Restriction map for
pFN21AA1191
(Circular) (Six-base) MAPSORT of: pFN21AA1191.seq Check: 1444 from: 1 to: 5620
pFN21AA1191 5620 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2233 2963 1937
Size: 296 730 4594
Fragments arranged by size:
4594 730 296
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 2817 3683 5067 5335 1632
Size: 1049 136 866 1384 268 1917
Fragments arranged by size:
1917 1384 1049 866 268 136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 2574 3363 4935 2574
Size: 789 1572 3259
Fragments arranged by size:
3259 1572 789
ApaI G_GGCC'C
Cuts at: 2872 2872
Size: 5620
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2323 2929 3155 3307 5416 1493
Size: 830 606 226 152 2109 1697
Fragments arranged by size:
2109 1697 830 606 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2945 1870
Size: 73 171 831 4545
Fragments arranged by size:
4545 831 171 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2746 2872 2939 1391
Size: 1355 126 67 4072
Fragments arranged by size:
4072 1355 126 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2105 2139 3811 3845 1903
Size: 34 168 34 1672 34 3678
Fragments arranged by size:
3678 1672 168 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 2331 1548
Size: 783 4837
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2729 4231 136
Size: 107 122 71 631 605 1057 1502 1525
Fragments arranged by size:
1525 1502 1057 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2779 2872 3681 4837 1790
Size: 989 93 809 1156 2573
Fragments arranged by size:
2573 1156 989 809 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 2515 2831
Size: 416 53 83 399 24 865 233 316
Cuts at: 2831 3351 4309 442
Size: 520 958 1753
Fragments arranged by size:
1753 958 865 520 416 399 316 233
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4280 1239
Size: 3041 2579
Bpu10I CC'TnA_GC
Cuts at: 2556 2556
Size: 5620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 3745 4610 4908 2057
Size: 1688 865 298 2769
Fragments arranged by size:
2769 1688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2423 4283 882
Size: 617 924 1860 2219
Fragments arranged by size:
2219 1860 924 617
BsaAI yAC'GTr
Cuts at: 493 1204 2774 3411 493
Size: 711 1570 637 2702
Fragments arranged by size:
2702 1570 711 637
BsaBI GATnn'nnATC
Cuts at: 2831 5614 2831
Size: 2783 2837
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2125 2155 2420 2450 1178
Size: 30 165 30 722 30 265 30 4348
Fragments arranged by size:
4348 722 265 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2130 2441 2754 1186
Size: 941 3 311 313 4052
Fragments arranged by size:
4052 941 313 311 3
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2904 3681 3766 4837 2904
Size: 777 85 1071 3687
Fragments arranged by size:
3687 1071 777 85
BsmI GAATG_Cn'
Cuts at: 2355 3131 3224 2355
Size: 776 93 4751
Fragments arranged by size:
4751 776 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2746 2779 2872 2904 2939 3681 3766
Size: 399 956 33 93 32 35 742 85
Cuts at: 3766 4837 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 956 742 399 93 85 35
33 32
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 2319 3509 5239 2319
Size: 1190 1730 2700
Fragments arranged by size:
2700 1730 1190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 2791
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2690 2800 2950 3317 3702 3719 5171 5257
Size: 718 110 150 367 385 17 1452 86
Cuts at: 5257 5269 5615 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 150 110
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2625 2864 513
Size: 552 939 621 239 3269
Fragments arranged by size:
3269 939 621 552 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2448 3067 3956
Size: 54 1425 50 275 18 618 619 889
Cuts at: 3956 5585 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 619 618 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2493 2554 3550 1353
Size: 1140 61 996 3423
Fragments arranged by size:
3423 1140 996 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2681 2817 3683 4280 5067 5335 1239
Size: 393 1049 136 866 597 787 268 1524
Fragments arranged by size:
1524 1049 866 787 597 393 268 136
EcoNI CCTnn'n_nnAGG
Cuts at: 2207 2207
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2119 2284 2413 2119
Size: 165 129 5326
Fragments arranged by size:
5326 165 129
EcoRI G'AATT_C
Cuts at: 2323 2929 3307 2323
Size: 606 378 4636
Fragments arranged by size:
4636 606 378
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 4261 4293 4335
Size: 32 135 32 553 32 2104 32 42
Cuts at: 4335 4367 1373
Size: 32 2626
Fragments arranged by size:
2626 2104 553 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3578 3937 4096 518
Size: 648 1671 741 359 159 2042
Fragments arranged by size:
2042 1671 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2625 513
Size: 552 939 621 3508
Fragments arranged by size:
3508 939 621 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2568 1348
Size: 1220 4400
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2574 1108
Size: 1466 4154
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2284 2119
Size: 165 5455
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PspOMI G'GGCC_C
Cuts at: 2868 2868
Size: 5620
PspXI vC'TCGA_Gb
Cuts at: 2114 2114
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2968 4103 4784 4975 826
Size: 219 1923 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2114 3724 4625 4887 820
Size: 197 926 93 78 1610 901 262 1553
Fragments arranged by size:
1610 1553 926 901 262 197 93 78
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2732 1517
Size: 1215 4405
StyI C'CwwG_G
Cuts at: 513 1065 2004 2165 2208 2625 3059 513
Size: 552 939 161 43 417 434 3074
Fragments arranged by size:
3074 939 552 434 417 161 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2701 2741 2923 3647 3664 3817 4002
Size: 1255 1121 40 182 724 17 153 185
Cuts at: 4002 4447 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1121 724 445 185 182 153
40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 2254 2282 1316
Size: 938 28 4654
Fragments arranged by size:
4654 938 28
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2272
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 2114 1943
Size: 171 5449
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 2355 3749 2355
Size: 1394 4226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI