2688 4175 830
Size: 1858 1487 3478
Fragments arranged by size:
3478 1858 1487
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
PvuII CAG'CTG
Cuts at: 3938 3938
Size: 6823
SalI G'TCGA_C
Cuts at: 1936 4165 1936
Size: 2229 4594
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 5071 6783 5071
Size: 1712 5111
SexAI A'CCwGG_T
Cuts at: 2020 3394 2020
Size: 1374 5449
SfcI C'TryA_G
Cuts at: 826 1045 2684 3522 4171 5306 5987 6178 826
Size: 219 1639 838 649 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 838 681 649 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 4150 1872
Size: 2278 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3263 3318 3566 4927 5828 6090
Size: 197 926 1320 55 248 1361 901 262
Cuts at: 6090 820
Size: 1553
Fragments arranged by size:
1553 1361 1320 926 901 262 248 197
55
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 4747 5
Size: 47 4695 2081
Fragments arranged by size:
4695 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6823
StyI C'CwwG_G
Cuts at: 513 1065 3700 4262 513
Size: 552 2635 562 3074
Fragments arranged by size:
3074 2635 562 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4850 4867 5020 5205 5650 325
Size: 1255 3270 17 153 185 445 1498
Fragments arranged by size:
3270 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2122 2570 3805
Size: 274 80 33 51 1443 145 448 1235
Cuts at: 3805 5069 6781 96
Size: 1264 1712 138
Fragments arranged by size:
1712 1443 1264 1235 448 274 145 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6783 6815 6783
Size: 32 6791
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6823
XbaI T'CTAG_A
Cuts at: 4159 4159
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 4148 1870
Size: 2278 4545
XmnI GAAnn'nnTTC
Cuts at: 3897 4952 3897
Size: 1055 5768
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI