Restriction map for
pFN21AA1331
(Circular) (Six-base) MAPSORT of: pFN21AA1331.seq Check: 5853 from: 1 to: 6472
pFN21AA1331 6472 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4282 278
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2271 2595 3515 4535 5919 6187 1632
Size: 473 166 324 920 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1020 920 473 324 268 166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 2055 5303 2055
Size: 3248 3224
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2456 1184
Size: 1272 5200
AlwNI CAG_nnn'CTG
Cuts at: 2333 3516 3769 4215 5787 2333
Size: 1183 253 446 1572 3018
Fragments arranged by size:
3018 1572 1183 446 253
ApaI G_GGCC'C
Cuts at: 2080 2080
Size: 6472
ApaLI G'TGCA_C
Cuts at: 4529 5685 4529
Size: 1156 5316
ApoI r'AATT_y
Cuts at: 1493 3781 4007 4159 6268 1493
Size: 2288 226 152 2109 1697
Fragments arranged by size:
2288 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3252 5026 160
Size: 872 2220 1774 1606
Fragments arranged by size:
2220 1774 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2418 3198 3797 1870
Size: 73 130 345 780 599 4545
Fragments arranged by size:
4545 780 599 345 130 73
AvrII C'CTAG_G
Cuts at: 3142 3142
Size: 6472
BamHI G'GATC_C
Cuts at: 2336 3802 4169 2336
Size: 1466 367 4639
Fragments arranged by size:
4639 1466 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2062 3415 3793 5248
Size: 325 217 627 246 29 1353 378 1455
Cuts at: 5248 618
Size: 1842
Fragments arranged by size:
1842 1455 1353 627 378 325 246 217
29
BanII G_rGCy'C
Cuts at: 1391 2080 3791 1391
Size: 689 1711 4072
Fragments arranged by size:
4072 1711 689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2491 928
Size: 971 592 4909
Fragments arranged by size:
4909 971 592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2939 4364 5574 2939
Size: 1425 1210 3837
Fragments arranged by size:
3837 1425 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5083 136
Size: 107 122 71 631 605 3411 1525
Fragments arranged by size:
3411 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 3889 3889
Size: 6472
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2080 4533 5689 1790
Size: 277 13 2453 1156 2573
Fragments arranged by size:
2573 2453 1156 277 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 4203 5161
Size: 416 53 83 399 24 952 1834 958
Cuts at: 5161 442
Size: 1753
Fragments arranged by size:
1834 1753 958 952 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2975 5132 1239
Size: 1736 2157 2579
Fragments arranged by size:
2579 2157 1736
Bpu10I CC'TnA_GC
Cuts at: 3064 3064
Size: 6472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4597 5462 5760 4597
Size: 865 298 5309
Fragments arranged by size:
5309 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5135 882
Size: 617 3636 2219
Fragments arranged by size:
3636 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 3576 6466 3576
Size: 2890 3582
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2599 4279 4661 5296
Size: 53 83 186 936 1066 1680 382 635
Cuts at: 5296 275
Size: 1451
Fragments arranged by size:
1680 1451 1066 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2055 3729 4905 5303 5577 5724 6135 2055
Size: 1674 1176 398 274 147 411 2392
Fragments arranged by size:
2392 1674 1176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3631 3661 1178
Size: 30 165 30 2228 30 3989
Fragments arranged by size:
3989 2228 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2633 3216 1186
Size: 1447 583 4442
Fragments arranged by size:
4442 1447 583
BseYI C'CCAG_C
Cuts at: 2766 5675 6171 2766
Size: 2909 496 3067
Fragments arranged by size:
3067 2909 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5899
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2056 2060 3922 4683 5711 1540
Size: 275 186 55 4 1862 761 1028 2301
Fragments arranged by size:
2301 1862 1028 761 275 186 55 4
BsiHKAI G_wGCw'C
Cuts at: 4533 4618 5689 4533
Size: 85 1071 5316
Fragments arranged by size:
5316 1071 85
BsmI GAATG_Cn'
Cuts at: 3983 4076 3983
Size: 93 6379
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2080 3791 4533 4618 5689 1391
Size: 399 277 13 1711 742 85 1071 2174
Fragments arranged by size:
2174 1711 1071 742 399 277 85 13
BspEI T'CCGG_A
Cuts at: 3729 6135 3729
Size: 2406 4066
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3827 844
Size: 2983 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3016 4967 5141 65
Size: 1183 1768 1951 174 1396
Fragments arranged by size:
1951 1768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 5116 5303 1700
Size: 110 245 3061 187 2869
Fragments arranged by size:
3061 2869 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 4561
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 3614 4532 5544 3614
Size: 918 1012 4542
Fragments arranged by size:
4542 1012 918
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3718 794
Size: 2924 3548
BstBI TT'CG_AA
Cuts at: 2303 5276 2303
Size: 2973 3499
BstEII G'GTnAC_C
Cuts at: 1184 3353 1184
Size: 2169 4303
BstYI r'GATC_y
Cuts at: 1972 2336 3513 3802 4169 4554 4571 6023 6109
Size: 364 1177 289 367 385 17 1452 86
Cuts at: 6109 6121 6467 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1177 385 367 364 346 289
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2544 2544
Size: 6472
BtgI C'CryG_G
Cuts at: 513 1065 2244 3148 513
Size: 552 1179 904 3837
Fragments arranged by size:
3837 1179 904 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5733
Fragments arranged by size:
5733 683 56
BtsI GCAGTG_nn'
Cuts at: 3007 3369 3988 4782 4802 3007
Size: 362 619 794 20 4677
Fragments arranged by size:
4677 794 619 362 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DraIII CAC_nnn'GTG
Cuts at: 2179 2179
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5479 809
Size: 507 4163 1802
Fragments arranged by size:
4163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2494 3919 4808
Size: 54 1425 50 275 18 664 1425 889
Cuts at: 4808 6437 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 664 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3919 1537
Size: 275 2107 4090
Fragments arranged by size:
4090 2107 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2314 2571 2769 3731 4402 1353
Size: 728 233 257 198 962 671 3423
Fragments arranged by size:
3423 962 728 671 257 233 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2981 5059 5433 5579 387
Size: 1064 138 1392 2078 374 146 1280
Fragments arranged by size:
2078 1392 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2271 2595 2975 3515 4535 5132
Size: 393 473 166 324 380 540 1020 597
Cuts at: 5132 5919 6187 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1020 787 597 540 473 393 380
324 268 166
EcoO109I rG'GnC_Cy
Cuts at: 2077 2843 2077
Size: 766 5706
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3195 3195
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 2098 5619 1678
Size: 420 3521 2531
Fragments arranged by size:
3521 2531 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5113 5145 5187 5219 1373
Size: 32 135 32 3541 32 42 32 2626
Fragments arranged by size:
3541 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3816 4062 669
Size: 988 281 1878 246 3079
Fragments arranged by size:
3079 1878 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2893 5586 5770 1305
Size: 1588 2693 184 2007
Fragments arranged by size:
2693 2007 1588 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4650
Fragments arranged by size:
4650 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4430 4789 4948 518
Size: 648 3264 359 159 2042
Fragments arranged by size:
3264 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2230 3631 3888 4565 5713 5958 1606
Size: 624 1401 257 677 1148 245 2120
Fragments arranged by size:
2120 1401 1148 677 624 257 245
NcoI C'CATG_G
Cuts at: 513 1065 2244 3148 513
Size: 552 1179 904 3837
Fragments arranged by size:
3837 1179 904 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 2353 3830 5375 1132
Size: 97 1124 1477 1545 2229
Fragments arranged by size:
2229 1545 1477 1124 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6472
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2766 1108
Size: 1658 4814
PfoI T'CCnGG_A
Cuts at: 2339 2339
Size: 6472
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2843 2843
Size: 6472
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PspOMI G'GGCC_C
Cuts at: 2076 2076
Size: 6472
PspXI vC'TCGA_Gb
Cuts at: 3198 3198
Size: 6472
PstI C_TGCA'G
Cuts at: 830 3824 830
Size: 2994 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2230 3631 2230
Size: 1401 5071
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SapI GCTCTTCn'nnn_
Cuts at: 2769 2769
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 3322 4720 6432 3322
Size: 1398 1712 3362
Fragments arranged by size:
3362 1712 1398
SexAI A'CCwGG_T
Cuts at: 2488 2488
Size: 6472
SfcI C'TryA_G
Cuts at: 826 1045 2118 3820 4955 5636 5827 826
Size: 219 1073 1702 1135 681 191 1471
Fragments arranged by size:
1702 1471 1135 1073 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 2075 3799 1872
Size: 203 1724 4545
Fragments arranged by size:
4545 1724 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3198 4576 5477 5739 820
Size: 197 926 1255 1378 901 262 1553
Fragments arranged by size:
1553 1378 1255 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SrfI GCCC'GGGC
Cuts at: 2075 2075
Size: 6472
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6472
StyI C'CwwG_G
Cuts at: 513 1065 2244 3142 3148 3911 513
Size: 552 1179 898 6 763 3074
Fragments arranged by size:
3074 1179 898 763 552 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4499 4516 4669 4854 5299 325
Size: 1255 2919 17 153 185 445 1498
Fragments arranged by size:
2919 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2609 3320
Size: 274 80 33 51 1443 30 602 711
Cuts at: 3320 4718 6430 96
Size: 1398 1712 138
Fragments arranged by size:
1712 1443 1398 711 602 274 138 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 3631 3663 6432 6464 2421
Size: 32 1178 32 2769 32 2429
Fragments arranged by size:
2769 2429 1178 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6472
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2242
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 3198 1943
Size: 1255 5217
XmaI C'CCGG_G
Cuts at: 1870 2073 3797 1870
Size: 203 1724 4545
Fragments arranged by size:
4545 1724 203
XmnI GAAnn'nnTTC
Cuts at: 3408 3753 4601 3408
Size: 345 848 5279
Fragments arranged by size:
5279 848 345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4280 276
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PsrI RsrII SacI SacII SanDI SfiI
SfoI SwaI