Restriction map for
pFN21AA1275
(Circular) (Six-base) MAPSORT of: pFN21AA1275.seq Check: 6609 from: 1 to: 8236
pFN21AA1275 8236 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3878 4513 3878
Size: 635 7601
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4409 6046 278
Size: 53 83 186 936 2873 1637 2468
Fragments arranged by size:
2873 2468 1637 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2544 3699 5042 5579 1937
Size: 607 1155 1343 537 4594
Fragments arranged by size:
4594 1343 1155 607 537
Acc65I G'GTAC_C
Cuts at: 5557 5557
Size: 8236
AclI AA'CG_TT
Cuts at: 1563 4881 6363 6736 1563
Size: 3318 1482 373 3063
Fragments arranged by size:
3318 3063 1482 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 3941 4821 5232 6299 7683 7951 1632
Size: 695 1614 880 411 1067 1384 268 1917
Fragments arranged by size:
1917 1614 1384 1067 880 695 411 268
AfeI AGC'GCT
Cuts at: 4346 4346
Size: 8236
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8039
AflIII A'CryG_T
Cuts at: 1203 7135 8112 1203
Size: 5932 977 1327
Fragments arranged by size:
5932 1327 977
AgeI A'CCGG_T
Cuts at: 5401 7067 5401
Size: 1666 6570
AhdI GACnn_n'nnGTC
Cuts at: 6965 6965
Size: 8236
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2409 4083 1184
Size: 1225 1674 5337
Fragments arranged by size:
5337 1674 1225
AlwNI CAG_nnn'CTG
Cuts at: 2165 2827 4732 5979 7551 2165
Size: 662 1905 1247 1572 2850
Fragments arranged by size:
2850 1905 1572 1247 662
ApaI G_GGCC'C
Cuts at: 4668 4668
Size: 8236
ApaLI G'TGCA_C
Cuts at: 3349 6293 7449 3349
Size: 2944 1156 4136
Fragments arranged by size:
4136 2944 1156
ApoI r'AATT_y
Cuts at: 1493 3598 4001 4675 4805 5202 5545 5771 5923
Size: 2105 403 674 130 397 343 226 152
Cuts at: 5923 8032 1493
Size: 2109 1697
Fragments arranged by size:
2109 2105 1697 674 403 397 343 226
152 130
AseI AT'TA_AT
Cuts at: 160 1032 4205 6790 160
Size: 872 3173 2585 1606
Fragments arranged by size:
3173 2585 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
AvaI C'yCGr_G
Cuts at: 1870 1943 5421 5561 1870
Size: 73 3478 140 4545
Fragments arranged by size:
4545 3478 140 73
BamHI G'GATC_C
Cuts at: 5566 5933 5566
Size: 367 7869
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4689 5557 7012 618
Size: 325 217 627 2902 868 1455 1842
Fragments arranged by size:
2902 1842 1455 868 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3267 3867 4360 4593 4668 5555 1391
Size: 1876 600 493 233 75 887 4072
Fragments arranged by size:
4072 1876 887 600 493 233 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 2315 2883 3974 4979 928
Size: 971 345 71 568 1091 1005 4185
Fragments arranged by size:
4185 1091 1005 971 568 345 71
BbvCI CC'TCA_GC
Cuts at: 2828 2828
Size: 8236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6427 6461 1903
Size: 34 4490 34 3678
Fragments arranged by size:
4490 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4581 6128 7338 4581
Size: 1547 1210 5479
Fragments arranged by size:
5479 1547 1210
BclI T'GATC_A
Cuts at: 1548 3985 1548
Size: 2437 5799
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6847 136
Size: 107 122 71 631 605 5175 1525
Fragments arranged by size:
5175 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2009 4550 8231 2009
Size: 2541 3681 2014
Fragments arranged by size:
3681 2541 2014
BlpI GC'TnA_GC
Cuts at: 5653 5653
Size: 8236
Bme1580I G_kGCm'C
Cuts at: 1790 3353 4668 4692 6297 7453 1790
Size: 1563 1315 24 1605 1156 2573
Fragments arranged by size:
2573 1605 1563 1315 1156 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8236
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3205 4362 4937
Size: 416 53 83 399 24 1788 1157 575
Cuts at: 4937 5425 5967 6925 442
Size: 488 542 958 1753
Fragments arranged by size:
1788 1753 1157 958 575 542 488 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8236
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2952 2952
Size: 8236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2243 2357 2636 3392 4220 4434 4560
Size: 972 32 114 279 756 828 214 126
Cuts at: 4560 4962 5133 5515 6896 1239
Size: 402 171 382 1381 2579
Fragments arranged by size:
2579 1381 972 828 756 402 382 279
214 171 126 114 32
Bpu10I CC'TnA_GC
Cuts at: 2828 2828
Size: 8236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 2879 2922 2987 6361 7226 7524 2486
Size: 393 43 65 3374 865 298 3198
Fragments arranged by size:
3374 3198 865 393 298 65 43
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5226 6899 882
Size: 617 3727 1673 2219
Fragments arranged by size:
3727 2219 1673 617
BsaAI yAC'GTr
Cuts at: 493 1204 6027 493
Size: 711 4823 2702
Fragments arranged by size:
4823 2702 711
BsaBI GATnn'nnATC
Cuts at: 8230 8230
Size: 8236
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4406 6043 6425 7060
Size: 53 83 186 936 2873 1637 382 635
Cuts at: 7060 275
Size: 1451
Fragments arranged by size:
2873 1637 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5401 6669 7067 7341 7488 7899 5401
Size: 1268 398 274 147 411 5738
Fragments arranged by size:
5738 1268 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2415 2445 4628 4658 4689
Size: 30 165 30 1012 30 2183 30 31
Cuts at: 4689 4719 1178
Size: 30 4695
Fragments arranged by size:
4695 2183 1012 165 31 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3047 3203 4064 5344 1186
Size: 1861 156 861 1280 4078
Fragments arranged by size:
4078 1861 1280 861 156
BseYI C'CCAG_C
Cuts at: 3354 5460 7439 7935 3354
Size: 2106 1979 496 3655
Fragments arranged by size:
3655 2106 1979 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 1163
Size: 573 633 7030
Fragments arranged by size:
7030 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5686 6447 7475 1540
Size: 275 186 3685 761 1028 2301
Fragments arranged by size:
3685 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3267 3353 3867 4360 4593 5387 6297 6382 7453
Size: 86 514 493 233 794 910 85 1071
Cuts at: 7453 3267
Size: 4050
Fragments arranged by size:
4050 1071 910 794 514 493 233 86
85
BsmI GAATG_Cn'
Cuts at: 5481 5747 5840 5481
Size: 266 93 7877
Fragments arranged by size:
7877 266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3935 3935
Size: 8236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3267 3353 3867 4360 4593 4668 4692
Size: 399 1477 86 514 493 233 75 24
Cuts at: 4692 5387 5555 6297 6382 7453 1391
Size: 695 168 742 85 1071 2174
Fragments arranged by size:
2174 1477 1071 742 695 514 493 399
233 168 86 85 75 24
BspEI T'CCGG_A
Cuts at: 7899 7899
Size: 8236
BspHI T'CATG_A
Cuts at: 6125 7855 6125
Size: 1730 6506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2619 3408 3878 4513 5591 844
Size: 1775 789 470 635 1078 3489
Fragments arranged by size:
3489 1775 1078 789 635 470
BsrBI CCG'CTC
Cuts at: 1394 1823 2626 6123 1394
Size: 429 803 3497 3507
Fragments arranged by size:
3507 3497 803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2086 6731 6905 65
Size: 1183 838 4645 174 1396
Fragments arranged by size:
4645 1396 1183 838 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2490 5318 5401 6880 7067 1700
Size: 110 680 2828 83 1479 187 2869
Fragments arranged by size:
2869 2828 1479 680 187 110 83
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8236
BssSI C'ACGA_G
Cuts at: 4757 6296 7308 4757
Size: 1539 1012 5685
Fragments arranged by size:
5685 1539 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2512 794
Size: 1718 6518
BstBI TT'CG_AA
Cuts at: 3154 7040 3154
Size: 3886 4350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8236
BstXI CCAn_nnnn'nTGG
Cuts at: 2614 4330 2614
Size: 1716 6520
BstYI r'GATC_y
Cuts at: 1972 2009 2812 4550 5566 5933 6318 6335 7787
Size: 37 803 1738 1016 367 385 17 1452
Cuts at: 7787 7873 7885 8231 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1738 1452 1016 803 385 367 346
86 37 17 12
BstZ17I GTA'TAC
Cuts at: 2545 3700 2545
Size: 1155 7081
Bsu36I CC'TnA_GG
Cuts at: 2158 2328 4837 2158
Size: 170 2509 5557
Fragments arranged by size:
5557 2509 170
BtgI C'CryG_G
Cuts at: 513 1065 4542 4616 5097 513
Size: 552 3477 74 481 3652
Fragments arranged by size:
3652 3477 552 481 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7497
Fragments arranged by size:
7497 683 56
BtsI GCAGTG_nn'
Cuts at: 3784 4767 5752 6546 6566 3784
Size: 983 985 794 20 5454
Fragments arranged by size:
5454 985 983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8201
DraI TTT'AAA
Cuts at: 3366 3669 5540 3366
Size: 303 1871 6062
Fragments arranged by size:
6062 1871 303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7243 809
Size: 507 5927 1802
Fragments arranged by size:
5927 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 5683 6572
Size: 54 1425 50 275 18 687 3166 889
Cuts at: 6572 8201 8
Size: 1629 43
Fragments arranged by size:
3166 1629 1425 889 687 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5683 1537
Size: 275 3871 4090
Fragments arranged by size:
4090 3871 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2835 2943 3036 3287 3564 3791 4984
Size: 761 721 108 93 251 277 227 1193
Cuts at: 4984 6166 1353
Size: 1182 3423
Fragments arranged by size:
3423 1193 1182 761 721 277 251 227
108 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6823 7197 7343 387
Size: 1064 138 5234 374 146 1280
Fragments arranged by size:
5234 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3265 3865 4358 4591 3265
Size: 600 493 233 6910
Fragments arranged by size:
6910 600 493 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2243 2327 2357 2636 3392 3941
Size: 393 579 32 84 30 279 756 549
Cuts at: 3941 4220 4434 4560 4821 4962 5133 5232 5515
Size: 279 214 126 261 141 171 99 283
Cuts at: 5515 6299 6896 7683 7951 1239
Size: 784 597 787 268 1524
Fragments arranged by size:
1524 787 784 756 597 579 549 393
283 279 279 268 261 214 171 141
126 99 84 32 30
EcoNI CCTnn'n_nnAGG
Cuts at: 4836 4836
Size: 8236
EcoO109I rG'GnC_Cy
Cuts at: 2380 4664 4665 2380
Size: 2284 1 5951
Fragments arranged by size:
5951 2284 1
EcoRI G'AATT_C
Cuts at: 5545 5923 5545
Size: 378 7858
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3512 4045 4241 3512
Size: 533 196 7507
Fragments arranged by size:
7507 533 196
FspI TGC'GCA
Cuts at: 6742 6742
Size: 8236
HaeII r_GCGC'y
Cuts at: 1678 4348 7383 1678
Size: 2670 3035 2531
Fragments arranged by size:
3035 2670 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2415 2447 4689 4721 5094
Size: 32 135 32 843 32 2242 32 373
Cuts at: 5094 5126 5441 5473 6877 6909 6951 6983 1373
Size: 32 315 32 1404 32 42 32 2626
Fragments arranged by size:
2626 2242 1404 843 373 315 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3784 4410 5580 5826 669
Size: 988 281 1846 626 1170 246 3079
Fragments arranged by size:
3079 1846 1170 988 626 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8236
HpaI GTT'AAC
Cuts at: 5826 5826
Size: 8236
KpnI G_GTAC'C
Cuts at: 5561 5561
Size: 8236
MfeI C'AATT_G
Cuts at: 3938 5835 3938
Size: 1897 6339
MluI A'CGCG_T
Cuts at: 8112 8112
Size: 8236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2272 3958 4119 4311 5168 5305 7350 7534
Size: 967 1686 161 192 857 137 2045 184
Cuts at: 7534 1305
Size: 2007
Fragments arranged by size:
2045 2007 1686 967 857 192 184 161
137
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6414
Fragments arranged by size:
6414 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 2753 5486 6194 6553 6712 518
Size: 648 1225 362 2733 708 359 159 2042
Fragments arranged by size:
2733 2042 1225 708 648 362 359 159
MspA1I CmG'CkG
Cuts at: 1606 2509 3991 4437 4466 5060 5464 5652 6329
Size: 903 1482 446 29 594 404 188 677
Cuts at: 6329 7477 7722 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1482 1148 903 677 594 446 404
245 188 29
NcoI C'CATG_G
Cuts at: 513 1065 4616 5097 513
Size: 552 3551 481 3652
Fragments arranged by size:
3652 3551 552 481
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8236
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8236
NotI GC'GGCC_GC
Cuts at: 5683 5683
Size: 8236
NspI r_CATG'y
Cuts at: 1132 1229 2305 2500 5594 7139 1132
Size: 97 1076 195 3094 1545 2229
Fragments arranged by size:
3094 2229 1545 1076 195 97
PasI CC'CwG_GG
Cuts at: 1348 5240 1348
Size: 3892 4344
PciI A'CATG_T
Cuts at: 7135 7135
Size: 8236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3940 4985 1108
Size: 2832 1045 4359
Fragments arranged by size:
4359 2832 1045
PfoI T'CCnGG_A
Cuts at: 4488 4488
Size: 8236
PmeI GTTT'AAAC
Cuts at: 5540 5540
Size: 8236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6305 6337 7818 7850 6305
Size: 32 1481 32 6691
Fragments arranged by size:
6691 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2380 2380
Size: 8236
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8236
PsiI TTA'TAA
Cuts at: 5806 5806
Size: 8236
PspOMI G'GGCC_C
Cuts at: 4664 4664
Size: 8236
PspXI vC'TCGA_Gb
Cuts at: 5421 5421
Size: 8236
PstI C_TGCA'G
Cuts at: 830 3063 3779 5588 830
Size: 2233 716 1809 3478
Fragments arranged by size:
3478 2233 1809 716
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8236
PvuII CAG'CTG
Cuts at: 2509 3991 4466 5060 2509
Size: 1482 475 594 5685
Fragments arranged by size:
5685 1482 594 475
SacI G_AGCT'C
Cuts at: 3267 3867 4360 4593 3267
Size: 600 493 233 6910
Fragments arranged by size:
6910 600 493 233
SalI G'TCGA_C
Cuts at: 1936 5578 1936
Size: 3642 4594
SbfI CC_TGCA'GG
Cuts at: 5588 5588
Size: 8236
ScaI AGT'ACT
Cuts at: 6484 8196 6484
Size: 1712 6524
SfcI C'TryA_G
Cuts at: 826 1045 3059 3287 3775 4348 5584 6719 7400
Size: 219 2014 228 488 573 1236 1135 681
Cuts at: 7400 7591 826
Size: 191 1471
Fragments arranged by size:
2014 1471 1236 1135 681 573 488 228
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8236
SmaI CCC'GGG
Cuts at: 1872 5563 1872
Size: 3691 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 2894 2937 3002 5421 6340
Size: 197 926 522 429 43 65 2419 919
Cuts at: 6340 7241 7503 820
Size: 901 262 1553
Fragments arranged by size:
2419 1553 926 919 901 522 429 262
197 65 43
SnaBI TAC'GTA
Cuts at: 493 6027 493
Size: 5534 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8236
SphI G_CATG'C
Cuts at: 1132 2500 5594 1132
Size: 1368 3094 3774
Fragments arranged by size:
3774 3094 1368
SspI AAT'ATT
Cuts at: 5 52 6160 5
Size: 47 6108 2081
Fragments arranged by size:
6108 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8236
StyI C'CwwG_G
Cuts at: 513 1065 2020 4616 5097 5675 513
Size: 552 955 2596 481 578 3074
Fragments arranged by size:
3074 2596 955 578 552 481
SwaI ATTT'AAAT
Cuts at: 3366 3366
Size: 8236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3368 4707 5488 6263 6280 6433 6618
Size: 1255 1788 1339 781 775 17 153 185
Cuts at: 6618 7063 325
Size: 445 1498
Fragments arranged by size:
1788 1498 1339 1255 781 775 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2806 4174 4252
Size: 274 80 33 51 1443 829 1368 78
Cuts at: 4252 6482 8194 96
Size: 2230 1712 138
Fragments arranged by size:
2230 1712 1443 1368 829 274 138 80
78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4813 4845 5236 5268 5467 5499 8196 8228 4813
Size: 32 391 32 199 32 2697 32 4821
Fragments arranged by size:
4821 2697 391 199 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5038 1316
Size: 3722 4514
XbaI T'CTAG_A
Cuts at: 4561 5572 4561
Size: 1011 7225
XhoI C'TCGA_G
Cuts at: 1943 5421 1943
Size: 3478 4758
XmaI C'CCGG_G
Cuts at: 1870 5561 1870
Size: 3691 4545
XmnI GAAnn'nnTTC
Cuts at: 3078 3150 4484 4675 6365 3078
Size: 72 1334 191 1690 4949
Fragments arranged by size:
4949 1690 1334 191 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4407 6044 276
Size: 53 83 186 936 2873 1637 2468
Fragments arranged by size:
2873 2468 1637 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI XcmI