Restriction map for
pFN21AA1181
(Circular) (Six-base) MAPSORT of: pFN21AA1181.seq Check: 1418 from: 1 to: 5575
pFN21AA1181 5575 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2217 3385 278
Size: 53 83 186 936 681 1168 2468
Fragments arranged by size:
2468 1168 936 681 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2591 3638 5022 5290 1632
Size: 370 589 1047 1384 268 1917
Fragments arranged by size:
1917 1384 1047 589 370 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 2397 4474 5451 1203
Size: 1194 2077 977 1327
Fragments arranged by size:
2077 1327 1194 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2749 2837 3318 4890 2749
Size: 88 481 1572 3434
Fragments arranged by size:
3434 1572 481 88
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2884 3110 3262 5371 1493
Size: 1391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2516 2900 1870
Size: 73 573 384 4545
Fragments arranged by size:
4545 573 384 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2896 4351 618
Size: 325 217 627 257 852 1455 1842
Fragments arranged by size:
1842 1455 852 627 325 257 217
BanII G_rGCy'C
Cuts at: 1391 2136 2172 2894 1391
Size: 745 36 722 4072
Fragments arranged by size:
4072 745 722 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BbvCI CC'TCA_GC
Cuts at: 2708 2708
Size: 5575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3766 3800 1903
Size: 34 50 34 1745 34 3678
Fragments arranged by size:
3678 1745 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BfrBI ATG'CAT
Cuts at: 2823 2872 2823
Size: 49 5526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2670 2840 4186
Size: 107 122 71 631 605 998 170 1346
Cuts at: 4186 136
Size: 1525
Fragments arranged by size:
1525 1346 998 631 605 170 122 107
71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2583 3636 4792 1790
Size: 259 534 1053 1156 2573
Fragments arranged by size:
2573 1156 1053 534 259
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3306 4264 442
Size: 416 53 83 399 24 1889 958 1753
Fragments arranged by size:
1889 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4235 1239
Size: 2996 2579
Bpu10I CC'TnA_GC
Cuts at: 2708 2708
Size: 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2398 3366 493
Size: 711 1194 968 2702
Fragments arranged by size:
2702 1194 968 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 3382 3764 4399
Size: 53 83 186 936 681 1168 382 635
Cuts at: 4399 275
Size: 1451
Fragments arranged by size:
1451 1168 936 681 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2138 4008 4406 4680 4827 5238 2138
Size: 1870 398 274 147 411 2475
Fragments arranged by size:
2475 1870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2447 2477 1178
Size: 30 165 30 1044 30 4276
Fragments arranged by size:
4276 1044 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5575
BseYI C'CCAG_C
Cuts at: 2421 2860 4778 5274 2421
Size: 439 1918 496 2722
Fragments arranged by size:
2722 1918 496 439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 1163
Size: 573 492 4510
Fragments arranged by size:
4510 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 2172 3636 3721 4792 2136
Size: 36 1464 85 1071 2919
Fragments arranged by size:
2919 1464 1071 85 36
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
BsmBI CGTCTCn'nnnn_
Cuts at: 2731 2731
Size: 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2136 2172 2583 2894 3636 3721
Size: 399 259 87 36 411 311 742 85
Cuts at: 3721 4792 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 311 259 87
85 36
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2804 4219 4406 1700
Size: 110 994 1415 187 2869
Fragments arranged by size:
2869 1415 994 187 110
BsrGI T'GTAC_A
Cuts at: 96 2751 96
Size: 2655 2920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstXI CCAn_nnnn'nTGG
Cuts at: 2548 2548
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2322 2853 2905 3272 3657 3674 5126 5212
Size: 350 531 52 367 385 17 1452 86
Cuts at: 5212 5224 5570 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 531 385 367 350 346 86
52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2497 2583 513
Size: 552 1432 86 3505
Fragments arranged by size:
3505 1432 552 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3091 3885 3905 2245
Size: 846 794 20 3915
Fragments arranged by size:
3915 846 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DraIII CAC_nnn'GTG
Cuts at: 2296 2411 2296
Size: 115 5460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2637 3022 3911
Size: 54 1425 50 275 18 807 385 889
Cuts at: 3911 5540 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 807 385 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2120 2126 3505 1353
Size: 758 9 6 1379 3423
Fragments arranged by size:
3423 1379 758 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2134 2170 2134
Size: 36 5539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2591 3638 4235 5022 5290 1239
Size: 393 370 589 1047 597 787 268 1524
Fragments arranged by size:
1524 1047 787 597 589 393 370 268
EcoO109I rG'GnC_Cy
Cuts at: 2053 2380 2790 2053
Size: 327 410 4838
Fragments arranged by size:
4838 410 327
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 5575
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4216 4248 4290 4322 1373
Size: 32 135 32 2644 32 42 32 2626
Fragments arranged by size:
2644 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 4689 4873 1305
Size: 1260 2124 184 2007
Fragments arranged by size:
2124 2007 1260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2639 10
Size: 54 1425 343 807 2946
Fragments arranged by size:
2946 1425 807 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 3892 4051 518
Size: 648 2367 359 159 2042
Fragments arranged by size:
2367 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2199 2329 2614 2749 2860 2991 3668 4816
Size: 593 130 285 135 111 131 677 1148
Cuts at: 4816 5061 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 593 285 245 135 131
130 111
NaeI GCC'GGC
Cuts at: 2806 2806
Size: 5575
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5023
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NgoMIV G'CCGG_C
Cuts at: 2804 2804
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NsiI A_TGCA'T
Cuts at: 2825 2874 2825
Size: 49 5526
NspI r_CATG'y
Cuts at: 1132 1229 2450 2876 2933 4478 1132
Size: 97 1221 426 57 1545 2229
Fragments arranged by size:
2229 1545 1221 426 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PfoI T'CCnGG_A
Cuts at: 2811 2811
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PmlI CAC'GTG
Cuts at: 2398 2398
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2380 3644 3676 5157 5189 2348
Size: 32 1264 32 1481 32 2734
Fragments arranged by size:
2734 1481 1264 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 2380 2790 2053
Size: 327 410 4838
Fragments arranged by size:
4838 410 327
PshAI GACnn'nnGTC
Cuts at: 1613 2217 2447 1613
Size: 604 230 4741
Fragments arranged by size:
4741 604 230
PsiI TTA'TAA
Cuts at: 2147 3145 2147
Size: 998 4577
PstI C_TGCA'G
Cuts at: 830 2927 830
Size: 2097 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
PvuII CAG'CTG
Cuts at: 2860 2860
Size: 5575
SacI G_AGCT'C
Cuts at: 2136 2172 2136
Size: 36 5539
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2035 2480 2659 2923 4058 4739 4930
Size: 219 990 445 179 264 1135 681 191
Cuts at: 4930 826
Size: 1471
Fragments arranged by size:
1471 1135 990 681 445 264 219 191
179
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 2843 1517
Size: 1326 4249
StyI C'CwwG_G
Cuts at: 513 1065 2049 3014 513
Size: 552 984 965 3074
Fragments arranged by size:
3074 984 965 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3602 3619 3772 3957 4402 325
Size: 1255 2022 17 153 185 445 1498
Fragments arranged by size:
2022 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2628 2727 2751
Size: 274 80 33 51 1443 651 99 24
Cuts at: 2751 3821 5533 96
Size: 1070 1712 138
Fragments arranged by size:
1712 1443 1070 651 274 138 99 80
51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 5535 5567 2447
Size: 32 3056 32 2455
Fragments arranged by size:
3056 2455 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2215 3383 276
Size: 53 83 186 936 681 1168 2468
Fragments arranged by size:
2468 1168 936 681 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NarI NruI PacI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI