Restriction map for
pFN21AA1190
(Circular) (Six-base) MAPSORT of: pFN21AA1190.seq Check: 4667 from: 1 to: 6946
pFN21AA1190 6946 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3659 4188 3659
Size: 529 6417
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4756 278
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4289 1937
Size: 2352 4594
Acc65I G'GTAC_C
Cuts at: 3170 3351 4267 3170
Size: 181 916 5849
Fragments arranged by size:
5849 916 181
AclI AA'CG_TT
Cuts at: 1563 4245 5073 5446 1563
Size: 2682 828 373 3063
Fragments arranged by size:
3063 2682 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3204 5009 6393 6661 1632
Size: 1572 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1572 1384 268
AfeI AGC'GCT
Cuts at: 3416 3755 4007 3416
Size: 339 252 6355
Fragments arranged by size:
6355 339 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 2742 3485 3993 5845 6822 1203
Size: 1539 743 508 1852 977 1327
Fragments arranged by size:
1852 1539 1327 977 743 508
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 5675 5675
Size: 6946
AleI CACnn'nnGTG
Cuts at: 1166 2100 1166
Size: 934 6012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3639 1184
Size: 2455 4491
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 6914
AlwNI CAG_nnn'CTG
Cuts at: 2522 2540 2737 3266 4162 4689 6261 2522
Size: 18 197 529 896 527 1572 3207
Fragments arranged by size:
3207 1572 896 529 527 197 18
ApaI G_GGCC'C
Cuts at: 2483 2613 2483
Size: 130 6816
ApaLI G'TGCA_C
Cuts at: 3539 3698 5003 6159 3539
Size: 159 1305 1156 4326
Fragments arranged by size:
4326 1305 1156 159
ApoI r'AATT_y
Cuts at: 1493 4255 4481 4633 6742 1493
Size: 2762 226 152 2109 1697
Fragments arranged by size:
2762 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5500 160
Size: 872 4468 1606
Fragments arranged by size:
4468 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 3147 3249 4144 4271 1870
Size: 73 1204 102 895 127 4545
Fragments arranged by size:
4545 1204 895 127 102 73
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2191 2675 2771 3162
Size: 325 217 627 281 123 484 96 391
Cuts at: 3162 3170 3351 3951 4267 5722 618
Size: 8 181 600 316 1455 1842
Fragments arranged by size:
1842 1455 627 600 484 391 325 316
281 217 181 123 96 8
BanII G_rGCy'C
Cuts at: 1391 2483 2613 3038 4265 1391
Size: 1092 130 425 1227 4072
Fragments arranged by size:
4072 1227 1092 425 130
BbeI G_GCGC'C
Cuts at: 3166 3166
Size: 6946
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3881 928
Size: 971 1982 3993
Fragments arranged by size:
3993 1982 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5137 5171 1903
Size: 34 3200 34 3678
Fragments arranged by size:
3678 3200 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4838 6048 4838
Size: 1210 5736
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6946
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2384 5557 136
Size: 107 122 71 631 605 712 3173 1525
Fragments arranged by size:
3173 1525 712 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2463 3393 3573 3909 6941 2463
Size: 930 180 336 3032 2468
Fragments arranged by size:
3032 2468 930 336 180
BlpI GC'TnA_GC
Cuts at: 2273 4363 2273
Size: 2090 4856
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2366 2483 2613 2970 3329 3543 3702
Size: 283 293 117 130 357 359 214 159
Cuts at: 3702 5007 6163 1790
Size: 1305 1156 2573
Fragments arranged by size:
2573 1305 1156 359 357 293 283 214
159 130 117
BmgBI CAC'GTC
Cuts at: 1318 2053 3994 1318
Size: 735 1941 4270
Fragments arranged by size:
4270 1941 735
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4677 5635 442
Size: 416 53 83 399 24 3260 958 1753
Fragments arranged by size:
3260 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2111 1056
Size: 1055 5891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2385 2654 3249 3314 3377 3446 5606
Size: 962 184 269 595 65 63 69 2160
Cuts at: 5606 1239
Size: 2579
Fragments arranged by size:
2579 2160 962 595 269 184 69 65
63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3677 4013 5071 5936 6234 3677
Size: 336 1058 865 298 4389
Fragments arranged by size:
4389 1058 865 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3650 5609 882
Size: 617 2151 1959 2219
Fragments arranged by size:
2219 2151 1959 617
BsaAI yAC'GTr
Cuts at: 493 1204 2743 3607 4737 493
Size: 711 1539 864 1130 2702
Fragments arranged by size:
2702 1539 1130 864 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2334 3163 4083 4753
Size: 53 83 186 936 801 829 920 670
Cuts at: 4753 5135 5770 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 920 829 801 670 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5379 5777 6051 6198 6609 5379
Size: 398 274 147 411 5716
Fragments arranged by size:
5716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3713 3743 1178
Size: 30 165 30 2310 30 4381
Fragments arranged by size:
4381 2310 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2591 2612 2852 2855 2858 2870 2873 2876
Size: 1405 21 240 3 3 12 3 3
Cuts at: 2876 2879 2921 2924 2927 2930 2969 2972 3005
Size: 3 42 3 3 3 39 3 33
Cuts at: 3005 3008 3017 3056 3059 3062 3071 3311 1186
Size: 3 9 39 3 3 9 240 4821
Fragments arranged by size:
4821 1405 240 240 42 39 39 33
21 12 9 9 3 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2073 2379 2559 2614 2785 3068 3191 3274 4113
Size: 306 180 55 171 283 123 83 839
Cuts at: 4113 6149 6645 2073
Size: 2036 496 2374
Fragments arranged by size:
2374 2036 839 496 306 283 180 171
123 83 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2380 2723 3428 1163
Size: 573 644 343 705 4681
Fragments arranged by size:
4681 705 644 573 343
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 3038 3543 3702 3870 5007 5092 6163 2274
Size: 764 505 159 168 1137 85 1071 3057
Fragments arranged by size:
3057 1137 1071 764 505 168 159 85
BsmI GAATG_Cn'
Cuts at: 4457 4550 4457
Size: 93 6853
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2274 2366 2483 2613 2970 3038
Size: 399 283 201 92 117 130 357 68
Cuts at: 3038 3329 3543 3702 3870 4265 5007 5092 6163
Size: 291 214 159 168 395 742 85 1071
Cuts at: 6163 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 395 357 291 283
214 201 168 159 130 117 92 85
68
BspEI T'CCGG_A
Cuts at: 6609 6609
Size: 6946
BspHI T'CATG_A
Cuts at: 4835 6565 4835
Size: 1730 5216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3659 3668 4188 4301 844
Size: 2815 9 520 113 3489
Fragments arranged by size:
3489 2815 520 113 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2340 4198 4833 1394
Size: 429 517 1858 635 3507
Fragments arranged by size:
3507 1858 635 517 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2285 3754 5441 5615 65
Size: 1183 1037 1469 1687 174 1396
Fragments arranged by size:
1687 1469 1396 1183 1037 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2383 2603 3933 3954 4073 5590 5777
Size: 110 573 220 1330 21 119 1517 187
Cuts at: 5777 1700
Size: 2869
Fragments arranged by size:
2869 1517 1330 573 220 187 119 110
21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6946
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 2264 3330 5006 6018 2264
Size: 1066 1676 1012 3192
Fragments arranged by size:
3192 1676 1066 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2522 3706 4132 794
Size: 1728 1184 426 3608
Fragments arranged by size:
3608 1728 1184 426
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6946
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2242
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 2211 2463 3393 3573 3909 4276 4643 5028
Size: 239 252 930 180 336 367 367 385
Cuts at: 5028 5045 6497 6583 6595 6941 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 930 385 367 367 346 336
252 239 180 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2748 2793 3086 3597 513
Size: 552 939 744 45 293 511 3862
Fragments arranged by size:
3862 939 744 552 511 293 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2290 487
Size: 683 56 1064 5143
Fragments arranged by size:
5143 1064 683 56
BtsI GCAGTG_nn'
Cuts at: 2735 2902 3704 4130 4209 4462 5256 5276 2735
Size: 167 802 426 79 253 794 20 4405
Fragments arranged by size:
4405 802 794 426 253 167 79 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6911
DraI TTT'AAA
Cuts at: 4250 4250
Size: 6946
DraIII CAC_nnn'GTG
Cuts at: 2198 2269 2198
Size: 71 6875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5953 809
Size: 507 4637 1802
Fragments arranged by size:
4637 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 3135 3802
Size: 54 1425 50 275 18 423 882 667
Cuts at: 3802 4000 4071 4393 5282 6911 8
Size: 198 71 322 889 1629 43
Fragments arranged by size:
1629 1425 889 882 667 423 322 275
198 71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2144 2640 2652 2868 2874 2916 2937
Size: 683 108 496 12 216 6 42 21
Cuts at: 2937 2946 2976 2982 2994 3203 4205 4876 1353
Size: 9 30 6 12 209 1002 671 3423
Fragments arranged by size:
3423 1002 683 671 496 216 209 108
42 30 21 12 12 9 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5533 5907 6053 387
Size: 1064 138 3944 374 146 1280
Fragments arranged by size:
3944 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3036 3036
Size: 6946
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2385 2654 3204 3249 3314 3377
Size: 393 569 184 269 550 45 65 63
Cuts at: 3377 3446 5009 5606 6393 6661 1239
Size: 69 1563 597 787 268 1524
Fragments arranged by size:
1563 1524 787 597 569 550 393 269
268 184 69 65 63 45
EcoO109I rG'GnC_Cy
Cuts at: 2479 2480 2610 3011 2479
Size: 1 130 401 6414
Fragments arranged by size:
6414 401 130 1
EcoRI G'AATT_C
Cuts at: 4255 4633 4255
Size: 378 6568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3874 3874
Size: 6946
FspI TGC'GCA
Cuts at: 5452 5452
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 2029 2280 3166 3418 3757 4009 6093 1678
Size: 351 251 886 252 339 252 2084 2531
Fragments arranged by size:
2531 2084 886 351 339 252 252 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 2477 2509 5587
Size: 32 135 32 752 32 121 32 3078
Cuts at: 5587 5619 5661 5693 1373
Size: 32 42 32 2626
Fragments arranged by size:
3078 2626 752 135 121 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2248 4290 4536 669
Size: 988 281 310 2042 246 3079
Fragments arranged by size:
3079 2042 988 310 281 246
HindIII A'AGCT_T
Cuts at: 748 3495 3506 748
Size: 2747 11 4188
Fragments arranged by size:
4188 2747 11
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KasI G'GCGC_C
Cuts at: 3162 3162
Size: 6946
KpnI G_GTAC'C
Cuts at: 3174 3355 4271 3174
Size: 181 916 5849
Fragments arranged by size:
5849 916 181
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2631 4044 6060 6244 1305
Size: 1326 1413 2016 184 2007
Fragments arranged by size:
2016 2007 1413 1326 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3137 3804 4002 10
Size: 54 1425 343 1305 667 198 2954
Fragments arranged by size:
2954 1425 1305 667 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 3369 3371 4904 5263 5422 518
Size: 648 934 1269 2 1533 359 159 2042
Fragments arranged by size:
2042 1533 1269 934 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2158 2394 2405 3348 3775 4091 4362 5039
Size: 552 236 11 943 427 316 271 677
Cuts at: 5039 6187 6432 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 943 677 552 427 316 271
245 236 11
NaeI GCC'GGC
Cuts at: 2385 3935 4075 2385
Size: 1550 140 5256
Fragments arranged by size:
5256 1550 140
NarI GG'CG_CC
Cuts at: 3163 3163
Size: 6946
NcoI C'CATG_G
Cuts at: 513 1065 2004 3597 513
Size: 552 939 1593 3862
Fragments arranged by size:
3862 1593 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6946
NgoMIV G'CCGG_C
Cuts at: 2383 3933 4073 2383
Size: 1550 140 5256
Fragments arranged by size:
5256 1550 140
NheI G'CTAG_C
Cuts at: 1052 2107 1052
Size: 1055 5891
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 3391 3489 3643 3688 4027 4304 5849
Size: 97 2162 98 154 45 339 277 1545
Cuts at: 5849 1132
Size: 2229
Fragments arranged by size:
2229 2162 1545 339 277 154 98 97
45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6946
PciI A'CATG_T
Cuts at: 3485 5845 3485
Size: 2360 4586
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3227 4069 1108
Size: 2119 842 3985
Fragments arranged by size:
3985 2119 842
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PmlI CAC'GTG
Cuts at: 2743 3607 2743
Size: 864 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3572 3577 3604 3713 3745 5015 5047 6528
Size: 27 5 27 109 32 1270 32 1481
Cuts at: 6528 6560 3545
Size: 32 3931
Fragments arranged by size:
3931 1481 1270 109 32 32 32 27
27 5
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 4516 4516
Size: 6946
PspOMI G'GGCC_C
Cuts at: 2479 2609 2479
Size: 130 6816
PstI C_TGCA'G
Cuts at: 830 2163 2208 2295 2823 3711 4298 830
Size: 1333 45 87 528 888 587 3478
Fragments arranged by size:
3478 1333 888 587 528 87 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 2158 2394 2405 3775 2158
Size: 236 11 1370 5329
Fragments arranged by size:
5329 1370 236 11
SacI G_AGCT'C
Cuts at: 3038 3038
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SapI GCTCTTCn'nnn_
Cuts at: 2652 4205 2652
Size: 1553 5393
SbfI CC_TGCA'GG
Cuts at: 4298 4298
Size: 6946
ScaI AGT'ACT
Cuts at: 3857 5194 6906 3857
Size: 1337 1712 3897
Fragments arranged by size:
3897 1712 1337
SexAI A'CCwGG_T
Cuts at: 2194 2353 2194
Size: 159 6787
SfcI C'TryA_G
Cuts at: 826 1045 2116 2159 2204 2291 2680 2819 3707
Size: 219 1071 43 45 87 389 139 888
Cuts at: 3707 4294 5429 6110 6301 826
Size: 587 1135 681 191 1471
Fragments arranged by size:
1471 1135 1071 888 681 587 389 219
191 139 87 45 43
SfoI GGC'GCC
Cuts at: 3164 3164
Size: 6946
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 3954 1700
Size: 2254 4692
SmaI CCC'GGG
Cuts at: 1872 3149 4146 4273 1872
Size: 1277 997 127 4545
Fragments arranged by size:
4545 1277 997 127
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3692 4028 5050 5951 6213 820
Size: 197 926 1749 336 1022 901 262 1553
Fragments arranged by size:
1749 1553 1022 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 3391 4304 1132
Size: 2259 913 3774
Fragments arranged by size:
3774 2259 913
SspI AAT'ATT
Cuts at: 5 52 4870 5
Size: 47 4818 2081
Fragments arranged by size:
4818 2081 47
StuI AGG'CCT
Cuts at: 1517 2454 4101 1517
Size: 937 1647 4362
Fragments arranged by size:
4362 1647 937
StyI C'CwwG_G
Cuts at: 513 1065 2004 3282 3597 3633 4385 513
Size: 552 939 1278 315 36 752 3074
Fragments arranged by size:
3074 1278 939 752 552 315 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 4973 4990 5143 5328 5773 325
Size: 1255 579 2814 17 153 185 445 1498
Fragments arranged by size:
2814 1498 1255 579 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3762 3855 5192
Size: 274 80 33 51 1443 1785 93 1337
Cuts at: 5192 6904 96
Size: 1712 138
Fragments arranged by size:
1785 1712 1443 1337 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2512 2544 2755 2787 6906 6938 2512
Size: 32 211 32 4119 32 2520
Fragments arranged by size:
4119 2520 211 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4082 1316
Size: 2766 4180
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 3271 3289 2797
Size: 474 18 6454
Fragments arranged by size:
6454 474 18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 3147 4144 4271 1870
Size: 1277 997 127 4545
Fragments arranged by size:
4545 1277 997 127
XmnI GAAnn'nnTTC
Cuts at: 3560 3587 3728 4049 5075 3560
Size: 27 141 321 1026 5431
Fragments arranged by size:
5431 1026 321 141 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4754 276
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI
NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII
SanDI SfiI SrfI SwaI