Restriction map for
pFN21AA1292
(Circular) (Six-base) MAPSORT of: pFN21AA1292.seq Check: 9176 from: 1 to: 6811
pFN21AA1292 6811 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2464 2878 3826 2464
Size: 414 948 5449
Fragments arranged by size:
5449 948 414
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3695 3740 4621 278
Size: 53 83 186 936 2159 45 881 2468
Fragments arranged by size:
2468 2159 936 881 186 83 53 45
AccI GT'mk_AC
Cuts at: 1937 2322 4154 1937
Size: 385 1832 4594
Fragments arranged by size:
4594 1832 385
Acc65I G'GTAC_C
Cuts at: 4132 4132
Size: 6811
AclI AA'CG_TT
Cuts at: 1563 4938 5311 1563
Size: 3375 373 3063
Fragments arranged by size:
3375 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2705 3373 3977 4874 6258 6526 1632
Size: 1073 668 604 897 1384 268 1917
Fragments arranged by size:
1917 1384 1073 897 668 604 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6614
AflIII A'CryG_T
Cuts at: 1203 5710 6687 1203
Size: 4507 977 1327
Fragments arranged by size:
4507 1327 977
AgeI A'CCGG_T
Cuts at: 5642 5642
Size: 6811
AhdI GACnn_n'nnGTC
Cuts at: 2998 3445 5540 2998
Size: 447 2095 4269
Fragments arranged by size:
4269 2095 447
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2819 1184
Size: 1635 5176
AlwNI CAG_nnn'CTG
Cuts at: 2181 2951 3166 3832 4554 6126 2181
Size: 770 215 666 722 1572 2866
Fragments arranged by size:
2866 1572 770 722 666 215
ApaI G_GGCC'C
Cuts at: 3186 3407 3605 3657 3789 3878 4035 3186
Size: 221 198 52 132 89 157 5962
Fragments arranged by size:
5962 221 198 157 132 89 52
ApaLI G'TGCA_C
Cuts at: 2286 3726 4868 6024 2286
Size: 1440 1142 1156 3073
Fragments arranged by size:
3073 1440 1156 1142
ApoI r'AATT_y
Cuts at: 1493 4120 4346 4498 6607 1493
Size: 2627 226 152 2109 1697
Fragments arranged by size:
2627 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5365 160
Size: 872 4333 1606
Fragments arranged by size:
4333 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2451 2514 3094 3127 3414 3606
Size: 73 94 414 63 580 33 287 192
Cuts at: 3606 3781 3991 4136 1870
Size: 175 210 145 4545
Fragments arranged by size:
4545 580 414 287 210 192 175 145
94 73 63 33
BamHI G'GATC_C
Cuts at: 4141 4508 4141
Size: 367 6444
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2560 2914 3668 4132 5587
Size: 325 217 627 773 354 754 464 1455
Cuts at: 5587 618
Size: 1842
Fragments arranged by size:
1842 1455 773 754 627 464 354 325
217
BanII G_rGCy'C
Cuts at: 1391 2080 3186 3407 3423 3605 3657 3789 3878
Size: 689 1106 221 16 182 52 132 89
Cuts at: 3878 4035 4130 1391
Size: 157 95 4072
Fragments arranged by size:
4072 1106 689 221 182 157 132 95
89 52 16
BbeI G_GCGC'C
Cuts at: 2564 3672 2564
Size: 1108 5703
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3965 928
Size: 971 2066 3774
Fragments arranged by size:
3774 2066 971
BbvCI CC'TCA_GC
Cuts at: 2340 3951 2340
Size: 1611 5200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5002 5036 1903
Size: 34 3065 34 3678
Fragments arranged by size:
3678 3065 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2120 3317 4703 5913 2120
Size: 1197 1386 1210 3018
Fragments arranged by size:
3018 1386 1210 1197
BclI T'GATC_A
Cuts at: 1548 3760 1548
Size: 2212 4599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2427 2587 3138
Size: 107 122 71 631 605 755 160 551
Cuts at: 3138 5422 136
Size: 2284 1525
Fragments arranged by size:
2284 1525 755 631 605 551 160 122
107 71
BglII A'GATC_T
Cuts at: 4101 6806 4101
Size: 2705 4106
BlpI GC'TnA_GC
Cuts at: 3074 4228 3074
Size: 1154 5657
Bme1580I G_kGCm'C
Cuts at: 1790 2290 2839 2919 3186 3407 3605 3657 3730
Size: 500 549 80 267 221 198 52 73
Cuts at: 3730 3789 3878 4035 4872 6028 1790
Size: 59 89 157 837 1156 2573
Fragments arranged by size:
2573 1156 837 549 500 267 221 198
157 89 80 73 59 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6811
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2490 4542 5500
Size: 416 53 83 399 24 1073 2052 958
Cuts at: 5500 442
Size: 1753
Fragments arranged by size:
2052 1753 1073 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3616 5471 1239
Size: 2377 1855 2579
Fragments arranged by size:
2579 2377 1855
Bpu10I CC'TnA_GC
Cuts at: 2340 3113 3951 2340
Size: 773 838 5200
Fragments arranged by size:
5200 838 773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 3104 4936 5801 6099 2642
Size: 462 1832 865 298 3354
Fragments arranged by size:
3354 1832 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5474 882
Size: 617 3975 2219
Fragments arranged by size:
3975 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2139 2326 2542 2572 4602 493
Size: 711 935 187 216 30 2030 2702
Fragments arranged by size:
2702 2030 935 711 216 187 30
BsaBI GATnn'nnATC
Cuts at: 6805 6805
Size: 6811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2242 2301 2561 3546
Size: 53 83 186 936 709 59 260 985
Cuts at: 3546 3669 3692 3737 4618 5000 5635 275
Size: 123 23 45 881 382 635 1451
Fragments arranged by size:
1451 985 936 881 709 635 382 260
186 123 83 59 53 45 23
BsaWI w'CCGG_w
Cuts at: 5244 5642 5916 6063 6474 5244
Size: 398 274 147 411 5581
Fragments arranged by size:
5581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6586
Fragments arranged by size:
6586 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3506 3736 1186
Size: 2320 230 4261
Fragments arranged by size:
4261 2320 230
BseYI C'CCAG_C
Cuts at: 2347 3304 3400 3560 3650 3794 4006 6014 6510
Size: 957 96 160 90 144 212 2008 496
Cuts at: 6510 2347
Size: 2648
Fragments arranged by size:
2648 2008 957 496 212 160 144 96
90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2634 2867 3804 3956 1163
Size: 573 898 233 937 152 4018
Fragments arranged by size:
4018 937 898 573 233 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4261 5022 6050 1540
Size: 275 186 2260 761 1028 2301
Fragments arranged by size:
2301 2260 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2290 2541 3730 4872 4957 6028 2290
Size: 251 1189 1142 85 1071 3073
Fragments arranged by size:
3073 1189 1142 1071 251 85
BsmI GAATG_Cn'
Cuts at: 4322 4415 4322
Size: 93 6718
BsmBI CGTCTCn'nnnn_
Cuts at: 3630 3630
Size: 6811
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2290 2541 2839 2919 3186 3407
Size: 399 290 210 251 298 80 267 221
Cuts at: 3407 3423 3605 3657 3730 3789 3878 4035 4130
Size: 16 182 52 73 59 89 157 95
Cuts at: 4130 4872 4957 6028 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 298 290 267 251
221 210 182 157 95 89 85 80
73 59 52 16
BspEI T'CCGG_A
Cuts at: 6474 6474
Size: 6811
BspHI T'CATG_A
Cuts at: 4700 6430 4700
Size: 1730 5081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2464 2811 2878 2929 3348 3826 4021 4166
Size: 1620 347 67 51 419 478 195 145
Cuts at: 4166 844
Size: 3489
Fragments arranged by size:
3489 1620 478 419 347 195 145 67
51
BsrBI CCG'CTC
Cuts at: 1394 1823 2042 2596 2656 3073 4698 1394
Size: 429 219 554 60 417 1625 3507
Fragments arranged by size:
3507 1625 554 429 417 219 60
BsrDI GCAATG_nn'
Cuts at: 65 1248 5306 5480 65
Size: 1183 4058 174 1396
Fragments arranged by size:
4058 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2393 2727 2890 3132 4019 5455 5642
Size: 110 583 334 163 242 887 1436 187
Cuts at: 5642 1700
Size: 2869
Fragments arranged by size:
2869 1436 887 583 334 242 187 163
110
BsrGI T'GTAC_A
Cuts at: 96 2085 2877 96
Size: 1989 792 4030
Fragments arranged by size:
4030 1989 792
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6811
BssSI C'ACGA_G
Cuts at: 4871 5883 4871
Size: 1012 5799
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3166 794
Size: 2372 4439
BstBI TT'CG_AA
Cuts at: 5615 5615
Size: 6811
BstEII G'GTnAC_C
Cuts at: 1184 3813 1184
Size: 2629 4182
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2374 2017
Size: 357 6454
BstYI r'GATC_y
Cuts at: 1972 4101 4141 4508 4893 4910 6362 6448 6460
Size: 2129 40 367 385 17 1452 86 12
Cuts at: 6460 6806 1972
Size: 346 1977
Fragments arranged by size:
2129 1977 1452 385 367 346 86 40
17 12
Bsu36I CC'TnA_GG
Cuts at: 2624 2624
Size: 6811
BtgI C'CryG_G
Cuts at: 513 1065 2004 2373 2491 2901 3368 3731 513
Size: 552 939 369 118 410 467 363 3593
Fragments arranged by size:
3593 939 552 467 410 369 363 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6072
Fragments arranged by size:
6072 683 56
BtsI GCAGTG_nn'
Cuts at: 2170 2212 2762 2803 2830 3221 3805 4327 5121
Size: 42 550 41 27 391 584 522 794
Cuts at: 5121 5141 2170
Size: 20 3840
Fragments arranged by size:
3840 794 584 550 522 391 42 41
27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2733 2768 529
Size: 35 2169 35 4572
Fragments arranged by size:
4572 2169 35 35
DraI TTT'AAA
Cuts at: 4115 4115
Size: 6811
DraIII CAC_nnn'GTG
Cuts at: 2285 2296 2285
Size: 11 6800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3545 3697 5818 809
Size: 507 2229 152 2121 1802
Fragments arranged by size:
2229 2121 1802 507 152
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2391 3283
Size: 54 1425 50 275 18 177 384 892
Cuts at: 3283 3318 4258 5147 6776 8
Size: 35 940 889 1629 43
Fragments arranged by size:
1629 1425 940 892 889 384 275 177
54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 4258 1537
Size: 275 2446 4090
Fragments arranged by size:
4090 2446 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 3536 4741 1353
Size: 1052 1131 1205 3423
Fragments arranged by size:
3423 1205 1131 1052
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5398 5772 5918 387
Size: 1064 138 3809 374 146 1280
Fragments arranged by size:
3809 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2705 3373 3616 3977 4874 5471 6258
Size: 393 1073 668 243 361 897 597 787
Cuts at: 6258 6526 1239
Size: 268 1524
Fragments arranged by size:
1524 1073 897 787 668 597 393 361
268 243
EcoNI CCTnn'n_nnAGG
Cuts at: 3578 3578
Size: 6811
EcoO109I rG'GnC_Cy
Cuts at: 2906 2948 3040 3182 3183 3404 3601 3602 3611
Size: 42 92 142 1 221 197 1 9
Cuts at: 3611 3785 3786 3829 3875 4010 4031 4032 2906
Size: 174 1 43 46 135 21 1 5685
Fragments arranged by size:
5685 221 197 174 142 135 92 46
43 42 21 9 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4120 4498 4120
Size: 378 6433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 6811
FseI GG_CCGG'CC
Cuts at: 2397 2397
Size: 6811
FspI TGC'GCA
Cuts at: 5317 5317
Size: 6811
HaeII r_GCGC'y
Cuts at: 1678 2564 3081 3102 3505 3672 5958 1678
Size: 886 517 21 403 167 2286 2531
Fragments arranged by size:
2531 2286 886 517 403 167 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2984 3016 3077 3109 3431
Size: 32 135 32 1412 32 61 32 322
Cuts at: 3431 3463 5452 5484 5526 5558 1373
Size: 32 1989 32 42 32 2626
Fragments arranged by size:
2626 1989 1412 322 135 61 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 4155 4401 669
Size: 988 281 289 1928 246 3079
Fragments arranged by size:
3079 1928 988 289 281 246
HindIII A'AGCT_T
Cuts at: 748 2666 3974 748
Size: 1918 1308 3585
Fragments arranged by size:
3585 1918 1308
HpaI GTT'AAC
Cuts at: 4401 4401
Size: 6811
KasI G'GCGC_C
Cuts at: 2560 3668 2560
Size: 1108 5703
KpnI G_GTAC'C
Cuts at: 4136 4136
Size: 6811
MfeI C'AATT_G
Cuts at: 4410 4410
Size: 6811
MluI A'CGCG_T
Cuts at: 6687 6687
Size: 6811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5925 6109 1305
Size: 4620 184 2007
Fragments arranged by size:
4620 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3285 10
Size: 54 1425 343 177 1276 3536
Fragments arranged by size:
3536 1425 1276 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2372 2387 4769 5128 5287 518
Size: 648 849 357 15 2382 359 159 2042
Fragments arranged by size:
2382 2042 849 648 359 357 159 15
MspA1I CmG'CkG
Cuts at: 1606 2083 2493 3733 3955 4227 4904 6052 6297
Size: 477 410 1240 222 272 677 1148 245
Cuts at: 6297 1606
Size: 2120
Fragments arranged by size:
2120 1240 1148 677 477 410 272 245
222
NaeI GCC'GGC
Cuts at: 2395 2892 3134 2395
Size: 497 242 6072
Fragments arranged by size:
6072 497 242
NarI GG'CG_CC
Cuts at: 2561 3669 2561
Size: 1108 5703
NcoI C'CATG_G
Cuts at: 513 1065 2004 2373 513
Size: 552 939 369 4951
Fragments arranged by size:
4951 939 552 369
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6811
NgoMIV G'CCGG_C
Cuts at: 2393 2890 3132 2393
Size: 497 242 6072
Fragments arranged by size:
6072 497 242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6811
NotI GC'GGCC_GC
Cuts at: 4258 4258
Size: 6811
NspI r_CATG'y
Cuts at: 1132 1229 3050 4169 5714 1132
Size: 97 1821 1119 1545 2229
Fragments arranged by size:
2229 1821 1545 1119 97
PasI CC'CwG_GG
Cuts at: 1348 2220 2965 4028 1348
Size: 872 745 1063 4131
Fragments arranged by size:
4131 1063 872 745
PciI A'CATG_T
Cuts at: 5710 5710
Size: 6811
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3311 1108
Size: 2203 4608
PfoI T'CCnGG_A
Cuts at: 2637 3449 3471 2637
Size: 812 22 5977
Fragments arranged by size:
5977 812 22
PmeI GTTT'AAAC
Cuts at: 4115 4115
Size: 6811
PmlI CAC'GTG
Cuts at: 2139 2542 2139
Size: 403 6408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4880 4912 6393 6425 4880
Size: 32 1481 32 5266
Fragments arranged by size:
5266 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2906 3611 4010 2906
Size: 705 399 5707
Fragments arranged by size:
5707 705 399
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6811
PsiI TTA'TAA
Cuts at: 4381 4381
Size: 6811
PspOMI G'GGCC_C
Cuts at: 3182 3403 3601 3653 3785 3874 4031 3182
Size: 221 198 52 132 89 157 5962
Fragments arranged by size:
5962 221 198 157 132 89 52
PspXI vC'TCGA_Gb
Cuts at: 3127 3127
Size: 6811
PstI C_TGCA'G
Cuts at: 830 2177 2808 3162 3579 3690 3756 4163 830
Size: 1347 631 354 417 111 66 407 3478
Fragments arranged by size:
3478 1347 631 417 407 354 111 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6811
PvuII CAG'CTG
Cuts at: 3955 3955
Size: 6811
SacII CC_GC'GG
Cuts at: 2494 3734 2494
Size: 1240 5571
SalI G'TCGA_C
Cuts at: 1936 4153 1936
Size: 2217 4594
SanDI GG'GwC_CC
Cuts at: 2906 2906
Size: 6811
SbfI CC_TGCA'GG
Cuts at: 3756 4163 3756
Size: 407 6404
ScaI AGT'ACT
Cuts at: 5059 6771 5059
Size: 1712 5099
SfcI C'TryA_G
Cuts at: 826 1045 2173 2804 3158 3568 3575 3686 3752
Size: 219 1128 631 354 410 7 111 66
Cuts at: 3752 4159 5294 5975 6166 826
Size: 407 1135 681 191 1471
Fragments arranged by size:
1471 1135 1128 681 631 410 407 354
219 191 111 66 7
SfoI GGC'GCC
Cuts at: 2562 3670 2562
Size: 1108 5703
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6811
SmaI CCC'GGG
Cuts at: 1872 3416 3783 3993 4138 1872
Size: 1544 367 210 145 4545
Fragments arranged by size:
4545 1544 367 210 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 3119 3127 4915 5816 6078
Size: 197 926 714 462 8 1788 901 262
Cuts at: 6078 820
Size: 1553
Fragments arranged by size:
1788 1553 926 901 714 462 262 197
8
SnaBI TAC'GTA
Cuts at: 493 4602 493
Size: 4109 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6811
SphI G_CATG'C
Cuts at: 1132 3050 4169 1132
Size: 1918 1119 3774
Fragments arranged by size:
3774 1918 1119
SspI AAT'ATT
Cuts at: 5 52 4735 5
Size: 47 4683 2081
Fragments arranged by size:
4683 2081 47
StuI AGG'CCT
Cuts at: 1517 2629 3125 1517
Size: 1112 496 5203
Fragments arranged by size:
5203 1112 496
StyI C'CwwG_G
Cuts at: 513 1065 2004 2373 2712 2910 4250 513
Size: 552 939 369 339 198 1340 3074
Fragments arranged by size:
3074 1340 939 552 369 339 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2658 3318 4073 4838 4855 5008 5193
Size: 1255 1078 660 755 765 17 153 185
Cuts at: 5193 5638 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1078 765 755 660 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 2877 5057
Size: 274 80 33 51 1443 108 792 2180
Cuts at: 5057 6769 96
Size: 1712 138
Fragments arranged by size:
2180 1712 1443 792 274 138 108 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 6771 6803 3848
Size: 32 2891 32 3856
Fragments arranged by size:
3856 2891 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3739 1316
Size: 2423 4388
XbaI T'CTAG_A
Cuts at: 4147 4147
Size: 6811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2719 3308 3457 2371
Size: 348 589 149 5725
Fragments arranged by size:
5725 589 348 149
XhoI C'TCGA_G
Cuts at: 1943 3127 1943
Size: 1184 5627
XmaI C'CCGG_G
Cuts at: 1870 3414 3781 3991 4136 1870
Size: 1544 367 210 145 4545
Fragments arranged by size:
4545 1544 367 210 145
XmnI GAAnn'nnTTC
Cuts at: 2507 4940 2507
Size: 2433 4378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3693 3738 4619 276
Size: 53 83 186 936 2159 45 881 2468
Fragments arranged by size:
2468 2159 936 881 186 83 53 45
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoRV FspAI NruI NsiI PacI PsrI
RsrII SacI SapI SexAI SfiI SrfI SwaI