Restriction map for
pFN21AA1170
(Circular) (Six-base) MAPSORT of: pFN21AA1170.seq Check: 8244 from: 1 to: 6979
pFN21AA1170 6979 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4789 278
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4322 1937
Size: 2385 4594
Acc65I G'GTAC_C
Cuts at: 3493 4300 3493
Size: 807 6172
AclI AA'CG_TT
Cuts at: 1563 5106 5479 1563
Size: 3543 373 3063
Fragments arranged by size:
3543 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2937 3854 5042 6426 6694 1632
Size: 767 538 917 1188 1384 268 1917
Fragments arranged by size:
1917 1384 1188 917 767 538 268
AflII C'TTAA_G
Cuts at: 820 1017 3566 820
Size: 197 2549 4233
Fragments arranged by size:
4233 2549 197
AflIII A'CryG_T
Cuts at: 1203 5878 6855 1203
Size: 4675 977 1327
Fragments arranged by size:
4675 1327 977
AgeI A'CCGG_T
Cuts at: 5810 5810
Size: 6979
AhdI GACnn_n'nnGTC
Cuts at: 5708 5708
Size: 6979
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6979
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 6947
AlwNI CAG_nnn'CTG
Cuts at: 4722 6294 4722
Size: 1572 5407
ApaI G_GGCC'C
Cuts at: 2039 3268 4096 2039
Size: 1229 828 4922
Fragments arranged by size:
4922 1229 828
ApaLI G'TGCA_C
Cuts at: 5036 6192 5036
Size: 1156 5823
ApoI r'AATT_y
Cuts at: 1493 2231 4288 4514 4666 6775 1493
Size: 738 2057 226 152 2109 1697
Fragments arranged by size:
2109 2057 1697 738 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5533 160
Size: 872 4501 1606
Fragments arranged by size:
4501 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
AvaI C'yCGr_G
Cuts at: 1870 1943 2388 2685 4304 1870
Size: 73 445 297 1619 4545
Fragments arranged by size:
4545 1619 445 297 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3345 3378 3345
Size: 33 6946
BamHI G'GATC_C
Cuts at: 4309 4676 4309
Size: 367 6612
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2998 3196 3217 3465
Size: 325 217 627 299 912 198 21 248
Cuts at: 3465 3493 4075 4167 4300 5755 618
Size: 28 582 92 133 1455 1842
Fragments arranged by size:
1842 1455 912 627 582 325 299 248
217 198 133 92 28 21
BanII G_rGCy'C
Cuts at: 1391 2039 2138 2601 3111 3268 4096 4298 1391
Size: 648 99 463 510 157 828 202 4072
Fragments arranged by size:
4072 828 648 510 463 202 157 99
BbeI G_GCGC'C
Cuts at: 2090 4171 2090
Size: 2081 4898
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2925 3401 4257 928
Size: 971 1026 476 856 3650
Fragments arranged by size:
3650 1026 971 856 476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5170 5204 1903
Size: 34 3233 34 3678
Fragments arranged by size:
3678 3233 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2936 4871 6081 2936
Size: 1935 1210 3834
Fragments arranged by size:
3834 1935 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6979
BfrBI ATG'CAT
Cuts at: 2722 2722
Size: 6979
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 3030 5590
Size: 107 122 71 631 605 649 709 2560
Cuts at: 5590 136
Size: 1525
Fragments arranged by size:
2560 1525 709 649 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6974 6974
Size: 6979
BlpI GC'TnA_GC
Cuts at: 3231 4396 3231
Size: 1165 5814
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2560 3268 3470 4096 5040 6196 1790
Size: 249 521 708 202 626 944 1156 2573
Fragments arranged by size:
2573 1156 944 708 626 521 249 202
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6979
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4252 4710 5668
Size: 416 53 83 399 24 2835 458 958
Cuts at: 5668 442
Size: 1753
Fragments arranged by size:
2835 1753 958 458 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2318 2531 2669 3479 3650 3975 3997
Size: 938 141 213 138 810 171 325 22
Cuts at: 3997 5639 1239
Size: 1642 2579
Fragments arranged by size:
2579 1642 938 810 325 213 171 141
138 22
Bpu10I CC'TnA_GC
Cuts at: 2600 2600
Size: 6979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2259 4082 5104 5969 6267 2259
Size: 1823 1022 865 298 2971
Fragments arranged by size:
2971 1823 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2684 5642 882
Size: 617 1185 2958 2219
Fragments arranged by size:
2958 2219 1185 617
BsaAI yAC'GTr
Cuts at: 493 1204 4770 493
Size: 711 3566 2702
Fragments arranged by size:
3566 2702 711
BsaBI GATnn'nnATC
Cuts at: 3653 6973 3653
Size: 3320 3659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 3503 4168 4786
Size: 53 83 186 936 554 1416 665 618
Cuts at: 4786 5168 5803 275
Size: 382 635 1451
Fragments arranged by size:
1451 1416 936 665 635 618 554 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5412 5810 6084 6231 6642 5412
Size: 398 274 147 411 5749
Fragments arranged by size:
5749 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 1178
Size: 30 165 30 747 30 5977
Fragments arranged by size:
5977 747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2741 2744 2778 3038 3119 3134 3143 3467
Size: 1555 3 34 260 81 15 9 324
Cuts at: 3467 3659 3737 1186
Size: 192 78 4428
Fragments arranged by size:
4428 1555 324 260 192 81 78 34
15 9 3
BseYI C'CCAG_C
Cuts at: 3679 6182 6678 3679
Size: 2503 496 3980
Fragments arranged by size:
3980 2503 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 3455 1163
Size: 573 564 1155 4687
Fragments arranged by size:
4687 1155 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4429 5190 6218 1540
Size: 275 186 2428 761 1028 2301
Fragments arranged by size:
2428 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 3297 5040 5125 6196 2601
Size: 696 1743 85 1071 3384
Fragments arranged by size:
3384 1743 1071 696 85
BsmI GAATG_Cn'
Cuts at: 3534 4490 4583 3534
Size: 956 93 5930
Fragments arranged by size:
5930 956 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3299 3494 3299
Size: 195 6784
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2138 2560 2601 3111 3268 3297
Size: 399 249 99 422 41 510 157 29
Cuts at: 3297 3470 4096 4298 5040 5125 6196 1391
Size: 173 626 202 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 626 510 422 399 249
202 173 157 99 85 41 29
BspEI T'CCGG_A
Cuts at: 6642 6642
Size: 6979
BspHI T'CATG_A
Cuts at: 4868 6598 4868
Size: 1730 5249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3183 4334 844
Size: 2339 1151 3489
Fragments arranged by size:
3489 2339 1151
BsrBI CCG'CTC
Cuts at: 1394 1823 3235 4866 1394
Size: 429 1412 1631 3507
Fragments arranged by size:
3507 1631 1412 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2059 5474 5648 65
Size: 1183 811 3415 174 1396
Fragments arranged by size:
3415 1396 1183 811 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2346 5623 5810 1700
Size: 110 536 3277 187 2869
Fragments arranged by size:
3277 2869 536 187 110
BsrGI T'GTAC_A
Cuts at: 96 3534 96
Size: 3438 3541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6979
BssSI C'ACGA_G
Cuts at: 5039 6051 5039
Size: 1012 5967
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6979
BstBI TT'CG_AA
Cuts at: 5783 5783
Size: 6979
BstEII G'GTnAC_C
Cuts at: 1184 2761 1184
Size: 1577 5402
BstXI CCAn_nnnn'nTGG
Cuts at: 2509 4057 4162 2509
Size: 1548 105 5326
Fragments arranged by size:
5326 1548 105
BstYI r'GATC_y
Cuts at: 1972 3088 4309 4676 5061 5078 6530 6616 6628
Size: 1116 1221 367 385 17 1452 86 12
Cuts at: 6628 6974 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1221 1116 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3642 4007 3642
Size: 365 6614
BtgI C'CryG_G
Cuts at: 513 1065 2004 2757 3028 513
Size: 552 939 753 271 4464
Fragments arranged by size:
4464 939 753 552 271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3409 487
Size: 683 56 2183 4057
Fragments arranged by size:
4057 2183 683 56
BtsI GCAGTG_nn'
Cuts at: 2560 2782 3867 4495 5289 5309 2560
Size: 222 1085 628 794 20 4230
Fragments arranged by size:
4230 1085 794 628 222 20
ClaI AT'CG_AT
Cuts at: 1554 3687 1554
Size: 2133 4846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6944
DraI TTT'AAA
Cuts at: 4069 4283 4069
Size: 214 6765
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5986 809
Size: 507 4670 1802
Fragments arranged by size:
4670 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4426 5315 6944
Size: 54 1425 50 275 18 2596 889 1629
Cuts at: 6944 8
Size: 43
Fragments arranged by size:
2596 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4426 1537
Size: 275 2614 4090
Fragments arranged by size:
4090 2614 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2933 3098 3120 3444 3480 4909 1353
Size: 1580 165 22 324 36 1429 3423
Fragments arranged by size:
3423 1580 1429 324 165 36 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5566 5940 6086 387
Size: 1064 138 3977 374 146 1280
Fragments arranged by size:
3977 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2599 2599
Size: 6979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2318 2399 2531 2669 2937 3479
Size: 393 545 141 81 132 138 268 542
Cuts at: 3479 3650 3854 3975 3997 5042 5639 6426 6694
Size: 171 204 121 22 1045 597 787 268
Cuts at: 6694 1239
Size: 1524
Fragments arranged by size:
1524 1045 787 597 545 542 393 268
268 204 171 141 138 132 121 81
22
EcoNI CCTnn'n_nnAGG
Cuts at: 3341 3521 3641 3341
Size: 180 120 6679
Fragments arranged by size:
6679 180 120
EcoO109I rG'GnC_Cy
Cuts at: 2011 2035 2493 2809 3187 3253 3265 4093 2011
Size: 24 458 316 378 66 12 828 4897
Fragments arranged by size:
4897 828 458 378 316 66 24 12
EcoRI G'AATT_C
Cuts at: 2231 4288 4666 2231
Size: 2057 378 4544
Fragments arranged by size:
4544 2057 378
FspI TGC'GCA
Cuts at: 5485 5485
Size: 6979
HaeII r_GCGC'y
Cuts at: 1678 2090 4171 6126 1678
Size: 412 2081 1955 2531
Fragments arranged by size:
2531 2081 1955 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5620 5652 5694 5726 1373
Size: 32 135 32 4048 32 42 32 2626
Fragments arranged by size:
4048 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4323 4569 669
Size: 988 281 2385 246 3079
Fragments arranged by size:
3079 2385 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2710 748
Size: 1962 5017
HpaI GTT'AAC
Cuts at: 4569 4569
Size: 6979
KasI G'GCGC_C
Cuts at: 2086 4167 2086
Size: 2081 4898
KpnI G_GTAC'C
Cuts at: 3497 4304 3497
Size: 807 6172
MfeI C'AATT_G
Cuts at: 4578 4578
Size: 6979
MluI A'CGCG_T
Cuts at: 6855 6855
Size: 6979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2283 3343 6093 6277 1305
Size: 978 1060 2750 184 2007
Fragments arranged by size:
2750 2007 1060 978 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5157
Fragments arranged by size:
5157 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3387 3689 4937 5296 5455 518
Size: 648 2221 302 1248 359 159 2042
Fragments arranged by size:
2221 2042 1248 648 359 302 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2573 2995 4395 5072 6220 6465 1606
Size: 513 454 422 1400 677 1148 245 2120
Fragments arranged by size:
2120 1400 1148 677 513 454 422 245
NarI GG'CG_CC
Cuts at: 2087 4168 2087
Size: 2081 4898
NcoI C'CATG_G
Cuts at: 513 1065 2004 2757 513
Size: 552 939 753 4735
Fragments arranged by size:
4735 939 753 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6979
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6979
NotI GC'GGCC_GC
Cuts at: 4426 4426
Size: 6979
NsiI A_TGCA'T
Cuts at: 2724 2724
Size: 6979
NspI r_CATG'y
Cuts at: 1132 1229 2189 2947 4337 5882 1132
Size: 97 960 758 1390 1545 2229
Fragments arranged by size:
2229 1545 1390 960 758 97
PasI CC'CwG_GG
Cuts at: 1348 3261 3337 1348
Size: 1913 76 4990
Fragments arranged by size:
4990 1913 76
PciI A'CATG_T
Cuts at: 5878 5878
Size: 6979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6979
PfoI T'CCnGG_A
Cuts at: 2508 3891 3915 4043 2508
Size: 1383 24 128 5444
Fragments arranged by size:
5444 1383 128 24
PmeI GTTT'AAAC
Cuts at: 4283 4283
Size: 6979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5048 5080 6561 6593 5048
Size: 32 1481 32 5434
Fragments arranged by size:
5434 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2493 2809 3187 2011
Size: 482 316 378 5803
Fragments arranged by size:
5803 482 378 316
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6979
PsiI TTA'TAA
Cuts at: 4549 4549
Size: 6979
PspOMI G'GGCC_C
Cuts at: 2035 3264 4092 2035
Size: 1229 828 4922
Fragments arranged by size:
4922 1229 828
PstI C_TGCA'G
Cuts at: 830 2777 2868 3586 4331 830
Size: 1947 91 718 745 3478
Fragments arranged by size:
3478 1947 745 718 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6979
PvuII CAG'CTG
Cuts at: 2573 2995 2573
Size: 422 6557
SacI G_AGCT'C
Cuts at: 2601 2601
Size: 6979
SalI G'TCGA_C
Cuts at: 1936 4321 1936
Size: 2385 4594
SapI GCTCTTCn'nnn_
Cuts at: 2933 2933
Size: 6979
SbfI CC_TGCA'GG
Cuts at: 4331 4331
Size: 6979
ScaI AGT'ACT
Cuts at: 5227 6939 5227
Size: 1712 5267
SfcI C'TryA_G
Cuts at: 826 1045 2548 2773 2864 3582 4327 5462 6143
Size: 219 1503 225 91 718 745 1135 681
Cuts at: 6143 6334 826
Size: 191 1471
Fragments arranged by size:
1503 1471 1135 745 718 681 225 219
191 91
SfoI GGC'GCC
Cuts at: 2088 4169 2088
Size: 2081 4898
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6979
SmaI CCC'GGG
Cuts at: 1872 2390 2687 4306 1872
Size: 518 297 1619 4545
Fragments arranged by size:
4545 1619 518 297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2274 3566 4097 5083 5984 6246
Size: 197 926 331 1292 531 986 901 262
Cuts at: 6246 820
Size: 1553
Fragments arranged by size:
1553 1292 986 926 901 531 331 262
197
SnaBI TAC'GTA
Cuts at: 493 4770 493
Size: 4277 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6979
SphI G_CATG'C
Cuts at: 1132 2189 2947 4337 1132
Size: 1057 758 1390 3774
Fragments arranged by size:
3774 1390 1057 758
SrfI GCCC'GGGC
Cuts at: 2390 2390
Size: 6979
SspI AAT'ATT
Cuts at: 5 52 2641 4903 5
Size: 47 2589 2262 2081
Fragments arranged by size:
2589 2262 2081 47
StuI AGG'CCT
Cuts at: 1517 2324 2546 1517
Size: 807 222 5950
Fragments arranged by size:
5950 807 222
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 2410 2757 3156 3249 4418
Size: 552 939 87 319 347 399 93 1169
Cuts at: 4418 513
Size: 3074
Fragments arranged by size:
3074 1169 939 552 399 347 319 93
87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2661 5006 5023 5176 5361 5806 325
Size: 1255 1081 2345 17 153 185 445 1498
Fragments arranged by size:
2345 1498 1255 1081 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2878 3534 5225
Size: 274 80 33 51 1443 901 656 1691
Cuts at: 5225 6937 96
Size: 1712 138
Fragments arranged by size:
1712 1691 1443 901 656 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6939 6971 6939
Size: 32 6947
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6979
XbaI T'CTAG_A
Cuts at: 4315 4315
Size: 6979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2417 2417
Size: 6979
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6979
XmaI C'CCGG_G
Cuts at: 1870 2388 2685 4304 1870
Size: 518 297 1619 4545
Fragments arranged by size:
4545 1619 518 297
XmnI GAAnn'nnTTC
Cuts at: 2640 3320 3839 5108 2640
Size: 680 519 1269 4511
Fragments arranged by size:
4511 1269 680 519
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4787 276
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbvCI BplI BsiWI BstZ17I
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SwaI