Restriction map for
pFN21AA1270
(Circular) (Six-base) MAPSORT of: pFN21AA1270.seq Check: 6848 from: 1 to: 7660
pFN21AA1270 7660 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3921 3921
Size: 7660
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5470 278
Size: 53 83 186 936 3934 2468
Fragments arranged by size:
3934 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3620 5003 1937
Size: 1683 1383 4594
Fragments arranged by size:
4594 1683 1383
Acc65I G'GTAC_C
Cuts at: 3130 4981 3130
Size: 1851 5809
AclI AA'CG_TT
Cuts at: 1563 5787 6160 1563
Size: 4224 373 3063
Fragments arranged by size:
4224 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3273 3327 3705 5723 7107 7375 1632
Size: 1641 54 378 2018 1384 268 1917
Fragments arranged by size:
2018 1917 1641 1384 378 268 54
AfeI AGC'GCT
Cuts at: 3665 3665
Size: 7660
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7463
AflIII A'CryG_T
Cuts at: 1203 2305 6559 7536 1203
Size: 1102 4254 977 1327
Fragments arranged by size:
4254 1327 1102 977
AgeI A'CCGG_T
Cuts at: 6491 6491
Size: 7660
AhdI GACnn_n'nnGTC
Cuts at: 6389 6389
Size: 7660
AleI CACnn'nnGTG
Cuts at: 1166 4736 1166
Size: 3570 4090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4581 4754 1184
Size: 3397 173 4090
Fragments arranged by size:
4090 3397 173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 7628
AlwNI CAG_nnn'CTG
Cuts at: 2779 3003 3940 4163 4390 4624 4670 5403 6975
Size: 224 937 223 227 234 46 733 1572
Cuts at: 6975 2779
Size: 3464
Fragments arranged by size:
3464 1572 937 733 234 227 224 223
46
ApaI G_GGCC'C
Cuts at: 2664 3784 3947 2664
Size: 1120 163 6377
Fragments arranged by size:
6377 1120 163
ApaLI G'TGCA_C
Cuts at: 5717 6873 5717
Size: 1156 6504
ApoI r'AATT_y
Cuts at: 1493 4969 5195 5347 7456 1493
Size: 3476 226 152 2109 1697
Fragments arranged by size:
3476 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6214 160
Size: 872 5182 1606
Fragments arranged by size:
5182 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7660
AvaI C'yCGr_G
Cuts at: 1870 1943 3777 4371 4985 1870
Size: 73 1834 594 614 4545
Fragments arranged by size:
4545 1834 614 594 73
AvrII C'CTAG_G
Cuts at: 3118 3118
Size: 7660
BamHI G'GATC_C
Cuts at: 2275 2487 3060 4990 5357 2275
Size: 212 573 1930 367 4578
Fragments arranged by size:
4578 1930 573 367 212
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2267 2587 2908 3103
Size: 325 217 627 392 88 320 321 195
Cuts at: 3103 3130 3590 4099 4186 4799 4892 4981 6436
Size: 27 460 509 87 613 93 89 1455
Cuts at: 6436 618
Size: 1842
Fragments arranged by size:
1842 1455 627 613 509 460 392 325
321 320 217 195 93 89 88 87
27
BanII G_rGCy'C
Cuts at: 1391 2106 2664 2707 3784 3947 3968 4874 4979
Size: 715 558 43 1077 163 21 906 105
Cuts at: 4979 1391
Size: 4072
Fragments arranged by size:
4072 1077 906 715 558 163 105 43
21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2728 3013 928
Size: 971 829 285 5575
Fragments arranged by size:
5575 971 829 285
BbvCI CC'TCA_GC
Cuts at: 2078 4949 2078
Size: 2871 4789
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5851 5885 1903
Size: 34 3914 34 3678
Fragments arranged by size:
3914 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2638 5552 6762 2638
Size: 2914 1210 3536
Fragments arranged by size:
3536 2914 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7660
BfrBI ATG'CAT
Cuts at: 4202 4202
Size: 7660
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2068 2132 2788
Size: 107 122 71 631 605 396 64 656
Cuts at: 2788 3230 4367 4519 6271 136
Size: 442 1137 152 1752 1525
Fragments arranged by size:
1752 1525 1137 656 631 605 442 396
152 122 107 71 64
BglII A'GATC_T
Cuts at: 3093 7655 3093
Size: 4562 3098
BlpI GC'TnA_GC
Cuts at: 2576 3456 5077 2576
Size: 880 1621 5159
Fragments arranged by size:
5159 1621 880
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2270 2664 2911 3448 3528 3784 3928
Size: 394 86 394 247 537 80 256 144
Cuts at: 3928 3947 4104 4191 4328 4834 5721 6877 1790
Size: 19 157 87 137 506 887 1156 2573
Fragments arranged by size:
2573 1156 887 537 506 394 394 256
247 157 144 137 87 86 80 19
BmgBI CAC'GTC
Cuts at: 1318 2306 3760 4734 1318
Size: 988 1454 974 4244
Fragments arranged by size:
4244 1454 988 974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2208 3161 3764
Size: 416 53 83 399 24 791 953 603
Cuts at: 3764 3931 4284 4359 4510 5391 6349 442
Size: 167 353 75 151 881 958 1753
Fragments arranged by size:
1753 958 953 881 791 603 416 399
353 167 151 83 75 53 24
BmtI G_CTAG'C
Cuts at: 1056 2598 1056
Size: 1542 6118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4654 4699 4654
Size: 45 7615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2358 2576 3260 3395 3431 4649 6320 1239
Size: 1119 218 684 135 36 1218 1671 2579
Fragments arranged by size:
2579 1671 1218 1119 684 218 135 36
Bpu10I CC'TnA_GC
Cuts at: 2078 2581 3981 4949 2078
Size: 503 1400 968 4789
Fragments arranged by size:
4789 1400 968 503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5785 6650 6948 5785
Size: 865 298 6497
Fragments arranged by size:
6497 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2367 2552 3330 6323 882
Size: 617 868 185 778 2993 2219
Fragments arranged by size:
2993 2219 868 778 617 185
BsaAI yAC'GTr
Cuts at: 493 1204 4271 5451 493
Size: 711 3067 1180 2702
Fragments arranged by size:
3067 2702 1180 711
BsaBI GATnn'nnATC
Cuts at: 3162 7654 3162
Size: 4492 3168
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3018 3877 5467 5849
Size: 53 83 186 936 1485 859 1590 382
Cuts at: 5849 6484 275
Size: 635 1451
Fragments arranged by size:
1590 1485 1451 936 859 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4895 6093 6491 6765 6912 7323 4895
Size: 1198 398 274 147 411 5232
Fragments arranged by size:
5232 1198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3542 3572 4199 4229 1178
Size: 30 165 30 2139 30 627 30 4609
Fragments arranged by size:
4609 2139 627 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 3392 3443 3665 4109 1186
Size: 1270 936 51 222 444 4737
Fragments arranged by size:
4737 1270 936 444 222 51
BseYI C'CCAG_C
Cuts at: 2709 2784 3435 3569 4210 4362 4727 4755 6863
Size: 75 651 134 641 152 365 28 2108
Cuts at: 6863 7359 2709
Size: 496 3010
Fragments arranged by size:
3010 2108 651 641 496 365 152 134
75 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3872 4076 4864 1163
Size: 573 2136 204 788 3959
Fragments arranged by size:
3959 2136 788 573 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2090 2127 5110 5871 6899 1540
Size: 275 186 89 37 2983 761 1028 2301
Fragments arranged by size:
2983 2301 1028 761 275 186 89 37
BsiHKAI G_wGCw'C
Cuts at: 4743 5721 5806 6877 4743
Size: 978 85 1071 5526
Fragments arranged by size:
5526 1071 978 85
BsmI GAATG_Cn'
Cuts at: 5171 5264 5171
Size: 93 7567
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2184 2270 2664 2707 2911 3448
Size: 399 316 78 86 394 43 204 537
Cuts at: 3448 3528 3784 3928 3947 3968 4104 4191 4328
Size: 80 256 144 19 21 136 87 137
Cuts at: 4328 4743 4834 4874 4979 5721 5806 6877 1391
Size: 415 91 40 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 537 415 399 394 316
256 204 144 137 136 105 91 87
86 85 80 78 43 40 21 19
BspEI T'CCGG_A
Cuts at: 7323 7323
Size: 7660
BspHI T'CATG_A
Cuts at: 5549 7279 5549
Size: 1730 5930
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3921 5015 844
Size: 3077 1094 3489
Fragments arranged by size:
3489 3077 1094
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 3244 3415 4420 4633 5547 1394
Size: 429 269 1152 171 1005 213 914 3507
Fragments arranged by size:
3507 1152 1005 914 429 269 213 171
BsrDI GCAATG_nn'
Cuts at: 65 1248 6155 6329 65
Size: 1183 4907 174 1396
Fragments arranged by size:
4907 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2172 6304 6491 1700
Size: 110 362 4132 187 2869
Fragments arranged by size:
4132 2869 362 187 110
BsrGI T'GTAC_A
Cuts at: 96 4086 96
Size: 3990 3670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7660
BssSI C'ACGA_G
Cuts at: 5720 6732 5720
Size: 1012 6648
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2779 794
Size: 1985 5675
BstBI TT'CG_AA
Cuts at: 2053 6464 2053
Size: 4411 3249
BstEII G'GTnAC_C
Cuts at: 1184 2525 1184
Size: 1341 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 3500 3668 3791 4127 3500
Size: 168 123 336 7033
Fragments arranged by size:
7033 336 168 123
BstYI r'GATC_y
Cuts at: 1972 2275 2487 2508 2673 3060 3093 4990 5357
Size: 303 212 21 165 387 33 1897 367
Cuts at: 5357 5742 5759 7211 7297 7309 7655 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1897 1452 387 385 367 346 303
212 165 86 33 21 17 12
BstZ17I GTA'TAC
Cuts at: 3621 3621
Size: 7660
Bsu36I CC'TnA_GG
Cuts at: 2493 2493
Size: 7660
BtgI C'CryG_G
Cuts at: 513 1065 2004 2164 2203 2271 3087 3648 3727
Size: 552 939 160 39 68 816 561 79
Cuts at: 3727 4065 4191 4761 513
Size: 338 126 570 3412
Fragments arranged by size:
3412 939 816 570 561 552 338 160
126 79 68 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6921
Fragments arranged by size:
6921 683 56
BtsI GCAGTG_nn'
Cuts at: 3645 4047 4113 4226 4572 4845 5176 5970 5990
Size: 402 66 113 346 273 331 794 20
Cuts at: 5990 3645
Size: 5315
Fragments arranged by size:
5315 794 402 346 331 273 113 66
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7660
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7625
DraI TTT'AAA
Cuts at: 4964 4964
Size: 7660
DraIII CAC_nnn'GTG
Cuts at: 2999 3332 2999
Size: 333 7327
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6667 809
Size: 507 5351 1802
Fragments arranged by size:
5351 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 2124 2167
Size: 54 1425 50 275 18 257 37 43
Cuts at: 2167 2310 3903 4341 4410 4539 5107 5996 7625
Size: 143 1593 438 69 129 568 889 1629
Cuts at: 7625 8
Size: 43
Fragments arranged by size:
1629 1593 1425 889 568 438 275 257
143 129 69 54 50 43 43 37
18
EagI C'GGCC_G
Cuts at: 1537 1812 2087 2124 5107 1537
Size: 275 275 37 2983 4090
Fragments arranged by size:
4090 2983 275 275 37
EarI CTCTTCn'nnn_
Cuts at: 1353 5590 1353
Size: 4237 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3268 4463 6247 6621 6767 387
Size: 1064 138 1679 1195 1784 374 146 1280
Fragments arranged by size:
1784 1679 1280 1195 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2358 2576 3260 3273 3327 3395 3431
Size: 393 726 218 684 13 54 68 36
Cuts at: 3431 3705 4649 5723 6320 7107 7375 1239
Size: 274 944 1074 597 787 268 1524
Fragments arranged by size:
1524 1074 944 787 726 684 597 393
274 268 218 68 54 36 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3809 4394 3809
Size: 585 7075
EcoO109I rG'GnC_Cy
Cuts at: 2074 2661 3038 3276 3284 3944 4123 4288 4646
Size: 587 377 238 8 660 179 165 358
Cuts at: 4646 4723 2074
Size: 77 5011
Fragments arranged by size:
5011 660 587 377 358 238 179 165
77 8
EcoRI G'AATT_C
Cuts at: 4969 5347 4969
Size: 378 7282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 7660
FspI TGC'GCA
Cuts at: 6166 6166
Size: 7660
HaeII r_GCGC'y
Cuts at: 1678 3517 3667 4112 6807 1678
Size: 1839 150 445 2695 2531
Fragments arranged by size:
2695 2531 1839 445 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3022 3052 3054 3084 3247
Size: 32 135 32 1450 30 2 30 163
Cuts at: 3247 3279 3786 3818 4199 4231 4652 4684 6301
Size: 32 507 32 381 32 421 32 1617
Cuts at: 6301 6333 6375 6407 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1617 1450 507 421 381 163 135
42 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5004 5250 669
Size: 988 281 3066 246 3079
Fragments arranged by size:
3079 3066 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7660
HpaI GTT'AAC
Cuts at: 5250 5250
Size: 7660
KpnI G_GTAC'C
Cuts at: 3134 4985 3134
Size: 1851 5809
MfeI C'AATT_G
Cuts at: 2410 5259 2410
Size: 2849 4811
MluI A'CGCG_T
Cuts at: 7536 7536
Size: 7660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2326 3038 6774 6958 1305
Size: 1021 712 3736 184 2007
Fragments arranged by size:
3736 2007 1021 712 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 2312 4541 10
Size: 54 1425 343 337 143 2229 3129
Fragments arranged by size:
3129 2229 1425 343 337 143 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 3864 4736 5618 5977 6136 518
Size: 648 1638 1060 872 882 359 159 2042
Fragments arranged by size:
2042 1638 1060 882 872 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2205 2713 2788 2878 3046 3404 3573
Size: 418 181 508 75 90 168 358 169
Cuts at: 3573 3856 3988 4525 4561 4711 5076 5753 6901
Size: 283 132 537 36 150 365 677 1148
Cuts at: 6901 7146 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 537 508 418 365 358
283 245 181 169 168 150 132 90
75 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 2164 3087 4761 513
Size: 552 939 160 923 1674 3412
Fragments arranged by size:
3412 1674 939 923 552 160
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7660
NheI G'CTAG_C
Cuts at: 1052 2594 1052
Size: 1542 6118
NotI GC'GGCC_GC
Cuts at: 5107 5107
Size: 7660
NruI TCG'CGA
Cuts at: 4876 4876
Size: 7660
NsiI A_TGCA'T
Cuts at: 4204 4204
Size: 7660
NspI r_CATG'y
Cuts at: 1132 1229 2795 3863 5018 6563 1132
Size: 97 1566 1068 1155 1545 2229
Fragments arranged by size:
2229 1566 1545 1155 1068 97
PasI CC'CwG_GG
Cuts at: 1348 3026 3280 3940 4795 1348
Size: 1678 254 660 855 4213
Fragments arranged by size:
4213 1678 855 660 254
PciI A'CATG_T
Cuts at: 6559 6559
Size: 7660
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 4204 4390 4762 1108
Size: 1068 2028 186 372 4006
Fragments arranged by size:
4006 2028 1068 372 186
PmeI GTTT'AAAC
Cuts at: 4964 4964
Size: 7660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5729 5761 7242 7274 5729
Size: 32 1481 32 6115
Fragments arranged by size:
6115 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3038 3276 4123 4288 3038
Size: 238 847 165 6410
Fragments arranged by size:
6410 847 238 165
PshAI GACnn'nnGTC
Cuts at: 1613 2477 1613
Size: 864 6796
PsiI TTA'TAA
Cuts at: 5230 5230
Size: 7660
PspOMI G'GGCC_C
Cuts at: 2660 3780 3943 2660
Size: 1120 163 6377
Fragments arranged by size:
6377 1120 163
PstI C_TGCA'G
Cuts at: 830 2029 2113 2775 2906 3513 4233 4560 5012
Size: 1199 84 662 131 607 720 327 452
Cuts at: 5012 830
Size: 3478
Fragments arranged by size:
3478 1199 720 662 607 452 327 131
84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7660
PvuII CAG'CTG
Cuts at: 2024 2713 3856 3988 4711 2024
Size: 689 1143 132 723 4973
Fragments arranged by size:
4973 1143 723 689 132
SacII CC_GC'GG
Cuts at: 2206 2206
Size: 7660
SalI G'TCGA_C
Cuts at: 1936 5002 1936
Size: 3066 4594
SbfI CC_TGCA'GG
Cuts at: 5012 5012
Size: 7660
ScaI AGT'ACT
Cuts at: 5908 7620 5908
Size: 1712 5948
SexAI A'CCwGG_T
Cuts at: 2730 3201 3735 3846 4290 2730
Size: 471 534 111 444 6100
Fragments arranged by size:
6100 534 471 444 111
SfcI C'TryA_G
Cuts at: 826 1045 2025 2109 2771 2902 3135 3509 4229
Size: 219 980 84 662 131 233 374 720
Cuts at: 4229 4281 4556 5008 6143 6824 7015 826
Size: 52 275 452 1135 681 191 1471
Fragments arranged by size:
1471 1135 980 720 681 662 452 374
275 233 219 191 131 84 52
SfiI GGCCn_nnn'nGGCC
Cuts at: 2132 4367 2132
Size: 2235 5425
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7660
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7660
SmaI CCC'GGG
Cuts at: 1872 3779 4987 1872
Size: 1907 1208 4545
Fragments arranged by size:
4545 1907 1208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5764 6665 6927 820
Size: 197 926 3821 901 262 1553
Fragments arranged by size:
3821 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4271 5451 493
Size: 3778 1180 2702
Fragments arranged by size:
3778 2702 1180
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7660
SphI G_CATG'C
Cuts at: 1132 5018 1132
Size: 3886 3774
SrfI GCCC'GGGC
Cuts at: 3779 3779
Size: 7660
SspI AAT'ATT
Cuts at: 5 52 2432 5584 5
Size: 47 2380 3152 2081
Fragments arranged by size:
3152 2380 2081 47
StuI AGG'CCT
Cuts at: 1517 2120 3872 4826 1517
Size: 603 1752 954 4351
Fragments arranged by size:
4351 1752 954 603
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2164 2532 3087 3118 3351
Size: 552 939 111 49 368 555 31 233
Cuts at: 3351 3517 4320 4467 4761 4888 5099 513
Size: 166 803 147 294 127 211 3074
Fragments arranged by size:
3074 939 803 555 552 368 294 233
211 166 147 127 111 49 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3923 4022 5687 5704 5857 6042 6487
Size: 1255 2343 99 1665 17 153 185 445
Cuts at: 6487 325
Size: 1498
Fragments arranged by size:
2343 1665 1498 1255 445 185 153 99
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2666 3549 4086
Size: 274 80 33 51 1443 689 883 537
Cuts at: 4086 5906 7618 96
Size: 1820 1712 138
Fragments arranged by size:
1820 1712 1443 883 689 537 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2260 2292 3099 3131 7620 7652 2260
Size: 32 807 32 4489 32 2268
Fragments arranged by size:
4489 2268 807 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4664 1316
Size: 3348 4312
XbaI T'CTAG_A
Cuts at: 4996 4996
Size: 7660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2539
Size: 7660
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7660
XmaI C'CCGG_G
Cuts at: 1870 3777 4985 1870
Size: 1907 1208 4545
Fragments arranged by size:
4545 1907 1208
XmnI GAAnn'nnTTC
Cuts at: 2246 5789 2246
Size: 3543 4117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5468 276
Size: 53 83 186 936 3934 2468
Fragments arranged by size:
3934 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BsiWI BsmBI EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI
PspXI PsrI RsrII SacI SanDI SapI SfoI SwaI