Restriction map for
pFN21AA1273
(Circular) (Six-base) MAPSORT of: pFN21AA1273.seq Check: 7694 from: 1 to: 6511
pFN21AA1273 6511 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2732 4321 278
Size: 53 83 186 936 1196 1589 2468
Fragments arranged by size:
2468 1589 1196 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2649 2794 3854 1937
Size: 712 145 1060 4594
Fragments arranged by size:
4594 1060 712 145
Acc65I G'GTAC_C
Cuts at: 3832 3832
Size: 6511
AclI AA'CG_TT
Cuts at: 1563 4638 5011 1563
Size: 3075 373 3063
Fragments arranged by size:
3075 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 2525 3398 3602 4574 5958 6226 1632
Size: 689 204 873 204 972 1384 268 1917
Fragments arranged by size:
1917 1384 972 873 689 268 204 204
AfeI AGC'GCT
Cuts at: 2285 2285
Size: 6511
AflII C'TTAA_G
Cuts at: 820 1017 3347 820
Size: 197 2330 3984
Fragments arranged by size:
3984 2330 197
AflIII A'CryG_T
Cuts at: 1203 5410 6387 1203
Size: 4207 977 1327
Fragments arranged by size:
4207 1327 977
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 5240 5240
Size: 6511
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2759 1184
Size: 1575 4936
AlwNI CAG_nnn'CTG
Cuts at: 4254 5826 4254
Size: 1572 4939
ApaI G_GGCC'C
Cuts at: 2257 2257
Size: 6511
ApaLI G'TGCA_C
Cuts at: 4568 5724 4568
Size: 1156 5355
ApoI r'AATT_y
Cuts at: 1493 2329 3203 3820 4046 4198 6307 1493
Size: 836 874 617 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 836 617 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5065 160
Size: 872 4033 1606
Fragments arranged by size:
4033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 2445 3836 1870
Size: 73 502 1391 4545
Fragments arranged by size:
4545 1391 502 73
BamHI G'GATC_C
Cuts at: 3841 4208 3841
Size: 367 6144
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 2760 2930 3372 3798
Size: 325 217 627 768 205 170 442 426
Cuts at: 3798 3832 5287 618
Size: 34 1455 1842
Fragments arranged by size:
1842 1455 768 627 442 426 325 217
205 170 34
BanII G_rGCy'C
Cuts at: 1391 2257 3830 1391
Size: 866 1573 4072
Fragments arranged by size:
4072 1573 866
BbeI G_GCGC'C
Cuts at: 3376 3376
Size: 6511
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 2945 928
Size: 971 323 723 4494
Fragments arranged by size:
4494 971 723 323
BbvCI CC'TCA_GC
Cuts at: 2202 2202
Size: 6511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4702 4736 1903
Size: 34 50 34 2681 34 3678
Fragments arranged by size:
3678 2681 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4403 5613 4403
Size: 1210 5301
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6511
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2618 3078 3690
Size: 107 122 71 631 605 946 460 612
Cuts at: 3690 5122 136
Size: 1432 1525
Fragments arranged by size:
1525 1432 946 631 612 605 460 122
107 71
BglII A'GATC_T
Cuts at: 6506 6506
Size: 6511
BlpI GC'TnA_GC
Cuts at: 3459 3928 3459
Size: 469 6042
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2257 3740 3801 4572 5728 1790
Size: 260 207 1483 61 771 1156 2573
Fragments arranged by size:
2573 1483 1156 771 260 207 61
BmgBI CAC'GTC
Cuts at: 1318 2806 3007 3271 1318
Size: 1488 201 264 4558
Fragments arranged by size:
4558 1488 264 201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2251 3067 3382
Size: 416 53 83 399 24 834 816 315
Cuts at: 3382 3738 4242 5200 442
Size: 356 504 958 1753
Fragments arranged by size:
1753 958 834 816 504 416 399 356
315 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2768 2831 5171 1239
Size: 1529 63 2340 2579
Fragments arranged by size:
2579 2340 1529 63
Bpu10I CC'TnA_GC
Cuts at: 2112 2202 2222 3234 2112
Size: 90 20 1012 5389
Fragments arranged by size:
5389 1012 90 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 4636 5501 5799 2159
Size: 2477 865 298 2871
Fragments arranged by size:
2871 2477 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 2429 3632 5174 882
Size: 617 726 204 1203 1542 2219
Fragments arranged by size:
2219 1542 1203 726 617 204
BsaAI yAC'GTr
Cuts at: 493 1204 4302 493
Size: 711 3098 2702
Fragments arranged by size:
3098 2702 711
BsaBI GATnn'nnATC
Cuts at: 6505 6505
Size: 6511
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2510 2729 3108 3373
Size: 53 83 186 936 977 219 379 265
Cuts at: 3373 3531 4318 4700 5335 275
Size: 158 787 382 635 1451
Fragments arranged by size:
1451 977 936 787 635 382 379 265
219 186 158 83 53
BsaWI w'CCGG_w
Cuts at: 3623 4944 5342 5616 5763 6174 3623
Size: 1321 398 274 147 411 3960
Fragments arranged by size:
3960 1321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 3425 3455 1178
Size: 30 165 30 675 30 1317 30 4234
Fragments arranged by size:
4234 1317 675 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2224 2243 2339 2360 2777 3149 3317
Size: 1033 5 19 96 21 417 372 168
Cuts at: 3317 1186
Size: 4380
Fragments arranged by size:
4380 1033 417 372 168 96 21 19
5
BseYI C'CCAG_C
Cuts at: 2621 2701 2831 5714 6210 2621
Size: 80 130 2883 496 2922
Fragments arranged by size:
2922 2883 496 130 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2015 2096 2375 2951 3682 1163
Size: 573 279 81 279 576 731 3992
Fragments arranged by size:
3992 731 576 573 279 279 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 3782 3961 4722 5750 1540
Size: 275 186 1777 4 179 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186 179 4
BsiHKAI G_wGCw'C
Cuts at: 2143 2520 3413 3615 4572 4657 5728 2143
Size: 377 893 202 957 85 1071 2926
Fragments arranged by size:
2926 1071 957 893 377 202 85
BsmI GAATG_Cn'
Cuts at: 2479 2668 4022 4115 2479
Size: 189 1354 93 4875
Fragments arranged by size:
4875 1354 189 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2720 2720
Size: 6511
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2143 2257 2520 3413 3615 3740
Size: 399 260 93 114 263 893 202 125
Cuts at: 3740 3801 3830 4572 4657 5728 1391
Size: 61 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 893 742 399 263 260 202
125 114 93 85 61 29
BspEI T'CCGG_A
Cuts at: 3623 6174 3623
Size: 2551 3960
BspHI T'CATG_A
Cuts at: 2991 4400 6130 2991
Size: 1409 1730 3372
Fragments arranged by size:
3372 1730 1409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 3297 3866 844
Size: 1190 1263 569 3489
Fragments arranged by size:
3489 1263 1190 569
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 2392 2467 3307 4398 1394
Size: 429 266 303 75 840 1091 3507
Fragments arranged by size:
3507 1091 840 429 303 266 75
BsrDI GCAATG_nn'
Cuts at: 65 1248 5006 5180 65
Size: 1183 3758 174 1396
Fragments arranged by size:
3758 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2778 3072 3352 3689 5155 5342 1700
Size: 110 968 294 280 337 1466 187 2869
Fragments arranged by size:
2869 1466 968 337 294 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 2898 96
Size: 2802 3709
BssHII G'CGCG_C
Cuts at: 1925 2450 2452 1925
Size: 525 2 5984
Fragments arranged by size:
5984 525 2
BssSI C'ACGA_G
Cuts at: 4571 5583 4571
Size: 1012 5499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2911 794
Size: 2117 4394
BstBI TT'CG_AA
Cuts at: 5315 5315
Size: 6511
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6511
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6511
BstYI r'GATC_y
Cuts at: 1972 2187 2496 3841 4208 4593 4610 6062 6148
Size: 215 309 1345 367 385 17 1452 86
