Restriction map for
pFN21AA1209
(Circular) (Six-base) MAPSORT of: pFN21AA1209.seq Check: 4115 from: 1 to: 9037
pFN21AA1209 9037 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6847 278
Size: 53 83 186 936 5311 2468
Fragments arranged by size:
5311 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6380 1937
Size: 4443 4594
Acc65I G'GTAC_C
Cuts at: 6358 6358
Size: 9037
AclI AA'CG_TT
Cuts at: 1563 7164 7537 1563
Size: 5601 373 3063
Fragments arranged by size:
5601 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2848 3310 3669 7100 8484 8752 1632
Size: 563 653 462 359 3431 1384 268 1917
Fragments arranged by size:
3431 1917 1384 653 563 462 359 268
AfeI AGC'GCT
Cuts at: 5698 5698
Size: 9037
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8840
AflIII A'CryG_T
Cuts at: 1203 3249 7936 8913 1203
Size: 2046 4687 977 1327
Fragments arranged by size:
4687 2046 1327 977
AgeI A'CCGG_T
Cuts at: 7868 7868
Size: 9037
AhdI GACnn_n'nnGTC
Cuts at: 5497 7766 5497
Size: 2269 6768
AleI CACnn'nnGTG
Cuts at: 1166 4886 1166
Size: 3720 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2058 2396 3305 6158 1184
Size: 874 338 909 2853 4063
Fragments arranged by size:
4063 2853 909 874 338
AlwNI CAG_nnn'CTG
Cuts at: 5603 5800 5803 6780 8352 5603
Size: 197 3 977 1572 6288
Fragments arranged by size:
6288 1572 977 197 3
ApaI G_GGCC'C
Cuts at: 5268 5268
Size: 9037
ApaLI G'TGCA_C
Cuts at: 7094 8250 7094
Size: 1156 7881
ApoI r'AATT_y
Cuts at: 1493 2541 2858 5340 5941 6305 6346 6572 6724
Size: 1048 317 2482 601 364 41 226 152
Cuts at: 6724 8833 1493
Size: 2109 1697
Fragments arranged by size:
2482 2109 1697 1048 601 364 317 226
152 41
AseI AT'TA_AT
Cuts at: 160 1032 7591 160
Size: 872 6559 1606
Fragments arranged by size:
6559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9037
AvaI C'yCGr_G
Cuts at: 1870 1943 6362 1870
Size: 73 4419 4545
Fragments arranged by size:
4545 4419 73
BamHI G'GATC_C
Cuts at: 6367 6734 6367
Size: 367 8670
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2473 4337 5276 6358
Size: 325 217 627 301 385 1864 939 1082
Cuts at: 6358 7813 618
Size: 1455 1842
Fragments arranged by size:
1864 1842 1455 1082 939 627 385 325
301 217
BanII G_rGCy'C
Cuts at: 1391 2190 2507 3766 4316 4422 5268 6356 1391
Size: 799 317 1259 550 106 846 1088 4072
Fragments arranged by size:
4072 1259 1088 846 799 550 317 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 3325 3626 3675 4737 4944 928
Size: 971 284 1142 301 49 1062 207 5021
Fragments arranged by size:
5021 1142 1062 971 301 284 207 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7228 7262 1903
Size: 34 5291 34 3678
Fragments arranged by size:
5291 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6929 8139 6929
Size: 1210 7827
BclI T'GATC_A
Cuts at: 1548 3738 5745 1548
Size: 2190 2007 4840
Fragments arranged by size:
4840 2190 2007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2301 4792 7648
Size: 107 122 71 631 605 629 2491 2856
Cuts at: 7648 136
Size: 1525
Fragments arranged by size:
2856 2491 1525 631 629 605 122 107
71
BglII A'GATC_T
Cuts at: 3889 5896 9032 3889
Size: 2007 3136 3894
Fragments arranged by size:
3894 3136 2007
BlpI GC'TnA_GC
Cuts at: 3320 4203 4820 6454 3320
Size: 883 617 1634 5903
Fragments arranged by size:
5903 1634 883 617
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2476 5268 5882 7098 8254 1790
Size: 301 385 2792 614 1216 1156 2573
Fragments arranged by size:
2792 2573 1216 1156 614 385 301
BmgBI CAC'GTC
Cuts at: 1318 4831 1318
Size: 3513 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2782 4623 5293
Size: 416 53 83 399 24 1365 1841 670
Cuts at: 5293 5719 6768 7726 442
Size: 426 1049 958 1753
Fragments arranged by size:
1841 1753 1365 1049 958 670 426 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9037
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4766 4890 4766
Size: 124 8913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 3449 3961 4514 4535 5198 5204 7697
Size: 1124 1086 512 553 21 663 6 2493
Cuts at: 7697 1239
Size: 2579
Fragments arranged by size:
2579 2493 1124 1086 663 553 512 21
6
Bpu10I CC'TnA_GC
Cuts at: 3456 4815 3456
Size: 1359 7678
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2151 2924 3179 5352 5515 7162 8027 8325 2151
Size: 773 255 2173 163 1647 865 298 2863
Fragments arranged by size:
2863 2173 1647 865 773 298 255 163
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3965 4737 7700 882
Size: 617 2466 772 2963 2219
Fragments arranged by size:
2963 2466 2219 772 617
BsaAI yAC'GTr
Cuts at: 493 1204 6828 493
Size: 711 5624 2702
Fragments arranged by size:
5624 2702 711
BsaBI GATnn'nnATC
Cuts at: 3855 9031 3855
Size: 5176 3861
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6844 7226 7861 275
Size: 53 83 186 936 5311 382 635 1451
Fragments arranged by size:
5311 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3084 7470 7868 8142 8289 8700 3084
Size: 4386 398 274 147 411 3421
Fragments arranged by size:
4386 3421 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4021 4051 1178
Size: 30 165 30 2618 30 6164
Fragments arranged by size:
6164 2618 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 3874 4118 4409 4868 1186
Size: 1318 1370 244 291 459 5355
Fragments arranged by size:
5355 1370 1318 459 291 244
BseYI C'CCAG_C
Cuts at: 3381 3675 3812 4016 4438 5355 8240 8736 3381
Size: 294 137 204 422 917 2885 496 3682
Fragments arranged by size:
3682 2885 917 496 422 294 204 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3392 4471 4781 5627 1163
Size: 573 1656 1079 310 846 4573
Fragments arranged by size:
4573 1656 1079 846 573 310
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2487 6487 7248 8276 1540
Size: 275 186 486 4000 761 1028 2301
Fragments arranged by size:
4000 2301 1028 761 486 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 3078 3766 4268 4481 4811 7098 7183 8254
Size: 888 688 502 213 330 2287 85 1071
Cuts at: 8254 2190
Size: 2973
Fragments arranged by size:
2973 2287 1071 888 688 502 330 213
85
BsmI GAATG_Cn'
Cuts at: 5437 6145 6548 6641 5437
Size: 708 403 93 7833
Fragments arranged by size:
7833 708 403 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3702 4754 3702
Size: 1052 7985
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2190 2476 2507 3078 3766 4268
Size: 399 301 99 286 31 571 688 502
Cuts at: 4268 4316 4422 4481 4811 5268 5882 6356 7098
Size: 48 106 59 330 457 614 474 742
Cuts at: 7098 7183 8254 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 688 614 571 502 474
457 399 330 301 286 106 99 85
59 48 31
BspEI T'CCGG_A
Cuts at: 8700 8700
Size: 9037
BspHI T'CATG_A
Cuts at: 6926 8656 6926
Size: 1730 7307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6392 844
Size: 5548 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3196 3319 3755 6924 1394
Size: 429 1373 123 436 3169 3507
Fragments arranged by size:
3507 3169 1373 436 429 123
BsrDI GCAATG_nn'
Cuts at: 65 1248 6140 7532 7706 65
Size: 1183 4892 1392 174 1396
Fragments arranged by size:
4892 1396 1392 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4977 5194 6178 7681 7868 1700
Size: 110 3167 217 984 1503 187 2869
Fragments arranged by size:
3167 2869 1503 984 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 2566 96
Size: 2470 6567
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9037
BssSI C'ACGA_G
Cuts at: 2500 3071 7097 8109 2500
Size: 571 4026 1012 3428
Fragments arranged by size:
4026 3428 1012 571
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 4032 5800 794
Size: 1301 1937 1768 4031
Fragments arranged by size:
4031 1937 1768 1301
BstBI TT'CG_AA
Cuts at: 7841 7841
Size: 9037
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9037
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 5420 2341
Size: 3079 5958
BstYI r'GATC_y
Cuts at: 1972 3668 3889 4426 5896 6367 6734 7119 7136
Size: 1696 221 537 1470 471 367 385 17
Cuts at: 7136 8588 8674 8686 9032 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1696 1470 1452 537 471 385 367
346 221 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3519 4523 5384 3519
Size: 1004 861 7172
Fragments arranged by size:
7172 1004 861
BtgI C'CryG_G
Cuts at: 513 1065 2004 2254 2400 2814 5413 513
Size: 552 939 250 146 414 2599 4137
Fragments arranged by size:
4137 2599 939 552 414 250 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8298
Fragments arranged by size:
8298 683 56
BtsI GCAGTG_nn'
Cuts at: 2456 4018 6553 7347 7367 2456
Size: 1562 2535 794 20 4126
Fragments arranged by size:
4126 2535 1562 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2796 2831 6081 6116 529
Size: 35 2232 35 3250 35 3450
Fragments arranged by size:
3450 3250 2232 35 35 35
DraI TTT'AAA
Cuts at: 2577 5163 6341 2577
Size: 2586 1178 5273
Fragments arranged by size:
5273 2586 1178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2207 8044 809
Size: 507 891 5837 1802
Fragments arranged by size:
5837 1802 891 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2496 2736 2853
Size: 54 1425 50 275 18 666 240 117
Cuts at: 2853 4300 5028 5196 6484 7373 9002 8
Size: 1447 728 168 1288 889 1629 43
Fragments arranged by size:
1629 1447 1425 1288 889 728 666 275
240 168 117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6484 1537
Size: 275 4672 4090
Fragments arranged by size:
4672 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2245 2763 3209 3215 3233 4886 5241
Size: 671 221 518 446 6 18 1653 355
Cuts at: 5241 5580 6967 1353
Size: 339 1387 3423
Fragments arranged by size:
3423 1653 1387 671 518 446 355 339
221 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 7624 7998 8144 387
Size: 1064 138 452 5583 374 146 1280
Fragments arranged by size:
5583 1280 1064 452 374 146 138
EcoICRI GAG'CTC
Cuts at: 2188 3764 2188
Size: 1576 7461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2363 2848 3310 3449 3669 3961
Size: 393 563 168 485 462 139 220 292
Cuts at: 3961 4514 4535 5198 5204 7100 7697 8484 8752
Size: 553 21 663 6 1896 597 787 268
Cuts at: 8752 1239
Size: 1524
Fragments arranged by size:
1896 1524 787 663 597 563 553 485
462 393 292 268 220 168 139 21
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2981 3522 4044 5595 2981
Size: 541 522 1551 6423
Fragments arranged by size:
6423 1551 541 522
EcoO109I rG'GnC_Cy
Cuts at: 2907 5264 5721 2907
Size: 2357 457 6223
Fragments arranged by size:
6223 2357 457
EcoRI G'AATT_C
Cuts at: 6346 6724 6346
Size: 378 8659
EcoRV GAT'ATC
Cuts at: 2677 5507 2677
Size: 2830 6207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3491 3491
Size: 9037
FspI TGC'GCA
Cuts at: 7543 7543
Size: 9037
HaeII r_GCGC'y
Cuts at: 1678 4840 5700 8184 1678
Size: 3162 860 2484 2531
Fragments arranged by size:
3162 2531 2484 860
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3653 3685 3700 3732 3881
Size: 32 135 32 2081 32 15 32 149
Cuts at: 3881 3913 4418 4450 4658 4690 5483 5515 7678
Size: 32 505 32 208 32 793 32 2163
Cuts at: 7678 7710 7752 7784 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2163 2081 793 505 208 149 135
42 32 32 32 32 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4137 6184 6381 6627 669
Size: 988 281 2199 2047 197 246 3079
Fragments arranged by size:
3079 2199 2047 988 281 246 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9037
HpaI GTT'AAC
Cuts at: 6184 6627 6184
Size: 443 8594
KpnI G_GTAC'C
Cuts at: 6362 6362
Size: 9037
MfeI C'AATT_G
Cuts at: 5546 6636 5546
Size: 1090 7947
MluI A'CGCG_T
Cuts at: 8913 8913
Size: 9037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5663 6030 6036 8151 8335 1305
Size: 4358 367 6 2115 184 2007
Fragments arranged by size:
4358 2115 2007 367 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2738 2855 4302 5030 10
Size: 54 1425 343 906 117 1447 728 4017
Fragments arranged by size:
4017 1447 1425 906 728 343 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3051 3293 4161 4886 5418 6995 7354
Size: 648 1885 242 868 725 532 1577 359
Cuts at: 7354 7513 518
Size: 159 2042
Fragments arranged by size:
2042 1885 1577 868 725 648 532 359
242 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2932 3679 3942 5800 6132 6453 7130
Size: 511 815 747 263 1858 332 321 677
Cuts at: 7130 8278 8523 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1858 1148 815 747 677 511 332
321 263 245
NaeI GCC'GGC
Cuts at: 5196 5196
Size: 9037
NcoI C'CATG_G
Cuts at: 513 1065 2004 2254 5413 513
Size: 552 939 250 3159 4137
Fragments arranged by size:
4137 3159 939 552 250
NdeI CA'TA_TG
Cuts at: 387 3948 387
Size: 3561 5476
NgoMIV G'CCGG_C
Cuts at: 5194 5194
Size: 9037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9037
NotI GC'GGCC_GC
Cuts at: 6484 6484
Size: 9037
NspI r_CATG'y
Cuts at: 1132 1229 4035 6395 7940 1132
Size: 97 2806 2360 1545 2229
Fragments arranged by size:
2806 2360 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2632 1348
Size: 1284 7753
PciI A'CATG_T
Cuts at: 7936 7936
Size: 9037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9037
PmeI GTTT'AAAC
Cuts at: 6341 6341
Size: 9037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7106 7138 8619 8651 7106
Size: 32 1481 32 7492
Fragments arranged by size:
7492 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2907 5721 2907
Size: 2814 6223
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9037
PsiI TTA'TAA
Cuts at: 6607 6607
Size: 9037
PspOMI G'GGCC_C
Cuts at: 5264 5264
Size: 9037
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2112 2144 2112
Size: 32 9005
PstI C_TGCA'G
Cuts at: 830 2385 3360 4077 6131 6389 830
Size: 1555 975 717 2054 258 3478
Fragments arranged by size:
3478 2054 1555 975 717 258
PvuI CG_AT'CG
Cuts at: 2001 2487 2001
Size: 486 8551
PvuII CAG'CTG
Cuts at: 2117 3679 3942 5800 2117
Size: 1562 263 1858 5354
Fragments arranged by size:
5354 1858 1562 263
RsrII CG'GwC_CG
Cuts at: 3082 3082
Size: 9037
SacI G_AGCT'C
Cuts at: 2190 3766 2190
Size: 1576 7461
SalI G'TCGA_C
Cuts at: 1936 6379 1936
Size: 4443 4594
SapI GCTCTTCn'nnn_
Cuts at: 3209 4886 3209
Size: 1677 7360
SbfI CC_TGCA'GG
Cuts at: 6389 6389
Size: 9037
ScaI AGT'ACT
Cuts at: 5536 7285 8997 5536
Size: 1749 1712 5576
Fragments arranged by size:
5576 1749 1712
SfcI C'TryA_G
Cuts at: 826 1045 2381 3018 3356 4073 5446 6127 6385
Size: 219 1336 637 338 717 1373 681 258
Cuts at: 6385 7520 8201 8392 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1373 1336 1135 717 681 681 637
338 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9037
SmaI CCC'GGG
Cuts at: 1872 6364 1872
Size: 4492 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2166 2939 3158 5367 5494 7141
Size: 197 926 223 773 219 2209 127 1647
Cuts at: 7141 8042 8304 820
Size: 901 262 1553
Fragments arranged by size:
2209 1647 1553 926 901 773 262 223
219 197 127
SnaBI TAC'GTA
Cuts at: 493 6828 493
Size: 6335 2702
SpeI A'CTAG_T
Cuts at: 152 4052 152
Size: 3900 5137
SphI G_CATG'C
Cuts at: 1132 6395 1132
Size: 5263 3774
SspI AAT'ATT
Cuts at: 5 52 6283 6961 5
Size: 47 6231 678 2081
Fragments arranged by size:
6231 2081 678 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9037
StyI C'CwwG_G
Cuts at: 513 1065 2004 2254 2807 5413 5676 6476 513
Size: 552 939 250 553 2606 263 800 3074
Fragments arranged by size:
3074 2606 939 800 553 552 263 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2217 2455 2491 2654 3797 7064 7081
Size: 1255 637 238 36 163 1143 3267 17
Cuts at: 7081 7234 7419 7864 325
Size: 153 185 445 1498
Fragments arranged by size:
3267 1498 1255 1143 637 445 238 185
163 153 36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2566 5127 5534
Size: 274 80 33 51 1443 589 2561 407
Cuts at: 5534 7283 8995 96
Size: 1749 1712 138
Fragments arranged by size:
2561 1749 1712 1443 589 407 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2044 2076 2788 2820 3627 3659 4021 4053 8997
Size: 32 712 32 807 32 362 32 4944
Cuts at: 8997 9029 2044
Size: 32 2052
Fragments arranged by size:
4944 2052 807 712 362 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4157 1316
Size: 2841 6196
XbaI T'CTAG_A
Cuts at: 3656 6373 3656
Size: 2717 6320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5411 5417 5411
Size: 6 9031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9037
XmaI C'CCGG_G
Cuts at: 1870 6362 1870
Size: 4492 4545
XmnI GAAnn'nnTTC
Cuts at: 6306 7166 6306
Size: 860 8177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6845 276
Size: 53 83 186 936 5311 2468
Fragments arranged by size:
5311 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I DraIII FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI SacII SanDI SexAI
SfiI SfoI SrfI SwaI