Restriction map for
pFN21AA1313
(Circular) (Six-base) MAPSORT of: pFN21AA1313.seq Check: 5705 from: 1 to: 8008
pFN21AA1313 8008 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3481 3481
Size: 8008
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3672 5818 278
Size: 53 83 186 936 2136 2146 2468
Fragments arranged by size:
2468 2146 2136 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 3729 5351 1937
Size: 889 903 1622 4594
Fragments arranged by size:
4594 1622 903 889
Acc65I G'GTAC_C
Cuts at: 4228 5329 4228
Size: 1101 6907
AclI AA'CG_TT
Cuts at: 1563 4263 6135 6508 1563
Size: 2700 1872 373 3063
Fragments arranged by size:
3063 2700 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 4595 5211 5270 5312 6071 7455 7723
Size: 531 2432 616 59 42 759 1384 268
Cuts at: 7723 1632
Size: 1917
Fragments arranged by size:
2432 1917 1384 759 616 531 268 59
42
AfeI AGC'GCT
Cuts at: 2461 2461
Size: 8008
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7811
AflIII A'CryG_T
Cuts at: 1203 2934 3151 6907 7884 1203
Size: 1731 217 3756 977 1327
Fragments arranged by size:
3756 1731 1327 977 217
AgeI A'CCGG_T
Cuts at: 6839 6839
Size: 8008
AhdI GACnn_n'nnGTC
Cuts at: 6737 6737
Size: 8008
AleI CACnn'nnGTG
Cuts at: 1166 4676 1166
Size: 3510 4498
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4126 1184
Size: 2942 5066
AlwNI CAG_nnn'CTG
Cuts at: 4324 4773 5751 7323 4324
Size: 449 978 1572 5009
Fragments arranged by size:
5009 1572 978 449
ApaI G_GGCC'C
Cuts at: 2973 4437 2973
Size: 1464 6544
ApaLI G'TGCA_C
Cuts at: 6065 7221 6065
Size: 1156 6852
ApoI r'AATT_y
Cuts at: 1493 3593 5317 5543 5695 7804 1493
Size: 2100 1724 226 152 2109 1697
Fragments arranged by size:
2109 2100 1724 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2073 6562 160
Size: 872 1041 4489 1606
Fragments arranged by size:
4489 1606 1041 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 3094 4233 5333 1870
Size: 73 369 782 1139 1100 4545
Fragments arranged by size:
4545 1139 1100 782 369 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4638 4671 4638
Size: 33 7975
BamHI G'GATC_C
Cuts at: 5338 5705 5338
Size: 367 7641
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2663 2807 3410 4228 5329
Size: 325 217 627 876 144 603 818 1101
Cuts at: 5329 6784 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1101 876 818 627 603 325
217 144
BanII G_rGCy'C
Cuts at: 1391 2294 2508 2868 2973 3905 4025 4064 4437
Size: 903 214 360 105 932 120 39 373
Cuts at: 4437 4525 5084 5202 5327 1391
Size: 88 559 118 125 4072
Fragments arranged by size:
4072 932 903 559 373 360 214 125
120 118 105 88 39
BbeI G_GCGC'C
Cuts at: 3414 3414
Size: 8008
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3502 3789 928
Size: 971 1603 287 5147
Fragments arranged by size:
5147 1603 971 287
BbvCI CC'TCA_GC
Cuts at: 4944 4944
Size: 8008
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6199 6233 1903
Size: 34 4262 34 3678
Fragments arranged by size:
4262 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5900 7110 5900
Size: 1210 6798
BclI T'GATC_A
Cuts at: 1548 4705 4711 1548
Size: 3157 6 4845
Fragments arranged by size:
4845 3157 6
BfrBI ATG'CAT
Cuts at: 5230 5230
Size: 8008
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2129 6619 136
Size: 107 122 71 631 605 457 4490 1525
Fragments arranged by size:
4490 1525 631 605 457 122 107 71
BglII A'GATC_T
Cuts at: 2415 8003 2415
Size: 5588 2420
BlpI GC'TnA_GC
Cuts at: 5196 5425 5196
Size: 229 7779
Bme1580I G_kGCm'C
Cuts at: 1790 2666 2810 2934 2973 3183 3297 4437 6069
Size: 876 144 124 39 210 114 1140 1632
Cuts at: 6069 7225 1790
Size: 1156 2573
Fragments arranged by size:
2573 1632 1156 1140 876 210 144 124
114 39
BmgBI CAC'GTC
Cuts at: 1318 2950 3152 1318
Size: 1632 202 6174
Fragments arranged by size:
6174 1632 202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2379 5739 6697
Size: 416 53 83 399 24 962 3360 958
Cuts at: 6697 442
Size: 1753
Fragments arranged by size:
3360 1753 962 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8008
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 8008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 2552 5165 6668 1239
Size: 1193 120 2613 1503 2579
Fragments arranged by size:
2613 2579 1503 1193 120
Bpu10I CC'TnA_GC
Cuts at: 2958 4815 4944 2958
Size: 1857 129 6022
Fragments arranged by size:
6022 1857 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 5060 6133 6998 7296 2063
Size: 2997 1073 865 298 2775
Fragments arranged by size:
2997 2775 1073 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3458 6671 882
Size: 617 1959 3213 2219
Fragments arranged by size:
3213 2219 1959 617
BsaAI yAC'GTr
Cuts at: 493 1204 2935 3103 4510 5799 493
Size: 711 1731 168 1407 1289 2702
Fragments arranged by size:
2702 1731 1407 1289 711 168
BsaBI GATnn'nnATC
Cuts at: 4710 8002 4710
Size: 3292 4716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3411 3669 5815 6197
Size: 53 83 186 936 1878 258 2146 382
Cuts at: 6197 6832 275
Size: 635 1451
Fragments arranged by size:
2146 1878 1451 936 635 382 258 186
83 53
BsaWI w'CCGG_w
Cuts at: 4134 6441 6839 7113 7260 7671 4134
Size: 2307 398 274 147 411 4471
Fragments arranged by size:
4471 2307 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7783
Fragments arranged by size:
7783 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 4259 4279 4607 4961 1186
Size: 925 2148 20 328 354 4233
Fragments arranged by size:
4233 2148 925 354 328 20
BseYI C'CCAG_C
Cuts at: 2231 3169 4437 5131 7211 7707 2231
Size: 938 1268 694 2080 496 2532
Fragments arranged by size:
2532 2080 1268 938 694 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 2972 3176 3410 1163
Size: 573 777 459 204 234 5761
Fragments arranged by size:
5761 777 573 459 234 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3838 5458 6219 7247 1540
Size: 275 186 853 984 1620 761 1028 2301
Fragments arranged by size:
2301 1620 1028 984 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2868 3330 3905 4355 5084 6069 6154 7225
Size: 360 462 575 450 729 985 85 1071
Cuts at: 7225 2508
Size: 3291
Fragments arranged by size:
3291 1071 985 729 575 462 450 360
85
BsmI GAATG_Cn'
Cuts at: 4748 5519 5612 4748
Size: 771 93 7144
Fragments arranged by size:
7144 771 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2594 2594
Size: 8008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 2508 2666 2810 2868 2934 2973
Size: 399 504 214 158 144 58 66 39
Cuts at: 2973 3183 3297 3330 3905 4025 4064 4355 4437
Size: 210 114 33 575 120 39 291 82
Cuts at: 4437 4525 5084 5202 5327 6069 6154 7225 1391
Size: 88 559 118 125 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 559 504 399 291
214 210 158 144 125 120 118 114
88 85 82 66 58 39 39 33
BspEI T'CCGG_A
Cuts at: 4134 7671 4134
Size: 3537 4471
BspHI T'CATG_A
Cuts at: 4708 5897 7627 4708
Size: 1189 1730 5089
Fragments arranged by size:
5089 1730 1189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3054 3481 5363 844
Size: 2210 427 1882 3489
Fragments arranged by size:
3489 2210 1882 427
BsrBI CCG'CTC
Cuts at: 1394 1823 4344 5895 1394
Size: 429 2521 1551 3507
Fragments arranged by size:
3507 2521 1551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3181 4065 4729 5038 5115 6503 6677
Size: 1183 1933 884 664 309 77 1388 174
Cuts at: 6677 65
Size: 1396
Fragments arranged by size:
1933 1396 1388 1183 884 664 309 174
77
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 3034 6652 6839 1700
Size: 110 213 1011 3618 187 2869
Fragments arranged by size:
3618 2869 1011 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8008
BssHII G'CGCG_C
Cuts at: 1925 2104 1925
Size: 179 7829
BssSI C'ACGA_G
Cuts at: 2573 3572 6068 7080 2573
Size: 999 2496 1012 3501
Fragments arranged by size:
3501 2496 1012 999
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4324 794
Size: 3530 4478
BstBI TT'CG_AA
Cuts at: 3591 6812 3591
Size: 3221 4787
BstEII G'GTnAC_C
Cuts at: 1184 2248 2686 2998 1184
Size: 1064 438 312 6194
Fragments arranged by size:
6194 1064 438 312
BstXI CCAn_nnnn'nTGG
Cuts at: 2332 3220 3866 5122 2332
Size: 888 646 1256 5218
Fragments arranged by size:
5218 1256 888 646
BstYI r'GATC_y
Cuts at: 1972 2415 4131 4691 5338 5705 6090 6107 7559
Size: 443 1716 560 647 367 385 17 1452
Cuts at: 7559 7645 7657 8003 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1716 1452 647 560 443 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3838 3943 4686 5203 513
Size: 552 939 1834 105 743 517 3318
Fragments arranged by size:
3318 1834 939 743 552 517 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4819 487
Size: 683 56 3593 3676
Fragments arranged by size:
3676 3593 683 56
BtsI GCAGTG_nn'
Cuts at: 2628 2989 3164 3469 3629 3717 5524 6318 6338
Size: 361 175 305 160 88 1807 794 20
Cuts at: 6338 2628
Size: 4298
Fragments arranged by size:
4298 1807 794 361 305 175 160 88
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7973
DraI TTT'AAA
Cuts at: 5312 5312
Size: 8008
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 8008
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3506 7015 809
Size: 507 2190 3509 1802
Fragments arranged by size:
3509 2190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2130 2815
Size: 54 1425 50 275 18 210 90 685
Cuts at: 2815 3606 3805 4756 5455 6344 7973 8
Size: 791 199 951 699 889 1629 43
Fragments arranged by size:
1629 1425 951 889 791 699 685 275
210 199 90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5455 1537
Size: 275 3643 4090
Fragments arranged by size:
4090 3643 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2919 4282 5938 1353
Size: 735 831 1363 1656 3423
Fragments arranged by size:
3423 1656 1363 831 735
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2768 3021 4211 6595 6969 7115
Size: 1064 138 1179 253 1190 2384 374 146
Cuts at: 7115 387
Size: 1280
Fragments arranged by size:
2384 1280 1190 1179 1064 374 253 146
138
EcoICRI GAG'CTC
Cuts at: 2506 2866 3903 5082 2506
Size: 360 1037 1179 5432
Fragments arranged by size:
5432 1179 1037 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2432 2552 4595 5165 5211 5270
Size: 393 531 269 120 2043 570 46 59
Cuts at: 5270 5312 6071 6668 7455 7723 1239
Size: 42 759 597 787 268 1524
Fragments arranged by size:
2043 1524 787 759 597 570 531 393
269 268 120 59 46 42
EcoO109I rG'GnC_Cy
Cuts at: 2365 2969 4433 4434 2365
Size: 604 1464 1 5939
Fragments arranged by size:
5939 1464 604 1
EcoRI G'AATT_C
Cuts at: 3593 5317 5695 3593
Size: 1724 378 5906
Fragments arranged by size:
5906 1724 378
EcoRV GAT'ATC
Cuts at: 5299 5299
Size: 8008
FspI TGC'GCA
Cuts at: 6514 6514
Size: 8008
HaeII r_GCGC'y
Cuts at: 1678 2463 2913 3090 3414 7155 1678
Size: 785 450 177 324 3741 2531
Fragments arranged by size:
3741 2531 785 450 324 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 2459 2491 2591
Size: 32 135 32 734 32 121 32 100
Cuts at: 2591 2623 3119 3151 4690 4722 6649 6681 6723
Size: 32 496 32 1539 32 1927 32 42
Cuts at: 6723 6755 1373
Size: 32 2626
Fragments arranged by size:
2626 1927 1539 734 496 135 121 100
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2848 3058 3673 4657 5008 5158
Size: 988 281 910 210 615 984 351 150
Cuts at: 5158 5167 5352 5598 669
Size: 9 185 246 3079
Fragments arranged by size:
3079 988 984 910 615 351 281 246
210 185 150 9
HindIII A'AGCT_T
Cuts at: 748 2482 748
Size: 1734 6274
HpaI GTT'AAC
Cuts at: 5598 5598
Size: 8008
KasI G'GCGC_C
Cuts at: 3410 3410
Size: 8008
KpnI G_GTAC'C
Cuts at: 4232 5333 4232
Size: 1101 6907
MfeI C'AATT_G
Cuts at: 5607 5607
Size: 8008
MluI A'CGCG_T
Cuts at: 7884 7884
Size: 8008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 4527 7122 7306 1305
Size: 918 2304 2595 184 2007
Fragments arranged by size:
2595 2304 2007 918 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 2132 3608 4758 10
Size: 54 1425 343 210 90 1476 1150 3260
Fragments arranged by size:
3260 1476 1425 1150 343 210 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4676 5966 6325 6484 518
Size: 648 3510 1290 359 159 2042
Fragments arranged by size:
3510 2042 1290 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2452 3840 4630 5424 6101 7249 7494 1606
Size: 846 1388 790 794 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 846 794 790 677 245
NarI GG'CG_CC
Cuts at: 3411 3411
Size: 8008
NcoI C'CATG_G
Cuts at: 513 1065 2004 4686 513
Size: 552 939 2682 3835
Fragments arranged by size:
3835 2682 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8008
NotI GC'GGCC_GC
Cuts at: 5455 5455
Size: 8008
NruI TCG'CGA
Cuts at: 3132 3438 3132
Size: 306 7702
NsiI A_TGCA'T
Cuts at: 5232 5232
Size: 8008
NspI r_CATG'y
Cuts at: 1132 1229 3505 4823 5366 6911 1132
Size: 97 2276 1318 543 1545 2229
Fragments arranged by size:
2276 2229 1545 1318 543 97
PasI CC'CwG_GG
Cuts at: 1348 4057 4330 1348
Size: 2709 273 5026
Fragments arranged by size:
5026 2709 273
PciI A'CATG_T
Cuts at: 6907 6907
Size: 8008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2049 1108
Size: 941 7067
PfoI T'CCnGG_A
Cuts at: 5019 5019
Size: 8008
PmeI GTTT'AAAC
Cuts at: 5312 5312
Size: 8008
PmlI CAC'GTG
Cuts at: 2935 2935
Size: 8008
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6077 6109 7590 7622 6077
Size: 32 1481 32 6463
Fragments arranged by size:
6463 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2365
Size: 8008
PshAI GACnn'nnGTC
Cuts at: 1613 3450 3770 1613
Size: 1837 320 5851
Fragments arranged by size:
5851 1837 320
PsiI TTA'TAA
Cuts at: 5578 5578
Size: 8008
PspOMI G'GGCC_C
Cuts at: 2969 4433 2969
Size: 1464 6544
PspXI vC'TCGA_Gb
Cuts at: 2312 2312
Size: 8008
PstI C_TGCA'G
Cuts at: 830 3195 3306 4151 4997 5360 830
Size: 2365 111 845 846 363 3478
Fragments arranged by size:
3478 2365 846 845 363 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8008
SacI G_AGCT'C
Cuts at: 2508 2868 3905 5084 2508
Size: 360 1037 1179 5432
Fragments arranged by size:
5432 1179 1037 360
SacII CC_GC'GG
Cuts at: 3841 3841
Size: 8008
SalI G'TCGA_C
Cuts at: 1936 5350 1936
Size: 3414 4594
SapI GCTCTTCn'nnn_
Cuts at: 4282 4282
Size: 8008
SbfI CC_TGCA'GG
Cuts at: 5360 5360
Size: 8008
ScaI AGT'ACT
Cuts at: 2084 4163 6256 7968 2084
Size: 2079 2093 1712 2124
Fragments arranged by size:
2124 2093 2079 1712
SexAI A'CCwGG_T
Cuts at: 3378 3759 3858 3378
Size: 381 99 7528
Fragments arranged by size:
7528 381 99
SfcI C'TryA_G
Cuts at: 826 1045 2614 3191 3302 4147 4993 5356 6491
Size: 219 1569 577 111 845 846 363 1135
Cuts at: 6491 7172 7363 826
Size: 681 191 1471
Fragments arranged by size:
1569 1471 1135 846 845 681 577 363
219 191 111
SfoI GGC'GCC
Cuts at: 3412 3412
Size: 8008
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8008
SmaI CCC'GGG
Cuts at: 1872 3096 4235 5335 1872
Size: 1224 1139 1100 4545
Fragments arranged by size:
4545 1224 1139 1100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2312 5075 6112 7013 7275
Size: 197 926 135 234 2763 1037 901 262
Cuts at: 7275 820
Size: 1553
Fragments arranged by size:
2763 1553 1037 926 901 262 234 197
135
SnaBI TAC'GTA
Cuts at: 493 5799 493
Size: 5306 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8008
SphI G_CATG'C
Cuts at: 1132 5366 1132
Size: 4234 3774
SspI AAT'ATT
Cuts at: 5 52 5932 5
Size: 47 5880 2081
Fragments arranged by size:
5880 2081 47
StuI AGG'CCT
Cuts at: 1517 3141 4954 1517
Size: 1624 1813 4571
Fragments arranged by size:
4571 1813 1624
StyI C'CwwG_G
Cuts at: 513 1065 2004 3111 3582 3609 4686 5001 5447
Size: 552 939 1107 471 27 1077 315 446
Cuts at: 5447 513
Size: 3074
Fragments arranged by size:
3074 1107 1077 939 552 471 446 315
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 3842 6035 6052 6205 6390 6835
Size: 1255 1125 1137 2193 17 153 185 445
Cuts at: 6835 325
Size: 1498
Fragments arranged by size:
2193 1498 1255 1137 1125 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 4161 6254
Size: 274 80 33 51 1443 105 2079 2093
Cuts at: 6254 7966 96
Size: 1712 138
Fragments arranged by size:
2093 2079 1712 1443 274 138 105 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2760 2792 7968 8000 2760
Size: 32 5176 32 2768
Fragments arranged by size:
5176 2768 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2605 3860 1316
Size: 1289 1255 5464
Fragments arranged by size:
5464 1289 1255
XbaI T'CTAG_A
Cuts at: 5344 5344
Size: 8008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2818 3220 2818
Size: 402 7606
XhoI C'TCGA_G
Cuts at: 1943 2312 1943
Size: 369 7639
XmaI C'CCGG_G
Cuts at: 1870 3094 4233 5333 1870
Size: 1224 1139 1100 4545
Fragments arranged by size:
4545 1224 1139 1100
XmnI GAAnn'nnTTC
Cuts at: 4725 6137 4725
Size: 1412 6596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3670 5816 276
Size: 53 83 186 936 2136 2146 2468
Fragments arranged by size:
2468 2146 2136 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI BstZ17I Bsu36I EcoNI FalI
FseI FspAI NaeI NgoMIV PacI PsrI PvuII RsrII
SanDI SfiI SrfI SwaI