Restriction map for
pFN21AA1121
(Circular) (Six-base) MAPSORT of: pFN21AA1121.seq Check: 7177 from: 1 to: 8764
pFN21AA1121 8764 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4929 4929
Size: 8764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2947 6574 278
Size: 53 83 186 936 1411 3627 2468
Fragments arranged by size:
3627 2468 1411 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2947 4417 4797 6107 1937
Size: 1010 1470 380 1310 4594
Fragments arranged by size:
4594 1470 1310 1010 380
Acc65I G'GTAC_C
Cuts at: 6085 6085
Size: 8764
AclI AA'CG_TT
Cuts at: 1563 4336 6891 7264 1563
Size: 2773 2555 373 3063
Fragments arranged by size:
3063 2773 2555 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 3053 4443 4667 5210 5277 5312 6827
Size: 956 465 1390 224 543 67 35 1515
Cuts at: 6827 8211 8479 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1515 1390 1384 956 543 465 268
224 67 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8567
AflIII A'CryG_T
Cuts at: 1203 3015 3660 4714 7663 8640 1203
Size: 1812 645 1054 2949 977 1327
Fragments arranged by size:
2949 1812 1327 1054 977 645
AgeI A'CCGG_T
Cuts at: 2478 7595 2478
Size: 5117 3647
AhdI GACnn_n'nnGTC
Cuts at: 2017 7493 2017
Size: 5476 3288
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2087 1184
Size: 903 7861
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2856 2888 3155 3187 2856
Size: 32 267 32 8433
Fragments arranged by size:
8433 267 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2461 4864 6507 8079 2461
Size: 2403 1643 1572 3146
Fragments arranged by size:
3146 2403 1643 1572
ApaLI G'TGCA_C
Cuts at: 6821 7977 6821
Size: 1156 7608
ApoI r'AATT_y
Cuts at: 1493 2723 2981 4004 4907 6073 6299 6451 8560
Size: 1230 258 1023 903 1166 226 152 2109
Cuts at: 8560 1493
Size: 1697
Fragments arranged by size:
2109 1697 1230 1166 1023 903 258 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7318 160
Size: 872 6286 1606
Fragments arranged by size:
6286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
AvaI C'yCGr_G
Cuts at: 1870 1943 2625 5678 6089 1870
Size: 73 682 3053 411 4545
Fragments arranged by size:
4545 3053 682 411 73
BamHI G'GATC_C
Cuts at: 6094 6461 6094
Size: 367 8397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2165 3335 4226 5143
Size: 325 217 627 372 6 1170 891 917
Cuts at: 5143 5189 6085 7540 618
Size: 46 896 1455 1842
Fragments arranged by size:
1842 1455 1170 917 896 891 627 372
325 217 46 6
BanII G_rGCy'C
Cuts at: 1391 2129 2711 3985 6083 1391
Size: 738 582 1274 2098 4072
Fragments arranged by size:
4072 2098 1274 738 582
BbeI G_GCGC'C
Cuts at: 2163 2169 4230 2163
Size: 6 2061 6697
Fragments arranged by size:
6697 2061 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2626 6054 6063 928
Size: 971 727 3428 9 3629
Fragments arranged by size:
3629 3428 971 727 9
BbvCI CC'TCA_GC
Cuts at: 2687 3942 2687
Size: 1255 7509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4170 4204 6955 6989 1903
Size: 34 50 34 2149 34 2751 34 3678
Fragments arranged by size:
3678 2751 2149 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3007 3091 6656 7866 3007
Size: 84 3565 1210 3905
Fragments arranged by size:
3905 3565 1210 84
BclI T'GATC_A
Cuts at: 1548 3304 5779 1548
Size: 1756 2475 4533
Fragments arranged by size:
4533 2475 1756
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2256 7375 136
Size: 107 122 71 631 605 584 5119 1525
Fragments arranged by size:
5119 1525 631 605 584 122 107 71
BglII A'GATC_T
Cuts at: 3523 5200 8759 3523
Size: 1677 3559 3528
Fragments arranged by size:
3559 3528 1677
BlpI GC'TnA_GC
Cuts at: 2705 6181 2705
Size: 3476 5288
Bme1580I G_kGCm'C
Cuts at: 1790 3338 3897 6825 7981 1790
Size: 1548 559 2928 1156 2573
Fragments arranged by size:
2928 2573 1548 1156 559
BmgBI CAC'GTC
Cuts at: 1318 5689 1318
Size: 4371 4393
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4481 6495 7453
Size: 416 53 83 399 24 3064 2014 958
Cuts at: 7453 442
Size: 1753
Fragments arranged by size:
3064 2014 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2257 3089 4812 7424 1239
Size: 1018 832 1723 2612 2579
Fragments arranged by size:
2612 2579 1723 1018 832
Bpu10I CC'TnA_GC
Cuts at: 2687 3942 2687
Size: 1255 7509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6889 7754 8052 6889
Size: 865 298 7601
Fragments arranged by size:
7601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7427 882
Size: 617 5928 2219
Fragments arranged by size:
5928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5704 6555 493
Size: 711 4500 851 2702
Fragments arranged by size:
4500 2702 851 711
BsaBI GATnn'nnATC
Cuts at: 8758 8758
Size: 8764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2160 2166 2944 3408
Size: 53 83 186 936 627 6 778 464
Cuts at: 3408 4227 5220 5697 6571 6953 7588 275
Size: 819 993 477 874 382 635 1451
Fragments arranged by size:
1451 993 936 874 819 778 635 627
477 464 382 186 83 53 6
BsaWI w'CCGG_w
Cuts at: 2478 5829 5958 7197 7595 7869 8016 8427 2478
Size: 3351 129 1239 398 274 147 411 2815
Fragments arranged by size:
3351 2815 1239 411 398 274 147 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2163 2193 2856 2886 3020
Size: 30 165 30 760 30 663 30 134
Cuts at: 3020 3050 3155 3185 3792 3822 3921 3951 5780
Size: 30 105 30 607 30 99 30 1829
Cuts at: 5780 5810 1178
Size: 30 4132
Fragments arranged by size:
4132 1829 760 663 607 165 134 105
99 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2069 2351 2354 2357 3041 3167 3300
Size: 880 3 282 3 3 684 126 133
Cuts at: 3300 4514 4709 5768 1186
Size: 1214 195 1059 4182
Fragments arranged by size:
4182 1214 1059 880 684 282 195 133
126 3 3 3
BseYI C'CCAG_C
Cuts at: 2108 2146 2243 2279 3750 7967 8463 2108
Size: 38 97 36 1471 4217 496 2409
Fragments arranged by size:
4217 2409 1471 496 97 38 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2205 2564 3665 3694 5231 5797 1163
Size: 573 469 359 1101 29 1537 566 4130
Fragments arranged by size:
4130 1537 1101 573 566 469 359 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 2266 2578 6214 6975 8003
Size: 275 186 132 133 312 3636 761 1028
Cuts at: 8003 1540
Size: 2301
Fragments arranged by size:
3636 2301 1028 761 312 275 186 133
132
BsiHKAI G_wGCw'C
Cuts at: 2076 2711 3404 3985 4701 6825 6910 7981 2076
Size: 635 693 581 716 2124 85 1071 2859
Fragments arranged by size:
2859 2124 1071 716 693 635 581 85
BsmI GAATG_Cn'
Cuts at: 3574 6275 6368 3574
Size: 2701 93 5970
Fragments arranged by size:
5970 2701 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4766 5972 4766
Size: 1206 7558
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2129 2711 3338 3404 3897 3985
Size: 399 286 53 582 627 66 493 88
Cuts at: 3985 4701 6083 6825 6910 7981 1391
Size: 716 1382 742 85 1071 2174
Fragments arranged by size:
2174 1382 1071 742 716 627 582 493
399 286 88 85 66 53
BspEI T'CCGG_A
Cuts at: 5958 8427 5958
Size: 2469 6295
BspHI T'CATG_A
Cuts at: 2532 6653 8383 2532
Size: 4121 1730 2913
Fragments arranged by size:
4121 2913 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3057 3637 4929 5253 6119 844
Size: 2213 580 1292 324 866 3489
Fragments arranged by size:
3489 2213 1292 866 580 324
BsrBI CCG'CTC
Cuts at: 1394 1823 2061 2323 5008 6651 1394
Size: 429 238 262 2685 1643 3507
Fragments arranged by size:
3507 2685 1643 429 262 238
BsrDI GCAATG_nn'
Cuts at: 65 1248 4906 7259 7433 65
Size: 1183 3658 2353 174 1396
Fragments arranged by size:
3658 2353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2132 2162 2265 2433 2478 3076 4614
Size: 110 322 30 103 168 45 598 1538
Cuts at: 4614 7408 7595 1700
Size: 2794 187 2869
Fragments arranged by size:
2869 2794 1538 598 322 187 168 110
103 45 30
BsrGI T'GTAC_A
Cuts at: 96 3018 5760 96
Size: 2922 2742 3100
Fragments arranged by size:
3100 2922 2742
BssHII G'CGCG_C
Cuts at: 1925 2094 2096 1925
Size: 169 2 8593
Fragments arranged by size:
8593 169 2
BssSI C'ACGA_G
Cuts at: 6824 7836 6824
Size: 1012 7752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8764
BstBI TT'CG_AA
Cuts at: 2862 7568 2862
Size: 4706 4058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8764
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 3007 4286 4836 5662 2425
Size: 582 1279 550 826 5527
Fragments arranged by size:
5527 1279 826 582 550
BstYI r'GATC_y
Cuts at: 1972 3523 4960 5200 6094 6461 6846 6863 8315
Size: 1551 1437 240 894 367 385 17 1452
Cuts at: 8315 8401 8413 8759 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1551 1452 1437 894 385 367 346
240 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3702 3702
Size: 8764
BtgI C'CryG_G
Cuts at: 513 1065 2740 2756 3870 4285 513
Size: 552 1675 16 1114 415 4992
Fragments arranged by size:
4992 1675 1114 552 415 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2374 6054 487
Size: 683 56 1148 3680 3197
Fragments arranged by size:
3680 3197 1148 683 56
BtsI GCAGTG_nn'
Cuts at: 3365 6280 7074 7094 3365
Size: 2915 794 20 5035
Fragments arranged by size:
5035 2915 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3258 3293 529
Size: 35 2694 35 6000
Fragments arranged by size:
6000 2694 35 35
DraI TTT'AAA
Cuts at: 6068 6068
Size: 8764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4780 7771 809
Size: 507 3464 2991 1802
Fragments arranged by size:
3464 2991 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2263 2718
Size: 54 1425 50 275 18 300 133 455
Cuts at: 2718 3057 3769 4123 5691 6211 7100 8729 8
Size: 339 712 354 1568 520 889 1629 43
Fragments arranged by size:
1629 1568 1425 889 712 520 455 354
339 300 275 133 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2130 2263 6211 1537
Size: 275 318 133 3948 4090
Fragments arranged by size:
4090 3948 318 275 133
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 2348 2564 2648 2742 3608 4018 4578
Size: 954 41 216 84 94 866 410 560
Cuts at: 4578 5256 6694 1353
Size: 678 1438 3423
Fragments arranged by size:
3423 1438 954 866 678 560 410 216
94 84 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2602 5705 7351 7725 7871 387
Size: 1064 138 1013 3103 1646 374 146 1280
Fragments arranged by size:
3103 1646 1280 1064 1013 374 146 138
EcoICRI GAG'CTC
Cuts at: 2709 3983 2709
Size: 1274 7490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2257 2588 3053 3089 4443 4667 4812
Size: 393 625 331 465 36 1354 224 145
Cuts at: 4812 5210 5277 5312 6827 7424 8211 8479 1239
Size: 398 67 35 1515 597 787 268 1524
Fragments arranged by size:
1524 1515 1354 787 625 597 465 398
393 331 268 224 145 67 36 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2690 2690
Size: 8764
EcoO109I rG'GnC_Cy
Cuts at: 2752 5242 5917 2752
Size: 2490 675 5599
Fragments arranged by size:
5599 2490 675
EcoRI G'AATT_C
Cuts at: 6073 6451 6073
Size: 378 8386
FseI GG_CCGG'CC
Cuts at: 2269 2269
Size: 8764
FspI TGC'GCA
Cuts at: 2663 7270 2663
Size: 4607 4157
HaeII r_GCGC'y
Cuts at: 1678 2142 2163 2169 4230 7911 1678
Size: 464 21 6 2061 3681 2531
Fragments arranged by size:
3681 2531 2061 464 21 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 3020 3052 4997
Size: 32 135 32 431 32 985 32 1945
Cuts at: 4997 5029 5168 5200 7405 7437 7479 7511 1373
Size: 32 139 32 2205 32 42 32 2626
Fragments arranged by size:
2626 2205 1945 985 431 139 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3046 4418 4549 5104 5823 6108
Size: 988 281 1108 1372 131 555 719 285
Cuts at: 6108 6354 669
Size: 246 3079
Fragments arranged by size:
3079 1372 1108 988 719 555 285 281
246 131
HindIII A'AGCT_T
Cuts at: 748 4637 748
Size: 3889 4875
HpaI GTT'AAC
Cuts at: 5104 6354 5104
Size: 1250 7514
KasI G'GCGC_C
Cuts at: 2159 2165 4226 2159
Size: 6 2061 6697
Fragments arranged by size:
6697 2061 6
KpnI G_GTAC'C
Cuts at: 6089 6089
Size: 8764
MfeI C'AATT_G
Cuts at: 3716 4977 6363 3716
Size: 1261 1386 6117
Fragments arranged by size:
6117 1386 1261
MluI A'CGCG_T
Cuts at: 8640 8640
Size: 8764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 3399 4041 7878 8062 1305
Size: 993 1101 642 3837 184 2007
Fragments arranged by size:
3837 2007 1101 993 642 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2720 3059 4125 10
Size: 54 1425 343 888 339 1066 4649
Fragments arranged by size:
4649 1425 1066 888 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3143 4284 5149 5468 5804 6026
Size: 648 1257 720 1141 865 319 336 222
Cuts at: 6026 6722 7081 7240 518
Size: 696 359 159 2042
Fragments arranged by size:
2042 1257 1141 865 720 696 648 359
336 319 222 159
MspA1I CmG'CkG
Cuts at: 1606 2205 2217 2365 2461 2758 2893 3374 3946
Size: 599 12 148 96 297 135 481 572
Cuts at: 3946 4861 6180 6857 8005 8250 1606
Size: 915 1319 677 1148 245 2120
Fragments arranged by size:
2120 1319 1148 915 677 599 572 481
297 245 148 135 96 12
NaeI GCC'GGC
Cuts at: 2134 2164 2267 2435 2134
Size: 30 103 168 8463
Fragments arranged by size:
8463 168 103 30
NarI GG'CG_CC
Cuts at: 2160 2166 4227 2160
Size: 6 2061 6697
Fragments arranged by size:
6697 2061 6
NcoI C'CATG_G
Cuts at: 513 1065 4285 513
Size: 552 3220 4992
Fragments arranged by size:
4992 3220 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8764
NgoMIV G'CCGG_C
Cuts at: 2132 2162 2265 2433 2132
Size: 30 103 168 8463
Fragments arranged by size:
8463 168 103 30
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8764
NotI GC'GGCC_GC
Cuts at: 6211 6211
Size: 8764
NspI r_CATG'y
Cuts at: 1132 1229 3019 3076 4718 4769 6122 7667 1132
Size: 97 1790 57 1642 51 1353 1545 2229
Fragments arranged by size:
2229 1790 1642 1545 1353 97 57 51
PasI CC'CwG_GG
Cuts at: 1348 3340 1348
Size: 1992 6772
PciI A'CATG_T
Cuts at: 3015 4714 7663 3015
Size: 1699 2949 4116
Fragments arranged by size:
4116 2949 1699
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2515 1108
Size: 1407 7357
PfoI T'CCnGG_A
Cuts at: 2711 5919 2711
Size: 3208 5556
PmeI GTTT'AAAC
Cuts at: 6068 6068
Size: 8764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2856 2888 3155 3187 6833 6865 8346 8378 2856
Size: 32 267 32 3646 32 1481 32 3242
Fragments arranged by size:
3646 3242 1481 267 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2752 5242 5917 2752
Size: 2490 675 5599
Fragments arranged by size:
5599 2490 675
PshAI GACnn'nnGTC
Cuts at: 1613 3905 5711 1613
Size: 2292 1806 4666
Fragments arranged by size:
4666 2292 1806
PsiI TTA'TAA
Cuts at: 6334 6334
Size: 8764
PstI C_TGCA'G
Cuts at: 830 2331 2364 2466 6018 6116 830
Size: 1501 33 102 3552 98 3478
Fragments arranged by size:
3552 3478 1501 102 98 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8764
PvuII CAG'CTG
Cuts at: 2365 2461 2893 3374 3946 4861 2365
Size: 96 432 481 572 915 6268
Fragments arranged by size:
6268 915 572 481 432 96
RsrII CG'GwC_CG
Cuts at: 5827 5827
Size: 8764
SacI G_AGCT'C
Cuts at: 2711 3985 2711
Size: 1274 7490
SacII CC_GC'GG
Cuts at: 2759 2759
Size: 8764
SalI G'TCGA_C
Cuts at: 1936 4416 6106 1936
Size: 2480 1690 4594
Fragments arranged by size:
4594 2480 1690
SapI GCTCTTCn'nnn_
Cuts at: 2564 2564
Size: 8764
SbfI CC_TGCA'GG
Cuts at: 6116 6116
Size: 8764
ScaI AGT'ACT
Cuts at: 7012 8724 7012
Size: 1712 7052
SexAI A'CCwGG_T
Cuts at: 5816 5816
Size: 8764
SfcI C'TryA_G
Cuts at: 826 1045 2327 2360 2462 3296 4255 5893 6014
Size: 219 1282 33 102 834 959 1638 121
Cuts at: 6014 6112 7247 7928 8119 826
Size: 98 1135 681 191 1471
Fragments arranged by size:
1638 1471 1282 1135 959 834 681 219
191 121 102 98 33
SfoI GGC'GCC
Cuts at: 2161 2167 4228 2161
Size: 6 2061 6697
Fragments arranged by size:
6697 2061 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8764
SgrAI Cr'CCGG_yG
Cuts at: 1700 2162 1700
Size: 462 8302
SmaI CCC'GGG
Cuts at: 1872 2627 5680 6091 1872
Size: 755 3053 411 4545
Fragments arranged by size:
4545 3053 755 411
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6868 7769 8031 820
Size: 197 926 4925 901 262 1553
Fragments arranged by size:
4925 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6555 493
Size: 6062 2702
SpeI A'CTAG_T
Cuts at: 152 4666 152
Size: 4514 4250
SphI G_CATG'C
Cuts at: 1132 3076 4769 6122 1132
Size: 1944 1693 1353 3774
Fragments arranged by size:
3774 1944 1693 1353
SspI AAT'ATT
Cuts at: 5 52 6688 5
Size: 47 6636 2081
Fragments arranged by size:
6636 2081 47
StuI AGG'CCT
Cuts at: 1517 3231 4297 1517
Size: 1714 1066 5984
Fragments arranged by size:
5984 1714 1066
StyI C'CwwG_G
Cuts at: 513 1065 3226 3924 4285 6203 513
Size: 552 2161 698 361 1918 3074
Fragments arranged by size:
3074 2161 1918 698 552 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 3353 6791 6808 6961 7146 7591
Size: 1255 591 1182 3438 17 153 185 445
Cuts at: 7591 325
Size: 1498
Fragments arranged by size:
3438 1498 1255 1182 591 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 3657 4135
Size: 274 80 33 51 1443 1041 639 478
Cuts at: 4135 5760 7010 8722 96
Size: 1625 1250 1712 138
Fragments arranged by size:
1712 1625 1443 1250 1041 639 478 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 3116 3148 3792 3824 8724 8756 2163
Size: 32 921 32 644 32 4900 32 2171
Fragments arranged by size:
4900 2171 921 644 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8764
XbaI T'CTAG_A
Cuts at: 6100 6100
Size: 8764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 2843 3142 2287
Size: 556 299 7909
Fragments arranged by size:
7909 556 299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8764
XmaI C'CCGG_G
Cuts at: 1870 2625 5678 6089 1870
Size: 755 3053 411 4545
Fragments arranged by size:
4545 3053 755 411
XmnI GAAnn'nnTTC
Cuts at: 3389 4680 6893 3389
Size: 1291 2213 5260
Fragments arranged by size:
5260 2213 1291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2945 6572 276
Size: 53 83 186 936 1411 3627 2468
Fragments arranged by size:
3627 2468 1411 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI
PmlI PspOMI PspXI PsrI SanDI SfiI SrfI SwaI