Restriction map for
pFN21AA1162
(Circular) (Six-base) MAPSORT of: pFN21AA1162.seq Check: 7692 from: 1 to: 5752
pFN21AA1162 5752 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2976 3815 5199 5467 1632
Size: 608 736 839 1384 268 1917
Fragments arranged by size:
1917 1384 839 736 608 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 2996 1166
Size: 1830 3922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaI G_GGCC'C
Cuts at: 2907 2907
Size: 5752
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2177 2550 3061 3287 3439 5548 1493
Size: 684 373 511 226 152 2109 1697
Fragments arranged by size:
2109 1697 684 511 373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2928 3077 1870
Size: 73 141 844 149 4545
Fragments arranged by size:
4545 844 149 141 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2907 3071 1391
Size: 1516 164 4072
Fragments arranged by size:
4072 1516 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2397 3012 928
Size: 971 498 615 3668
Fragments arranged by size:
3668 971 615 498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 2896 1548
Size: 1348 4404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4363 136
Size: 107 122 71 631 605 2691 1525
Fragments arranged by size:
2691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2809 5747 2809
Size: 2938 2814
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2907 3813 4969 1790
Size: 1117 906 1156 2573
Fragments arranged by size:
2573 1156 1117 906
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4412 1239
Size: 3173 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3034 3877 4742 5040 3034
Size: 843 865 298 3746
Fragments arranged by size:
3746 865 843 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2150 2180 1178
Size: 30 165 30 747 30 4750
Fragments arranged by size:
4750 747 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2717 2753 2858 2861 2891 2963 1186
Size: 916 615 36 105 3 30 72 3975
Fragments arranged by size:
3975 916 615 105 72 36 30 3
BseYI C'CCAG_C
Cuts at: 2329 2983 4955 5451 2329
Size: 654 1972 496 2630
Fragments arranged by size:
2630 1972 654 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2907 3071 3813 3898 4969 1391
Size: 399 1117 164 742 85 1071 2174
Fragments arranged by size:
2174 1117 1071 742 399 164 85
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 3107 844
Size: 1559 704 3489
Fragments arranged by size:
3489 1559 704
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 2388 4556 2388
Size: 2168 3584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2809 3082 3449 3834 3851 5303 5389 5401
Size: 837 273 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 837 385 367 346 273 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2946 2946
Size: 5752
BtgI C'CryG_G
Cuts at: 513 1065 2004 2189 513
Size: 552 939 185 4076
Fragments arranged by size:
4076 939 552 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2832 487
Size: 683 56 1606 3407
Fragments arranged by size:
3407 1606 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2305 3199
Size: 54 1425 50 275 18 216 259 894
Cuts at: 3199 4088 5717 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 894 889 275 259 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 2835 3682 1353
Size: 1440 42 847 3423
Fragments arranged by size:
3423 1440 847 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2351 4339 4713 4859 387
Size: 1064 138 762 1988 374 146 1280
Fragments arranged by size:
1988 1280 1064 762 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2976 3815 4412 5199 5467 1239
Size: 393 608 736 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 736 608 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2081 2903 2904 2081
Size: 822 1 4929
Fragments arranged by size:
4929 822 1
EcoRI G'AATT_C
Cuts at: 2550 3061 3439 2550
Size: 511 378 4863
Fragments arranged by size:
4863 511 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2798 2828 2798
Size: 30 5722
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2020 2042 4899 1678
Size: 342 22 2857 2531
Fragments arranged by size:
2857 2531 342 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2503 2535 4393 4425 4467
Size: 32 135 32 931 32 1858 32 42
Cuts at: 4467 4499 1373
Size: 32 2626
Fragments arranged by size:
2626 1858 931 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 4866 5050 1305
Size: 858 2703 184 2007
Fragments arranged by size:
2703 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2996 3710 4069 4228 518
Size: 648 1830 714 359 159 2042
Fragments arranged by size:
2042 1830 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2987 3168 3845 4993 5238 1606
Size: 1381 181 677 1148 245 2120
Fragments arranged by size:
2120 1381 1148 677 245 181
NcoI C'CATG_G
Cuts at: 513 1065 2004 2189 513
Size: 552 939 185 4076
Fragments arranged by size:
4076 939 552 185
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3038 3110 4655 1132
Size: 97 1809 72 1545 2229
Fragments arranged by size:
2229 1809 1545 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspOMI G'GGCC_C
Cuts at: 2903 2903
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2400 3104 830
Size: 1570 704 3478
Fragments arranged by size:
3478 1570 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2987 2987
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SexAI A'CCwGG_T
Cuts at: 2293 2293
Size: 5752
SfcI C'TryA_G
Cuts at: 826 1045 2396 3100 4235 4916 5107 826
Size: 219 1351 704 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 704 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2930 3079 1872
Size: 1058 149 4545
Fragments arranged by size:
4545 1058 149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3013 3856 4757 5019 820
Size: 197 926 1070 843 901 262 1553
Fragments arranged by size:
1553 1070 926 901 843 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3038 3110 1132
Size: 1906 72 3774
Fragments arranged by size:
3774 1906 72
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 2189 2592 2660 3191 513
Size: 552 939 185 403 68 531 3074
Fragments arranged by size:
3074 939 552 531 403 185 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2150 2182 5712 5744 2150
Size: 32 3530 32 2158
Fragments arranged by size:
3530 2158 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2146
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2928 3077 1870
Size: 1058 149 4545
Fragments arranged by size:
4545 1058 149
XmnI GAAnn'nnTTC
Cuts at: 2253 3881 2253
Size: 1628 4124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI