Restriction map for
pFN21AA1160
(Circular) (Six-base) MAPSORT of: pFN21AA1160.seq Check: 3823 from: 1 to: 5698
pFN21AA1160 5698 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2444 4597 5574 1203
Size: 1241 2153 977 1327
Fragments arranged by size:
2153 1327 1241 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 2204 2211 3007 3233 3385 5494 1493
Size: 711 7 796 226 152 2109 1697
Fragments arranged by size:
2109 1697 796 711 226 152 7
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2976 3019 4474 618
Size: 325 217 627 1189 43 1455 1842
Fragments arranged by size:
1842 1455 1189 627 325 217 43
BanII G_rGCy'C
Cuts at: 1391 2457 2965 3017 1391
Size: 1066 508 52 4072
Fragments arranged by size:
4072 1066 508 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 928
Size: 971 196 4531
Fragments arranged by size:
4531 971 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 2929 4309
Size: 107 122 71 631 605 361 896 1380
Cuts at: 4309 136
Size: 1525
Fragments arranged by size:
1525 1380 896 631 605 361 122 107
71
BglII A'GATC_T
Cuts at: 2160 2861 5693 2160
Size: 701 2832 2165
Fragments arranged by size:
2832 2165 701
BlpI GC'TnA_GC
Cuts at: 2058 2451 3115 2058
Size: 393 664 4641
Fragments arranged by size:
4641 664 393
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 2731 1318
Size: 1413 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2272 3429 4387
Size: 416 53 83 399 24 855 1157 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1157 958 855 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2604 2604
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2780 4358 1239
Size: 1541 1578 2579
Fragments arranged by size:
2579 1578 1541
Bpu10I CC'TnA_GC
Cuts at: 2038 2246 2038
Size: 208 5490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 4361 882
Size: 617 1308 1554 2219
Fragments arranged by size:
2219 1554 1308 617
BsaAI yAC'GTr
Cuts at: 493 1204 2447 3489 493
Size: 711 1243 1042 2702
Fragments arranged by size:
2702 1243 1042 711
BsaBI GATnn'nnATC
Cuts at: 2642 5692 2642
Size: 3050 2648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2271 4131 4529 4803 4950 5361 2271
Size: 1860 398 274 147 411 2608
Fragments arranged by size:
2608 1860 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5473
Fragments arranged by size:
5473 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2421 2616 2630 2633 2642 2675 2678 2774
Size: 1235 195 14 3 9 33 3 96
Cuts at: 2774 2783 2973 1186
Size: 9 190 3911
Fragments arranged by size:
3911 1235 195 190 96 33 14 9
9 3 3
BseYI C'CCAG_C
Cuts at: 2284 4901 5397 2284
Size: 2617 496 2585
Fragments arranged by size:
2617 2585 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2876 3148 3909 4937 1540
Size: 275 186 875 272 761 1028 2301
Fragments arranged by size:
2301 1028 875 761 275 272 186
BsiHKAI G_wGCw'C
Cuts at: 2457 2965 3759 3844 4915 2457
Size: 508 794 85 1071 3240
Fragments arranged by size:
3240 1071 794 508 85
BsmI GAATG_Cn'
Cuts at: 2194 3209 3302 2194
Size: 1015 93 4590
Fragments arranged by size:
4590 1015 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2134 2134
Size: 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2457 2965 3017 3759 3844 4915 1391
Size: 399 667 508 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 667 508 399 85 52
BspEI T'CCGG_A
Cuts at: 2271 5361 2271
Size: 3090 2608
BspHI T'CATG_A
Cuts at: 2329 2457 3587 5317 2329
Size: 128 1130 1730 2710
Fragments arranged by size:
2710 1730 1130 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2886 3585 1394
Size: 429 1063 699 3507
Fragments arranged by size:
3507 1063 699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 4193 4367 65
Size: 1183 1473 1472 174 1396
Fragments arranged by size:
1473 1472 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2875 4342 4529 1700
Size: 110 1065 1467 187 2869
Fragments arranged by size:
2869 1467 1065 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 2209 4502 2209
Size: 2293 3405
BstEII G'GTnAC_C
Cuts at: 1184 2486 1184
Size: 1302 4396
BstYI r'GATC_y
Cuts at: 1972 2160 2861 3028 3395 3780 3797 5249 5335
Size: 188 701 167 367 385 17 1452 86
Cuts at: 5335 5347 5693 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 701 385 367 346 188 167
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2925 3214 4008 4028 2925
Size: 289 794 20 4595
Fragments arranged by size:
4595 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 2469 1554
Size: 915 4783
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2873 2877 3145
Size: 54 1425 50 275 18 1043 4 268
Cuts at: 3145 4034 5663 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1043 889 275 268 54 50
43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2873 3145 1537
Size: 275 1061 272 4090
Fragments arranged by size:
4090 1061 275 272
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 2430 2607 2619 2628 3628 1353
Size: 708 369 177 12 9 1000 3423
Fragments arranged by size:
3423 1000 708 369 177 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2455 2963 2455
Size: 508 5190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2780 3761 4358 5145 5413 1239
Size: 393 1148 981 597 787 268 1524
Fragments arranged by size:
1524 1148 981 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2291 2291
Size: 5698
EcoRI G'AATT_C
Cuts at: 2211 3007 3385 2211
Size: 796 378 4524
Fragments arranged by size:
4524 796 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2441 2441
Size: 5698
FseI GG_CCGG'CC
Cuts at: 2879 2879
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2133 2165 2651 2683 4339
Size: 32 135 32 561 32 486 32 1656
Cuts at: 4339 4371 4413 4445 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1656 561 486 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2649 4812 4996 1305
Size: 1344 2163 184 2007
Fragments arranged by size:
2163 2007 1344 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 3114 3791 4939 5184 1606
Size: 456 1052 677 1148 245 2120
Fragments arranged by size:
2120 1148 1052 677 456 245
NaeI GCC'GGC
Cuts at: 2877 2877
Size: 5698
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NgoMIV G'CCGG_C
Cuts at: 2875 2875
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PmlI CAC'GTG
Cuts at: 2447 2447
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2291 2291
Size: 5698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2823 2861 3050 830
Size: 1993 38 189 3478
Fragments arranged by size:
3478 1993 189 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2062 2062
Size: 5698
SacI G_AGCT'C
Cuts at: 2457 2965 2457
Size: 508 5190
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 2823 3050 2823
Size: 227 5471
ScaI AGT'ACT
Cuts at: 2369 2480 3946 5658 2369
Size: 111 1466 1712 2409
Fragments arranged by size:
2409 1712 1466 111
SfcI C'TryA_G
Cuts at: 826 1045 2819 2857 3046 4181 4862 5053 826
Size: 219 1774 38 189 1135 681 191 1471
Fragments arranged by size:
1774 1471 1135 681 219 191 189 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 2033 2033
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 3137 513
Size: 552 939 1133 3074
Fragments arranged by size:
3074 1133 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2917 3725 3742 3895 4080 4525 325
Size: 1255 1337 808 17 153 185 445 1498
Fragments arranged by size:
1498 1337 1255 808 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2111 2367 2478
Size: 274 80 33 51 1443 134 256 111
Cuts at: 2478 3944 5656 96
Size: 1466 1712 138
Fragments arranged by size:
1712 1466 1443 274 256 138 134 111
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2848 2848
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 2424 3827 2424
Size: 1403 4295
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FspAI KasI NarI NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfoI SrfI SwaI