Restriction map for
pFN21AA1144
(Circular) (Six-base) MAPSORT of: pFN21AA1144.seq Check: 8624 from: 1 to: 6136
pFN21AA1144 6136 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2199 3946 278
Size: 53 83 186 936 663 1747 2468
Fragments arranged by size:
2468 1747 936 663 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2290 2554 4199 5583 5851 1632
Size: 658 264 1645 1384 268 1917
Fragments arranged by size:
1917 1645 1384 658 268 264
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 2028 3433 5035 6012 1203
Size: 825 1405 1602 977 1327
Fragments arranged by size:
1602 1405 1327 977 825
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 2800 4865 2800
Size: 2065 4071
AleI CACnn'nnGTG
Cuts at: 1166 2828 1166
Size: 1662 4474
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2666 2698 2666
Size: 32 6104
AlwNI CAG_nnn'CTG
Cuts at: 2020 2893 3879 5451 2020
Size: 873 986 1572 2705
Fragments arranged by size:
2705 1572 986 873
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 3445 3671 3823 5932 1493
Size: 1952 226 152 2109 1697
Fragments arranged by size:
2109 1952 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2393 3461 1870
Size: 73 177 273 1068 4545
Fragments arranged by size:
4545 1068 273 177 73
AvrII C'CTAG_G
Cuts at: 2678 2678
Size: 6136
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3258 3457 4912 618
Size: 325 217 627 1471 199 1455 1842
Fragments arranged by size:
1842 1471 1455 627 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2178 2235 2394 3401 3455 1391
Size: 787 57 159 1007 54 4072
Fragments arranged by size:
4072 1007 787 159 57 54
BbeI G_GCGC'C
Cuts at: 3262 3262
Size: 6136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2290 2433 2560 928
Size: 971 391 143 127 4504
Fragments arranged by size:
4504 971 391 143 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4327 4361 1903
Size: 34 2390 34 3678
Fragments arranged by size:
3678 2390 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4747 136
Size: 107 122 71 631 605 3075 1525
Fragments arranged by size:
3075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 2286 2558 3553 2286
Size: 272 995 4869
Fragments arranged by size:
4869 995 272
Bme1580I G_kGCm'C
Cuts at: 1790 4197 5353 1790
Size: 2407 1156 2573
Fragments arranged by size:
2573 2407 1156
BmgBI CAC'GTC
Cuts at: 1318 2029 2278 2439 1318
Size: 711 249 161 5015
Fragments arranged by size:
5015 711 249 161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 3867 4825
Size: 416 53 83 399 24 918 1532 958
Cuts at: 4825 442
Size: 1753
Fragments arranged by size:
1753 1532 958 918 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 4796 1239
Size: 953 2604 2579
Fragments arranged by size:
2604 2579 953
Bpu10I CC'TnA_GC
Cuts at: 2229 2229
Size: 6136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3293 4261 5126 5424 2747
Size: 546 968 865 298 3459
Fragments arranged by size:
3459 968 865 546 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4799 882
Size: 617 3300 2219
Fragments arranged by size:
3300 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2245 3434 3927 493
Size: 711 1041 1189 493 2702
Fragments arranged by size:
2702 1189 1041 711 493
BsaBI GATnn'nnATC
Cuts at: 2063 2906 3026 6130 2063
Size: 843 120 3104 2069
Fragments arranged by size:
3104 2069 843 120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2196 2475 3259 3943
Size: 53 83 186 936 663 279 784 684
Cuts at: 3943 4325 4960 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 784 684 663 635 382 279
186 83 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 2462 2492 1178
Size: 30 165 30 903 30 126 30 4822
Fragments arranged by size:
4822 903 165 126 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 3077 1186
Size: 915 976 4245
Fragments arranged by size:
4245 976 915
BseYI C'CCAG_C
Cuts at: 5339 5835 5339
Size: 496 5640
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5563
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2221 3586 4347 5375 1540
Size: 275 186 220 1365 761 1028 2301
Fragments arranged by size:
2301 1365 1028 761 275 220 186
BsiHKAI G_wGCw'C
Cuts at: 2178 2235 2435 2700 2855 3401 4197 4282 5353
Size: 57 200 265 155 546 796 85 1071
Cuts at: 5353 2178
Size: 2961
Fragments arranged by size:
2961 1071 796 546 265 200 155 85
57
BsmI GAATG_Cn'
Cuts at: 2782 3647 3740 2782
Size: 865 93 5178
Fragments arranged by size:
5178 865 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2117 2117
Size: 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2235 2394 2435 2700 2855 3401
Size: 399 388 57 159 41 265 155 546
Cuts at: 3401 3455 4197 4282 5353 1391
Size: 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 399 388 265 159
155 85 57 54 41
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3491 844
Size: 2647 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2952 4023 1394
Size: 429 1129 1071 3507
Fragments arranged by size:
3507 1129 1071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4631 4805 65
Size: 1183 1458 1925 174 1396
Fragments arranged by size:
1925 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2264 3255 4780 4967 1700
Size: 110 199 255 991 1525 187 2869
Fragments arranged by size:
2869 1525 991 255 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 3203 4196 5208 3203
Size: 993 1012 4131
Fragments arranged by size:
4131 1012 993
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2107 794
Size: 1313 4823
BstBI TT'CG_AA
Cuts at: 2685 4940 2685
Size: 2255 3881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstXI CCAn_nnnn'nTGG
Cuts at: 2590 2590
Size: 6136
BstYI r'GATC_y
Cuts at: 1972 2058 2454 2907 3466 3833 4218 4235 5687
Size: 86 396 453 559 367 385 17 1452
Cuts at: 5687 5773 5785 6131 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 559 453 396 385 367 346
86 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2894 2894
Size: 6136
BtgI C'CryG_G
Cuts at: 513 1065 2374 3015 3036 3156 513
Size: 552 1309 641 21 120 3493
Fragments arranged by size:
3493 1309 641 552 120 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2196 487
Size: 683 56 970 4427
Fragments arranged by size:
4427 970 683 56
BtsI GCAGTG_nn'
Cuts at: 3652 4446 4466 3652
Size: 794 20 5322
Fragments arranged by size:
5322 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3420 3440 3420
Size: 20 6116
DraIII CAC_nnn'GTG
Cuts at: 2616 3407 2616
Size: 791 5345
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2377 2733
Size: 54 1425 50 275 18 181 366 356
Cuts at: 2733 2928 3078 3583 4472 6101 8
Size: 195 150 505 889 1629 43
Fragments arranged by size:
1629 1425 889 505 366 356 275 195
181 150 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 4066 1353
Size: 887 1826 3423
Fragments arranged by size:
3423 1826 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4723 5097 5243 387
Size: 1064 138 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2176 2233 3399 2176
Size: 57 1166 4913
Fragments arranged by size:
4913 1166 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2290 2554 4199 4796 5583 5851
Size: 393 560 98 264 1645 597 787 268
Cuts at: 5851 1239
Size: 1524
Fragments arranged by size:
1645 1524 787 597 560 393 268 264
98
EcoO109I rG'GnC_Cy
Cuts at: 2674 2890 3073 3319 2674
Size: 216 183 246 5491
Fragments arranged by size:
5491 246 216 183
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FspI TGC'GCA
Cuts at: 3287 4642 3287
Size: 1355 4781
HaeII r_GCGC'y
Cuts at: 1678 2534 3262 5283 1678
Size: 856 728 2021 2531
Fragments arranged by size:
2531 2021 856 728
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2786 2818 4777 4809 4851
Size: 32 135 32 1214 32 1959 32 42
Cuts at: 4851 4883 1373
Size: 32 2626
Fragments arranged by size:
2626 1959 1214 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2270 748
Size: 1522 4614
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KasI G'GCGC_C
Cuts at: 3258 3258
Size: 6136
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2013 5250 5434 1305
Size: 708 3237 184 2007
Fragments arranged by size:
3237 2007 708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2735 2930 3080 10
Size: 54 1425 343 903 195 150 3066
Fragments arranged by size:
3066 1425 903 343 195 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2588 2612 2828 3203 4094 4453
Size: 648 903 519 24 216 375 891 359
Cuts at: 4453 4612 518
Size: 159 2042
Fragments arranged by size:
2042 903 891 648 519 375 359 216
159 24
MspA1I CmG'CkG
Cuts at: 1606 2203 2521 3291 3552 4229 5377 5622 1606
Size: 597 318 770 261 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 677 597 318 261 245
NarI GG'CG_CC
Cuts at: 3259 3259
Size: 6136
NcoI C'CATG_G
Cuts at: 513 1065 2374 513
Size: 552 1309 4275
Fragments arranged by size:
4275 1309 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3494 5039 1132
Size: 97 2265 1545 2229
Fragments arranged by size:
2265 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2956 3149 1348
Size: 1608 193 4335
Fragments arranged by size:
4335 1608 193
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2674 2890 3319 2674
Size: 216 429 5491
Fragments arranged by size:
5491 429 216
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PstI C_TGCA'G
Cuts at: 830 2363 3488 830
Size: 1533 1125 3478
Fragments arranged by size:
3478 1533 1125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2521 3291 2521
Size: 770 5366
SacI G_AGCT'C
Cuts at: 2178 2235 3401 2178
Size: 57 1166 4913
Fragments arranged by size:
4913 1166 57
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SapI GCTCTTCn'nnn_
Cuts at: 2240 2240
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 2324 4384 6096 2324
Size: 2060 1712 2364
Fragments arranged by size:
2364 2060 1712
SexAI A'CCwGG_T
Cuts at: 2838 2838
Size: 6136
SfcI C'TryA_G
Cuts at: 826 1045 2359 2365 3484 4619 5300 5491 826
Size: 219 1314 6 1119 1135 681 191 1471
Fragments arranged by size:
1471 1314 1135 1119 681 219 191 6
SfoI GGC'GCC
Cuts at: 3260 3260
Size: 6136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3272 4240 5141 5403 820
Size: 197 926 819 510 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 819 510 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2374 2678 3575 513
Size: 552 1309 304 897 3074
Fragments arranged by size:
3074 1309 897 552 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3316 4163 4180 4333 4518 4963 325
Size: 1255 1736 847 17 153 185 445 1498
Fragments arranged by size:
1736 1498 1255 847 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 4382 6094
Size: 274 80 33 51 1443 345 2060 1712
Cuts at: 6094 96
Size: 138
Fragments arranged by size:
2060 1712 1443 345 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 2195 1316
Size: 879 5257
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2663 2743 3034 2663
Size: 80 291 5765
Fragments arranged by size:
5765 291 80
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 4265 4265
Size: 6136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2197 3944 276
Size: 53 83 186 936 663 1747 2468
Fragments arranged by size:
2468 1747 936 663 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbvCI BfrBI BplI
BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SrfI SwaI