Restriction map for
pFN21AA1238
(Circular) (Six-base) MAPSORT of: pFN21AA1238.seq Check: 1106 from: 1 to: 5863
pFN21AA1238 5863 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 1937
Size: 1269 4594
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2469 3926 5310 5578 1632
Size: 837 1457 1384 268 1917
Fragments arranged by size:
1917 1457 1384 837 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 2617 4762 5739 1203
Size: 1414 2145 977 1327
Fragments arranged by size:
2145 1414 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 2046 4592 2046
Size: 2546 3317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2813 1184
Size: 1629 4234
AlwNI CAG_nnn'CTG
Cuts at: 3606 5178 3606
Size: 1572 4291
ApaLI G'TGCA_C
Cuts at: 3920 5076 3920
Size: 1156 4707
ApoI r'AATT_y
Cuts at: 1493 2422 3172 3398 3550 5659 1493
Size: 929 750 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 750 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 3188 1870
Size: 73 574 671 4545
Fragments arranged by size:
4545 671 574 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2648 3184 4639 618
Size: 325 217 627 861 536 1455 1842
Fragments arranged by size:
1842 1455 861 627 536 325 217
BanII G_rGCy'C
Cuts at: 1391 3182 1391
Size: 1791 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2067 928
Size: 971 168 4724
Fragments arranged by size:
4724 971 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2408 2442 4054 4088 1903
Size: 34 471 34 1612 34 3678
Fragments arranged by size:
3678 1612 471 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2389 3755 4965 2389
Size: 1366 1210 3287
Fragments arranged by size:
3287 1366 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4474 136
Size: 107 122 71 631 605 2802 1525
Fragments arranged by size:
2802 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 3924 5080 1790
Size: 2134 1156 2573
Fragments arranged by size:
2573 2134 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3594 4552 442
Size: 416 53 83 399 24 2177 958 1753
Fragments arranged by size:
2177 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2750 3137 1056
Size: 1694 387 3782
Fragments arranged by size:
3782 1694 387
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3107
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 4523 1239
Size: 1238 2046 2579
Fragments arranged by size:
2579 2046 1238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2307 3988 4853 5151 2307
Size: 1681 865 298 3019
Fragments arranged by size:
3019 1681 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4526 882
Size: 617 3027 2219
Fragments arranged by size:
3027 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2618 3654 493
Size: 711 1414 1036 2702
Fragments arranged by size:
2702 1414 1036 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4296 4694 4968 5115 5526 4296
Size: 398 274 147 411 4633
Fragments arranged by size:
4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2625 2655 1178
Size: 30 165 30 1222 30 4386
Fragments arranged by size:
4386 1222 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 1186
Size: 940 4923
BseYI C'CCAG_C
Cuts at: 3001 3010 5066 5562 3001
Size: 9 2056 496 3302
Fragments arranged by size:
3302 2056 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3313 4074 5102 1540
Size: 275 186 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2723 3924 4009 5080 2723
Size: 1201 85 1071 3506
Fragments arranged by size:
3506 1201 1071 85
BsmI GAATG_Cn'
Cuts at: 2685 3374 3467 2685
Size: 689 93 5081
Fragments arranged by size:
5081 689 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2723 3182 3924 4009 5080 1391
Size: 399 933 459 742 85 1071 2174
Fragments arranged by size:
2174 1071 933 742 459 399 85
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2805 3218 844
Size: 1961 413 3489
Fragments arranged by size:
3489 1961 413
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4358 4532 65
Size: 1183 3110 174 1396
Fragments arranged by size:
3110 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2934 4507 4694 1700
Size: 110 1124 1573 187 2869
Fragments arranged by size:
2869 1573 1124 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2909 794
Size: 2115 3748
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 2247 2247
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858
Size: 1221 367 385 17 1452 86 12 346
Cuts at: 5858 1972
Size: 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2377 2964 513
Size: 552 1312 587 3412
Fragments arranged by size:
3412 1312 587 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2842 487
Size: 683 56 1616 3508
Fragments arranged by size:
3508 1616 683 56
BtsI GCAGTG_nn'
Cuts at: 2205 2973 3018 3379 4173 4193 2205
Size: 768 45 361 794 20 3875
Fragments arranged by size:
3875 794 768 361 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2374 2413 2932
Size: 54 1425 50 275 18 544 39 519
Cuts at: 2932 3310 4199 5828 8
Size: 378 889 1629 43
Fragments arranged by size:
1629 1425 889 544 519 378 275 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3793 1353
Size: 2440 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4450 4824 4970 387
Size: 1064 138 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 2477 3926 4523 5310 5578 1239
Size: 393 837 8 1449 597 787 268 1524
Fragments arranged by size:
1524 1449 837 787 597 393 268 8
EcoO109I rG'GnC_Cy
Cuts at: 3005 3109 3005
Size: 104 5759
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 2570 5010 1678
Size: 892 2440 2531
Fragments arranged by size:
2531 2440 892
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2064 2187 2219 2287
Size: 32 135 32 460 32 123 32 68
Cuts at: 2287 2319 2387 2419 2801 2833 4504 4536 4578
Size: 32 68 32 382 32 1671 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 1671 460 382 135 123 68 68
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3028 3058 3207 3453 669
Size: 988 281 1090 30 149 246 3079
Fragments arranged by size:
3079 1090 988 281 246 149 30
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4977 5161 1305
Size: 3672 184 2007
Fragments arranged by size:
3672 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2376 2415 10
Size: 54 1425 343 544 39 3458
Fragments arranged by size:
3458 1425 544 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 3821 4180 4339 518
Size: 648 1461 1194 359 159 2042
Fragments arranged by size:
2042 1461 1194 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3130 3279 3956 5104 5349 1606
Size: 1524 149 677 1148 245 2120
Fragments arranged by size:
2120 1524 1148 677 245 149
NaeI GCC'GGC
Cuts at: 2936 2936
Size: 5863
NcoI C'CATG_G
Cuts at: 513 1065 2377 513
Size: 552 1312 3999
Fragments arranged by size:
3999 1312 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NgoMIV G'CCGG_C
Cuts at: 2934 2934
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 2746 3133 1052
Size: 1694 387 3782
Fragments arranged by size:
3782 1694 387
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 3221 4766 1132
Size: 97 1992 1545 2229
Fragments arranged by size:
2229 1992 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3104 1348
Size: 1756 4107
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2031 1108
Size: 923 4940
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3060 3932 3964 5445 5477 3028
Size: 32 872 32 1481 32 3414
Fragments arranged by size:
3414 1481 872 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3005 3005
Size: 5863
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PstI C_TGCA'G
Cuts at: 830 3215 830
Size: 2385 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 3130 3130
Size: 5863
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SanDI GG'GwC_CC
Cuts at: 3005 3005
Size: 5863
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SfcI C'TryA_G
Cuts at: 826 1045 3211 4346 5027 5218 826
Size: 219 2166 1135 681 191 1471
Fragments arranged by size:
2166 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2322 2517 3967 4868 5130 820
Size: 197 926 379 195 1450 901 262 1553
Fragments arranged by size:
1553 1450 926 901 379 262 197 195
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 2987 152
Size: 2835 3028
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2246 2377 3302 513
Size: 552 1181 131 925 3074
Fragments arranged by size:
3074 1181 925 552 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 3890 3907 4060 4245 4690 325
Size: 1255 659 1651 17 153 185 445 1498
Fragments arranged by size:
1651 1498 1255 659 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4109 5821 96
Size: 274 80 33 51 1443 2132 1712 138
Fragments arranged by size:
2132 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2625 2657 5823 5855 2625
Size: 32 3166 32 2633
Fragments arranged by size:
3166 2633 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 2287 2881 3199 2287
Size: 594 318 4951
Fragments arranged by size:
4951 594 318
XhoI C'TCGA_G
Cuts at: 1943 2517 1943
Size: 574 5289
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI