Restriction map for
pFN21AA1282
(Circular) (Six-base) MAPSORT of: pFN21AA1282.seq Check: 5142 from: 1 to: 7954
pFN21AA1282 7954 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2553 3729 3955 4666 2553
Size: 1176 226 711 5841
Fragments arranged by size:
5841 1176 711 226
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5764 278
Size: 53 83 186 936 4228 2468
Fragments arranged by size:
4228 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2133 5297 1937
Size: 196 3164 4594
Fragments arranged by size:
4594 3164 196
Acc65I G'GTAC_C
Cuts at: 5275 5275
Size: 7954
AclI AA'CG_TT
Cuts at: 1563 6081 6454 1563
Size: 4518 373 3063
Fragments arranged by size:
4518 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3026 4063 6017 7401 7669 1632
Size: 1394 1037 1954 1384 268 1917
Fragments arranged by size:
1954 1917 1394 1384 1037 268
AfeI AGC'GCT
Cuts at: 2946 2946
Size: 7954
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7757
AflIII A'CryG_T
Cuts at: 1203 2982 3414 6853 7830 1203
Size: 1779 432 3439 977 1327
Fragments arranged by size:
3439 1779 1327 977 432
AgeI A'CCGG_T
Cuts at: 3054 6785 3054
Size: 3731 4223
AhdI GACnn_n'nnGTC
Cuts at: 6683 6683
Size: 7954
AleI CACnn'nnGTG
Cuts at: 1166 2855 3398 1166
Size: 1689 543 5722
Fragments arranged by size:
5722 1689 543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7954
AlwNI CAG_nnn'CTG
Cuts at: 2793 3208 4021 4681 4816 5099 5697 7269 2793
Size: 415 813 660 135 283 598 1572 3478
Fragments arranged by size:
3478 1572 813 660 598 415 283 135
ApaI G_GGCC'C
Cuts at: 2429 2998 4220 4414 4611 5091 2429
Size: 569 1222 194 197 480 5292
Fragments arranged by size:
5292 1222 569 480 197 194
ApaLI G'TGCA_C
Cuts at: 6011 7167 6011
Size: 1156 6798
ApoI r'AATT_y
Cuts at: 1493 2839 5263 5489 5641 7750 1493
Size: 1346 2424 226 152 2109 1697
Fragments arranged by size:
2424 2109 1697 1346 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6508 160
Size: 872 5476 1606
Fragments arranged by size:
5476 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 4061 4761 4968 5279 1870
Size: 73 229 1889 700 207 311 4545
Fragments arranged by size:
4545 1889 700 311 229 207 73
AvrII C'CTAG_G
Cuts at: 3919 3919
Size: 7954
BamHI G'GATC_C
Cuts at: 5284 5651 5284
Size: 367 7587
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2460 2541 3470 3902
Size: 325 217 627 242 431 81 929 432
Cuts at: 3902 4735 4921 5275 6730 618
Size: 833 186 354 1455 1842
Fragments arranged by size:
1842 1455 929 833 627 432 431 354
325 242 217 186 81
BanII G_rGCy'C
Cuts at: 1391 2121 2238 2319 2429 2766 2998 4104 4220
Size: 730 117 81 110 337 232 1106 116
Cuts at: 4220 4251 4414 4469 4611 4958 5010 5040 5091
Size: 31 163 55 142 347 52 30 51
Cuts at: 5091 5136 5273 1391
Size: 45 137 4072
Fragments arranged by size:
4072 1106 730 347 337 232 163 142
137 117 116 110 81 55 52 51
45 31 30
BbeI G_GCGC'C
Cuts at: 2033 2464 3474 4739 2033
Size: 431 1010 1265 5248
Fragments arranged by size:
5248 1265 1010 431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3014 4497 928
Size: 971 1115 1483 4385
Fragments arranged by size:
4385 1483 1115 971
BbvCI CC'TCA_GC
Cuts at: 2178 4291 4383 5112 2178
Size: 2113 92 729 5020
Fragments arranged by size:
5020 2113 729 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3892 3926 4031 4065 4691
Size: 34 50 34 1871 34 105 34 626
Cuts at: 4691 4725 5233 5267 6145 6179 1903
Size: 34 508 34 878 34 3678
Fragments arranged by size:
3678 1871 878 626 508 105 50 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 5846 7056 3602
Size: 2244 1210 4500
Fragments arranged by size:
4500 2244 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7954
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2111 2469
Size: 107 122 71 631 605 337 102 358
Cuts at: 2469 3641 3854 4542 6565 136
Size: 1172 213 688 2023 1525
Fragments arranged by size:
2023 1525 1172 688 631 605 358 337
213 122 107 102 71
BglII A'GATC_T
Cuts at: 3441 4459 7949 3441
Size: 1018 3490 3446
Fragments arranged by size:
3490 3446 1018
BlpI GC'TnA_GC
Cuts at: 2289 4103 5371 2289
Size: 1814 1268 4872
Fragments arranged by size:
4872 1814 1268
Bme1580I G_kGCm'C
Cuts at: 1790 2429 2479 2544 2671 2733 2998 3784 4220
Size: 639 50 65 127 62 265 786 436
Cuts at: 4220 4414 4455 4611 4760 4787 5091 6015 7171
Size: 194 41 156 149 27 304 924 1156
Cuts at: 7171 1790
Size: 2573
Fragments arranged by size:
2573 1156 924 786 639 436 304 265
194 156 149 127 65 62 50 41
27
BmgBI CAC'GTC
Cuts at: 1318 3988 4316 1318
Size: 2670 328 4956
Fragments arranged by size:
4956 2670 328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 3130 4839
Size: 416 53 83 399 24 1343 370 1709
Cuts at: 4839 5094 5685 6643 442
Size: 255 591 958 1753
Fragments arranged by size:
1753 1709 1343 958 591 416 399 370
255 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7954
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4118
Size: 7954
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3242 3279 3344 3641 4208 4237 5148 5204
Size: 2003 37 65 297 567 29 911 56
Cuts at: 5204 6614 1239
Size: 1410 2579
Fragments arranged by size:
2579 2003 1410 911 567 297 65 56
37 29
Bpu10I CC'TnA_GC
Cuts at: 2178 4157 4291 4383 5112 2178
Size: 1979 134 92 729 5020
Fragments arranged by size:
5020 1979 729 134 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3590 3878 4894 6079 6944 7242 3590
Size: 288 1016 1185 865 298 4302
Fragments arranged by size:
4302 1185 1016 865 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4231 5016 6617 882
Size: 617 2732 785 1601 2219
Fragments arranged by size:
2732 2219 1601 785 617
BsaAI yAC'GTr
Cuts at: 493 1204 5745 493
Size: 711 4541 2702
Fragments arranged by size:
4541 2702 711
BsaBI GATnn'nnATC
Cuts at: 7948 7948
Size: 7954
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2461 3471 4736
Size: 53 83 186 936 497 431 1010 1265
Cuts at: 4736 5761 6143 6778 275
Size: 1025 382 635 1451
Fragments arranged by size:
1451 1265 1025 1010 936 635 497 431
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2304 2634 3054 6387 6785 7059 7206 7617 2304
Size: 330 420 3333 398 274 147 411 2641
Fragments arranged by size:
3333 2641 420 411 398 330 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3350 3380 4136 4166 4739
Size: 30 165 30 1947 30 756 30 573
Cuts at: 4739 4769 1178
Size: 30 4363
Fragments arranged by size:
4363 1947 756 573 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4205 4273 4899 5049 1186
Size: 3019 68 626 150 4091
Fragments arranged by size:
4091 3019 626 150 68
BseYI C'CCAG_C
Cuts at: 2095 2786 3331 4121 4231 4237 4610 4786 4858
Size: 691 545 790 110 6 373 176 72
Cuts at: 4858 4942 5039 7157 7653 2095
Size: 84 97 2118 496 2396
Fragments arranged by size:
2396 2118 790 691 545 496 373 176
110 97 84 72 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3439 3707 3796 4022 4261 4742 1163
Size: 573 1703 268 89 226 239 481 4375
Fragments arranged by size:
4375 1703 573 481 268 239 226 89
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5404 6165 7193 1540
Size: 275 186 3403 761 1028 2301
Fragments arranged by size:
3403 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2274 2526 2753 3894 3912 4104 4958 6015
Size: 36 252 227 1141 18 192 854 1057
Cuts at: 6015 6100 7171 2238
Size: 85 1071 3021
Fragments arranged by size:
3021 1141 1071 1057 854 252 227 192
85 36 18
BsmI GAATG_Cn'
Cuts at: 5465 5558 5465
Size: 93 7861
BsmBI CGTCTCn'nnnn_
Cuts at: 4953 4953
Size: 7954
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2238 2274 2319 2429 2479 2526
Size: 399 331 117 36 45 110 50 47
Cuts at: 2526 2544 2671 2733 2753 2766 2998 3784 3894
Size: 18 127 62 20 13 232 786 110
Cuts at: 3894 3912 4104 4220 4251 4414 4455 4469 4611
Size: 18 192 116 31 163 41 14 142
Cuts at: 4611 4760 4787 4958 5010 5040 5091 5136 5273
Size: 149 27 171 52 30 51 45 137
Cuts at: 5273 6015 6100 7171 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 786 742 399 331 232 192
171 163 149 142 137 127 117 116
110 110 85 62 52 51 50 47
45 45 41 36 31 30 27 20
18 18 14 13
BspEI T'CCGG_A
Cuts at: 2634 7617 2634
Size: 4983 2971
BspHI T'CATG_A
Cuts at: 5843 7573 5843
Size: 1730 6224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2553 2967 3573 3729 3955 4666 5309 844
Size: 1709 414 606 156 226 711 643 3489
Fragments arranged by size:
3489 1709 711 643 606 414 226 156
BsrBI CCG'CTC
Cuts at: 1394 1823 2313 4161 4584 5841 1394
Size: 429 490 1848 423 1257 3507
Fragments arranged by size:
3507 1848 1257 490 429 423
BsrDI GCAATG_nn'
Cuts at: 65 1248 6449 6623 65
Size: 1183 5201 174 1396
Fragments arranged by size:
5201 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2457 2502 3054 6598 6785 1700
Size: 110 198 449 45 552 3544 187 2869
Fragments arranged by size:
3544 2869 552 449 198 187 110 45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7954
BssHII G'CGCG_C
Cuts at: 1925 3700 1925
Size: 1775 6179
BssSI C'ACGA_G
Cuts at: 6014 7026 6014
Size: 1012 6942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3616 4021 4681 794
Size: 2822 405 660 4067
Fragments arranged by size:
4067 2822 660 405
BstBI TT'CG_AA
Cuts at: 6758 6758
Size: 7954
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7954
BstXI CCAn_nnnn'nTGG
Cuts at: 5213 5213
Size: 7954
BstYI r'GATC_y
Cuts at: 1972 2250 3441 4459 5284 5651 6036 6053 7505
Size: 278 1191 1018 825 367 385 17 1452
Cuts at: 7505 7591 7603 7949 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1191 1018 825 385 367 346
278 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5017 5017
Size: 7954
BtgI C'CryG_G
Cuts at: 513 1065 2127 2776 2805 3044 3075 3766 3882
Size: 552 1062 649 29 239 31 691 116
Cuts at: 3882 4221 4406 4442 4603 5206 513
Size: 339 185 36 161 603 3261
Fragments arranged by size:
3261 1062 691 649 603 552 339 239
185 161 116 36 31 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2041 2923 3694 3858 487
Size: 683 56 815 882 771 164 4583
Fragments arranged by size:
4583 882 815 771 683 164 56
BtsI GCAGTG_nn'
Cuts at: 3086 4022 5470 6264 6284 3086
Size: 936 1448 794 20 4756
Fragments arranged by size:
4756 1448 936 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7954
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7919
DraI TTT'AAA
Cuts at: 5258 5258
Size: 7954
DraIII CAC_nnn'GTG
Cuts at: 3487 3487
Size: 7954
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 6961 809
Size: 507 3350 2295 1802
Fragments arranged by size:
3350 2295 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2455 2470
Size: 54 1425 50 275 18 180 445 15
Cuts at: 2470 2802 3096 3148 3274 3507 3759 5401 6290
Size: 332 294 52 126 233 252 1642 889
Cuts at: 6290 7919 8
Size: 1629 43
Fragments arranged by size:
1642 1629 1425 889 445 332 294 275
252 233 180 126 54 52 50 43
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 5401 1537
Size: 275 3589 4090
Fragments arranged by size:
4090 3589 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2303 2384 2822 5884 1353
Size: 773 177 81 438 3062 3423
Fragments arranged by size:
3423 3062 773 438 177 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2231 2524 3409 6541 6915 7061
Size: 1064 138 642 293 885 3132 374 146
Cuts at: 7061 387
Size: 1280
Fragments arranged by size:
3132 1280 1064 885 642 374 293 146
138
EcoICRI GAG'CTC
Cuts at: 2236 4102 4956 2236
Size: 1866 854 5234
Fragments arranged by size:
5234 1866 854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3026 3242 3279 3344 3641 4063 4208
Size: 393 1394 216 37 65 297 422 145
Cuts at: 4208 4237 5148 5204 6017 6614 7401 7669 1239
Size: 29 911 56 813 597 787 268 1524
Fragments arranged by size:
1524 1394 911 813 787 597 422 393
297 268 216 145 65 56 37 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3725 3923 4338 5016 3725
Size: 198 415 678 6663
Fragments arranged by size:
6663 678 415 198
EcoO109I rG'GnC_Cy
Cuts at: 2020 2260 2425 2426 2812 2994 3733 3856 4216
Size: 240 165 1 386 182 739 123 360
Cuts at: 4216 4217 4411 4904 4937 5152 5170 2020
Size: 1 194 493 33 215 18 4804
Fragments arranged by size:
4804 739 493 386 360 240 215 194
182 165 123 33 18 1 1
EcoRI G'AATT_C
Cuts at: 5263 5641 5263
Size: 378 7576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 7954
FspI TGC'GCA
Cuts at: 3350 6460 3350
Size: 3110 4844
HaeII r_GCGC'y
Cuts at: 1678 2033 2464 2948 3075 3357 3474 4739 7101
Size: 355 431 484 127 282 117 1265 2362
Cuts at: 7101 1678
Size: 2531
Fragments arranged by size:
2531 2362 1265 484 431 355 282 127
117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3044 3076 4136 4168 4235
Size: 32 135 32 1472 32 1060 32 67
Cuts at: 4235 4267 5015 5047 6595 6627 6669 6701 1373
Size: 32 748 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1548 1472 1060 748 135 67 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2980 5298 5544 669
Size: 988 281 1042 2318 246 3079
Fragments arranged by size:
3079 2318 1042 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4643 748
Size: 3895 4059
HpaI GTT'AAC
Cuts at: 5544 5544
Size: 7954
KasI G'GCGC_C
Cuts at: 2029 2460 3470 4735 2029
Size: 431 1010 1265 5248
Fragments arranged by size:
5248 1265 1010 431
KpnI G_GTAC'C
Cuts at: 5279 5279
Size: 7954
MfeI C'AATT_G
Cuts at: 5553 5553
Size: 7954
MluI A'CGCG_T
Cuts at: 7830 7830
Size: 7954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7068 7252 1305
Size: 5763 184 2007
Fragments arranged by size:
5763 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3150 10
Size: 54 1425 343 1318 4814
Fragments arranged by size:
4814 1425 1318 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 3098 3398 3713 3887 5912 6271
Size: 648 1689 243 300 315 174 2025 359
Cuts at: 6271 6430 518
Size: 159 2042
Fragments arranged by size:
2042 2025 1689 648 359 315 300 243
174 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2778 3046 3154 3257 3742 3768 4027
Size: 585 587 268 108 103 485 26 259
Cuts at: 4027 4241 4295 4862 5370 6047 7195 7440 1606
Size: 214 54 567 508 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 587 585 567 508 485
268 259 245 214 108 103 54 26
NaeI GCC'GGC
Cuts at: 2010 2459 2010
Size: 449 7505
NarI GG'CG_CC
Cuts at: 2030 2461 3471 4736 2030
Size: 431 1010 1265 5248
Fragments arranged by size:
5248 1265 1010 431
NcoI C'CATG_G
Cuts at: 513 1065 3882 4442 4603 5206 513
Size: 552 2817 560 161 603 3261
Fragments arranged by size:
3261 2817 603 560 552 161
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7954
NgoMIV G'CCGG_C
Cuts at: 2008 2457 2008
Size: 449 7505
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7954
NotI GC'GGCC_GC
Cuts at: 5401 5401
Size: 7954
NspI r_CATG'y
Cuts at: 1132 1229 2969 3526 3619 5312 6857 1132
Size: 97 1740 557 93 1693 1545 2229
Fragments arranged by size:
2229 1740 1693 1545 557 97 93
PasI CC'CwG_GG
Cuts at: 1348 4470 4932 5166 1348
Size: 3122 462 234 4136
Fragments arranged by size:
4136 3122 462 234
PciI A'CATG_T
Cuts at: 6853 6853
Size: 7954
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4443 5182 1108
Size: 3335 739 3880
Fragments arranged by size:
3880 3335 739
PfoI T'CCnGG_A
Cuts at: 2046 2334 2046
Size: 288 7666
PmeI GTTT'AAAC
Cuts at: 5258 5258
Size: 7954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6023 6055 7536 7568 6023
Size: 32 1481 32 6409
Fragments arranged by size:
6409 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2812 3733 4937 5170 2812
Size: 921 1204 233 5596
Fragments arranged by size:
5596 1204 921 233
PshAI GACnn'nnGTC
Cuts at: 1613 4702 1613
Size: 3089 4865
PsiI TTA'TAA
Cuts at: 5524 5524
Size: 7954
PspOMI G'GGCC_C
Cuts at: 2425 2994 4216 4410 4607 5087 2425
Size: 569 1222 194 197 480 5292
Fragments arranged by size:
5292 1222 569 480 197 194
PspXI vC'TCGA_Gb
Cuts at: 4968 4968
Size: 7954
PstI C_TGCA'G
Cuts at: 830 2550 3204 3293 3681 3741 3855 4017 4026
Size: 1720 654 89 388 60 114 162 9
Cuts at: 4026 4138 4686 4704 4812 4854 5306 830
Size: 112 548 18 108 42 452 3478
Fragments arranged by size:
3478 1720 654 548 452 388 162 114
112 108 89 60 42 18 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7954
PvuII CAG'CTG
Cuts at: 3257 3742 4027 4241 4295 4862 3257
Size: 485 285 214 54 567 6349
Fragments arranged by size:
6349 567 485 285 214 54
SacI G_AGCT'C
Cuts at: 2238 4104 4958 2238
Size: 1866 854 5234
Fragments arranged by size:
5234 1866 854
SacII CC_GC'GG
Cuts at: 2779 3047 3769 2779
Size: 268 722 6964
Fragments arranged by size:
6964 722 268
SalI G'TCGA_C
Cuts at: 1936 5296 1936
Size: 3360 4594
SanDI GG'GwC_CC
Cuts at: 4937 5170 4937
Size: 233 7721
SapI GCTCTTCn'nnn_
Cuts at: 2126 2303 2126
Size: 177 7777
SbfI CC_TGCA'GG
Cuts at: 3855 5306 3855
Size: 1451 6503
ScaI AGT'ACT
Cuts at: 3626 6202 7914 3626
Size: 2576 1712 3666
Fragments arranged by size:
3666 2576 1712
SexAI A'CCwGG_T
Cuts at: 2918 3237 2918
Size: 319 7635
SfcI C'TryA_G
Cuts at: 826 1045 2546 2734 3200 3289 3677 3737 3851
Size: 219 1501 188 466 89 388 60 114
Cuts at: 3851 4013 4022 4134 4682 4700 4808 4850 5302
Size: 162 9 112 548 18 108 42 452
Cuts at: 5302 6437 7118 7309 826
Size: 1135 681 191 1471
Fragments arranged by size:
1501 1471 1135 681 548 466 452 388
219 191 188 162 114 112 108 89
60 42 18 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4542 4542
Size: 7954
SfoI GGC'GCC
Cuts at: 2031 2462 3472 4737 2031
Size: 431 1010 1265 5248
Fragments arranged by size:
5248 1265 1010 431
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7954
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7954
SmaI CCC'GGG
Cuts at: 1872 2174 5281 1872
Size: 302 3107 4545
Fragments arranged by size:
4545 3107 302
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3605 3893 4873 4968 6058 6959
Size: 197 926 1662 288 980 95 1090 901
Cuts at: 6959 7221 820
Size: 262 1553
Fragments arranged by size:
1662 1553 1090 980 926 901 288 262
197 95
SnaBI TAC'GTA
Cuts at: 493 5745 493
Size: 5252 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7954
SphI G_CATG'C
Cuts at: 1132 3619 5312 1132
Size: 2487 1693 3774
Fragments arranged by size:
3774 2487 1693
SspI AAT'ATT
Cuts at: 5 52 5878 5
Size: 47 5826 2081
Fragments arranged by size:
5826 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7954
StyI C'CwwG_G
Cuts at: 513 1065 2559 3838 3882 3919 4442 4603 5206
Size: 552 1494 1279 44 37 523 161 603
Cuts at: 5206 5393 513
Size: 187 3074
Fragments arranged by size:
3074 1494 1279 603 552 523 187 161
44 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 5981 5998 6151 6336 6781 325
Size: 1255 2526 1875 17 153 185 445 1498
Fragments arranged by size:
2526 1875 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 2985 3066
Size: 274 80 33 51 1443 639 369 81
Cuts at: 3066 3624 6200 7912 96
Size: 558 2576 1712 138
Fragments arranged by size:
2576 1712 1443 639 558 369 274 138
81 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4739 4771 7914 7946 4739
Size: 32 3143 32 4747
Fragments arranged by size:
4747 3143 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7954
XbaI T'CTAG_A
Cuts at: 5290 5290
Size: 7954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2713 2713
Size: 7954
XhoI C'TCGA_G
Cuts at: 1943 4968 1943
Size: 3025 4929
XmaI C'CCGG_G
Cuts at: 1870 2172 5279 1870
Size: 302 3107 4545
Fragments arranged by size:
4545 3107 302
XmnI GAAnn'nnTTC
Cuts at: 2042 3443 6083 2042
Size: 1401 2640 3913
Fragments arranged by size:
3913 2640 1401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5762 276
Size: 53 83 186 936 4228 2468
Fragments arranged by size:
4228 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SrfI
SwaI