Restriction map for
pFN21AA1141
(Circular) (Six-base) MAPSORT of: pFN21AA1141.seq Check: 1571 from: 1 to: 7318
pFN21AA1141 7318 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5128 278
Size: 53 83 186 936 3592 2468
Fragments arranged by size:
3592 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4661 1937
Size: 2724 4594
Acc65I G'GTAC_C
Cuts at: 4639 4639
Size: 7318
AclI AA'CG_TT
Cuts at: 1563 4133 4540 5445 5818 1563
Size: 2570 407 905 373 3063
Fragments arranged by size:
3063 2570 905 407 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2641 3223 3614 3896 3952 5381 6765 7033
Size: 1009 582 391 282 56 1429 1384 268
Cuts at: 7033 1632
Size: 1917
Fragments arranged by size:
1917 1429 1384 1009 582 391 282 268
56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7121
AflIII A'CryG_T
Cuts at: 1203 2091 6217 7194 1203
Size: 888 4126 977 1327
Fragments arranged by size:
4126 1327 977 888
AgeI A'CCGG_T
Cuts at: 6149 6149
Size: 7318
AhdI GACnn_n'nnGTC
Cuts at: 6047 6047
Size: 7318
AleI CACnn'nnGTG
Cuts at: 1166 3606 4179 1166
Size: 2440 573 4305
Fragments arranged by size:
4305 2440 573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7318
AlwNI CAG_nnn'CTG
Cuts at: 4501 5061 6633 4501
Size: 560 1572 5186
Fragments arranged by size:
5186 1572 560
ApaLI G'TGCA_C
Cuts at: 3978 5375 6531 3978
Size: 1397 1156 4765
Fragments arranged by size:
4765 1397 1156
ApoI r'AATT_y
Cuts at: 1493 2015 2792 3357 4170 4254 4422 4627 4853
Size: 522 777 565 813 84 168 205 226
Cuts at: 4853 5005 7114 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 813 777 565 522 226 205
168 152 84
AseI AT'TA_AT
Cuts at: 160 1032 5872 160
Size: 872 4840 1606
Fragments arranged by size:
4840 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 4567 4643 1870
Size: 73 129 2495 76 4545
Fragments arranged by size:
4545 2495 129 76 73
BamHI G'GATC_C
Cuts at: 4648 5015 4648
Size: 367 6951
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3015 4560 4639 6094 618
Size: 325 217 627 1228 1545 79 1455 1842
Fragments arranged by size:
1842 1545 1455 1228 627 325 217 79
BanII G_rGCy'C
Cuts at: 1391 4148 4316 4637 1391
Size: 2757 168 321 4072
Fragments arranged by size:
4072 2757 321 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 4042 928
Size: 971 491 1652 4204
Fragments arranged by size:
4204 1652 971 491
BbvCI CC'TCA_GC
Cuts at: 2010 4574 2010
Size: 2564 4754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5509 5543 1903
Size: 34 3572 34 3678
Fragments arranged by size:
3678 3572 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2778 5210 6420 2778
Size: 2432 1210 3676
Fragments arranged by size:
3676 2432 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5929 136
Size: 107 122 71 631 605 4257 1525
Fragments arranged by size:
4257 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2185 3651 7313 2185
Size: 1466 3662 2190
Fragments arranged by size:
3662 2190 1466
BlpI GC'TnA_GC
Cuts at: 4233 4735 4233
Size: 502 6816
Bme1580I G_kGCm'C
Cuts at: 1790 3982 4486 5379 6535 1790
Size: 2192 504 893 1156 2573
Fragments arranged by size:
2573 2192 1156 893 504
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2071 2901 4356
Size: 416 53 83 399 24 654 830 1455
Cuts at: 4356 5049 6007 442
Size: 693 958 1753
Fragments arranged by size:
1753 1455 958 830 693 654 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 7318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2886 3219 4032 4284 5978 1239
Size: 971 676 333 813 252 1694 2579
Fragments arranged by size:
2579 1694 971 813 676 333 252
Bpu10I CC'TnA_GC
Cuts at: 2010 4574 2010
Size: 2564 4754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 3097 5443 6308 6606 2012
Size: 1085 2346 865 298 2724
Fragments arranged by size:
2724 2346 1085 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 5981 882
Size: 617 735 3747 2219
Fragments arranged by size:
3747 2219 735 617
BsaAI yAC'GTr
Cuts at: 493 1204 5109 493
Size: 711 3905 2702
Fragments arranged by size:
3905 2702 711
BsaBI GATnn'nnATC
Cuts at: 3501 7312 3501
Size: 3811 3507
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5125 5507 6142 275
Size: 53 83 186 936 3592 382 635 1451
Fragments arranged by size:
3592 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2745 4332 5751 6149 6423 6570 6981 2745
Size: 1587 1419 398 274 147 411 3082
Fragments arranged by size:
3082 1587 1419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 2656 2686 1178
Size: 30 165 30 744 30 479 30 5810
Fragments arranged by size:
5810 744 479 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2244 3218 1186
Size: 1058 974 5286
Fragments arranged by size:
5286 1058 974
BseYI C'CCAG_C
Cuts at: 2679 6521 7017 2679
Size: 3842 496 2980
Fragments arranged by size:
3842 2980 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6745
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4768 5529 6557 1540
Size: 275 186 2767 761 1028 2301
Fragments arranged by size:
2767 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3936 3982 4148 4316 5379 5464 6535 3936
Size: 46 166 168 1063 85 1071 4719
Fragments arranged by size:
4719 1071 1063 168 166 85 46
BsmI GAATG_Cn'
Cuts at: 2447 2755 2981 3398 3482 3959 4127 4295 4829
Size: 308 226 417 84 477 168 168 534
Cuts at: 4829 4922 2447
Size: 93 4843
Fragments arranged by size:
4843 534 477 417 308 226 168 168
93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3936 3982 4148 4316 4486 4637 5379
Size: 399 2146 46 166 168 170 151 742
Cuts at: 5379 5464 6535 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 2146 1071 742 399 170 168 166
151 85 46
BspEI T'CCGG_A
Cuts at: 6981 6981
Size: 7318
BspHI T'CATG_A
Cuts at: 2452 2911 5207 6937 2452
Size: 459 2296 1730 2833
Fragments arranged by size:
2833 2296 1730 459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4673 844
Size: 3829 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5205 1394
Size: 429 3382 3507
Fragments arranged by size:
3507 3382 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5813 5987 65
Size: 1183 4565 174 1396
Fragments arranged by size:
4565 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3327 3411 5962 6149 1700
Size: 110 1517 84 2551 187 2869
Fragments arranged by size:
2869 2551 1517 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 4591 96
Size: 4495 2823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7318
BssSI C'ACGA_G
Cuts at: 3602 3743 5378 6390 3602
Size: 141 1635 1012 4530
Fragments arranged by size:
4530 1635 1012 141
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7318
BstBI TT'CG_AA
Cuts at: 2274 6122 2274
Size: 3848 3470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7318
BstXI CCAn_nnnn'nTGG
Cuts at: 2174 2174
Size: 7318
BstYI r'GATC_y
Cuts at: 1972 2185 2997 3024 3261 3651 4648 5015 5400
Size: 213 812 27 237 390 997 367 385
Cuts at: 5400 5417 6869 6955 6967 7313 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 997 812 390 385 367 346
237 213 86 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2436 2493 2508 513
Size: 552 939 432 57 15 5323
Fragments arranged by size:
5323 939 552 432 57 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6579
Fragments arranged by size:
6579 683 56
BtsI GCAGTG_nn'
Cuts at: 2185 3310 3898 4834 5628 5648 2185
Size: 1125 588 936 794 20 3855
Fragments arranged by size:
3855 1125 936 794 588 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7283
DraI TTT'AAA
Cuts at: 3943 4027 4622 3943
Size: 84 595 6639
Fragments arranged by size:
6639 595 84
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6325 809
Size: 507 5009 1802
Fragments arranged by size:
5009 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3805 4765 5654
Size: 54 1425 50 275 18 1975 960 889
Cuts at: 5654 7283 8
Size: 1629 43
Fragments arranged by size:
1975 1629 1425 960 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4765 1537
Size: 275 2953 4090
Fragments arranged by size:
4090 2953 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3195 5248 1353
Size: 782 1060 2053 3423
Fragments arranged by size:
3423 2053 1060 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4232 5905 6279 6425 387
Size: 1064 138 2643 1673 374 146 1280
Fragments arranged by size:
2643 1673 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4146 4314 4146
Size: 168 7150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2641 2886 3219 3223 3614 3896
Size: 393 578 431 245 333 4 391 282
Cuts at: 3896 3952 4032 4284 5381 5978 6765 7033 1239
Size: 56 80 252 1097 597 787 268 1524
Fragments arranged by size:
1524 1097 787 597 578 431 393 391
333 282 268 252 245 80 56 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2199 2199
Size: 7318
EcoO109I rG'GnC_Cy
Cuts at: 2125 2125
Size: 7318
EcoRI G'AATT_C
Cuts at: 3357 4170 4254 4422 4627 5005 3357
Size: 813 84 168 205 378 5670
Fragments arranged by size:
5670 813 378 205 168 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 7318
FspI TGC'GCA
Cuts at: 5824 5824
Size: 7318
HaeII r_GCGC'y
Cuts at: 1678 6465 1678
Size: 4787 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2656 2688 2942
Size: 32 135 32 722 32 330 32 254
Cuts at: 2942 2974 3016 3048 3418 3450 3643 3675 4513
Size: 32 42 32 370 32 193 32 838
Cuts at: 4513 4545 5959 5991 6033 6065 1373
Size: 32 1414 32 42 32 2626
Fragments arranged by size:
2626 1414 838 722 370 330 254 193
135 42 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4538 4662 4908 669
Size: 988 281 2600 124 246 3079
Fragments arranged by size:
3079 2600 988 281 246 124
HindIII A'AGCT_T
Cuts at: 748 2653 4467 748
Size: 1905 1814 3599
Fragments arranged by size:
3599 1905 1814
HpaI GTT'AAC
Cuts at: 4908 4908
Size: 7318
KpnI G_GTAC'C
Cuts at: 4643 4643
Size: 7318
MfeI C'AATT_G
Cuts at: 2116 4917 2116
Size: 2801 4517
MluI A'CGCG_T
Cuts at: 7194 7194
Size: 7318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 2610 3118 6432 6616 1305
Size: 786 519 508 3314 184 2007
Fragments arranged by size:
3314 2007 786 519 508 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3807 10
Size: 54 1425 343 1975 3521
Fragments arranged by size:
3521 1975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2632 2842 3342 3550 3606 4179
Size: 648 1006 460 210 500 208 56 573
Cuts at: 4179 4587 5276 5635 5794 518
Size: 408 689 359 159 2042
Fragments arranged by size:
2042 1006 689 648 573 500 460 408
359 210 208 159 56
MspA1I CmG'CkG
Cuts at: 1606 4734 5411 6559 6804 1606
Size: 3128 677 1148 245 2120
Fragments arranged by size:
3128 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3413 3413
Size: 7318
NcoI C'CATG_G
Cuts at: 513 1065 2004 2508 513
Size: 552 939 504 5323
Fragments arranged by size:
5323 939 552 504
NdeI CA'TA_TG
Cuts at: 387 3547 387
Size: 3160 4158
NgoMIV G'CCGG_C
Cuts at: 3411 3411
Size: 7318
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7318
NotI GC'GGCC_GC
Cuts at: 4765 4765
Size: 7318
NspI r_CATG'y
Cuts at: 1132 1229 4676 6221 1132
Size: 97 3447 1545 2229
Fragments arranged by size:
3447 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7318
PciI A'CATG_T
Cuts at: 6217 6217
Size: 7318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7318
PmeI GTTT'AAAC
Cuts at: 4622 4622
Size: 7318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5387 5419 6900 6932 5387
Size: 32 1481 32 5773
Fragments arranged by size:
5773 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2125
Size: 7318
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7318
PsiI TTA'TAA
Cuts at: 3213 4888 3213
Size: 1675 5643
PspXI vC'TCGA_Gb
Cuts at: 4567 4567
Size: 7318
PstI C_TGCA'G
Cuts at: 830 3725 4670 830
Size: 2895 945 3478
Fragments arranged by size:
3478 2895 945
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7318
SacI G_AGCT'C
Cuts at: 4148 4316 4148
Size: 168 7150
SalI G'TCGA_C
Cuts at: 1936 4660 1936
Size: 2724 4594
SapI GCTCTTCn'nnn_
Cuts at: 3195 3195
Size: 7318
SbfI CC_TGCA'GG
Cuts at: 4670 4670
Size: 7318
ScaI AGT'ACT
Cuts at: 3814 5566 7278 3814
Size: 1752 1712 3854
Fragments arranged by size:
3854 1752 1712
SexAI A'CCwGG_T
Cuts at: 2363 2363
Size: 7318
SfcI C'TryA_G
Cuts at: 826 1045 3370 3463 3721 4360 4666 5801 6482
Size: 219 2325 93 258 639 306 1135 681
Cuts at: 6482 6673 826
Size: 191 1471
Fragments arranged by size:
2325 1471 1135 681 639 306 258 219
191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7318
SmaI CCC'GGG
Cuts at: 1872 4645 1872
Size: 2773 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 3076 4567 5422 6323 6585
Size: 197 926 84 1049 1491 855 901 262
Cuts at: 6585 820
Size: 1553
Fragments arranged by size:
1553 1491 1049 926 901 855 262 197
84
SnaBI TAC'GTA
Cuts at: 493 5109 493
Size: 4616 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7318
SphI G_CATG'C
Cuts at: 1132 4676 1132
Size: 3544 3774
SspI AAT'ATT
Cuts at: 5 52 5242 5
Size: 47 5190 2081
Fragments arranged by size:
5190 2081 47
StuI AGG'CCT
Cuts at: 1517 3320 4217 1517
Size: 1803 897 4618
Fragments arranged by size:
4618 1803 897
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2313 2508 4757 513
Size: 552 939 48 261 195 2249 3074
Fragments arranged by size:
3074 2249 939 552 261 195 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2184 2916 2920 4070 4322 4529 5345
Size: 1255 604 732 4 1150 252 207 816
Cuts at: 5345 5362 5515 5700 6145 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1150 816 732 604 445 252
207 185 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2834 3812 4591
Size: 274 80 33 51 1443 857 978 779
Cuts at: 4591 5564 7276 96
Size: 973 1712 138
Fragments arranged by size:
1712 1443 978 973 857 779 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3624 3656 7278 7310 3624
Size: 32 3622 32 3632
Fragments arranged by size:
3632 3622 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2472 4046 1316
Size: 1156 1574 4588
Fragments arranged by size:
4588 1574 1156
XbaI T'CTAG_A
Cuts at: 3845 4654 3845
Size: 809 6509
XhoI C'TCGA_G
Cuts at: 1943 4567 1943
Size: 2624 4694
XmaI C'CCGG_G
Cuts at: 1870 4643 1870
Size: 2773 4545
XmnI GAAnn'nnTTC
Cuts at: 2397 2654 3236 3320 4133 4217 4301 5447 2397
Size: 257 582 84 813 84 84 1146 4268
Fragments arranged by size:
4268 1146 813 582 257 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5126 276
Size: 53 83 186 936 3592 2468
Fragments arranged by size:
3592 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PsrI PvuII RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI