Restriction map for
pFN21AA1296
(Circular) (Six-base) MAPSORT of: pFN21AA1296.seq Check: 8207 from: 1 to: 7048
pFN21AA1296 7048 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 4858 278
Size: 53 83 186 936 1909 1413 2468
Fragments arranged by size:
2468 1909 1413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4391 1937
Size: 2454 4594
Acc65I G'GTAC_C
Cuts at: 4369 4369
Size: 7048
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2919 3038 3168 3308 3692 3892 5111
Size: 678 609 119 130 140 384 200 1219
Cuts at: 5111 6495 6763 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1219 678 609 384 268 200
140 130 119
AfeI AGC'GCT
Cuts at: 3572 3572
Size: 7048
AflII C'TTAA_G
Cuts at: 820 1017 2752 820
Size: 197 1735 5116
Fragments arranged by size:
5116 1735 197
AflIII A'CryG_T
Cuts at: 1203 5947 6924 1203
Size: 4744 977 1327
Fragments arranged by size:
4744 1327 977
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 5777 5777
Size: 7048
AleI CACnn'nnGTG
Cuts at: 1166 4010 1166
Size: 2844 4204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7048
AlwNI CAG_nnn'CTG
Cuts at: 2624 3331 3670 4791 6363 2624
Size: 707 339 1121 1572 3309
Fragments arranged by size:
3309 1572 1121 707 339
ApaLI G'TGCA_C
Cuts at: 5105 6261 5105
Size: 1156 5892
ApoI r'AATT_y
Cuts at: 1493 3177 3285 3428 3647 4357 4583 4735 6844
Size: 1684 108 143 219 710 226 152 2109
Cuts at: 6844 1493
Size: 1697
Fragments arranged by size:
2109 1697 1684 710 226 219 152 143
108
AseI AT'TA_AT
Cuts at: 160 1032 5602 160
Size: 872 4570 1606
Fragments arranged by size:
4570 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 3973 4373 1870
Size: 73 2030 400 4545
Fragments arranged by size:
4545 2030 400 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3879 3912 4325 4358 3879
Size: 33 413 33 6569
Fragments arranged by size:
6569 413 33 33
BamHI G'GATC_C
Cuts at: 2182 3969 4378 4745 2182
Size: 1787 409 367 4485
Fragments arranged by size:
4485 1787 409 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 4369 5824 618
Size: 325 217 627 266 2316 1455 1842
Fragments arranged by size:
2316 1842 1455 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2273 3093 3155 3250 3342 4367 1391
Size: 882 820 62 95 92 1025 4072
Fragments arranged by size:
4072 1025 882 820 95 92 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 2226 2907 3117 3445 928
Size: 971 245 82 681 210 328 4531
Fragments arranged by size:
4531 971 681 328 245 210 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2567 2601 3766 3800 5239 5273 1903
Size: 34 630 34 1165 34 1439 34 3678
Fragments arranged by size:
3678 1439 1165 630 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4940 6150 4940
Size: 1210 5838
BclI T'GATC_A
Cuts at: 1548 3724 4020 1548
Size: 2176 296 4576
Fragments arranged by size:
4576 2176 296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3531 5659 136
Size: 107 122 71 631 605 1859 2128 1525
Fragments arranged by size:
2128 1859 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2869 7043 2869
Size: 4174 2874
BlpI GC'TnA_GC
Cuts at: 3154 4465 3154
Size: 1311 5737
Bme1580I G_kGCm'C
Cuts at: 1790 5109 6265 1790
Size: 3319 1156 2573
Fragments arranged by size:
3319 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7048
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2211 3052 4779
Size: 416 53 83 399 24 794 841 1727
Cuts at: 4779 5737 442
Size: 958 1753
Fragments arranged by size:
1753 1727 958 841 794 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 7048
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 5708 1239
Size: 1091 3378 2579
Fragments arranged by size:
3378 2579 1091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3326 4101 5173 6038 6336 3326
Size: 775 1072 865 298 4038
Fragments arranged by size:
4038 1072 865 775 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3625 5711 882
Size: 617 2126 2086 2219
Fragments arranged by size:
2219 2126 2086 617
BsaAI yAC'GTr
Cuts at: 493 1204 4012 4839 493
Size: 711 2808 827 2702
Fragments arranged by size:
2808 2702 827 711
BsaBI GATnn'nnATC
Cuts at: 2868 7042 2868
Size: 4174 2874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3523 3534 4855
Size: 53 83 186 936 1909 81 11 1321
Cuts at: 4855 5237 5872 275
Size: 382 635 1451
Fragments arranged by size:
1909 1451 1321 936 635 382 186 83
81 53 11
BsaWI w'CCGG_w
Cuts at: 2790 3172 5481 5879 6153 6300 6711 2790
Size: 382 2309 398 274 147 411 3127
Fragments arranged by size:
3127 2309 411 398 382 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2896 2926 2979 3009 3687
Size: 30 165 30 1493 30 53 30 678
Cuts at: 3687 3717 4227 4257 1178
Size: 30 510 30 3969
Fragments arranged by size:
3969 1493 678 510 165 53 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3575 4063 4066 1186
Size: 2389 488 3 4168
Fragments arranged by size:
4168 2389 488 3
BseYI C'CCAG_C
Cuts at: 2376 2998 4122 6251 6747 2376
Size: 622 1124 2129 496 2677
Fragments arranged by size:
2677 2129 1124 622 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2182 3105 3857 1163
Size: 573 446 923 752 4354
Fragments arranged by size:
4354 923 752 573 446
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4498 5259 6287 1540
Size: 275 186 1756 741 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 741 275 186
BsiHKAI G_wGCw'C
Cuts at: 2574 2744 3155 5109 5194 6265 2574
Size: 170 411 1954 85 1071 3357
Fragments arranged by size:
3357 1954 1071 411 170 85
BsmI GAATG_Cn'
Cuts at: 4559 4652 4559
Size: 93 6955
BsmBI CGTCTCn'nnnn_
Cuts at: 3472 3472
Size: 7048
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 2574 2744 3093 3155 3250 3342
Size: 399 483 301 170 349 62 95 92
Cuts at: 3342 4367 5109 5194 6265 1391
Size: 1025 742 85 1071 2174
Fragments arranged by size:
2174 1071 1025 742 483 399 349 301
170 95 92 85 62
BspEI T'CCGG_A
Cuts at: 2790 3172 6711 2790
Size: 382 3539 3127
Fragments arranged by size:
3539 3127 382
BspHI T'CATG_A
Cuts at: 3445 4937 6667 3445
Size: 1492 1730 3826
Fragments arranged by size:
3826 1730 1492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3643 3829 3931 4071 4403 844
Size: 2799 186 102 140 332 3489
Fragments arranged by size:
3489 2799 332 186 140 102
BsrBI CCG'CTC
Cuts at: 1394 1823 2213 3006 4935 1394
Size: 429 390 793 1929 3507
Fragments arranged by size:
3507 1929 793 429 390
BsrDI GCAATG_nn'
Cuts at: 65 1248 2071 5543 5717 65
Size: 1183 823 3472 174 1396
Fragments arranged by size:
3472 1396 1183 823 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5692 5879 1700
Size: 110 3882 187 2869
Fragments arranged by size:
3882 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7048
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7048
BssSI C'ACGA_G
Cuts at: 5108 6120 5108
Size: 1012 6036
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7048
BstBI TT'CG_AA
Cuts at: 3436 5852 3436
Size: 2416 4632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7048
BstYI r'GATC_y
Cuts at: 1972 2182 2620 2838 2869 3256 3969 4378 4745
Size: 210 438 218 31 387 713 409 367
Cuts at: 4745 5130 5147 6599 6685 6697 7043 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 713 438 409 387 385 367
346 218 210 86 31 17 12
Bsu36I CC'TnA_GG
Cuts at: 2155 2274 2155
Size: 119 6929
BtgI C'CryG_G
Cuts at: 513 1065 2766 3420 3751 513
Size: 552 1701 654 331 3810
Fragments arranged by size:
3810 1701 654 552 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2772 4245 487
Size: 683 56 1546 1473 3290
Fragments arranged by size:
3290 1546 1473 683 56
BtsI GCAGTG_nn'
Cuts at: 3231 3929 4095 4096 4564 5358 5378 3231
Size: 698 166 1 468 794 20 4901
Fragments arranged by size:
4901 794 698 468 166 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2533 2568 529
Size: 35 1969 35 5009
Fragments arranged by size:
5009 1969 35 35
DraI TTT'AAA
Cuts at: 3658 4352 3658
Size: 694 6354
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6055 809
Size: 507 4739 1802
Fragments arranged by size:
4739 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2982 3754 4495
Size: 54 1425 50 275 18 1152 772 741
Cuts at: 4495 5384 7013 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1152 889 772 741 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 4495 1537
Size: 275 1942 741 4090
Fragments arranged by size:
4090 1942 741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2389 2632 2732 3013 3224 3327 3495 4126
Size: 1036 243 100 281 211 103 168 631
Cuts at: 4126 4978 1353
Size: 852 3423
Fragments arranged by size:
3423 1036 852 631 281 243 211 168
103 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2521 5635 6009 6155 387
Size: 1064 138 932 3114 374 146 1280
Fragments arranged by size:
3114 1280 1064 932 374 146 138
EcoICRI GAG'CTC
Cuts at: 3153 3153
Size: 7048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2330 2919 3038 3168 3308 3692
Size: 393 678 20 589 119 130 140 384
Cuts at: 3692 3892 5111 5708 6495 6763 1239
Size: 200 1219 597 787 268 1524
Fragments arranged by size:
1524 1219 787 678 597 589 393 384
268 200 140 130 119 20
EcoO109I rG'GnC_Cy
Cuts at: 3328 3580 3328
Size: 252 6796
EcoRI G'AATT_C
Cuts at: 4357 4735 4357
Size: 378 6670
EcoRV GAT'ATC
Cuts at: 4240 4240
Size: 7048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3479 3892 3479
Size: 413 6635
FspI TGC'GCA
Cuts at: 5554 5554
Size: 7048
HaeII r_GCGC'y
Cuts at: 1678 3574 6195 1678
Size: 1896 2621 2531
Fragments arranged by size:
2621 2531 1896
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 2612 2644 2861
Size: 32 135 32 970 32 38 32 217
Cuts at: 2861 2893 2894 2926 3365 3397 3535 3567 3978
Size: 32 1 32 439 32 138 32 411
Cuts at: 3978 4010 5689 5721 5763 5795 1373
Size: 32 1679 32 42 32 2626
Fragments arranged by size:
2626 1679 970 439 411 217 138 135
42 38 32 32 32 32 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4392 4638 669
Size: 988 281 1890 564 246 3079
Fragments arranged by size:
3079 1890 988 564 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7048
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KpnI G_GTAC'C
Cuts at: 4373 4373
Size: 7048
MfeI C'AATT_G
Cuts at: 4647 4647
Size: 7048
MluI A'CGCG_T
Cuts at: 6924 6924
Size: 7048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3237 3566 4200 6162 6346 1305
Size: 1932 329 634 1962 184 2007
Fragments arranged by size:
2007 1962 1932 634 329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5226
Fragments arranged by size:
5226 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 4010 5006 5365 5524 518
Size: 648 966 1878 996 359 159 2042
Fragments arranged by size:
2042 1878 996 966 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3404 4464 5141 6289 6534 1606
Size: 1798 1060 677 1148 245 2120
Fragments arranged by size:
2120 1798 1148 1060 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2766 513
Size: 552 1701 4795
Fragments arranged by size:
4795 1701 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7048
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NspI r_CATG'y
Cuts at: 1132 1229 4406 5951 1132
Size: 97 3177 1545 2229
Fragments arranged by size:
3177 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7048
PciI A'CATG_T
Cuts at: 5947 5947
Size: 7048
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7048
PfoI T'CCnGG_A
Cuts at: 3193 3193
Size: 7048
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 6630 6662 5117
Size: 32 1481 32 5503
Fragments arranged by size:
5503 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3580 3580
Size: 7048
PshAI GACnn'nnGTC
Cuts at: 1613 4149 1613
Size: 2536 4512
PsiI TTA'TAA
Cuts at: 3291 3714 4618 3291
Size: 423 904 5721
Fragments arranged by size:
5721 904 423
PstI C_TGCA'G
Cuts at: 830 3690 4400 830
Size: 2860 710 3478
Fragments arranged by size:
3478 2860 710
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
PvuII CAG'CTG
Cuts at: 3404 3404
Size: 7048
SacI G_AGCT'C
Cuts at: 3155 3155
Size: 7048
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SapI GCTCTTCn'nnn_
Cuts at: 2732 3013 2732
Size: 281 6767
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 5296 7008 5296
Size: 1712 5336
SexAI A'CCwGG_T
Cuts at: 2302 2302
Size: 7048
SfcI C'TryA_G
Cuts at: 826 1045 2172 2239 2404 3686 4396 5531 6212
Size: 219 1127 67 165 1282 710 1135 681
Cuts at: 6212 6403 826
Size: 191 1471
Fragments arranged by size:
1471 1282 1135 1127 710 681 219 191
165 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7048
SmaI CCC'GGG
Cuts at: 1872 3975 4375 1872
Size: 2103 400 4545
Fragments arranged by size:
4545 2103 400
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2752 3341 4116 5152 6053 6315
Size: 197 926 809 589 775 1036 901 262
Cuts at: 6315 820
Size: 1553
Fragments arranged by size:
1553 1036 926 901 809 775 589 262
197
SnaBI TAC'GTA
Cuts at: 493 4839 493
Size: 4346 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7048
SphI G_CATG'C
Cuts at: 1132 4406 1132
Size: 3274 3774
SspI AAT'ATT
Cuts at: 5 52 4972 5
Size: 47 4920 2081
Fragments arranged by size:
4920 2081 47
StuI AGG'CCT
Cuts at: 1517 2160 1517
Size: 643 6405
StyI C'CwwG_G
Cuts at: 513 1065 2397 2766 4487 513
Size: 552 1332 369 1721 3074
Fragments arranged by size:
3074 1721 1332 552 369
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5075 5092 5245 5430 5875 325
Size: 1255 3495 17 153 185 445 1498
Fragments arranged by size:
3495 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2874 5294 7006
Size: 274 80 33 51 1443 897 2420 1712
Cuts at: 7006 96
Size: 138
Fragments arranged by size:
2420 1712 1443 897 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2896 2928 2979 3011 7008 7040 2896
Size: 32 51 32 3997 32 2904
Fragments arranged by size:
3997 2904 51 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7048
XbaI T'CTAG_A
Cuts at: 4384 4384
Size: 7048
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 4009 2773
Size: 1236 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7048
XmaI C'CCGG_G
Cuts at: 1870 3973 4373 1870
Size: 2103 400 4545
Fragments arranged by size:
4545 2103 400
XmnI GAAnn'nnTTC
Cuts at: 2494 3432 5177 2494
Size: 938 1745 4365
Fragments arranged by size:
4365 1745 938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 4856 276
Size: 53 83 186 936 1909 1413 2468
Fragments arranged by size:
2468 1909 1413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI