Restriction map for
pFN21AA1255
(Circular) (Six-base) MAPSORT of: pFN21AA1255.seq Check: 3870 from: 1 to: 8338
pFN21AA1255 8338 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4717 4717
Size: 8338
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5524 6148 278
Size: 53 83 186 936 3988 624 2468
Fragments arranged by size:
3988 2468 936 624 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5681 1937
Size: 3744 4594
Acc65I G'GTAC_C
Cuts at: 5659 5659
Size: 8338
AclI AA'CG_TT
Cuts at: 1563 6465 6838 1563
Size: 4902 373 3063
Fragments arranged by size:
4902 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 3859 4289 6401 7785 8053 1632
Size: 728 1499 430 2112 1384 268 1917
Fragments arranged by size:
2112 1917 1499 1384 728 430 268
AfeI AGC'GCT
Cuts at: 2228 2749 2228
Size: 521 7817
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8141
AflIII A'CryG_T
Cuts at: 1203 3244 4170 7237 8214 1203
Size: 2041 926 3067 977 1327
Fragments arranged by size:
3067 2041 1327 977 926
AgeI A'CCGG_T
Cuts at: 7169 7169
Size: 8338
AhdI GACnn_n'nnGTC
Cuts at: 3985 7067 3985
Size: 3082 5256
AleI CACnn'nnGTG
Cuts at: 1166 2367 1166
Size: 1201 7137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2385 3216 5285 5288 1184
Size: 1201 831 2069 3 4234
Fragments arranged by size:
4234 2069 1201 831 3
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2513 2545 2513
Size: 32 8306
AlwNI CAG_nnn'CTG
Cuts at: 2628 3289 3955 4374 4981 5401 6081 7653 2628
Size: 661 666 419 607 420 680 1572 3313
Fragments arranged by size:
3313 1572 680 666 661 607 420 419
ApaI G_GGCC'C
Cuts at: 2086 2086
Size: 8338
ApaLI G'TGCA_C
Cuts at: 5113 5467 6395 7551 5113
Size: 354 928 1156 5900
Fragments arranged by size:
5900 1156 928 354
ApoI r'AATT_y
Cuts at: 1493 4747 4822 5647 5873 6025 8134 1493
Size: 3254 75 825 226 152 2109 1697
Fragments arranged by size:
3254 2109 1697 825 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 6892 160
Size: 872 5860 1606
Fragments arranged by size:
5860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 3891 4158 5339 5663 1870
Size: 73 143 1805 267 1181 324 4545
Fragments arranged by size:
4545 1805 1181 324 267 143 73
BamHI G'GATC_C
Cuts at: 5668 6035 5668
Size: 367 7971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 3092 3200 3572 3671
Size: 325 217 627 334 971 108 372 99
Cuts at: 3671 4299 4403 4508 5659 7114 618
Size: 628 104 105 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 971 628 627 372 334
325 217 108 105 104 99
BanII G_rGCy'C
Cuts at: 1391 2086 3660 3962 4347 5335 5657 1391
Size: 695 1574 302 385 988 322 4072
Fragments arranged by size:
4072 1574 988 695 385 322 302
BbeI G_GCGC'C
Cuts at: 3675 4407 3675
Size: 732 7606
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2418 2824 2829 3366 4487 5516 928
Size: 971 519 406 5 537 1121 1029 3750
Fragments arranged by size:
3750 1121 1029 971 537 519 406 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2461 2495 6529 6563 1903
Size: 34 50 34 440 34 4034 34 3678
Fragments arranged by size:
4034 3678 440 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5244 6230 7440 5244
Size: 986 1210 6142
Fragments arranged by size:
6142 1210 986
BclI T'GATC_A
Cuts at: 1548 2769 4800 1548
Size: 1221 2031 5086
Fragments arranged by size:
5086 2031 1221
BfrBI ATG'CAT
Cuts at: 5223 5223
Size: 8338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2046 2090 2303
Size: 107 122 71 631 605 374 44 213
Cuts at: 2303 6949 136
Size: 4646 1525
Fragments arranged by size:
4646 1525 631 605 374 213 122 107
71 44
BglII A'GATC_T
Cuts at: 2899 2911 3031 8333 2899
Size: 12 120 5302 2904
Fragments arranged by size:
5302 2904 120 12
BlpI GC'TnA_GC
Cuts at: 4722 5755 4722
Size: 1033 7305
Bme1580I G_kGCm'C
Cuts at: 1790 2086 3097 3577 5117 5471 6399 7555 1790
Size: 296 1011 480 1540 354 928 1156 2573
Fragments arranged by size:
2573 1540 1156 1011 928 480 354 296
BmgBI CAC'GTC
Cuts at: 1318 2827 4381 1318
Size: 1509 1554 5275
Fragments arranged by size:
5275 1554 1509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3098 6069 7027
Size: 416 53 83 399 24 1681 2971 958
Cuts at: 7027 442
Size: 1753
Fragments arranged by size:
2971 1753 1681 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3050 3050
Size: 8338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2428 2528 2954 3019 3549 3839 3984 4076
Size: 1189 100 426 65 530 290 145 92
Cuts at: 4076 4091 4250 4520 6998 1239
Size: 15 159 270 2478 2579
Fragments arranged by size:
2579 2478 1189 530 426 290 270 159
145 100 92 65 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6463 7328 7626 6463
Size: 865 298 7175
Fragments arranged by size:
7175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3888 7001 882
Size: 617 2389 3113 2219
Fragments arranged by size:
3113 2389 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4555 6129 493
Size: 711 3351 1574 2702
Fragments arranged by size:
3351 2702 1574 711
BsaBI GATnn'nnATC
Cuts at: 2457 8332 2457
Size: 5875 2463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 3672 3764 4404
Size: 53 83 186 936 597 1542 92 640
Cuts at: 4404 5124 5521 6145 6527 7162 275
Size: 720 397 624 382 635 1451
Fragments arranged by size:
1542 1451 936 720 640 635 624 597
397 382 186 92 83 53
BsaWI w'CCGG_w
Cuts at: 3796 3868 5322 6771 7169 7443 7590 8001 3796
Size: 72 1454 1449 398 274 147 411 4133
Fragments arranged by size:
4133 1454 1449 411 398 274 147 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4908 4938 5423 5453 1178
Size: 30 165 30 3505 30 485 30 4063
Fragments arranged by size:
4063 3505 485 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2567 2669 3042 4176 1186
Size: 1381 102 373 1134 5348
Fragments arranged by size:
5348 1381 1134 373 102
BseYI C'CCAG_C
Cuts at: 2859 3907 3948 3955 4278 4971 7541 8037 2859
Size: 1048 41 7 323 693 2570 496 3160
Fragments arranged by size:
3160 2570 1048 693 496 323 41 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2092 2709 3095 3377 3755 3992 4301
Size: 573 356 617 386 282 378 237 309
Cuts at: 4301 4844 5144 1163
Size: 543 300 4357
Fragments arranged by size:
4357 617 573 543 386 378 356 309
300 282 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2029 3942 5080 5327 5788 6549
Size: 275 186 28 1913 1138 247 461 761
Cuts at: 6549 7577 1540
Size: 1028 2301
Fragments arranged by size:
2301 1913 1138 1028 761 461 275 247
186 28
BsiHKAI G_wGCw'C
Cuts at: 2374 3660 4062 5117 5471 6399 6484 7555 2374
Size: 1286 402 1055 354 928 85 1071 3157
Fragments arranged by size:
3157 1286 1071 1055 928 402 354 85
BsmI GAATG_Cn'
Cuts at: 2668 5194 5225 5849 5942 2668
Size: 2526 31 624 93 5064
Fragments arranged by size:
5064 2526 624 93 31
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2374 3097 3577 3660 3962 4062
Size: 399 296 288 723 480 83 302 100
Cuts at: 4062 4347 5117 5335 5471 5657 6399 6484 7555
Size: 285 770 218 136 186 742 85 1071
Cuts at: 7555 1391
Size: 2174
Fragments arranged by size:
2174 1071 770 742 723 480 399 302
296 288 285 218 186 136 100 85
83
BspEI T'CCGG_A
Cuts at: 3796 3868 8001 3796
Size: 72 4133 4133
Fragments arranged by size:
4133 4133 72
BspHI T'CATG_A
Cuts at: 6227 7957 6227
Size: 1730 6608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2040 3693 4717 4989 5693 844
Size: 1196 1653 1024 272 704 3489
Fragments arranged by size:
3489 1653 1196 1024 704 272
BsrBI CCG'CTC
Cuts at: 1394 1823 6225 1394
Size: 429 4402 3507
Fragments arranged by size:
4402 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6833 7007 65
Size: 1183 5585 174 1396
Fragments arranged by size:
5585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2060 2115 5079 6982 7169 1700
Size: 110 218 32 55 2964 1903 187 2869
Fragments arranged by size:
2964 2869 1903 218 187 110 55 32
BsrGI T'GTAC_A
Cuts at: 96 3126 96
Size: 3030 5308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8338
BssSI C'ACGA_G
Cuts at: 3326 6398 7410 3326
Size: 3072 1012 4254
Fragments arranged by size:
4254 3072 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 4529 4981 794
Size: 1376 2359 452 4151
Fragments arranged by size:
4151 2359 1376 452
BstBI TT'CG_AA
Cuts at: 3399 7142 3399
Size: 3743 4595
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8338
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 2867 2953 4555 2812
Size: 55 86 1602 6595
Fragments arranged by size:
6595 1602 86 55
BstYI r'GATC_y
Cuts at: 1972 2490 2899 2911 3031 5668 6035 6420 6437
Size: 518 409 12 120 2637 367 385 17
Cuts at: 6437 7889 7975 7987 8333 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2637 1977 1452 518 409 385 367 346
120 86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2463 2579 2720 2463
Size: 116 141 8081
Fragments arranged by size:
8081 141 116
BtgI C'CryG_G
Cuts at: 513 1065 2015 2077 3411 5031 5438 513
Size: 552 950 62 1334 1620 407 3413
Fragments arranged by size:
3413 1620 1334 950 552 407 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4222 4928 487
Size: 683 56 2996 706 3897
Fragments arranged by size:
3897 2996 706 683 56
BtsI GCAGTG_nn'
Cuts at: 2404 3107 3299 3360 3767 4375 5156 5469 5512
Size: 703 192 61 407 608 781 313 43
Cuts at: 5512 5854 6648 6668 2404
Size: 342 794 20 4074
Fragments arranged by size:
4074 794 781 703 608 407 342 313
192 61 43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8303
DraI TTT'AAA
Cuts at: 5642 5642
Size: 8338
DraIII CAC_nnn'GTG
Cuts at: 3092 3118 3624 3092
Size: 26 506 7806
Fragments arranged by size:
7806 506 26
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7345 809
Size: 507 6029 1802
Fragments arranged by size:
6029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2148 2295
Size: 54 1425 50 275 18 196 122 147
Cuts at: 2295 3258 3639 4014 4113 4557 5077 5196 5785
Size: 963 381 375 99 444 520 119 589
Cuts at: 5785 6674 8303 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 963 889 589 520 444 381
375 275 196 147 122 119 99 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2026 5077 5785 1537
Size: 275 214 3051 708 4090
Fragments arranged by size:
4090 3051 708 275 214
EarI CTCTTCn'nnn_
Cuts at: 1353 3843 4052 4419 4473 5061 5216 6268 1353
Size: 2490 209 367 54 588 155 1052 3423
Fragments arranged by size:
3423 2490 1052 588 367 209 155 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2228 3758 6925 7299 7445 387
Size: 1064 138 639 1530 3167 374 146 1280
Fragments arranged by size:
3167 1530 1280 1064 639 374 146 138
EcoICRI GAG'CTC
Cuts at: 3658 3658
Size: 8338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2428 2528 2954 3019 3549 3839
Size: 393 728 68 100 426 65 530 290
Cuts at: 3839 3859 3984 4076 4091 4250 4289 4520 6401
Size: 20 125 92 15 159 39 231 1881
Cuts at: 6401 6998 7785 8053 1239
Size: 597 787 268 1524
Fragments arranged by size:
1881 1524 787 728 597 530 426 393
290 268 231 159 125 100 92 68
65 39 20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2723 3191 2723
Size: 468 7870
EcoO109I rG'GnC_Cy
Cuts at: 2052 2082 2083 3066 3184 4289 4978 2052
Size: 30 1 983 118 1105 689 5412
Fragments arranged by size:
5412 1105 983 689 118 30 1
EcoRI G'AATT_C
Cuts at: 4822 5647 6025 4822
Size: 825 378 7135
Fragments arranged by size:
7135 825 378
EcoRV GAT'ATC
Cuts at: 4612 4612
Size: 8338
FspI TGC'GCA
Cuts at: 2247 6844 2247
Size: 4597 3741
HaeII r_GCGC'y
Cuts at: 1678 2230 2751 3675 4407 7485 1678
Size: 552 521 924 732 3078 2531
Fragments arranged by size:
3078 2531 924 732 552 521
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3143 3175 3710 3742 3791
Size: 32 135 32 1571 32 535 32 49
Cuts at: 3791 3823 3957 3971 3989 4003 4226 4258 5352
Size: 32 134 14 18 14 223 32 1094
Cuts at: 5352 5384 5408 5440 6979 7011 7053 7085 1373
Size: 32 24 32 1539 32 42 32 2626
Fragments arranged by size:
2626 1571 1539 1094 535 223 135 134
49 42 32 32 32 32 32 32
32 32 32 32 24 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3076 3583 4851 5365 5682 5928
Size: 988 281 1138 507 1268 514 317 246
Cuts at: 5928 669
Size: 3079
Fragments arranged by size:
3079 1268 1138 988 514 507 317 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8338
HpaI GTT'AAC
Cuts at: 5928 5928
Size: 8338
KasI G'GCGC_C
Cuts at: 3671 4403 3671
Size: 732 7606
KpnI G_GTAC'C
Cuts at: 5663 5663
Size: 8338
MfeI C'AATT_G
Cuts at: 5937 5937
Size: 8338
MluI A'CGCG_T
Cuts at: 8214 8214
Size: 8338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 2421 3969 5431 7452 7636 1305
Size: 831 285 1548 1462 2021 184 2007
Fragments arranged by size:
2021 2007 1548 1462 831 285 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 2297 3641 4016 4115
Size: 54 1425 343 318 147 1344 375 99
Cuts at: 4115 4559 10
Size: 444 3789
Fragments arranged by size:
3789 1425 1344 444 375 343 318 147
99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2675 2810 3798 4175 4263 4553
Size: 648 1201 308 135 988 377 88 290
Cuts at: 4553 4620 4815 6296 6655 6814 518
Size: 67 195 1481 359 159 2042
Fragments arranged by size:
2042 1481 1201 988 648 377 359 308
290 195 159 135 88 67
MspA1I CmG'CkG
Cuts at: 1606 2017 2079 2406 3286 3520 3527 3952 4024
Size: 411 62 327 880 234 7 425 72
Cuts at: 4024 4111 4204 4591 5195 5754 6431 7579 7824
Size: 87 93 387 604 559 677 1148 245
Cuts at: 7824 1606
Size: 2120
Fragments arranged by size:
2120 1148 880 677 604 559 425 411
387 327 245 234 93 87 72 62
7
NaeI GCC'GGC
Cuts at: 2030 2117 5081 2030
Size: 87 2964 5287
Fragments arranged by size:
5287 2964 87
NarI GG'CG_CC
Cuts at: 3672 4404 3672
Size: 732 7606
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7786
NdeI CA'TA_TG
Cuts at: 387 4151 387
Size: 3764 4574
NgoMIV G'CCGG_C
Cuts at: 2028 2115 5079 2028
Size: 87 2964 5287
Fragments arranged by size:
5287 2964 87
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8338
NotI GC'GGCC_GC
Cuts at: 5785 5785
Size: 8338
NsiI A_TGCA'T
Cuts at: 5225 5225
Size: 8338
NspI r_CATG'y
Cuts at: 1132 1229 3217 3248 3934 4174 4278 5227 5696
Size: 97 1988 31 686 240 104 949 469
Cuts at: 5696 7241 1132
Size: 1545 2229
Fragments arranged by size:
2229 1988 1545 949 686 469 240 104
97 31
PasI CC'CwG_GG
Cuts at: 1348 2867 1348
Size: 1519 6819
PciI A'CATG_T
Cuts at: 3244 4170 7237 3244
Size: 926 3067 4345
Fragments arranged by size:
4345 3067 926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3955 4278 1108
Size: 2847 323 5168
Fragments arranged by size:
5168 2847 323
PfoI T'CCnGG_A
Cuts at: 3816 4285 4833 3816
Size: 469 548 7321
Fragments arranged by size:
7321 548 469
PmeI GTTT'AAAC
Cuts at: 5642 5642
Size: 8338
PmlI CAC'GTG
Cuts at: 4555 4555
Size: 8338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2495 2527 6407 6439 7920 7952 2495
Size: 32 3880 32 1481 32 2881
Fragments arranged by size:
3880 2881 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2052 4289 4978 2052
Size: 2237 689 5412
Fragments arranged by size:
5412 2237 689
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8338
PsiI TTA'TAA
Cuts at: 5564 5908 5564
Size: 344 7994
PspOMI G'GGCC_C
Cuts at: 2082 2082
Size: 8338
PstI C_TGCA'G
Cuts at: 830 2202 3195 3294 3666 3690 3762 5690 830
Size: 1372 993 99 372 24 72 1928 3478
Fragments arranged by size:
3478 1928 1372 993 372 99 72 24
PvuI CG_AT'CG
Cuts at: 2001 3942 2001
Size: 1941 6397
PvuII CAG'CTG
Cuts at: 2406 3286 3527 3952 4111 4591 2406
Size: 880 241 425 159 480 6153
Fragments arranged by size:
6153 880 480 425 241 159
SacI G_AGCT'C
Cuts at: 3660 3660
Size: 8338
SacII CC_GC'GG
Cuts at: 2018 2080 2018
Size: 62 8276
SalI G'TCGA_C
Cuts at: 1936 5680 1936
Size: 3744 4594
SapI GCTCTTCn'nnn_
Cuts at: 3843 5061 3843
Size: 1218 7120
SbfI CC_TGCA'GG
Cuts at: 5690 5690
Size: 8338
ScaI AGT'ACT
Cuts at: 6586 8298 6586
Size: 1712 6626
SexAI A'CCwGG_T
Cuts at: 4291 5379 4291
Size: 1088 7250
SfcI C'TryA_G
Cuts at: 826 1045 2198 2570 3191 3290 3515 3662 3686
Size: 219 1153 372 621 99 225 147 24
Cuts at: 3686 3758 5686 6821 7502 7693 826
Size: 72 1928 1135 681 191 1471
Fragments arranged by size:
1928 1471 1153 1135 681 621 372 225
219 191 147 99 72 24
SfoI GGC'GCC
Cuts at: 3673 4405 3673
Size: 732 7606
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
SgrAI Cr'CCGG_yG
Cuts at: 1700 2060 1700
Size: 360 7978
SmaI CCC'GGG
Cuts at: 1872 5665 1872
Size: 3793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6442 7343 7605 820
Size: 197 926 4499 901 262 1553
Fragments arranged by size:
4499 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6129 493
Size: 5636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8338
SphI G_CATG'C
Cuts at: 1132 3934 5227 5696 1132
Size: 2802 1293 469 3774
Fragments arranged by size:
3774 2802 1293 469
SspI AAT'ATT
Cuts at: 5 52 6262 5
Size: 47 6210 2081
Fragments arranged by size:
6210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8338
StyI C'CwwG_G
Cuts at: 513 1065 5427 5547 5777 513
Size: 552 4362 120 230 3074
Fragments arranged by size:
4362 3074 552 230 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3080 4287 5615 6365 6382 6535 6720
Size: 1255 1500 1207 1328 750 17 153 185
Cuts at: 6720 7165 325
Size: 445 1498
Fragments arranged by size:
1500 1498 1328 1255 1207 750 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2328 3126 4486
Size: 274 80 33 51 1443 351 798 1360
Cuts at: 4486 5614 6584 8296 96
Size: 1128 970 1712 138
Fragments arranged by size:
1712 1443 1360 1128 970 798 351 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2130 2162 4908 4940 5423 5455 8298 8330 2130
Size: 32 2746 32 483 32 2843 32 2138
Fragments arranged by size:
2843 2746 2138 483 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2828 3749 1316
Size: 1512 921 5905
Fragments arranged by size:
5905 1512 921
XbaI T'CTAG_A
Cuts at: 5674 5674
Size: 8338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2437 2437
Size: 8338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8338
XmaI C'CCGG_G
Cuts at: 1870 5663 1870
Size: 3793 4545
XmnI GAAnn'nnTTC
Cuts at: 3395 6467 3395
Size: 3072 5266
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5522 6146 276
Size: 53 83 186 936 3988 624 2468
Fragments arranged by size:
3988 2468 936 624 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I
FalI FseI FspAI NruI PacI PspXI PsrI RsrII
SanDI SfiI SrfI SwaI