Restriction map for
pFN21AA1128
(Circular) (Six-base) MAPSORT of: pFN21AA1128.seq Check: 5228 from: 1 to: 7846
pFN21AA1128 7846 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3040 3040
Size: 7846
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5656 278
Size: 53 83 186 936 4120 2468
Fragments arranged by size:
4120 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4695 5189 1937
Size: 2758 494 4594
Fragments arranged by size:
4594 2758 494
Acc65I G'GTAC_C
Cuts at: 4798 5167 4798
Size: 369 7477
AclI AA'CG_TT
Cuts at: 1563 5973 6346 1563
Size: 4410 373 3063
Fragments arranged by size:
4410 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3367 4217 4596 4652 4871 5909 7293 7561
Size: 1735 850 379 56 219 1038 1384 268
Cuts at: 7561 1632
Size: 1917
Fragments arranged by size:
1917 1735 1384 1038 850 379 268 219
56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7649
AflIII A'CryG_T
Cuts at: 1203 2296 3041 3537 4480 4868 5143 6745 7722
Size: 1093 745 496 943 388 275 1602 977
Cuts at: 7722 1203
Size: 1327
Fragments arranged by size:
1602 1327 1093 977 943 745 496 388
275
AgeI A'CCGG_T
Cuts at: 6677 6677
Size: 7846
AhdI GACnn_n'nnGTC
Cuts at: 6575 6575
Size: 7846
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7846
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7846
AlwNI CAG_nnn'CTG
Cuts at: 4814 4930 5589 7161 4814
Size: 116 659 1572 5499
Fragments arranged by size:
5499 1572 659 116
ApaLI G'TGCA_C
Cuts at: 2229 4455 5903 7059 2229
Size: 2226 1448 1156 3016
Fragments arranged by size:
3016 2226 1448 1156
ApoI r'AATT_y
Cuts at: 1493 2263 2456 2692 2821 3025 4323 5155 5381
Size: 770 193 236 129 204 1298 832 226
Cuts at: 5381 5533 7642 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1298 832 770 236 226 204
193 152 129
AseI AT'TA_AT
Cuts at: 160 1032 2643 6400 160
Size: 872 1611 3757 1606
Fragments arranged by size:
3757 1611 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
AvaI C'yCGr_G
Cuts at: 1870 1943 4679 5171 1870
Size: 73 2736 492 4545
Fragments arranged by size:
4545 2736 492 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3160 3193 3160
Size: 33 7813
BamHI G'GATC_C
Cuts at: 5176 5543 5176
Size: 367 7479
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4798 5167 6622 618
Size: 325 217 627 3011 369 1455 1842
Fragments arranged by size:
3011 1842 1455 627 369 325 217
BanII G_rGCy'C
Cuts at: 1391 5165 1391
Size: 3774 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3246 3276 5117 928
Size: 971 1347 30 1841 3657
Fragments arranged by size:
3657 1841 1347 971 30
BbvCI CC'TCA_GC
Cuts at: 3824 3824
Size: 7846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6037 6071 1903
Size: 34 4100 34 3678
Fragments arranged by size:
4100 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5738 6948 5738
Size: 1210 6636
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7846
BfrBI ATG'CAT
Cuts at: 4444 4444
Size: 7846
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6457 136
Size: 107 122 71 631 605 4785 1525
Fragments arranged by size:
4785 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7841 7841
Size: 7846
BlpI GC'TnA_GC
Cuts at: 2611 5263 2611
Size: 2652 5194
Bme1580I G_kGCm'C
Cuts at: 1790 2233 4459 5907 7063 1790
Size: 443 2226 1448 1156 2573
Fragments arranged by size:
2573 2226 1448 1156 443
BmgBI CAC'GTC
Cuts at: 1318 4483 1318
Size: 3165 4681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5577 6535 442
Size: 416 53 83 399 24 4160 958 1753
Fragments arranged by size:
4160 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2622 3279 4336 4791 6506 1239
Size: 1383 657 1057 455 1715 2579
Fragments arranged by size:
2579 1715 1383 1057 657 455
Bpu10I CC'TnA_GC
Cuts at: 3072 3824 3072
Size: 752 7094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2292 3671 3886 4317 4590 5971 6836 7134 2292
Size: 1379 215 431 273 1381 865 298 3004
Fragments arranged by size:
3004 1381 1379 865 431 298 273 215
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6509 882
Size: 617 5010 2219
Fragments arranged by size:
5010 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5637 493
Size: 711 4433 2702
Fragments arranged by size:
4433 2702 711
BsaBI GATnn'nnATC
Cuts at: 3660 3678 7840 3660
Size: 18 4162 3666
Fragments arranged by size:
4162 3666 18
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5653 6035 6670 275
Size: 53 83 186 936 4120 382 635 1451
Fragments arranged by size:
4120 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4462 6279 6677 6951 7098 7509 4462
Size: 1817 398 274 147 411 4799
Fragments arranged by size:
4799 1817 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3418 3448 1178
Size: 30 165 30 2015 30 5576
Fragments arranged by size:
5576 2015 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 4320 1186
Size: 1240 1894 4712
Fragments arranged by size:
4712 1894 1240
BseYI C'CCAG_C
Cuts at: 7049 7545 7049
Size: 496 7350
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5296 6057 7085 1540
Size: 275 186 3295 761 1028 2301
Fragments arranged by size:
3295 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2233 4459 5907 5992 7063 2233
Size: 2226 1448 85 1071 3016
Fragments arranged by size:
3016 2226 1448 1071 85
BsmI GAATG_Cn'
Cuts at: 4430 5086 5357 5450 4430
Size: 656 271 93 6826
Fragments arranged by size:
6826 656 271 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4061 4312 4061
Size: 251 7595
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 4459 5165 5907 5992 7063 1391
Size: 399 443 2226 706 742 85 1071 2174
Fragments arranged by size:
2226 2174 1071 742 706 443 399 85
BspEI T'CCGG_A
Cuts at: 4462 7509 4462
Size: 3047 4799
BspHI T'CATG_A
Cuts at: 5735 7465 5735
Size: 1730 6116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3040 5201 844
Size: 2196 2161 3489
Fragments arranged by size:
3489 2196 2161
BsrBI CCG'CTC
Cuts at: 1394 1823 5733 1394
Size: 429 3910 3507
Fragments arranged by size:
3910 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2081 4085 4106 6341 6515 65
Size: 1183 833 2004 21 2235 174 1396
Fragments arranged by size:
2235 2004 1396 1183 833 174 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 6490 6677 1700
Size: 110 4680 187 2869
Fragments arranged by size:
4680 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3434 96
Size: 3338 4508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7846
BssSI C'ACGA_G
Cuts at: 5906 6918 5906
Size: 1012 6834
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 4425 794
Size: 1298 2333 4215
Fragments arranged by size:
4215 2333 1298
BstBI TT'CG_AA
Cuts at: 6650 6650
Size: 7846
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7846
BstXI CCAn_nnnn'nTGG
Cuts at: 3584 3584
Size: 7846
BstYI r'GATC_y
Cuts at: 1972 2749 3679 4215 5176 5543 5928 5945 7397
Size: 777 930 536 961 367 385 17 1452
Cuts at: 7397 7483 7495 7841 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 961 930 777 536 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3687 3765 4957 3687
Size: 78 1192 6576
Fragments arranged by size:
6576 1192 78
BtgI C'CryG_G
Cuts at: 513 1065 2004 3850 513
Size: 552 939 1846 4509
Fragments arranged by size:
4509 1846 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7107
Fragments arranged by size:
7107 683 56
BtsI GCAGTG_nn'
Cuts at: 4020 4426 5362 6156 6176 4020
Size: 406 936 794 20 5690
Fragments arranged by size:
5690 936 794 406 20
ClaI AT'CG_AT
Cuts at: 1554 5113 1554
Size: 3559 4287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2377 2412 4536 4571 529
Size: 35 1813 35 2124 35 3804
Fragments arranged by size:
3804 2124 1813 35 35 35
DraI TTT'AAA
Cuts at: 4773 5150 4773
Size: 377 7469
DraIII CAC_nnn'GTG
Cuts at: 2237 2237
Size: 7846
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6853 809
Size: 507 5537 1802
Fragments arranged by size:
5537 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5293 6182 7811
Size: 54 1425 50 275 18 3463 889 1629
Cuts at: 7811 8
Size: 43
Fragments arranged by size:
3463 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5293 1537
Size: 275 3481 4090
Fragments arranged by size:
4090 3481 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 3312 4509 4671 5776 1353
Size: 885 1074 1197 162 1105 3423
Fragments arranged by size:
3423 1197 1105 1074 885 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4719 4937 6433 6807 6953 387
Size: 1064 138 3130 218 1496 374 146 1280
Fragments arranged by size:
3130 1496 1280 1064 374 218 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2622 3279 3367 4217 4336 4596 4652
Size: 393 990 657 88 850 119 260 56
Cuts at: 4652 4791 4871 5909 6506 7293 7561 1239
Size: 139 80 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 990 850 787 657 597 393
268 260 139 119 88 80 56
EcoNI CCTnn'n_nnAGG
Cuts at: 2307 3768 2307
Size: 1461 6385
EcoRI G'AATT_C
Cuts at: 2692 4323 5155 5533 2692
Size: 1631 832 378 5005
Fragments arranged by size:
5005 1631 832 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2373 2373
Size: 7846
FspI TGC'GCA
Cuts at: 6352 6352
Size: 7846
HaeII r_GCGC'y
Cuts at: 1678 6993 1678
Size: 5315 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2491 2523 3290
Size: 32 135 32 593 32 294 32 767
Cuts at: 3290 3322 6487 6519 6561 6593 1373
Size: 32 3165 32 42 32 2626
Fragments arranged by size:
3165 2626 767 593 294 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5190 5436 669
Size: 988 281 3252 246 3079
Fragments arranged by size:
3252 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2608 4471 5008 748
Size: 1860 1863 537 3586
Fragments arranged by size:
3586 1863 1860 537
HpaI GTT'AAC
Cuts at: 5436 5436
Size: 7846
KpnI G_GTAC'C
Cuts at: 4802 5171 4802
Size: 369 7477
MfeI C'AATT_G
Cuts at: 5445 5445
Size: 7846
MluI A'CGCG_T
Cuts at: 7722 7722
Size: 7846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3300 4243 4693 6960 7144 1305
Size: 1995 943 450 2267 184 2007
Fragments arranged by size:
2267 2007 1995 943 450 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6024
Fragments arranged by size:
6024 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 3330 3538 3849 4068 5804 6163
Size: 648 1623 541 208 311 219 1736 359
Cuts at: 6163 6322 518
Size: 159 2042
Fragments arranged by size:
2042 1736 1623 648 541 359 311 219
208 159
MspA1I CmG'CkG
Cuts at: 1606 2549 5262 5939 7087 7332 1606
Size: 943 2713 677 1148 245 2120
Fragments arranged by size:
2713 2120 1148 943 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3850 513
Size: 552 939 1846 4509
Fragments arranged by size:
4509 1846 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7846
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7846
NotI GC'GGCC_GC
Cuts at: 5293 5293
Size: 7846
NsiI A_TGCA'T
Cuts at: 4446 4446
Size: 7846
NspI r_CATG'y
Cuts at: 1132 1229 2560 3045 3541 4444 4648 4872 5147
Size: 97 1331 485 496 903 204 224 275
Cuts at: 5147 5204 6749 1132
Size: 57 1545 2229
Fragments arranged by size:
2229 1545 1331 903 496 485 275 224
204 97 57
PasI CC'CwG_GG
Cuts at: 1348 4381 1348
Size: 3033 4813
PciI A'CATG_T
Cuts at: 3041 3537 4868 5143 6745 3041
Size: 496 1331 275 1602 4142
Fragments arranged by size:
4142 1602 1331 496 275
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2057 2996 4133 1108
Size: 949 939 1137 4821
Fragments arranged by size:
4821 1137 949 939
PmeI GTTT'AAAC
Cuts at: 5150 5150
Size: 7846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5915 5947 7428 7460 5915
Size: 32 1481 32 6301
Fragments arranged by size:
6301 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7846
PsiI TTA'TAA
Cuts at: 5416 5416
Size: 7846
PstI C_TGCA'G
Cuts at: 830 5198 830
Size: 4368 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7846
PvuII CAG'CTG
Cuts at: 2549 2549
Size: 7846
SalI G'TCGA_C
Cuts at: 1936 5188 1936
Size: 3252 4594
SapI GCTCTTCn'nnn_
Cuts at: 2238 4509 2238
Size: 2271 5575
SbfI CC_TGCA'GG
Cuts at: 5198 5198
Size: 7846
ScaI AGT'ACT
Cuts at: 2841 4338 6094 7806 2841
Size: 1497 1756 1712 2881
Fragments arranged by size:
2881 1756 1712 1497
SfcI C'TryA_G
Cuts at: 826 1045 2406 3060 3481 3883 4340 5194 6329
Size: 219 1361 654 421 402 457 854 1135
Cuts at: 6329 7010 7201 826
Size: 681 191 1471
Fragments arranged by size:
1471 1361 1135 854 681 654 457 421
402 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7846
SmaI CCC'GGG
Cuts at: 1872 5173 1872
Size: 3301 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 3650 3865 4332 4605 5950
Size: 197 926 364 1343 215 467 273 1345
Cuts at: 5950 6851 7113 820
Size: 901 262 1553
Fragments arranged by size:
1553 1345 1343 926 901 467 364 273
262 215 197
SnaBI TAC'GTA
Cuts at: 493 5637 493
Size: 5144 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7846
SphI G_CATG'C
Cuts at: 1132 2560 5204 1132
Size: 1428 2644 3774
Fragments arranged by size:
3774 2644 1428
SspI AAT'ATT
Cuts at: 5 52 5770 5
Size: 47 5718 2081
Fragments arranged by size:
5718 2081 47
StuI AGG'CCT
Cuts at: 1517 2566 2881 1517
Size: 1049 315 6482
Fragments arranged by size:
6482 1049 315
StyI C'CwwG_G
Cuts at: 513 1065 2004 3363 3850 5033 5285 513
Size: 552 939 1359 487 1183 252 3074
Fragments arranged by size:
3074 1359 1183 939 552 487 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3753 4394 5873 5890 6043 6228 6673
Size: 1255 2173 641 1479 17 153 185 445
Cuts at: 6673 325
Size: 1498
Fragments arranged by size:
2173 1498 1479 1255 641 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2078 2839 3434
Size: 274 80 33 51 1443 101 761 595
Cuts at: 3434 4336 4565 4688 6092 7804 96
Size: 902 229 123 1404 1712 138
Fragments arranged by size:
1712 1443 1404 902 761 595 274 229
138 123 101 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7806 7838 7806
Size: 32 7814
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7846
XbaI T'CTAG_A
Cuts at: 5182 5182
Size: 7846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3795 3800 3795
Size: 5 7841
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7846
XmaI C'CCGG_G
Cuts at: 1870 5171 1870
Size: 3301 4545
XmnI GAAnn'nnTTC
Cuts at: 2485 2596 2696 4081 5975 2485
Size: 111 100 1385 1894 4356
Fragments arranged by size:
4356 1894 1385 111 100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5654 276
Size: 53 83 186 936 4120 2468
Fragments arranged by size:
4120 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI
BstZ17I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI