Restriction map for
pFN21AA1140
(Circular) (Six-base) MAPSORT of: pFN21AA1140.seq Check: 6037 from: 1 to: 6922
pFN21AA1140 6922 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3366 3469 3366
Size: 103 6819
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4265 1937
Size: 2328 4594
Acc65I G'GTAC_C
Cuts at: 2898 4243 2898
Size: 1345 5577
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2423 3185 3344 3764 4985 6369 6637 1632
Size: 791 762 159 420 1221 1384 268 1917
Fragments arranged by size:
1917 1384 1221 791 762 420 268 159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6725
AflIII A'CryG_T
Cuts at: 1203 2042 5821 6798 1203
Size: 839 3779 977 1327
Fragments arranged by size:
3779 1327 977 839
AgeI A'CCGG_T
Cuts at: 5753 5753
Size: 6922
AhdI GACnn_n'nnGTC
Cuts at: 2834 5651 2834
Size: 2817 4105
AleI CACnn'nnGTG
Cuts at: 1166 3464 1166
Size: 2298 4624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3539 3859 1184
Size: 2355 320 4247
Fragments arranged by size:
4247 2355 320
AlwNI CAG_nnn'CTG
Cuts at: 2617 3208 3226 3484 3544 3662 4102 4665 6237
Size: 591 18 258 60 118 440 563 1572
Cuts at: 6237 2617
Size: 3302
Fragments arranged by size:
3302 1572 591 563 440 258 118 60
18
ApaI G_GGCC'C
Cuts at: 3755 3755
Size: 6922
ApaLI G'TGCA_C
Cuts at: 4979 6135 4979
Size: 1156 5766
ApoI r'AATT_y
Cuts at: 1493 2885 4231 4457 4609 6718 1493
Size: 1392 1346 226 152 2109 1697
Fragments arranged by size:
2109 1697 1392 1346 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5476 160
Size: 872 4444 1606
Fragments arranged by size:
4444 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 2718 4247 1870
Size: 73 775 1529 4545
Fragments arranged by size:
4545 1529 775 73
BamHI G'GATC_C
Cuts at: 4252 4619 4252
Size: 367 6555
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2740 2898 3229 4243 5698
Size: 325 217 627 953 158 331 1014 1455
Cuts at: 5698 618
Size: 1842
Fragments arranged by size:
1842 1455 1014 953 627 331 325 217
158
BanII G_rGCy'C
Cuts at: 1391 2355 2439 2555 3220 3755 4221 4241 1391
Size: 964 84 116 665 535 466 20 4072
Fragments arranged by size:
4072 964 665 535 466 116 84 20
BbeI G_GCGC'C
Cuts at: 3233 3233
Size: 6922
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2300 928
Size: 971 401 5550
Fragments arranged by size:
5550 971 401
BbvCI CC'TCA_GC
Cuts at: 3527 3912 3527
Size: 385 6537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5113 5147 1903
Size: 34 50 34 3092 34 3678
Fragments arranged by size:
3678 3092 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4814 6024 4814
Size: 1210 5712
BclI T'GATC_A
Cuts at: 1548 2235 3072 1548
Size: 687 837 5398
Fragments arranged by size:
5398 837 687
BfrBI ATG'CAT
Cuts at: 3994 3994
Size: 6922
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2612 5533 136
Size: 107 122 71 631 605 940 2921 1525
Fragments arranged by size:
2921 1525 940 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3288 3783 6917 3288
Size: 495 3134 3293
Fragments arranged by size:
3293 3134 495
BlpI GC'TnA_GC
Cuts at: 2732 3219 3801 4339 2732
Size: 487 582 538 5315
Fragments arranged by size:
5315 582 538 487
Bme1580I G_kGCm'C
Cuts at: 1790 3000 3755 4983 6139 1790
Size: 1210 755 1228 1156 2573
Fragments arranged by size:
2573 1228 1210 1156 755
BmgBI CAC'GTC
Cuts at: 1318 2313 1318
Size: 995 5927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3228 4653 5611
Size: 416 53 83 399 24 1811 1425 958
Cuts at: 5611 442
Size: 1753
Fragments arranged by size:
1811 1753 1425 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6922
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2666 2272
Size: 394 6528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2569 2876 3230 3277 3494 3722 3950
Size: 1124 206 307 354 47 217 228 228
Cuts at: 3950 4193 5582 1239
Size: 243 1389 2579
Fragments arranged by size:
2579 1389 1124 354 307 243 228 228
217 206 47
Bpu10I CC'TnA_GC
Cuts at: 2268 2804 3527 3912 2268
Size: 536 723 385 5278
Fragments arranged by size:
5278 723 536 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3812 4187 5047 5912 6210 3812
Size: 375 860 865 298 4524
Fragments arranged by size:
4524 865 860 375 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 3134 5585 882
Size: 617 957 678 2451 2219
Fragments arranged by size:
2451 2219 957 678 617
BsaAI yAC'GTr
Cuts at: 493 1204 4080 4713 493
Size: 711 2876 633 2702
Fragments arranged by size:
2876 2702 711 633
BsaBI GATnn'nnATC
Cuts at: 3071 6916 3071
Size: 3845 3077
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3156 3230 4729 5111
Size: 53 83 186 936 1623 74 1499 382
Cuts at: 5111 5746 275
Size: 635 1451
Fragments arranged by size:
1623 1499 1451 936 635 382 186 83
74 53
BsaWI w'CCGG_w
Cuts at: 2760 3189 5355 5753 6027 6174 6585 2760
Size: 429 2166 398 274 147 411 3097
Fragments arranged by size:
3097 2166 429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3539 3569 3650 3680 3698
Size: 30 165 30 2136 30 81 30 18
Cuts at: 3698 3728 1178
Size: 30 4372
Fragments arranged by size:
4372 2136 165 81 30 30 30 30
30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 2552 2606 2681 2684 2768 3719 3947
Size: 1054 312 54 75 3 84 951 228
Cuts at: 3947 1186
Size: 4161
Fragments arranged by size:
4161 1054 951 312 228 84 75 54
3
BseYI C'CCAG_C
Cuts at: 3558 3562 4021 6125 6621 3558
Size: 4 459 2104 496 3859
Fragments arranged by size:
3859 2104 496 459 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 1163
Size: 573 771 5578
Fragments arranged by size:
5578 771 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5133 6161 1540
Size: 275 186 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2355 2439 3057 3435 4221 4983 5068 6139 2355
Size: 84 618 378 786 762 85 1071 3138
Fragments arranged by size:
3138 1071 786 762 618 378 85 84
BsmI GAATG_Cn'
Cuts at: 2785 4433 4526 2785
Size: 1648 93 5181
Fragments arranged by size:
5181 1648 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2355 2439 2555 3000 3057 3220 3435
Size: 399 565 84 116 445 57 163 215
Cuts at: 3435 3755 4221 4241 4983 5068 6139 1391
Size: 320 466 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 565 466 445 399 320
215 163 116 85 84 57 20
BspEI T'CCGG_A
Cuts at: 6585 6585
Size: 6922
BspHI T'CATG_A
Cuts at: 4811 6541 4811
Size: 1730 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3366 3469 3521 3827 4277 844
Size: 2522 103 52 306 450 3489
Fragments arranged by size:
3489 2522 450 306 103 52
BsrBI CCG'CTC
Cuts at: 1394 1823 4809 1394
Size: 429 2986 3507
Fragments arranged by size:
3507 2986 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5417 5591 65
Size: 1183 4169 174 1396
Fragments arranged by size:
4169 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3903 5566 5753 1700
Size: 110 2093 1663 187 2869
Fragments arranged by size:
2869 2093 1663 187 110
BsrGI T'GTAC_A
Cuts at: 96 3546 3948 96
Size: 3450 402 3070
Fragments arranged by size:
3450 3070 402
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 3506 4085 4982 5994 3506
Size: 579 897 1012 4434
Fragments arranged by size:
4434 1012 897 579
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6922
BstBI TT'CG_AA
Cuts at: 5726 5726
Size: 6922
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 3301 2506
Size: 795 6127
BstYI r'GATC_y
Cuts at: 1972 3288 3783 4252 4619 5004 5021 6473 6559
Size: 1316 495 469 367 385 17 1452 86
Cuts at: 6559 6571 6917 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1316 495 469 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2048 2709 2916 3300 4062 513
Size: 552 983 661 207 384 762 3373
Fragments arranged by size:
3373 983 762 661 552 384 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2179 3670 487
Size: 683 56 953 1491 3739
Fragments arranged by size:
3739 1491 953 683 56
BtsI GCAGTG_nn'
Cuts at: 3197 3365 4438 5232 5252 3197
Size: 168 1073 794 20 4867
Fragments arranged by size:
4867 1073 794 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6922
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 4226 4226
Size: 6922
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3245 5929 809
Size: 507 1929 2684 1802
Fragments arranged by size:
2684 1929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2496 3006 3420
Size: 54 1425 50 275 18 666 510 414
Cuts at: 3420 4369 5258 6887 8
Size: 949 889 1629 43
Fragments arranged by size:
1629 1425 949 889 666 510 414 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 1537
Size: 275 2557 4090
Fragments arranged by size:
4090 2557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3377 3739 3872 4852 1353
Size: 2024 362 133 980 3423
Fragments arranged by size:
3423 2024 980 362 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2198 5509 5883 6029 387
Size: 1064 138 609 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 609 374 146 138
EcoICRI GAG'CTC
Cuts at: 2353 2437 4219 2353
Size: 84 1782 5056
Fragments arranged by size:
5056 1782 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2423 2569 2876 3185 3230 3277
Size: 393 731 60 146 307 309 45 47
Cuts at: 3277 3344 3494 3722 3764 3950 4193 4985 5582
Size: 67 150 228 42 186 243 792 597
Cuts at: 5582 6369 6637 1239
Size: 787 268 1524
Fragments arranged by size:
1524 792 787 731 597 393 309 307
268 243 228 186 150 146 67 60
47 45 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 2534 3083 3107 3575 3746 3932 4106 2405
Size: 129 549 24 468 171 186 174 5221
Fragments arranged by size:
5221 549 468 186 174 171 129 24
EcoO109I rG'GnC_Cy
Cuts at: 2151 2253 2515 2614 3027 3319 3598 3751 3752
Size: 102 262 99 413 292 279 153 1
Cuts at: 3752 4099 2151
Size: 347 4974
Fragments arranged by size:
4974 413 347 292 279 262 153 102
99 1
EcoRI G'AATT_C
Cuts at: 4231 4609 4231
Size: 378 6544
FspI TGC'GCA
Cuts at: 3986 5428 3986
Size: 1442 5480
FspAI rTGC'GCAy
Cuts at: 3986 3986
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 2388 3233 3962 6069 1678
Size: 710 845 729 2107 2531
Fragments arranged by size:
2531 2107 845 729 710
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2820 2852 3644
Size: 32 135 32 457 32 759 32 792
Cuts at: 3644 3650 3676 3682 3698 3730 4000 4032 5563
Size: 6 26 6 16 32 270 32 1531
Cuts at: 5563 5595 5637 5669 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 792 759 457 270 135 42
32 32 32 32 32 32 32 32
26 16 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3415 4147 4266 4512 669
Size: 988 281 1477 732 119 246 3079
Fragments arranged by size:
3079 1477 988 732 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6922
HpaI GTT'AAC
Cuts at: 4512 4512
Size: 6922
KasI G'GCGC_C
Cuts at: 3229 3229
Size: 6922
KpnI G_GTAC'C
Cuts at: 2902 4247 2902
Size: 1345 5577
MfeI C'AATT_G
Cuts at: 4521 4521
Size: 6922
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2066 3307 6036 6220 1305
Size: 761 1241 2729 184 2007
Fragments arranged by size:
2729 2007 1241 761 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3422 10
Size: 54 1425 343 1590 3510
Fragments arranged by size:
3510 1590 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2014 2047 2634 3068 3396 3464 4880
Size: 648 848 33 587 434 328 68 1416
Cuts at: 4880 5239 5398 518
Size: 359 159 2042
Fragments arranged by size:
2042 1416 848 648 587 434 359 328
159 68 33
MspA1I CmG'CkG
Cuts at: 1606 3226 3271 3310 3562 3763 3946 4338 5015
Size: 1620 45 39 252 201 183 392 677
Cuts at: 5015 6163 6408 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1620 1148 677 392 252 245 201
183 45 39
NaeI GCC'GGC
Cuts at: 3905 3905
Size: 6922
NarI GG'CG_CC
Cuts at: 3230 3230
Size: 6922
NcoI C'CATG_G
Cuts at: 513 1065 2048 2709 2916 3300 513
Size: 552 983 661 207 384 4135
Fragments arranged by size:
4135 983 661 552 384 207
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6922
NgoMIV G'CCGG_C
Cuts at: 3903 3903
Size: 6922
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6922
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NsiI A_TGCA'T
Cuts at: 3996 3996
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 2019 2046 2111 3401 3628 4280 5825
Size: 97 790 27 65 1290 227 652 1545
Cuts at: 5825 1132
Size: 2229
Fragments arranged by size:
2229 1545 1290 790 652 227 97 65
27
PasI CC'CwG_GG
Cuts at: 1348 4123 1348
Size: 2775 4147
PciI A'CATG_T
Cuts at: 2042 5821 2042
Size: 3779 3143
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3361 1108
Size: 2253 4669
PfoI T'CCnGG_A
Cuts at: 2258 2364 2439 3849 2258
Size: 106 75 1410 5331
Fragments arranged by size:
5331 1410 106 75
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 6504 6536 4991
Size: 32 1481 32 5377
Fragments arranged by size:
5377 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2151 2515 3027 2151
Size: 364 512 6046
Fragments arranged by size:
6046 512 364
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PspOMI G'GGCC_C
Cuts at: 3751 3751
Size: 6922
PstI C_TGCA'G
Cuts at: 830 2159 2283 3147 3204 3270 3315 3518 3795
Size: 1329 124 864 57 66 45 203 277
Cuts at: 3795 4119 4274 830
Size: 324 155 3478
Fragments arranged by size:
3478 1329 864 324 277 203 155 124
66 57 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
PvuII CAG'CTG
Cuts at: 3226 3271 3310 3562 3763 3946 3226
Size: 45 39 252 201 183 6202
Fragments arranged by size:
6202 252 201 183 45 39
SacI G_AGCT'C
Cuts at: 2355 2439 4221 2355
Size: 84 1782 5056
Fragments arranged by size:
5056 1782 84
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SanDI GG'GwC_CC
Cuts at: 3027 3027
Size: 6922
SapI GCTCTTCn'nnn_
Cuts at: 3377 3377
Size: 6922
SbfI CC_TGCA'GG
Cuts at: 2159 2283 3147 4274 2159
Size: 124 864 1127 4807
Fragments arranged by size:
4807 1127 864 124
ScaI AGT'ACT
Cuts at: 3888 5170 6882 3888
Size: 1282 1712 3928
Fragments arranged by size:
3928 1712 1282
SexAI A'CCwGG_T
Cuts at: 2901 2901
Size: 6922
SfcI C'TryA_G
Cuts at: 826 1045 2155 2279 3136 3143 3200 3266 3311
Size: 219 1110 124 857 7 57 66 45
Cuts at: 3311 3514 3791 4115 4270 5405 6086 6277 826
Size: 203 277 324 155 1135 681 191 1471
Fragments arranged by size:
1471 1135 1110 857 681 324 277 219
203 191 155 124 66 57 45 7
SfoI GGC'GCC
Cuts at: 3231 3231
Size: 6922
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 4249 1872
Size: 2377 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2718 3827 4166 5026 5927 6189
Size: 197 926 775 1109 339 860 901 262
Cuts at: 6189 820
Size: 1553
Fragments arranged by size:
1553 1109 926 901 860 775 339 262
197
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 2111 3401 3628 4280 1132
Size: 979 1290 227 652 3774
Fragments arranged by size:
3774 1290 979 652 227
SspI AAT'ATT
Cuts at: 5 52 4846 5
Size: 47 4794 2081
Fragments arranged by size:
4794 2081 47
StuI AGG'CCT
Cuts at: 1517 2166 3570 1517
Size: 649 1404 4869
Fragments arranged by size:
4869 1404 649
StyI C'CwwG_G
Cuts at: 513 1065 2048 2661 2709 2916 3108 3300 3462
Size: 552 983 613 48 207 192 192 162
Cuts at: 3462 4361 513
Size: 899 3074
Fragments arranged by size:
3074 983 899 613 552 207 192 192
162 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3727 4949 4966 5119 5304 5749 325
Size: 1255 2147 1222 17 153 185 445 1498
Fragments arranged by size:
2147 1498 1255 1222 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3546 3886 3948
Size: 274 80 33 51 1443 1569 340 62
Cuts at: 3948 4189 5168 6880 96
Size: 241 979 1712 138
Fragments arranged by size:
1712 1569 1443 979 340 274 241 138
80 62 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6882 6914 6882
Size: 32 6890
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6922
XbaI T'CTAG_A
Cuts at: 4258 4258
Size: 6922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3115 3469 3115
Size: 354 6568
XhoI C'TCGA_G
Cuts at: 1943 2718 1943
Size: 775 6147
XmaI C'CCGG_G
Cuts at: 1870 4247 1870
Size: 2377 4545
XmnI GAAnn'nnTTC
Cuts at: 5051 5051
Size: 6922
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoRV FalI FseI NruI PacI PmlI
PspXI PsrI RsrII SacII SfiI SrfI SwaI