Restriction map for
pFN21AA1321
(Circular) (Six-base) MAPSORT of: pFN21AA1321.seq Check: 4061 from: 1 to: 6790
pFN21AA1321 6790 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4600 278
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2397 4133 1937
Size: 460 1736 4594
Fragments arranged by size:
4594 1736 460
Acc65I G'GTAC_C
Cuts at: 4111 4111
Size: 6790
AclI AA'CG_TT
Cuts at: 1563 4917 5290 1563
Size: 3354 373 3063
Fragments arranged by size:
3354 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 2961 3369 4853 6237 6505 1632
Size: 797 532 408 1484 1384 268 1917
Fragments arranged by size:
1917 1484 1384 797 532 408 268
AfeI AGC'GCT
Cuts at: 3219 3219
Size: 6790
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6593
AflIII A'CryG_T
Cuts at: 1203 5689 6666 1203
Size: 4486 977 1327
Fragments arranged by size:
4486 1327 977
AgeI A'CCGG_T
Cuts at: 5621 5621
Size: 6790
AhdI GACnn_n'nnGTC
Cuts at: 5519 5519
Size: 6790
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6790
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3661 3693 3661
Size: 32 6758
AlwNI CAG_nnn'CTG
Cuts at: 3272 3307 3392 3671 4533 6105 3272
Size: 35 85 279 862 1572 3957
Fragments arranged by size:
3957 1572 862 279 85 35
ApaLI G'TGCA_C
Cuts at: 4847 6003 4847
Size: 1156 5634
ApoI r'AATT_y
Cuts at: 1493 2461 4024 4099 4325 4477 6586 1493
Size: 968 1563 75 226 152 2109 1697
Fragments arranged by size:
2109 1697 1563 968 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 5344 160
Size: 872 4312 1606
Fragments arranged by size:
4312 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
AvaI C'yCGr_G
Cuts at: 1870 1943 4115 1870
Size: 73 2172 4545
Fragments arranged by size:
4545 2172 73
BamHI G'GATC_C
Cuts at: 4120 4487 4120
Size: 367 6423
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4111 5566 618
Size: 325 217 627 2324 1455 1842
Fragments arranged by size:
2324 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3549 4109 1391
Size: 2158 560 4072
Fragments arranged by size:
4072 2158 560
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5819
BbvCI CC'TCA_GC
Cuts at: 2034 2073 2034
Size: 39 6751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4981 5015 1903
Size: 34 3044 34 3678
Fragments arranged by size:
3678 3044 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4682 5892 4682
Size: 1210 5580
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6790
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5401 136
Size: 107 122 71 631 605 3729 1525
Fragments arranged by size:
3729 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6785 6785
Size: 6790
BlpI GC'TnA_GC
Cuts at: 4207 4207
Size: 6790
Bme1580I G_kGCm'C
Cuts at: 1790 4851 6007 1790
Size: 3061 1156 2573
Fragments arranged by size:
3061 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6790
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2865 3020 3543
Size: 416 53 83 399 24 1448 155 523
Cuts at: 3543 3582 3783 4521 5479 442
Size: 39 201 738 958 1753
Fragments arranged by size:
1753 1448 958 738 523 416 399 201
155 83 53 39 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2328 2541 2883 3276 3350 3725 5450
Size: 992 97 213 342 393 74 375 1725
Cuts at: 5450 1239
Size: 2579
Fragments arranged by size:
2579 1725 992 393 375 342 213 97
74
Bpu10I CC'TnA_GC
Cuts at: 2034 2073 2034
Size: 39 6751
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3708 4915 5780 6078 3708
Size: 1207 865 298 4420
Fragments arranged by size:
4420 1207 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2741 5453 882
Size: 617 1242 2712 2219
Fragments arranged by size:
2712 2219 1242 617
BsaAI yAC'GTr
Cuts at: 493 1204 4581 493
Size: 711 3377 2702
Fragments arranged by size:
3377 2702 711
BsaBI GATnn'nnATC
Cuts at: 6784 6784
Size: 6790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4597 4979 5614 275
Size: 53 83 186 936 3064 382 635 1451
Fragments arranged by size:
3064 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5223 5621 5895 6042 6453 5223
Size: 398 274 147 411 5560
Fragments arranged by size:
5560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2229 2259 1178
Size: 30 165 30 826 30 5709
Fragments arranged by size:
5709 826 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 1186
Size: 838 5952
BseYI C'CCAG_C
Cuts at: 2208 3960 5993 6489 2208
Size: 1752 2033 496 2509
Fragments arranged by size:
2509 2033 1752 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2744 1163
Size: 573 1008 5209
Fragments arranged by size:
5209 1008 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4240 5001 6029 1540
Size: 275 186 2239 761 1028 2301
Fragments arranged by size:
2301 2239 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3458 4851 4936 6007 3458
Size: 1393 85 1071 4241
Fragments arranged by size:
4241 1393 1071 85
BsmI GAATG_Cn'
Cuts at: 3738 4301 4394 3738
Size: 563 93 6134
Fragments arranged by size:
6134 563 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3458 3549 4109 4851 4936 6007 1391
Size: 399 1668 91 560 742 85 1071 2174
Fragments arranged by size:
2174 1668 1071 742 560 399 91 85
BspEI T'CCGG_A
Cuts at: 6453 6453
Size: 6790
BspHI T'CATG_A
Cuts at: 4679 6409 4679
Size: 1730 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 2893 3813 4145 844
Size: 1340 709 920 332 3489
Fragments arranged by size:
3489 1340 920 709 332
BsrBI CCG'CTC
Cuts at: 1394 1823 2652 4677 1394
Size: 429 829 2025 3507
Fragments arranged by size:
3507 2025 829 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5285 5459 65
Size: 1183 4037 174 1396
Fragments arranged by size:
4037 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5434 5621 1700
Size: 110 3624 187 2869
Fragments arranged by size:
3624 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6790
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6790
BssSI C'ACGA_G
Cuts at: 3001 4850 5862 3001
Size: 1849 1012 3929
Fragments arranged by size:
3929 1849 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3445 794
Size: 2651 4139
BstBI TT'CG_AA
Cuts at: 2831 5594 2831
Size: 2763 4027
BstEII G'GTnAC_C
Cuts at: 1184 3800 1184
Size: 2616 4174
BstYI r'GATC_y
Cuts at: 1972 2642 3469 4039 4120 4487 4872 4889 6341
Size: 670 827 570 81 367 385 17 1452
Cuts at: 6341 6427 6439 6785 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 827 670 570 385 367 346
86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 3664 3977 3664
Size: 313 6477
BtgI C'CryG_G
Cuts at: 513 1065 2004 4071 513
Size: 552 939 2067 3232
Fragments arranged by size:
3232 2067 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6051
Fragments arranged by size:
6051 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3305 4306 5100 5120 3055
Size: 250 1001 794 20 4725
Fragments arranged by size:
4725 1001 794 250 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2553 2588 529
Size: 35 1989 35 4731
Fragments arranged by size:
4731 1989 35 35
DraI TTT'AAA
Cuts at: 2328 3016 4094 2328
Size: 688 1078 5024
Fragments arranged by size:
5024 1078 688
DraIII CAC_nnn'GTG
Cuts at: 3647 3647
Size: 6790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5797 809
Size: 507 4481 1802
Fragments arranged by size:
4481 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2020 4237 5126
Size: 54 1425 50 275 18 190 2217 889
Cuts at: 5126 6755 8
Size: 1629 43
Fragments arranged by size:
2217 1629 1425 889 275 190 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4237 1537
Size: 275 2425 4090
Fragments arranged by size:
4090 2425 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3127 3349 4720 1353
Size: 1774 222 1371 3423
Fragments arranged by size:
3423 1774 1371 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5377 5751 5897 387
Size: 1064 138 3788 374 146 1280
Fragments arranged by size:
3788 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2328 2429 2541 2883 2961 3276
Size: 393 599 97 101 112 342 78 315
Cuts at: 3276 3350 3369 3725 4853 5450 6237 6505 1239
Size: 74 19 356 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 787 599 597 393 356 342
315 268 112 101 97 78 74 19
EcoO109I rG'GnC_Cy
Cuts at: 2371 3205 2371
Size: 834 5956
EcoRI G'AATT_C
Cuts at: 4099 4477 4099
Size: 378 6412
EcoRV GAT'ATC
Cuts at: 3508 3508
Size: 6790
FspI TGC'GCA
Cuts at: 5296 5296
Size: 6790
HaeII r_GCGC'y
Cuts at: 1678 3221 5937 1678
Size: 1543 2716 2531
Fragments arranged by size:
2716 2531 1543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 3461 3493 3706
Size: 32 135 32 944 32 913 32 213
Cuts at: 3706 3738 3856 3888 5431 5463 5505 5537 1373
Size: 32 118 32 1543 32 42 32 2626
Fragments arranged by size:
2626 1543 944 913 213 135 118 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4134 4380 669
Size: 988 281 2196 246 3079
Fragments arranged by size:
3079 2196 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6790
HpaI GTT'AAC
Cuts at: 4380 4380
Size: 6790
KpnI G_GTAC'C
Cuts at: 4115 4115
Size: 6790
MfeI C'AATT_G
Cuts at: 4389 4389
Size: 6790
MluI A'CGCG_T
Cuts at: 6666 6666
Size: 6790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5904 6088 1305
Size: 4599 184 2007
Fragments arranged by size:
4599 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4968
Fragments arranged by size:
4968 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3798 4748 5107 5266 518
Size: 648 2632 950 359 159 2042
Fragments arranged by size:
2632 2042 950 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 2981 3392 4206 4883 6031 6276 1606
Size: 615 760 411 814 677 1148 245 2120
Fragments arranged by size:
2120 1148 814 760 677 615 411 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4071 513
Size: 552 939 2067 3232
Fragments arranged by size:
3232 2067 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6790
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6790
NotI GC'GGCC_GC
Cuts at: 4237 4237
Size: 6790
NspI r_CATG'y
Cuts at: 1132 1229 2975 4148 5693 1132
Size: 97 1746 1173 1545 2229
Fragments arranged by size:
2229 1746 1545 1173 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6790
PciI A'CATG_T
Cuts at: 5689 5689
Size: 6790
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6790
PfoI T'CCnGG_A
Cuts at: 3366 3366
Size: 6790
PmeI GTTT'AAAC
Cuts at: 4094 4094
Size: 6790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4859 4891 6372 6404 4859
Size: 32 1481 32 5245
Fragments arranged by size:
5245 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 3205 2371
Size: 834 5956
PshAI GACnn'nnGTC
Cuts at: 1613 2753 3375 1613
Size: 1140 622 5028
Fragments arranged by size:
5028 1140 622
PsiI TTA'TAA
Cuts at: 3539 4360 3539
Size: 821 5969
PstI C_TGCA'G
Cuts at: 830 2181 3810 4142 830
Size: 1351 1629 332 3478
Fragments arranged by size:
3478 1629 1351 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6790
PvuII CAG'CTG
Cuts at: 2981 3392 2981
Size: 411 6379
SalI G'TCGA_C
Cuts at: 1936 4132 1936
Size: 2196 4594
SbfI CC_TGCA'GG
Cuts at: 4142 4142
Size: 6790
ScaI AGT'ACT
Cuts at: 5038 6750 5038
Size: 1712 5078
SexAI A'CCwGG_T
Cuts at: 2926 2926
Size: 6790
SfcI C'TryA_G
Cuts at: 826 1045 2177 2535 2797 3806 4138 5273 5954
Size: 219 1132 358 262 1009 332 1135 681
Cuts at: 5954 6145 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1132 1009 681 358 332 262
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6790
SmaI CCC'GGG
Cuts at: 1872 4117 1872
Size: 2245 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3723 4894 5795 6057 820
Size: 197 926 1780 1171 901 262 1553
Fragments arranged by size:
1780 1553 1171 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4581 493
Size: 4088 2702
SpeI A'CTAG_T
Cuts at: 152 3716 3758 152
Size: 3564 42 3184
Fragments arranged by size:
3564 3184 42
SphI G_CATG'C
Cuts at: 1132 2975 4148 1132
Size: 1843 1173 3774
Fragments arranged by size:
3774 1843 1173
SspI AAT'ATT
Cuts at: 5 52 4714 5
Size: 47 4662 2081
Fragments arranged by size:
4662 2081 47
StuI AGG'CCT
Cuts at: 1517 3942 1517
Size: 2425 4365
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 2697 3690 3780 4071 4229
Size: 552 939 198 495 993 90 291 158
Cuts at: 4229 513
Size: 3074
Fragments arranged by size:
3074 993 939 552 495 291 198 158
90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2882 4817 4834 4987 5172 5617 325
Size: 1255 1302 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1302 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5036 6748 96
Size: 274 80 33 51 1443 3059 1712 138
Fragments arranged by size:
3059 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3745 3777 6750 6782 3745
Size: 32 2973 32 3753
Fragments arranged by size:
3753 2973 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6790
XbaI T'CTAG_A
Cuts at: 4126 4126
Size: 6790
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6790
XmaI C'CCGG_G
Cuts at: 1870 4115 1870
Size: 2245 4545
XmnI GAAnn'nnTTC
Cuts at: 3657 4919 3657
Size: 1262 5528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4598 276
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI