Restriction map for
pFN21AA1299
(Circular) (Six-base) MAPSORT of: pFN21AA1299.seq Check: 1639 from: 1 to: 6718
pFN21AA1299 6718 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3741 3741
Size: 6718
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2991 4528 278
Size: 53 83 186 936 1455 1537 2468
Fragments arranged by size:
2468 1537 1455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3256 4061 1937
Size: 1319 805 4594
Fragments arranged by size:
4594 1319 805
Acc65I G'GTAC_C
Cuts at: 4039 4039
Size: 6718
AclI AA'CG_TT
Cuts at: 1563 4845 5218 1563
Size: 3282 373 3063
Fragments arranged by size:
3282 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2823 4781 6165 6433 1632
Size: 1191 1958 1384 268 1917
Fragments arranged by size:
1958 1917 1384 1191 268
AfeI AGC'GCT
Cuts at: 2459 2459
Size: 6718
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6521
AflIII A'CryG_T
Cuts at: 1203 3030 5617 6594 1203
Size: 1827 2587 977 1327
Fragments arranged by size:
2587 1827 1327 977
AgeI A'CCGG_T
Cuts at: 5549 5549
Size: 6718
AhdI GACnn_n'nnGTC
Cuts at: 3304 5447 3304
Size: 2143 4575
AleI CACnn'nnGTG
Cuts at: 1166 3035 1166
Size: 1869 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3992 1184
Size: 2808 3910
AlwNI CAG_nnn'CTG
Cuts at: 2792 3271 3778 4461 6033 2792
Size: 479 507 683 1572 3477
Fragments arranged by size:
3477 1572 683 507 479
ApaI G_GGCC'C
Cuts at: 2178 2352 2369 2556 2178
Size: 174 17 187 6340
Fragments arranged by size:
6340 187 174 17
ApaLI G'TGCA_C
Cuts at: 3821 4775 5931 3821
Size: 954 1156 4608
Fragments arranged by size:
4608 1156 954
ApoI r'AATT_y
Cuts at: 1493 4027 4253 4405 6514 1493
Size: 2534 226 152 2109 1697
Fragments arranged by size:
2534 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5272 160
Size: 872 4240 1606
Fragments arranged by size:
4240 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2177 2247 3806 4043 1870
Size: 73 163 71 70 1559 237 4545
Fragments arranged by size:
4545 1559 237 163 73 71 70
BamHI G'GATC_C
Cuts at: 4048 4415 4048
Size: 367 6351
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 2717 4039 5494 618
Size: 325 217 627 225 705 1322 1455 1842
Fragments arranged by size:
1842 1455 1322 705 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 2178 2217 2294 2352 2369 2556 3134 4037
Size: 787 39 77 58 17 187 578 903
Cuts at: 4037 1391
Size: 4072
Fragments arranged by size:
4072 903 787 578 187 77 58 39
17
BbeI G_GCGC'C
Cuts at: 2721 2721
Size: 6718
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3649 928
Size: 971 1750 3997
Fragments arranged by size:
3997 1750 971
BbvCI CC'TCA_GC
Cuts at: 2778 2778
Size: 6718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2855 2889 3385 3419 4909 4943 1903
Size: 34 918 34 496 34 1490 34 3678
Fragments arranged by size:
3678 1490 918 496 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3588 4610 5820 3588
Size: 1022 1210 4486
Fragments arranged by size:
4486 1210 1022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6718
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2243 2863 5329
Size: 107 122 71 631 605 571 620 2466
Cuts at: 5329 136
Size: 1525
Fragments arranged by size:
2466 1525 631 620 605 571 122 107
71
BglII A'GATC_T
Cuts at: 6713 6713
Size: 6718
BlpI GC'TnA_GC
Cuts at: 2211 4135 2211
Size: 1924 4794
Bme1580I G_kGCm'C
Cuts at: 1790 2017 2178 2352 2369 2556 3825 4779 5935
Size: 227 161 174 17 187 1269 954 1156
Cuts at: 5935 1790
Size: 2573
Fragments arranged by size:
2573 1269 1156 954 227 187 174 161
17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6718
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2573 4010 4449
Size: 416 53 83 399 24 1156 1437 439
Cuts at: 4449 5407 442
Size: 958 1753
Fragments arranged by size:
1753 1437 1156 958 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2942 3029 3245 3835 5378 1239
Size: 1340 363 87 216 590 1543 2579
Fragments arranged by size:
2579 1543 1340 590 363 216 87
Bpu10I CC'TnA_GC
Cuts at: 2288 2778 3128 2288
Size: 490 350 5878
Fragments arranged by size:
5878 490 350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 2435 3593 4843 5708 6006 2348
Size: 87 1158 1250 865 298 3060
Fragments arranged by size:
3060 1250 1158 865 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 5381 882
Size: 617 1056 2826 2219
Fragments arranged by size:
2826 2219 1056 617
BsaAI yAC'GTr
Cuts at: 493 1204 4509 493
Size: 711 3305 2702
Fragments arranged by size:
3305 2702 711
BsaBI GATnn'nnATC
Cuts at: 6712 6712
Size: 6718
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2718 2988 4525 4907
Size: 53 83 186 936 1185 270 1537 382
Cuts at: 4907 5542 275
Size: 635 1451
Fragments arranged by size:
1537 1451 1185 936 635 382 270 186
83 53
BsaWI w'CCGG_w
Cuts at: 2991 3502 5151 5549 5823 5970 6381 2991
Size: 511 1649 398 274 147 411 3328
Fragments arranged by size:
3328 1649 511 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2021 2051 2138 2168 2525
Size: 30 165 30 618 30 87 30 357
Cuts at: 2525 2552 2555 2582 2585 2591 2615 2621 2808
Size: 27 3 27 3 6 24 6 187
Cuts at: 2808 2838 3017 3047 3746 3776 1178
Size: 30 179 30 699 30 4120
Fragments arranged by size:
4120 699 618 357 187 179 165 87
30 30 30 30 30 30 30 27
27 24 6 6 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2413 2425 2533 2599 2795 2832 2862 2919
Size: 1227 12 108 66 196 37 30 57
Cuts at: 2919 2922 3767 1186
Size: 3 845 4137
Fragments arranged by size:
4137 1227 845 196 108 66 57 37
30 12 3
BseYI C'CCAG_C
Cuts at: 2362 2549 2621 2797 2828 3087 3260 3284 3359
Size: 187 72 176 31 259 173 24 75
Cuts at: 3359 3888 3914 5921 6417 2362
Size: 529 26 2007 496 2663
Fragments arranged by size:
2663 2007 529 496 259 187 176 173
75 72 31 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2765 3600 3952 1163
Size: 573 1029 835 352 3929
Fragments arranged by size:
3929 1029 835 573 352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4168 4929 5957 1540
Size: 275 186 2167 761 1028 2301
Fragments arranged by size:
2301 2167 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 3813 3825 4779 4864 5935 2217
Size: 1596 12 954 85 1071 3000
Fragments arranged by size:
3000 1596 1071 954 85 12
BsmI GAATG_Cn'
Cuts at: 3128 4229 4322 3128
Size: 1101 93 5524
Fragments arranged by size:
5524 1101 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2052 2052
Size: 6718
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2178 2217 2294 2352 2369 2556
Size: 399 227 161 39 77 58 17 187
Cuts at: 2556 3134 3813 3825 4037 4779 4864 5935 1391
Size: 578 679 12 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 578 399 227 212
187 161 85 77 58 39 17 12
BspEI T'CCGG_A
Cuts at: 2991 3502 6381 2991
Size: 511 2879 3328
Fragments arranged by size:
3328 2879 511
BspHI T'CATG_A
Cuts at: 4607 6337 4607
Size: 1730 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3279 3741 4073 844
Size: 2435 462 332 3489
Fragments arranged by size:
3489 2435 462 332
BsrBI CCG'CTC
Cuts at: 1394 1823 4605 1394
Size: 429 2782 3507
Fragments arranged by size:
3507 2782 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3301 3372 5213 5387 65
Size: 1183 2053 71 1841 174 1396
Fragments arranged by size:
2053 1841 1396 1183 174 71
BsrFI r'CCGG_y
Cuts at: 1700 1810 2393 5362 5549 1700
Size: 110 583 2969 187 2869
Fragments arranged by size:
2969 2869 583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6718
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6718
BssSI C'ACGA_G
Cuts at: 4778 5790 4778
Size: 1012 5706
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2970 794
Size: 2176 4542
BstBI TT'CG_AA
Cuts at: 2887 5522 2887
Size: 2635 4083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6718
BstXI CCAn_nnnn'nTGG
Cuts at: 3007 3835 3007
Size: 828 5890
BstYI r'GATC_y
Cuts at: 1972 4048 4415 4800 4817 6269 6355 6367 6713
Size: 2076 367 385 17 1452 86 12 346
Cuts at: 6713 1972
Size: 1977
Fragments arranged by size:
2076 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2503 3589 3649 513
Size: 552 1438 1086 60 3582
Fragments arranged by size:
3582 1438 1086 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5979
Fragments arranged by size:
5979 683 56
BtsI GCAGTG_nn'
Cuts at: 2522 4234 5028 5048 2522
Size: 1712 794 20 4192
Fragments arranged by size:
4192 1712 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6683
DraI TTT'AAA
Cuts at: 4022 4022
Size: 6718
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5725 809
Size: 507 4409 1802
Fragments arranged by size:
4409 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2391 4165 5054
Size: 54 1425 50 275 18 561 1774 889
Cuts at: 5054 6683 8
Size: 1629 43
Fragments arranged by size:
1774 1629 1425 889 561 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4165 1537
Size: 275 2353 4090
Fragments arranged by size:
4090 2353 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2422 2748 2980 3435 4648 1353
Size: 869 200 326 232 455 1213 3423
Fragments arranged by size:
3423 1213 869 455 326 232 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5305 5679 5825 387
Size: 1064 138 3716 374 146 1280
Fragments arranged by size:
3716 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 6718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 2823 2942 3029 3245 3835 4781
Size: 393 947 244 119 87 216 590 946
Cuts at: 4781 5378 6165 6433 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 947 946 787 597 590 393 268
244 216 119 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2838 3308 3530 2838
Size: 470 222 6026
Fragments arranged by size:
6026 470 222
EcoO109I rG'GnC_Cy
Cuts at: 2034 2174 2349 2366 2386 2553 2573 3137 3189
Size: 140 175 17 20 167 20 564 52
Cuts at: 3189 3268 3325 3446 3519 2034
Size: 79 57 121 73 5233
Fragments arranged by size:
5233 564 175 167 140 121 79 73
57 52 20 20 17
EcoRI G'AATT_C
Cuts at: 4027 4405 4027
Size: 378 6340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 6718
FseI GG_CCGG'CC
Cuts at: 2397 2397
Size: 6718
FspI TGC'GCA
Cuts at: 5224 5224
Size: 6718
HaeII r_GCGC'y
Cuts at: 1678 2461 2721 5865 1678
Size: 783 260 3144 2531
Fragments arranged by size:
3144 2531 783 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2484 2516 2525
Size: 32 135 32 449 32 431 32 9
Cuts at: 2525 2552 2557 2584 2642 2674 2689 2721 2949
Size: 27 5 27 58 32 15 32 228
Cuts at: 2949 2981 3160 3192 3290 3322 3326 3358 3451
Size: 32 179 32 98 32 4 32 93
Cuts at: 3451 3483 3586 3618 5359 5391 5433 5465 1373
Size: 32 103 32 1741 32 42 32 2626
Fragments arranged by size:
2626 1741 449 431 228 179 135 103
98 93 58 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 27 27 15 9 5 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2533 4062 4308 669
Size: 988 281 595 1529 246 3079
Fragments arranged by size:
3079 1529 988 595 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6718
HpaI GTT'AAC
Cuts at: 4308 4308
Size: 6718
KasI G'GCGC_C
Cuts at: 2717 2717
Size: 6718
KpnI G_GTAC'C
Cuts at: 4043 4043
Size: 6718
MfeI C'AATT_G
Cuts at: 4317 4317
Size: 6718
MluI A'CGCG_T
Cuts at: 6594 6594
Size: 6718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2628 5832 6016 1305
Size: 1323 3204 184 2007
Fragments arranged by size:
3204 2007 1323 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4896
Fragments arranged by size:
4896 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 3957 3974 4676 5035 5194 518
Size: 648 1869 922 17 702 359 159 2042
Fragments arranged by size:
2042 1869 922 702 648 359 159 17
MspA1I CmG'CkG
Cuts at: 1606 2056 2549 4134 4811 5959 6204 1606
Size: 450 493 1585 677 1148 245 2120
Fragments arranged by size:
2120 1585 1148 677 493 450 245
NaeI GCC'GGC
Cuts at: 2395 2395
Size: 6718
NarI GG'CG_CC
Cuts at: 2718 2718
Size: 6718
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6166
NdeI CA'TA_TG
Cuts at: 387 3319 387
Size: 2932 3786
NgoMIV G'CCGG_C
Cuts at: 2393 2393
Size: 6718
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6718
NotI GC'GGCC_GC
Cuts at: 4165 4165
Size: 6718
NspI r_CATG'y
Cuts at: 1132 1229 3095 4076 5621 1132
Size: 97 1866 981 1545 2229
Fragments arranged by size:
2229 1866 1545 981 97
PasI CC'CwG_GG
Cuts at: 1348 2663 3185 1348
Size: 1315 522 4881
Fragments arranged by size:
4881 1315 522
PciI A'CATG_T
Cuts at: 5617 5617
Size: 6718
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6718
PmeI GTTT'AAAC
Cuts at: 4022 4022
Size: 6718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4787 4819 6300 6332 4787
Size: 32 1481 32 5173
Fragments arranged by size:
5173 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 3137 3189 3268 3446 3519 2386
Size: 751 52 79 178 73 5585
Fragments arranged by size:
5585 751 178 79 73 52
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6718
PsiI TTA'TAA
Cuts at: 4288 4288
Size: 6718
PspOMI G'GGCC_C
Cuts at: 2174 2348 2365 2552 2174
Size: 174 17 187 6340
Fragments arranged by size:
6340 187 174 17
PspXI vC'TCGA_Gb
Cuts at: 3806 3806
Size: 6718
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 6686
PstI C_TGCA'G
Cuts at: 830 2226 2339 2517 4070 830
Size: 1396 113 178 1553 3478
Fragments arranged by size:
3478 1553 1396 178 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6718
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 6718
SalI G'TCGA_C
Cuts at: 1936 4060 1936
Size: 2124 4594
SanDI GG'GwC_CC
Cuts at: 3446 3446
Size: 6718
SapI GCTCTTCn'nnn_
Cuts at: 2222 2748 2980 2222
Size: 526 232 5960
Fragments arranged by size:
5960 526 232
SbfI CC_TGCA'GG
Cuts at: 4070 4070
Size: 6718
ScaI AGT'ACT
Cuts at: 4966 6678 4966
Size: 1712 5006
SfcI C'TryA_G
Cuts at: 826 1045 2222 2335 2513 4066 5201 5882 6073
Size: 219 1177 113 178 1553 1135 681 191
Cuts at: 6073 826
Size: 1471
Fragments arranged by size:
1553 1471 1177 1135 681 219 191 178
113
SfoI GGC'GCC
Cuts at: 2719 2719
Size: 6718
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6718
SmaI CCC'GGG
Cuts at: 1872 2108 2179 2249 4045 1872
Size: 236 71 70 1796 4545
Fragments arranged by size:
4545 1796 236 71 70
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2450 3608 3806 4822 5723
Size: 197 926 384 123 1158 198 1016 901
Cuts at: 5723 5985 820
Size: 262 1553
Fragments arranged by size:
1553 1158 1016 926 901 384 262 198
197 123
SnaBI TAC'GTA
Cuts at: 493 4509 493
Size: 4016 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6718
SphI G_CATG'C
Cuts at: 1132 4076 1132
Size: 2944 3774
SrfI GCCC'GGGC
Cuts at: 2108 2249 2108
Size: 141 6577
SspI AAT'ATT
Cuts at: 5 52 4642 5
Size: 47 4590 2081
Fragments arranged by size:
4590 2081 47
StuI AGG'CCT
Cuts at: 1517 2945 3101 1517
Size: 1428 156 5134
Fragments arranged by size:
5134 1428 156
StyI C'CwwG_G
Cuts at: 513 1065 2940 3492 3581 4157 513
Size: 552 1875 552 89 576 3074
Fragments arranged by size:
3074 1875 576 552 552 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 2333 2953 3094 4745 4762 4915
Size: 1255 707 46 620 141 1651 17 153
Cuts at: 4915 5100 5545 325
Size: 185 445 1498
Fragments arranged by size:
1651 1498 1255 707 620 445 185 153
141 46 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4964 6676 96
Size: 274 80 33 51 1443 2987 1712 138
Fragments arranged by size:
2987 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2808 2840 3746 3778 6678 6710 2808
Size: 32 906 32 2900 32 2816
Fragments arranged by size:
2900 2816 906 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2566 2703 1316
Size: 1250 137 5331
Fragments arranged by size:
5331 1250 137
XbaI T'CTAG_A
Cuts at: 4054 4054
Size: 6718
XhoI C'TCGA_G
Cuts at: 1943 3806 1943
Size: 1863 4855
XmaI C'CCGG_G
Cuts at: 1870 2106 2177 2247 4043 1870
Size: 236 71 70 1796 4545
Fragments arranged by size:
4545 1796 236 71 70
XmnI GAAnn'nnTTC
Cuts at: 3405 4847 3405
Size: 1442 5276
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2989 4526 276
Size: 53 83 186 936 1455 1537 2468
Fragments arranged by size:
2468 1537 1455 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoRV FspAI NruI NsiI PacI PfoI
PmlI PvuII RsrII SacII SexAI SfiI SwaI XcmI