Restriction map for
pFN21AA1237
(Circular) (Six-base) MAPSORT of: pFN21AA1237.seq Check: 8078 from: 1 to: 9151
pFN21AA1237 9151 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2643 5331 2643
Size: 2688 6463
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6961 278
Size: 53 83 186 936 5425 2468
Fragments arranged by size:
5425 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4418 6494 1937
Size: 2481 2076 4594
Fragments arranged by size:
4594 2481 2076
Acc65I G'GTAC_C
Cuts at: 5469 6472 5469
Size: 1003 8148
AclI AA'CG_TT
Cuts at: 1563 3589 5603 7278 7651 1563
Size: 2026 2014 1675 373 3063
Fragments arranged by size:
3063 2026 2014 1675 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3438 3459 4305 4868 5202 5238 7214 8598
Size: 1806 21 846 563 334 36 1976 1384
Cuts at: 8598 8866 1632
Size: 268 1917
Fragments arranged by size:
1976 1917 1806 1384 846 563 334 268
36 21
AflII C'TTAA_G
Cuts at: 820 1017 4640 820
Size: 197 3623 5331
Fragments arranged by size:
5331 3623 197
AflIII A'CryG_T
Cuts at: 1203 8050 9027 1203
Size: 6847 977 1327
Fragments arranged by size:
6847 1327 977
AgeI A'CCGG_T
Cuts at: 7982 7982
Size: 9151
AhdI GACnn_n'nnGTC
Cuts at: 3051 7880 3051
Size: 4829 4322
AleI CACnn'nnGTG
Cuts at: 1166 6059 1166
Size: 4893 4258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9151
AlwNI CAG_nnn'CTG
Cuts at: 6894 8466 6894
Size: 1572 7579
ApaI G_GGCC'C
Cuts at: 2232 2260 2305 2232
Size: 28 45 9078
Fragments arranged by size:
9078 45 28
ApaLI G'TGCA_C
Cuts at: 2750 3885 7208 8364 2750
Size: 1135 3323 1156 3537
Fragments arranged by size:
3537 3323 1156 1135
ApoI r'AATT_y
Cuts at: 1493 2842 3191 3218 3565 6460 6686 6838 8947
Size: 1349 349 27 347 2895 226 152 2109
Cuts at: 8947 1493
Size: 1697
Fragments arranged by size:
2895 2109 1697 1349 349 347 226 152
27
AseI AT'TA_AT
Cuts at: 160 1032 4227 7705 160
Size: 872 3195 3478 1606
Fragments arranged by size:
3478 3195 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9151
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2214 3025 6476 1870
Size: 73 147 124 811 3451 4545
Fragments arranged by size:
4545 3451 811 147 124 73
AvrII C'CTAG_G
Cuts at: 6097 6097
Size: 9151
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5049 5082 5049
Size: 33 9118
BamHI G'GATC_C
Cuts at: 5441 6481 6848 5441
Size: 1040 367 7744
Fragments arranged by size:
7744 1040 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 2270 3670 3683 3818
Size: 325 217 627 353 130 1400 13 135
Cuts at: 3818 4880 5343 5469 6472 7927 618
Size: 1062 463 126 1003 1455 1842
Fragments arranged by size:
1842 1455 1400 1062 1003 627 463 353
325 217 135 130 126 13
BanII G_rGCy'C
Cuts at: 1391 2232 2260 2305 2786 2969 4058 6087 6470
Size: 841 28 45 481 183 1089 2029 383
Cuts at: 6470 1391
Size: 4072
Fragments arranged by size:
4072 2029 1089 841 481 383 183 45
28
BbeI G_GCGC'C
Cuts at: 2144 2274 2144
Size: 130 9021
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2621 3528 3609 4437 4856 5190 5282
Size: 971 722 907 81 828 419 334 92
Cuts at: 5282 928
Size: 4797
Fragments arranged by size:
4797 971 907 828 722 419 334 92
81
BbvCI CC'TCA_GC
Cuts at: 5781 5781
Size: 9151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3016 3050 3129 3163 7342 7376 1903
Size: 34 1079 34 79 34 4179 34 3678
Fragments arranged by size:
4179 3678 1079 79 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4480 5915 7043 8253 4480
Size: 1435 1128 1210 5378
Fragments arranged by size:
5378 1435 1210 1128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9151
BfrBI ATG'CAT
Cuts at: 2995 4430 6250 2995
Size: 1435 1820 5896
Fragments arranged by size:
5896 1820 1435
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5356 6012 7762
Size: 107 122 71 631 605 3684 656 1750
Cuts at: 7762 136
Size: 1525
Fragments arranged by size:
3684 1750 1525 656 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2857 9146 2857
Size: 6289 2862
BlpI GC'TnA_GC
Cuts at: 2132 4164 6568 2132
Size: 2032 2404 4715
Fragments arranged by size:
4715 2404 2032
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2260 2305 2754 3675 3889 5348 7212
Size: 442 28 45 449 921 214 1459 1864
Cuts at: 7212 8368 1790
Size: 1156 2573
Fragments arranged by size:
2573 1864 1459 1156 921 449 442 214
45 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 3460 6882
Size: 416 53 83 399 24 1763 280 3422
Cuts at: 6882 7840 442
Size: 958 1753
Fragments arranged by size:
3422 1763 1753 958 416 399 280 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3838 4120 6101 3838
Size: 282 1981 6888
Fragments arranged by size:
6888 1981 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2195 2460 2597 2945 3079 3513 3865
Size: 944 12 265 137 348 134 434 352
Cuts at: 3865 6263 7811 1239
Size: 2398 1548 2579
Fragments arranged by size:
2579 2398 1548 944 434 352 348 265
137 134 12
Bpu10I CC'TnA_GC
Cuts at: 2262 5781 2262
Size: 3519 5632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7276 8141 8439 7276
Size: 865 298 7988
Fragments arranged by size:
7988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3865 6029 7814 882
Size: 617 2366 2164 1785 2219
Fragments arranged by size:
2366 2219 2164 1785 617
BsaAI yAC'GTr
Cuts at: 493 1204 3581 3832 6942 493
Size: 711 2377 251 3110 2702
Fragments arranged by size:
3110 2702 2377 711 251
BsaBI GATnn'nnATC
Cuts at: 9145 9145
Size: 9151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 2271 6958 7340
Size: 53 83 186 936 608 130 4687 382
Cuts at: 7340 7975 275
Size: 635 1451
Fragments arranged by size:
4687 1451 936 635 608 382 186 130
83 53
BsaWI w'CCGG_w
Cuts at: 7584 7982 8256 8403 8814 7584
Size: 398 274 147 411 7921
Fragments arranged by size:
7921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4271 4301 4729 4759 1178
Size: 30 165 30 2868 30 428 30 5570
Fragments arranged by size:
5570 2868 428 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2889 2959 4273 4276 4279 4282 4291 4294
Size: 1703 70 1314 3 3 3 9 3
Cuts at: 4294 4297 6266 1186
Size: 3 1969 4071
Fragments arranged by size:
4071 1969 1703 1314 70 9 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2246 3899 5122 8354 8850 2246
Size: 1653 1223 3232 496 2547
Fragments arranged by size:
3232 2547 1653 1223 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3357 3812 5863 1163
Size: 573 1621 455 2051 4451
Fragments arranged by size:
4451 2051 1621 573 455
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2299 6601 7362 8390 1540
Size: 275 186 298 4302 761 1028 2301
Fragments arranged by size:
4302 2301 1028 761 298 275 186
BsiHKAI G_wGCw'C
Cuts at: 2754 2969 3764 3889 4058 4655 7212 7297 8368
Size: 215 795 125 169 597 2557 85 1071
Cuts at: 8368 2754
Size: 3537
Fragments arranged by size:
3537 2557 1071 795 597 215 169 125
85
BsmI GAATG_Cn'
Cuts at: 3454 5309 6379 6662 6755 3454
Size: 1855 1070 283 93 5850
Fragments arranged by size:
5850 1855 1070 283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2260 2305 2754 2786 2969 3675
Size: 399 442 28 45 449 32 183 706
Cuts at: 3675 3764 3889 4058 4655 5348 6087 6470 7212
Size: 89 125 169 597 693 739 383 742
Cuts at: 7212 7297 8368 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 706 693 597 449
442 399 383 183 169 125 89 85
45 32 28
BspEI T'CCGG_A
Cuts at: 8814 8814
Size: 9151
BspHI T'CATG_A
Cuts at: 4605 7040 8770 4605
Size: 2435 1730 4986
Fragments arranged by size:
4986 2435 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2643 5331 6002 6506 844
Size: 1799 2688 671 504 3489
Fragments arranged by size:
3489 2688 1799 671 504
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 2156 2526 5825 7038 1394
Size: 429 218 115 370 3299 1213 3507
Fragments arranged by size:
3507 3299 1213 429 370 218 115
BsrDI GCAATG_nn'
Cuts at: 65 1248 7646 7820 65
Size: 1183 6398 174 1396
Fragments arranged by size:
6398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 7795 7982 1700
Size: 110 270 5715 187 2869
Fragments arranged by size:
5715 2869 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 3484 96
Size: 3388 5763
BssHII G'CGCG_C
Cuts at: 1925 2017 2127 1925
Size: 92 110 8949
Fragments arranged by size:
8949 110 92
BssSI C'ACGA_G
Cuts at: 7211 8223 7211
Size: 1012 8139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5835 6003 794
Size: 5041 168 3942
Fragments arranged by size:
5041 3942 168
BstBI TT'CG_AA
Cuts at: 7955 7955
Size: 9151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9151
BstXI CCAn_nnnn'nTGG
Cuts at: 5677 5677
Size: 9151
BstYI r'GATC_y
Cuts at: 1972 2857 5441 5801 5814 6481 6848 7233 7250
Size: 885 2584 360 13 667 367 385 17
Cuts at: 7250 8702 8788 8800 9146 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2584 1977 1452 885 667 385 367 360
346 86 17 13 12
Bsu36I CC'TnA_GG
Cuts at: 2848 2848
Size: 9151
BtgI C'CryG_G
Cuts at: 513 1065 2004 2224 4439 5175 5326 6072 513
Size: 552 939 220 2215 736 151 746 3592
Fragments arranged by size:
3592 2215 939 746 736 552 220 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2738 6088 487
Size: 683 56 1512 3350 3550
Fragments arranged by size:
3550 3350 1512 683 56
BtsI GCAGTG_nn'
Cuts at: 3147 3369 3725 5335 5380 5687 6667 7461 7481
Size: 222 356 1610 45 307 980 794 20
Cuts at: 7481 3147
Size: 4817
Fragments arranged by size:
4817 1610 980 794 356 307 222 45
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5080 5115 529
Size: 35 4516 35 4565
Fragments arranged by size:
4565 4516 35 35
DraI TTT'AAA
Cuts at: 5524 6455 5524
Size: 931 8220
DraIII CAC_nnn'GTG
Cuts at: 3581 3632 3581
Size: 51 9100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8158 809
Size: 507 6842 1802
Fragments arranged by size:
6842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2030 2296 2342
Size: 54 1425 50 275 18 200 266 46
Cuts at: 2342 3795 3873 6598 7487 9116 8
Size: 1453 78 2725 889 1629 43
Fragments arranged by size:
2725 1629 1453 1425 889 275 266 200
78 54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2296 6598 1537
Size: 275 484 4302 4090
Fragments arranged by size:
4302 4090 484 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 2536 2831 4090 4300 4315 5257 5840
Size: 1094 89 295 1259 210 15 942 583
Cuts at: 5840 6433 7081 1353
Size: 593 648 3423
Fragments arranged by size:
3423 1259 1094 942 648 593 583 295
210 89 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3631 5827 7738 8112 8258 387
Size: 1064 138 2042 2196 1911 374 146 1280
Fragments arranged by size:
2196 2042 1911 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2967 4056 2967
Size: 1089 8062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2195 2460 2597 2945 3079 3438
Size: 393 551 12 265 137 348 134 359
Cuts at: 3438 3459 3513 3865 4305 4868 5202 5238 6263
Size: 21 54 352 440 563 334 36 1025
Cuts at: 6263 7214 7811 8598 8866 1239
Size: 951 597 787 268 1524
Fragments arranged by size:
1524 1025 951 787 597 563 551 440
393 359 352 348 334 268 265 137
134 54 36 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2265 2265
Size: 9151
EcoO109I rG'GnC_Cy
Cuts at: 2101 2256 2257 2288 2302 3059 4325 4395 2101
Size: 155 1 31 14 757 1266 70 6857
Fragments arranged by size:
6857 1266 757 155 70 31 14 1
EcoRI G'AATT_C
Cuts at: 3191 3565 6460 6838 3191
Size: 374 2895 378 5504
Fragments arranged by size:
5504 2895 378 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3444 3444
Size: 9151
FspI TGC'GCA
Cuts at: 7657 7657
Size: 9151
HaeII r_GCGC'y
Cuts at: 1678 2144 2182 2224 2274 8298 1678
Size: 466 38 42 50 6024 2531
Fragments arranged by size:
6024 2531 466 50 42 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2844 2876 2900 2932 3037
Size: 32 135 32 1272 32 24 32 105
Cuts at: 3037 3069 3317 3349 3524 3556 4271 4303 5381
Size: 32 248 32 175 32 715 32 1078
Cuts at: 5381 5413 5433 5465 7792 7824 7866 7898 1373
Size: 32 20 32 2327 32 42 32 2626
Fragments arranged by size:
2626 2327 1272 1078 715 248 175 135
105 42 32 32 32 32 32 32
32 32 32 32 32 32 24 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 5266 5761 6495 6741 669
Size: 988 281 1524 1804 495 734 246 3079
Fragments arranged by size:
3079 1804 1524 988 734 495 281 246
HindIII A'AGCT_T
Cuts at: 748 2939 3559 5096 748
Size: 2191 620 1537 4803
Fragments arranged by size:
4803 2191 1537 620
HpaI GTT'AAC
Cuts at: 6741 6741
Size: 9151
KasI G'GCGC_C
Cuts at: 2140 2270 2140
Size: 130 9021
KpnI G_GTAC'C
Cuts at: 5473 6476 5473
Size: 1003 8148
MfeI C'AATT_G
Cuts at: 6750 6750
Size: 9151
MluI A'CGCG_T
Cuts at: 9027 9027
Size: 9151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4224 4850 5457 5694 8265 8449 1305
Size: 2919 626 607 237 2571 184 2007
Fragments arranged by size:
2919 2571 2007 626 607 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2344 10
Size: 54 1425 343 512 6817
Fragments arranged by size:
6817 1425 512 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3755 4229 4610 5420 6059 6223 6255
Size: 648 2589 474 381 810 639 164 32
Cuts at: 6255 7109 7468 7627 518
Size: 854 359 159 2042
Fragments arranged by size:
2589 2042 854 810 648 639 474 381
359 164 159 32
MspA1I CmG'CkG
Cuts at: 1606 2062 2173 2226 2284 3794 4937 5271 6074
Size: 456 111 53 58 1510 1143 334 803
Cuts at: 6074 6567 7244 8392 8637 1606
Size: 493 677 1148 245 2120
Fragments arranged by size:
2120 1510 1148 1143 803 677 493 456
334 245 111 58 53
NaeI GCC'GGC
Cuts at: 2082 2082
Size: 9151
NarI GG'CG_CC
Cuts at: 2141 2271 2141
Size: 130 9021
NcoI C'CATG_G
Cuts at: 513 1065 2004 4439 513
Size: 552 939 2435 5225
Fragments arranged by size:
5225 2435 939 552
NdeI CA'TA_TG
Cuts at: 387 6190 387
Size: 5803 3348
NgoMIV G'CCGG_C
Cuts at: 2080 2080
Size: 9151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9151
NotI GC'GGCC_GC
Cuts at: 6598 6598
Size: 9151
NsiI A_TGCA'T
Cuts at: 2997 4432 6252 2997
Size: 1435 1820 5896
Fragments arranged by size:
5896 1820 1435
NspI r_CATG'y
Cuts at: 1132 1229 2999 5964 6509 8054 1132
Size: 97 1770 2965 545 1545 2229
Fragments arranged by size:
2965 2229 1770 1545 545 97
PasI CC'CwG_GG
Cuts at: 1348 4551 1348
Size: 3203 5948
PciI A'CATG_T
Cuts at: 8050 8050
Size: 9151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3263 3694 4440 5797 1108
Size: 2155 431 746 1357 4462
Fragments arranged by size:
4462 2155 1357 746 431
PmeI GTTT'AAAC
Cuts at: 6455 6455
Size: 9151
PmlI CAC'GTG
Cuts at: 3581 3581
Size: 9151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7220 7252 8733 8765 7220
Size: 32 1481 32 7606
Fragments arranged by size:
7606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2288 3059 2101
Size: 187 771 8193
Fragments arranged by size:
8193 771 187
PshAI GACnn'nnGTC
Cuts at: 1613 3229 1613
Size: 1616 7535
PsiI TTA'TAA
Cuts at: 6721 6721
Size: 9151
PspOMI G'GGCC_C
Cuts at: 2228 2256 2301 2228
Size: 28 45 9078
Fragments arranged by size:
9078 45 28
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3993 4025 3993
Size: 32 9119
PstI C_TGCA'G
Cuts at: 830 2172 3142 3409 3793 4636 5342 6503 830
Size: 1342 970 267 384 843 706 1161 3478
Fragments arranged by size:
3478 1342 1161 970 843 706 384 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9151
PvuII CAG'CTG
Cuts at: 2173 4937 5271 2173
Size: 2764 334 6053
Fragments arranged by size:
6053 2764 334
SacI G_AGCT'C
Cuts at: 2969 4058 2969
Size: 1089 8062
SacII CC_GC'GG
Cuts at: 2227 6075 2227
Size: 3848 5303
SalI G'TCGA_C
Cuts at: 1936 6493 1936
Size: 4557 4594
SanDI GG'GwC_CC
Cuts at: 2101 2288 2101
Size: 187 8964
SapI GCTCTTCn'nnn_
Cuts at: 2447 2536 2447
Size: 89 9062
SbfI CC_TGCA'GG
Cuts at: 6503 6503
Size: 9151
ScaI AGT'ACT
Cuts at: 7399 9111 7399
Size: 1712 7439
SfcI C'TryA_G
Cuts at: 826 1045 2168 2251 2878 3138 3405 3789 4632
Size: 219 1123 83 627 260 267 384 843
Cuts at: 4632 4917 4932 5338 5646 6499 7634 8315 8506
Size: 285 15 406 308 853 1135 681 191
Cuts at: 8506 826
Size: 1471
Fragments arranged by size:
1471 1135 1123 853 843 681 627 406
384 308 285 267 260 219 191 83
15
SfoI GGC'GCC
Cuts at: 2142 2272 2142
Size: 130 9021
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9151
SgrAI Cr'CCGG_yG
Cuts at: 1700 2080 1700
Size: 380 8771
SmaI CCC'GGG
Cuts at: 1872 2092 2216 6478 1872
Size: 220 124 4262 4545
Fragments arranged by size:
4545 4262 220 124
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4640 7255 8156 8418 820
Size: 197 926 2697 2615 901 262 1553
Fragments arranged by size:
2697 2615 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6942 493
Size: 6449 2702
SpeI A'CTAG_T
Cuts at: 152 4972 152
Size: 4820 4331
SphI G_CATG'C
Cuts at: 1132 6509 1132
Size: 5377 3774
SspI AAT'ATT
Cuts at: 5 52 7075 5
Size: 47 7023 2081
Fragments arranged by size:
7023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9151
StyI C'CwwG_G
Cuts at: 513 1065 2004 3087 3597 3693 4439 6016 6097
Size: 552 939 1083 510 96 746 1577 81
Cuts at: 6097 6590 513
Size: 493 3074
Fragments arranged by size:
3074 1577 1083 939 746 552 510 493
96 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3364 3701 4234 7178 7195 7348 7533
Size: 1255 1784 337 533 2944 17 153 185
Cuts at: 7533 7978 325
Size: 445 1498
Fragments arranged by size:
2944 1784 1498 1255 533 445 337 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2930 3484 5115
Size: 274 80 33 51 1443 953 554 1631
Cuts at: 5115 6294 7397 9109 96
Size: 1179 1103 1712 138
Fragments arranged by size:
1712 1631 1443 1179 1103 953 554 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2624 2656 9111 9143 2624
Size: 32 6455 32 2632
Fragments arranged by size:
6455 2632 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3331 1316
Size: 2015 7136
XbaI T'CTAG_A
Cuts at: 6487 6487
Size: 9151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2862 2862
Size: 9151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9151
XmaI C'CCGG_G
Cuts at: 1870 2090 2214 6476 1870
Size: 220 124 4262 4545
Fragments arranged by size:
4545 4262 220 124
XmnI GAAnn'nnTTC
Cuts at: 2628 7280 2628
Size: 4652 4499
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6959 276
Size: 53 83 186 936 5425 2468
Fragments arranged by size:
5425 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BsiWI BsmBI BstZ17I EcoRV FseI
FspAI NruI PacI PfoI PspXI RsrII SexAI SfiI
SrfI SwaI