Cuts at: 6148 6160 6506 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1345 385 367 346 309 215
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2163 2385 2613 3015 3483 3663 513
Size: 552 1098 222 228 402 468 180 3361
Fragments arranged by size:
3361 1098 552 468 402 228 222 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2842 487
Size: 683 56 1616 4156
Fragments arranged by size:
4156 1616 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 4027 4821 4841 2107
Size: 1920 794 20 3777
Fragments arranged by size:
3777 1920 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6476
DraI TTT'AAA
Cuts at: 3815 3815
Size: 6511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3275 5518 809
Size: 507 1959 2243 1802
Fragments arranged by size:
2243 1959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2680 3219 3354
Size: 54 1425 50 275 18 850 539 135
Cuts at: 3354 3465 3660 3779 3958 4847 6476 8
Size: 111 195 119 179 889 1629 43
Fragments arranged by size:
1629 1425 889 850 539 275 195 179
135 119 111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3779 3958 1537
Size: 275 1967 179 4090
Fragments arranged by size:
4090 1967 275 179
EarI CTCTTCn'nnn_
Cuts at: 1353 3095 4441 1353
Size: 1742 1346 3423
Fragments arranged by size:
3423 1742 1346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2489 3602 5098 5472 5618 387
Size: 1064 138 900 1113 1496 374 146 1280
Fragments arranged by size:
1496 1280 1113 1064 900 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2525 2768 2831 3398 3602 4574
Size: 393 689 204 243 63 567 204 972
Cuts at: 4574 5171 5958 6226 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 972 787 689 597 567 393 268
243 204 204 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 3515 2225
Size: 1290 5221
EcoO109I rG'GnC_Cy
Cuts at: 2253 2893 3148 3508 3593 3743 2253
Size: 640 255 360 85 150 5021
Fragments arranged by size:
5021 640 360 255 150 85
EcoRI G'AATT_C
Cuts at: 3820 4198 3820
Size: 378 6133
FspI TGC'GCA
Cuts at: 5017 5017
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 2287 2756 3316 3376 5658 1678
Size: 609 469 560 60 2282 2531
Fragments arranged by size:
2531 2282 609 560 469 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 3425 3457 5152
Size: 32 135 32 506 32 1315 32 1695
Cuts at: 5152 5184 5226 5258 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1695 1315 506 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2795 3855 4101 669
Size: 988 281 857 1060 246 3079
Fragments arranged by size:
3079 1060 988 857 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6511
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KasI G'GCGC_C
Cuts at: 3372 3372
Size: 6511
KpnI G_GTAC'C
Cuts at: 3836 3836
Size: 6511
MfeI C'AATT_G
Cuts at: 4110 4110
Size: 6511
MluI A'CGCG_T
Cuts at: 6387 6387
Size: 6511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5625 5809 1305
Size: 4320 184 2007
Fragments arranged by size:
4320 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3221 3662 10
Size: 54 1425 343 1389 441 2859
Fragments arranged by size:
2859 1425 1389 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2415 2741 3269 4469 4828 4987 518
Size: 648 1249 326 528 1200 359 159 2042
Fragments arranged by size:
2042 1249 1200 648 528 359 326 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2126 3463 3927 4604 5752 5997 1606
Size: 405 115 1337 464 677 1148 245 2120
Fragments arranged by size:
2120 1337 1148 677 464 405 245 115
NaeI GCC'GGC
Cuts at: 2780 3074 3354 3691 2780
Size: 294 280 337 5600
Fragments arranged by size:
5600 337 294 280
NarI GG'CG_CC
Cuts at: 3373 3373
Size: 6511
NcoI C'CATG_G
Cuts at: 513 1065 3015 513
Size: 552 1950 4009
Fragments arranged by size:
4009 1950 552
NdeI CA'TA_TG
Cuts at: 387 3490 387
Size: 3103 3408
NgoMIV G'CCGG_C
Cuts at: 2778 3072 3352 3689 2778
Size: 294 280 337 5600
Fragments arranged by size:
5600 337 294 280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6511
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NspI r_CATG'y
Cuts at: 1132 1229 3442 3869 5414 1132
Size: 97 2213 427 1545 2229
Fragments arranged by size:
2229 2213 1545 427 97
PasI CC'CwG_GG
Cuts at: 1348 3740 3794 1348
Size: 2392 54 4065
Fragments arranged by size:
4065 2392 54
PciI A'CATG_T
Cuts at: 5410 5410
Size: 6511
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6511
PfoI T'CCnGG_A
Cuts at: 3716 3716
Size: 6511
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 6093 6125 4580
Size: 32 1481 32 4966
Fragments arranged by size:
4966 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3148 3508 3148
Size: 360 6151
PshAI GACnn'nnGTC
Cuts at: 1613 2672 1613
Size: 1059 5452
PsiI TTA'TAA
Cuts at: 4081 4081
Size: 6511
PspOMI G'GGCC_C
Cuts at: 2253 2253
Size: 6511
PstI C_TGCA'G
Cuts at: 830 2148 3863 830
Size: 1318 1715 3478
Fragments arranged by size:
3478 1715 1318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
PvuII CAG'CTG
Cuts at: 2011 2126 3463 2011
Size: 115 1337 5059
Fragments arranged by size:
5059 1337 115
SalI G'TCGA_C
Cuts at: 1936 2793 3853 1936
Size: 857 1060 4594
Fragments arranged by size:
4594 1060 857
SapI GCTCTTCn'nnn_
Cuts at: 3095 3095
Size: 6511
SbfI CC_TGCA'GG
Cuts at: 3863 3863
Size: 6511
ScaI AGT'ACT
Cuts at: 4759 6471 4759
Size: 1712 4799
SfcI C'TryA_G
Cuts at: 826 1045 2144 3859 4994 5675 5866 826
Size: 219 1099 1715 1135 681 191 1471
Fragments arranged by size:
1715 1471 1135 1099 681 219 191
SfoI GGC'GCC
Cuts at: 3374 3374
Size: 6511
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6511
SmaI CCC'GGG
Cuts at: 1872 2447 3838 1872
Size: 575 1391 4545
Fragments arranged by size:
4545 1391 575
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 3347 4615 5516 5778 820
Size: 197 926 231 1173 1268 901 262 1553
Fragments arranged by size:
1553 1268 1173 926 901 262 231 197
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 2038 152
Size: 1886 4625
SphI G_CATG'C
Cuts at: 1132 3869 1132
Size: 2737 3774
SrfI GCCC'GGGC
Cuts at: 2447 2447
Size: 6511
SspI AAT'ATT
Cuts at: 5 52 4435 5
Size: 47 4383 2081
Fragments arranged by size:
4383 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6511
StyI C'CwwG_G
Cuts at: 513 1065 2457 3015 3498 3631 3950 513
Size: 552 1392 558 483 133 319 3074
Fragments arranged by size:
3074 1392 558 552 483 319 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 4538 4555 4708 4893 5338 325
Size: 1255 873 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 873 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2898 4757 6469
Size: 274 80 33 51 1443 921 1859 1712
Cuts at: 6469 96
Size: 138
Fragments arranged by size:
1859 1712 1443 921 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 3751 3783 6471 6503 3605
Size: 32 114 32 2688 32 3613
Fragments arranged by size:
3613 2688 114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3506 1316
Size: 2190 4321
XbaI T'CTAG_A
Cuts at: 3847 3847
Size: 6511
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6511
XmaI C'CCGG_G
Cuts at: 1870 2445 3836 1870
Size: 575 1391 4545
Fragments arranged by size:
4545 1391 575
XmnI GAAnn'nnTTC
Cuts at: 3170 4640 3170
Size: 1470 5041
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2730 4319 276
Size: 53 83 186 936 1196 1589 2468
Fragments arranged by size:
2468 1589 1196 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SwaI XcmI