Restriction map for
pFN21AA1154
(Circular) (Six-base) MAPSORT of: pFN21AA1154.seq Check: 3048 from: 1 to: 6133
pFN21AA1154 6133 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3045 3339 3476 1937
Size: 1108 294 137 4594
Fragments arranged by size:
4594 1108 294 137
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 2393 2846 4196 5580 5848 1632
Size: 536 225 453 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 536 453 268 225
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 2382 5032 6009 1203
Size: 1179 2650 977 1327
Fragments arranged by size:
2650 1327 1179 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 2824 3876 5448 2824
Size: 1052 1572 3509
Fragments arranged by size:
3509 1572 1052
ApaLI G'TGCA_C
Cuts at: 2419 4190 5346 2419
Size: 1771 1156 3206
Fragments arranged by size:
3206 1771 1156
ApoI r'AATT_y
Cuts at: 1493 2854 2899 3442 3668 3820 5929 1493
Size: 1361 45 543 226 152 2109 1697
Fragments arranged by size:
2109 1697 1361 543 226 152 45
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2991 3024 2991
Size: 33 6100
BamHI G'GATC_C
Cuts at: 2741 2795 3463 3830 2741
Size: 54 668 367 5044
Fragments arranged by size:
5044 668 367 54
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2097 2173 3224 3454 4909
Size: 325 217 627 310 76 1051 230 1455
Cuts at: 4909 618
Size: 1842
Fragments arranged by size:
1842 1455 1051 627 325 310 230 217
76
BanII G_rGCy'C
Cuts at: 1391 3452 1391
Size: 2061 4072
BbeI G_GCGC'C
Cuts at: 2101 2101
Size: 6133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2853 3102 928
Size: 971 235 719 249 3959
Fragments arranged by size:
3959 971 719 249 235
BbvCI CC'TCA_GC
Cuts at: 2817 2817
Size: 6133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6128 6128
Size: 6133
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2423 4194 5350 1790
Size: 633 1771 1156 2573
Fragments arranged by size:
2573 1771 1156 633
BmgBI CAC'GTC
Cuts at: 1318 2851 1318
Size: 1533 4600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3864 4822 442
Size: 416 53 83 399 24 2447 958 1753
Fragments arranged by size:
2447 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2289 2967 4793 1239
Size: 1050 678 1826 2579
Fragments arranged by size:
2579 1826 1050 678
Bpu10I CC'TnA_GC
Cuts at: 2817 3159 2817
Size: 342 5791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3288 4258 5123 5421 3288
Size: 970 865 298 4000
Fragments arranged by size:
4000 970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 4796 882
Size: 617 940 2357 2219
Fragments arranged by size:
2357 2219 940 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 3924 493
Size: 711 1059 1661 2702
Fragments arranged by size:
2702 1661 1059 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2098 3940 4322 4957
Size: 53 83 186 936 565 1842 382 635
Cuts at: 4957 275
Size: 1451
Fragments arranged by size:
1842 1451 936 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2226 4566 4964 5238 5385 5796 2226
Size: 2340 398 274 147 411 2563
Fragments arranged by size:
2563 2340 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5908
Fragments arranged by size:
5908 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2557 2805 3185 3248 3260 1186
Size: 1371 248 380 63 12 4059
Fragments arranged by size:
4059 1371 380 248 63 12
BseYI C'CCAG_C
Cuts at: 2458 5336 5832 2458
Size: 2878 496 2759
Fragments arranged by size:
2878 2759 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2423 4194 4279 5350 2423
Size: 1771 85 1071 3206
Fragments arranged by size:
3206 1771 1071 85
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2423 3452 4194 4279 5350 1391
Size: 399 633 1029 742 85 1071 2174
Fragments arranged by size:
2174 1071 1029 742 633 399 85
BspEI T'CCGG_A
Cuts at: 2226 5796 2226
Size: 3570 2563
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3247 3488 844
Size: 2403 241 3489
Fragments arranged by size:
3489 2403 241
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2250 2547 4020 1394
Size: 429 187 240 297 1473 3507
Fragments arranged by size:
3507 1473 429 297 240 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4628 4802 65
Size: 1183 3380 174 1396
Fragments arranged by size:
3380 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 4777 4964 1700
Size: 110 349 2618 187 2869
Fragments arranged by size:
2869 2618 349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 3210 4193 5205 3210
Size: 983 1012 4138
Fragments arranged by size:
4138 1012 983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3206 794
Size: 2412 3721
BstBI TT'CG_AA
Cuts at: 2136 4937 2136
Size: 2801 3332
BstEII G'GTnAC_C
Cuts at: 1184 2155 3116 1184
Size: 971 961 4201
Fragments arranged by size:
4201 971 961
BstYI r'GATC_y
Cuts at: 1972 2241 2741 2795 3463 3830 4215 4232 5684
Size: 269 500 54 668 367 385 17 1452
Cuts at: 5684 5770 5782 6128 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 668 500 385 367 346 269
86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2207 3426 513
Size: 552 1142 1219 3220
Fragments arranged by size:
3220 1219 1142 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 3322 3649 4443 4463 3322
Size: 327 794 20 4992
Fragments arranged by size:
4992 794 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 3580 4469
Size: 54 1425 50 275 18 385 1365 889
Cuts at: 4469 6098 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1365 889 385 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2822 3120 4063 1353
Size: 695 774 298 943 3423
Fragments arranged by size:
3423 943 774 695 298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2289 2393 2846 2967 4196 4793
Size: 393 536 121 104 453 121 1229 597
Cuts at: 4793 5580 5848 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1229 787 597 536 453 393 268
121 121 104
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2736 3125 3287 2150
Size: 586 389 162 4996
Fragments arranged by size:
4996 586 389 162
EcoO109I rG'GnC_Cy
Cuts at: 2080 2080
Size: 6133
EcoRI G'AATT_C
Cuts at: 2854 2899 3442 3820 2854
Size: 45 543 378 5167
Fragments arranged by size:
5167 543 378 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2600 2600
Size: 6133
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 2022 2101 5280 1678
Size: 344 79 3179 2531
Fragments arranged by size:
3179 2531 344 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 2795 2827 3166
Size: 32 135 32 1190 32 1 32 339
Cuts at: 3166 3198 4774 4806 4848 4880 1373
Size: 32 1576 32 42 32 2626
Fragments arranged by size:
2626 1576 1190 339 135 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 2787 3477 3723 669
Size: 988 281 750 99 690 246 3079
Fragments arranged by size:
3079 988 750 690 281 246 99
HindIII A'AGCT_T
Cuts at: 748 2715 748
Size: 1967 4166
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KasI G'GCGC_C
Cuts at: 2097 2097
Size: 6133
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 2773 5247 5431 1305
Size: 1281 187 2474 184 2007
Fragments arranged by size:
2474 2007 1281 187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2217 10
Size: 54 1425 343 385 3926
Fragments arranged by size:
3926 1425 385 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4091 4450 4609 518
Size: 648 2925 359 159 2042
Fragments arranged by size:
2925 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2209 3549 4226 5374 5619 1606
Size: 603 1340 677 1148 245 2120
Fragments arranged by size:
2120 1340 1148 677 603 245
NarI GG'CG_CC
Cuts at: 2098 2098
Size: 6133
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5581
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 3491 5036 1132
Size: 97 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2080
Size: 6133
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 3255 3485 830
Size: 2425 230 3478
Fragments arranged by size:
3478 2425 230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
SacII CC_GC'GG
Cuts at: 2210 2210
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SanDI GG'GwC_CC
Cuts at: 2080 2080
Size: 6133
SapI GCTCTTCn'nnn_
Cuts at: 2048 2048
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SfcI C'TryA_G
Cuts at: 826 1045 3147 3251 3481 4616 5297 5488 826
Size: 219 2102 104 230 1135 681 191 1471
Fragments arranged by size:
2102 1471 1135 681 230 219 191 104
SfoI GGC'GCC
Cuts at: 2099 2099
Size: 6133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3303 4237 5138 5400 820
Size: 197 926 1360 934 901 262 1553
Fragments arranged by size:
1553 1360 934 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 2701 1517
Size: 1184 4949
StyI C'CwwG_G
Cuts at: 513 1065 3356 3572 513
Size: 552 2291 216 3074
Fragments arranged by size:
3074 2291 552 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4160 4177 4330 4515 4960 325
Size: 1255 2580 17 153 185 445 1498
Fragments arranged by size:
2580 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2509 4379 6091
Size: 274 80 33 51 1443 532 1870 1712
Cuts at: 6091 96
Size: 138
Fragments arranged by size:
1870 1712 1443 532 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6093 6125 6093
Size: 32 6101
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 2913 4262 2913
Size: 1349 4784
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SexAI
SfiI SrfI SwaI XcmI