Restriction map for
pFN21AA1182
(Circular) (Six-base) MAPSORT of: pFN21AA1182.seq Check: 496 from: 1 to: 6445
pFN21AA1182 6445 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2370 2881 2976 3402 3543 2370
Size: 511 95 426 141 5272
Fragments arranged by size:
5272 511 426 141 95
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 1937
Size: 1851 4594
Acc65I G'GTAC_C
Cuts at: 3766 3766
Size: 6445
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2120 2408 2518 2519 4508 5892 6160
Size: 440 48 288 110 1 1989 1384 268
Cuts at: 6160 1632
Size: 1917
Fragments arranged by size:
1989 1917 1384 440 288 268 110 48
1
AfeI AGC'GCT
Cuts at: 2527 2527
Size: 6445
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6445
AlwNI CAG_nnn'CTG
Cuts at: 2026 2614 2668 2896 2977 4188 5760 2026
Size: 588 54 228 81 1211 1572 2711
Fragments arranged by size:
2711 1572 1211 588 228 81 54
ApaLI G'TGCA_C
Cuts at: 2699 3503 4502 5658 2699
Size: 804 999 1156 3486
Fragments arranged by size:
3486 1156 999 804
ApoI r'AATT_y
Cuts at: 1493 3428 3754 3980 4132 6241 1493
Size: 1935 326 226 152 2109 1697
Fragments arranged by size:
2109 1935 1697 326 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 3341 3770 1870
Size: 73 1398 429 4545
Fragments arranged by size:
4545 1398 429 73
BamHI G'GATC_C
Cuts at: 3775 4142 3775
Size: 367 6078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2589 2796 2964 3766 5221
Size: 325 217 627 802 207 168 802 1455
Cuts at: 5221 618
Size: 1842
Fragments arranged by size:
1842 1455 802 802 627 325 217 207
168
BanII G_rGCy'C
Cuts at: 1391 2547 3093 3111 3764 1391
Size: 1156 546 18 653 4072
Fragments arranged by size:
4072 1156 653 546 18
BbeI G_GCGC'C
Cuts at: 2593 2593
Size: 6445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 3073 928
Size: 971 393 781 4300
Fragments arranged by size:
4300 971 781 393
BbvCI CC'TCA_GC
Cuts at: 2262 3189 2262
Size: 927 5518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3413 3442 3447 3476 4636 4670 1903
Size: 34 1476 29 5 29 1160 34 3678
Fragments arranged by size:
3678 1476 1160 34 34 29 29 5
BciVI GTATCCnnnnn_n'
Cuts at: 4337 5547 4337
Size: 1210 5235
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5056 136
Size: 107 122 71 631 605 3384 1525
Fragments arranged by size:
3384 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2448 6440 2448
Size: 3992 2453
BlpI GC'TnA_GC
Cuts at: 2721 3862 2721
Size: 1141 5304
Bme1580I G_kGCm'C
Cuts at: 1790 2703 3507 3729 4506 5662 1790
Size: 913 804 222 777 1156 2573
Fragments arranged by size:
2573 1156 913 804 777 222
BmgBI CAC'GTC
Cuts at: 1318 2752 1318
Size: 1434 5011
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3197 4176 5134
Size: 416 53 83 399 24 1780 979 958
Cuts at: 5134 442
Size: 1753
Fragments arranged by size:
1780 1753 979 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2325 3643 2325
Size: 1318 5127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2246 2315 2534 3584 3605 3725 5105
Size: 962 45 69 219 1050 21 120 1380
Cuts at: 5105 1239
Size: 2579
Fragments arranged by size:
2579 1380 1050 962 219 120 69 45
21
Bpu10I CC'TnA_GC
Cuts at: 2262 3189 2262
Size: 927 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 3458 4570 5435 5733 2069
Size: 1389 1112 865 298 2781
Fragments arranged by size:
2781 1389 1112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 3347 5108 882
Size: 617 552 1296 1761 2219
Fragments arranged by size:
2219 1761 1296 617 552
BsaAI yAC'GTr
Cuts at: 493 1204 4236 493
Size: 711 3032 2702
Fragments arranged by size:
3032 2702 711
BsaBI GATnn'nnATC
Cuts at: 6439 6439
Size: 6445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2171 2590 2805 2871
Size: 53 83 186 936 638 419 215 66
Cuts at: 2871 3695 4252 4634 5269 275
Size: 824 557 382 635 1451
Fragments arranged by size:
1451 936 824 638 635 557 419 382
215 186 83 66 53
BsaWI w'CCGG_w
Cuts at: 2374 3663 4878 5276 5550 5697 6108 2374
Size: 1289 1215 398 274 147 411 2711
Fragments arranged by size:
2711 1289 1215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 3469 3499 3597
Size: 30 165 30 816 30 1220 30 98
Cuts at: 3597 3627 1178
Size: 30 3996
Fragments arranged by size:
3996 1220 816 165 98 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3206 3317 3515 1186
Size: 2020 111 198 4116
Fragments arranged by size:
4116 2020 198 111
BseYI C'CCAG_C
Cuts at: 2896 3043 3630 3671 3719 5648 6144 2896
Size: 147 587 41 48 1929 496 3197
Fragments arranged by size:
3197 1929 587 496 147 48 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2056 2147 2540 2627 2692 2723 2749
Size: 573 320 91 393 87 65 31 26
Cuts at: 2749 3278 3521 1163
Size: 529 243 4087
Fragments arranged by size:
4087 573 529 393 320 243 91 87
65 31 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2547 2703 2957 3111 3507 4506 4591 5662 2547
Size: 156 254 154 396 999 85 1071 3330
Fragments arranged by size:
3330 1071 999 396 254 156 154 85
BsmI GAATG_Cn'
Cuts at: 3956 4049 3956
Size: 93 6352
BsmBI CGTCTCn'nnnn_
Cuts at: 3686 3686
Size: 6445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2547 2703 2957 3093 3111 3507 3729
Size: 399 757 156 254 136 18 396 222
Cuts at: 3729 3764 4506 4591 5662 1391
Size: 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 757 742 399 396 254 222
156 136 85 35 18
BspEI T'CCGG_A
Cuts at: 2374 6108 2374
Size: 3734 2711
BspHI T'CATG_A
Cuts at: 2352 4334 6064 2352
Size: 1982 1730 2733
Fragments arranged by size:
2733 1982 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 2370 2881 2976 3402 3543 3800 844
Size: 1325 201 511 95 426 141 257 3489
Fragments arranged by size:
3489 1325 511 426 257 201 141 95
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 2821 3603 3742 4332 1394
Size: 429 260 738 782 139 590 3507
Fragments arranged by size:
3507 782 738 590 429 260 139
BsrDI GCAATG_nn'
Cuts at: 65 1248 4940 5114 65
Size: 1183 3692 174 1396
Fragments arranged by size:
3692 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3507 3697 5089 5276 1700
Size: 110 1697 190 1392 187 2869
Fragments arranged by size:
2869 1697 1392 190 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6445
BssSI C'ACGA_G
Cuts at: 4505 5517 4505
Size: 1012 5433
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2668 3267 3517 3686 794
Size: 1874 599 250 169 3553
Fragments arranged by size:
3553 1874 599 250 169
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6445
BstYI r'GATC_y
Cuts at: 1972 2448 3775 4142 4527 4544 5996 6082 6094
Size: 476 1327 367 385 17 1452 86 12
Cuts at: 6094 6440 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1327 476 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2174 2337 2430 2769 513
Size: 552 1109 163 93 339 4189
Fragments arranged by size:
4189 1109 552 339 163 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2227 487
Size: 683 56 1001 4705
Fragments arranged by size:
4705 1001 683 56
BtsI GCAGTG_nn'
Cuts at: 2993 3618 3961 4755 4775 2993
Size: 625 343 794 20 4663
Fragments arranged by size:
4663 794 625 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DraIII CAC_nnn'GTG
Cuts at: 2796 2796
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3077 5452 809
Size: 507 1761 2375 1802
Fragments arranged by size:
2375 1802 1761 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2983 3420
Size: 54 1425 50 275 18 204 949 437
Cuts at: 3420 3892 4781 6410 8
Size: 472 889 1629 43
Fragments arranged by size:
1629 1425 949 889 472 437 275 204
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2294 2477 2537 2684 3464 4375 1353
Size: 786 155 183 60 147 780 911 3423
Fragments arranged by size:
3423 911 786 780 183 155 147 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3320 5032 5406 5552 387
Size: 1064 138 1731 1712 374 146 1280
Fragments arranged by size:
1731 1712 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2545 3109 2545
Size: 564 5881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2120 2201 2246 2315 2408 2518
Size: 393 440 48 81 45 69 93 110
Cuts at: 2518 2519 2534 3584 3605 3725 4508 5105 5892
Size: 1 15 1050 21 120 783 597 787
Cuts at: 5892 6160 1239
Size: 268 1524
Fragments arranged by size:
1524 1050 787 783 597 440 393 268
120 110 93 81 69 48 45 21
15 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2516 3487 2366
Size: 150 971 5324
Fragments arranged by size:
5324 971 150
EcoO109I rG'GnC_Cy
Cuts at: 2023 2047 2467 2902 3580 2023
Size: 24 420 435 678 4888
Fragments arranged by size:
4888 678 435 420 24
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
FspI TGC'GCA
Cuts at: 2672 2687 4951 2672
Size: 15 2264 4166
Fragments arranged by size:
4166 2264 15
HaeII r_GCGC'y
Cuts at: 1678 2529 2593 3044 5592 1678
Size: 851 64 451 2548 2531
Fragments arranged by size:
2548 2531 851 451 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 5086 5118 5160
Size: 32 135 32 647 32 2835 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2835 2626 647 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3232 748
Size: 2484 3961
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KasI G'GCGC_C
Cuts at: 2589 2589
Size: 6445
KpnI G_GTAC'C
Cuts at: 3770 3770
Size: 6445
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5559 5743 1305
Size: 4254 184 2007
Fragments arranged by size:
4254 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 10
Size: 54 1425 343 204 4419
Fragments arranged by size:
4419 1425 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4403 4762 4921 518
Size: 648 3237 359 159 2042
Fragments arranged by size:
3237 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2611 3655 3861 4538 5686 5931 1606
Size: 570 435 1044 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 1044 677 570 435 245 206
NaeI GCC'GGC
Cuts at: 3699 3699
Size: 6445
NarI GG'CG_CC
Cuts at: 2590 2590
Size: 6445
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5893
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6445
NgoMIV G'CCGG_C
Cuts at: 3697 3697
Size: 6445
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 3803 5348 1132
Size: 97 2574 1545 2229
Fragments arranged by size:
2574 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3000 3543 1348
Size: 1652 543 4250
Fragments arranged by size:
4250 1652 543
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6445
PfoI T'CCnGG_A
Cuts at: 2511 2511
Size: 6445
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 6027 6059 4514
Size: 32 1481 32 4900
Fragments arranged by size:
4900 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2023 2047 2902 2023
Size: 24 855 5566
Fragments arranged by size:
5566 855 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PstI C_TGCA'G
Cuts at: 830 2022 2334 2664 2856 2973 3252 3452 3797
Size: 1192 312 330 192 117 279 200 345
Cuts at: 3797 830
Size: 3478
Fragments arranged by size:
3478 1192 345 330 312 279 200 192
117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 3655 3655
Size: 6445
SacI G_AGCT'C
Cuts at: 2547 3111 2547
Size: 564 5881
SacII CC_GC'GG
Cuts at: 2177 2177
Size: 6445
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SapI GCTCTTCn'nnn_
Cuts at: 2294 2684 2294
Size: 390 6055
SbfI CC_TGCA'GG
Cuts at: 2973 3797 2973
Size: 824 5621
ScaI AGT'ACT
Cuts at: 4693 6405 4693
Size: 1712 4733
SexAI A'CCwGG_T
Cuts at: 2792 3116 2792
Size: 324 6121
SfcI C'TryA_G
Cuts at: 826 1045 2018 2330 2660 2852 2969 3248 3448
Size: 219 973 312 330 192 117 279 200
Cuts at: 3448 3591 3793 4928 5609 5800 826
Size: 143 202 1135 681 191 1471
Fragments arranged by size:
1471 1135 973 681 330 312 279 219
202 200 192 191 143 117
SfoI GGC'GCC
Cuts at: 2591 2591
Size: 6445
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 3343 3772 1872
Size: 1471 429 4545
Fragments arranged by size:
4545 1471 429
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 3437 4549 5450 5712 820
Size: 197 926 141 1353 1112 901 262 1553
Fragments arranged by size:
1553 1353 1112 926 901 262 197 141
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 3803 1132
Size: 2671 3774
SrfI GCCC'GGGC
Cuts at: 3343 3343
Size: 6445
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6445
StyI C'CwwG_G
Cuts at: 513 1065 2367 3488 3884 513
Size: 552 1302 1121 396 3074
Fragments arranged by size:
3074 1302 1121 552 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2744 4472 4489 4642 4827 5272 325
Size: 1255 1164 1728 17 153 185 445 1498
Fragments arranged by size:
1728 1498 1255 1164 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 2537 3282
Size: 274 80 33 51 1443 528 32 745
Cuts at: 3282 4691 6403 96
Size: 1409 1712 138
Fragments arranged by size:
1712 1443 1409 745 528 274 138 80
51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3469 3501 3597 3629 6405 6437 3469
Size: 32 96 32 2776 32 3477
Fragments arranged by size:
3477 2776 96 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6445
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2941 2111
Size: 830 5615
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 3341 3770 1870
Size: 1471 429 4545
Fragments arranged by size:
4545 1471 429
XmnI GAAnn'nnTTC
Cuts at: 2289 4574 2289
Size: 2285 4160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI
SwaI