Restriction map for
pFN21AA1153
(Circular) (Six-base) MAPSORT of: pFN21AA1153.seq Check: 4550 from: 1 to: 5905
pFN21AA1153 5905 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2224 3248 1937
Size: 287 1024 4594
Fragments arranged by size:
4594 1024 287
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3152 3968 5352 5620 1632
Size: 1185 335 816 1384 268 1917
Fragments arranged by size:
1917 1384 1185 816 335 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2627 4804 5781 1203
Size: 1424 2177 977 1327
Fragments arranged by size:
2177 1424 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3133 1184
Size: 1949 3956
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2697 2729 2697
Size: 32 5873
AlwNI CAG_nnn'CTG
Cuts at: 3648 5220 3648
Size: 1572 4333
ApaI G_GGCC'C
Cuts at: 2033 2033
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2423 2771 3214 3440 3592 5701 1493
Size: 930 348 443 226 152 2109 1697
Fragments arranged by size:
2109 1697 930 443 348 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 4681 618
Size: 325 217 627 1439 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 2033 3163 3224 1391
Size: 624 18 1130 61 4072
Fragments arranged by size:
4072 1130 624 61 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2789 2919 2958 928
Size: 971 890 130 39 3875
Fragments arranged by size:
3875 971 890 130 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2279 3966 5122 1790
Size: 243 246 1687 1156 2573
Fragments arranged by size:
2573 1687 1156 246 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2721 2900 4565 1239
Size: 1482 179 1665 2579
Fragments arranged by size:
2579 1665 1482 179
Bpu10I CC'TnA_GC
Cuts at: 2014 2014
Size: 5905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2310 2964 4030 4895 5193 2310
Size: 654 1066 865 298 3022
Fragments arranged by size:
3022 1066 865 654 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2046 4568 882
Size: 617 547 2522 2219
Fragments arranged by size:
2522 2219 617 547
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2697 2727 1178
Size: 30 165 30 1294 30 4356
Fragments arranged by size:
4356 1294 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2718 3061 3135 1186
Size: 1532 343 74 3956
Fragments arranged by size:
3956 1532 343 74
BseYI C'CCAG_C
Cuts at: 2644 5108 5604 2644
Size: 2464 496 2945
Fragments arranged by size:
2945 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2652 1163
Size: 573 916 4416
Fragments arranged by size:
4416 916 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3966 4051 5122 3966
Size: 85 1071 4749
Fragments arranged by size:
4749 1071 85
BsmI GAATG_Cn'
Cuts at: 3181 3416 3509 3181
Size: 235 93 5577
Fragments arranged by size:
5577 235 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2033 2279 3163 3224 3966 4051
Size: 399 225 18 246 884 61 742 85
Cuts at: 4051 5122 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 884 742 399 246 225 85
61 18
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3089 3792 1394
Size: 429 1266 703 3507
Fragments arranged by size:
3507 1266 703 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2658 4400 4574 65
Size: 1183 1410 1742 174 1396
Fragments arranged by size:
1742 1410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 2145 4709 2145
Size: 2564 3341
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 2189 2189
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2983 3235 3602 3987 4004 5456 5542 5554
Size: 1011 252 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 252 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4414
Fragments arranged by size:
4414 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 3421 4215 4235 3421
Size: 794 20 5091
Fragments arranged by size:
5091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 2107 3209 2107
Size: 1102 4803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2179 3352 4241
Size: 54 1425 50 275 18 349 1173 889
Cuts at: 4241 5870 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1173 889 349 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2234 2241 3051 3835 1353
Size: 881 7 810 784 3423
Fragments arranged by size:
3423 881 810 784 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2721 2817 2900 3152 3968 4565 5352
Size: 393 1089 96 83 252 816 597 787
Cuts at: 5352 5620 1239
Size: 268 1524
Fragments arranged by size:
1524 1089 816 787 597 393 268 252
96 83
EcoNI CCTnn'n_nnAGG
Cuts at: 2703 2703
Size: 5905
EcoO109I rG'GnC_Cy
Cuts at: 2708 2929 2999 2708
Size: 221 70 5614
Fragments arranged by size:
5614 221 70
EcoRI G'AATT_C
Cuts at: 2771 3214 3592 2771
Size: 443 378 5084
Fragments arranged by size:
5084 443 378
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 4546 4578 4620
Size: 32 135 32 722 32 2220 32 42
Cuts at: 4620 4652 1373
Size: 32 2626
Fragments arranged by size:
2626 2220 722 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2580 3249 3495 669
Size: 988 281 642 669 246 3079
Fragments arranged by size:
3079 988 669 642 281 246
HindIII A'AGCT_T
Cuts at: 748 2340 748
Size: 1592 4313
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2181 10
Size: 54 1425 343 349 3734
Fragments arranged by size:
3734 1425 349 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 3863 4222 4381 518
Size: 648 1021 1676 359 159 2042
Fragments arranged by size:
2042 1676 1021 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 3321 3998 5146 5391 1606
Size: 519 1196 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 677 519 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4414
Fragments arranged by size:
4414 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2733 3157 3263 4808 1132
Size: 97 1504 424 106 1545 2229
Fragments arranged by size:
2229 1545 1504 424 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2047 2047
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2697 2729 3974 4006 5487 5519 2697
Size: 32 1245 32 1481 32 3083
Fragments arranged by size:
3083 1481 1245 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2708 2708
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2029 2029
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2260 3257 830
Size: 1430 997 3478
Fragments arranged by size:
3478 1430 997
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SapI GCTCTTCn'nnn_
Cuts at: 2241 3051 2241
Size: 810 5095
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2225 2256 3253 4388 5069 5260 826
Size: 219 1180 31 997 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 997 681 219 191 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2325 2979 4009 4910 5172 820
Size: 197 926 382 654 1030 901 262 1553
Fragments arranged by size:
1553 1030 926 901 654 382 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2004 3344 513
Size: 552 939 1340 3074
Fragments arranged by size:
3074 1340 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 2961 3105 3932 3949 4102 4287
Size: 1255 1056 325 144 827 17 153 185
Cuts at: 4287 4732 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1056 827 445 325 185 153
144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2113 2399 4151
Size: 274 80 33 51 1443 136 286 1752
Cuts at: 4151 5863 96
Size: 1712 138
Fragments arranged by size:
1752 1712 1443 286 274 138 136 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 2376 2812 4034 2376
Size: 436 1222 4247
Fragments arranged by size:
4247 1222 436
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI