Restriction map for
pFN21AA1116
(Circular) (Six-base) MAPSORT of: pFN21AA1116.seq Check: 4052 from: 1 to: 8518
pFN21AA1116 8518 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6328 278
Size: 53 83 186 936 4792 2468
Fragments arranged by size:
4792 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4977 5089 5861 1937
Size: 3040 112 772 4594
Fragments arranged by size:
4594 3040 772 112
Acc65I G'GTAC_C
Cuts at: 5839 5839
Size: 8518
AclI AA'CG_TT
Cuts at: 1563 6645 7018 1563
Size: 5082 373 3063
Fragments arranged by size:
5082 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3099 3575 4323 6581 7965 8233 1632
Size: 1467 476 748 2258 1384 268 1917
Fragments arranged by size:
2258 1917 1467 1384 748 476 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8321
AflIII A'CryG_T
Cuts at: 1203 2128 7417 8394 1203
Size: 925 5289 977 1327
Fragments arranged by size:
5289 1327 977 925
AgeI A'CCGG_T
Cuts at: 5052 5326 7349 5052
Size: 274 2023 6221
Fragments arranged by size:
6221 2023 274
AhdI GACnn_n'nnGTC
Cuts at: 7247 7247
Size: 8518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8518
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 8486
AlwNI CAG_nnn'CTG
Cuts at: 2885 3824 4301 5768 5807 6261 7833 2885
Size: 939 477 1467 39 454 1572 3570
Fragments arranged by size:
3570 1572 1467 939 477 454 39
ApaLI G'TGCA_C
Cuts at: 6575 7731 6575
Size: 1156 7362
ApoI r'AATT_y
Cuts at: 1493 2296 2850 4437 4479 4839 5550 5827 6053
Size: 803 554 1587 42 360 711 277 226
Cuts at: 6053 6205 8314 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1587 803 711 554 360 277
226 152 42
AseI AT'TA_AT
Cuts at: 160 1032 2621 3522 7072 160
Size: 872 1589 901 3550 1606
Fragments arranged by size:
3550 1606 1589 901 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8518
AvaI C'yCGr_G
Cuts at: 1870 1943 3537 5583 5843 1870
Size: 73 1594 2046 260 4545
Fragments arranged by size:
4545 2046 1594 260 73
BamHI G'GATC_C
Cuts at: 5848 6215 5848
Size: 367 8151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3940 4804 5839 7294 618
Size: 325 217 627 2153 864 1035 1455 1842
Fragments arranged by size:
2153 1842 1455 1035 864 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3589 3857 5837 1391
Size: 2198 268 1980 4072
Fragments arranged by size:
4072 2198 1980 268
BbeI G_GCGC'C
Cuts at: 3944 3944
Size: 8518
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2024 2823 4972 5194 5470 928
Size: 971 125 799 2149 222 276 3976
Fragments arranged by size:
3976 2149 971 799 276 222 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6709 6743 1903
Size: 34 4772 34 3678
Fragments arranged by size:
4772 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4751 4998 6410 7620 4751
Size: 247 1412 1210 5649
Fragments arranged by size:
5649 1412 1210 247
BclI T'GATC_A
Cuts at: 1548 3775 1548
Size: 2227 6291
BfrBI ATG'CAT
Cuts at: 3578 3578
Size: 8518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5284 7129
Size: 107 122 71 631 605 337 3275 1845
Cuts at: 7129 136
Size: 1525
Fragments arranged by size:
3275 1845 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2583 3407 8513 2583
Size: 824 5106 2588
Fragments arranged by size:
5106 2588 824
BlpI GC'TnA_GC
Cuts at: 5673 5935 5673
Size: 262 8256
Bme1580I G_kGCm'C
Cuts at: 1790 6579 7735 1790
Size: 4789 1156 2573
Fragments arranged by size:
4789 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4578 6249 7207
Size: 416 53 83 399 24 3161 1671 958
Cuts at: 7207 442
Size: 1753
Fragments arranged by size:
3161 1753 1671 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8518
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2490 3876 5688 2490
Size: 1386 1812 5320
Fragments arranged by size:
5320 1812 1386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 2460 3846 4074 4902 7178 1239
Size: 1170 51 1386 228 828 2276 2579
Fragments arranged by size:
2579 2276 1386 1170 828 228 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 3181 3292 6643 7508 7806 2583
Size: 598 111 3351 865 298 3295
Fragments arranged by size:
3351 3295 865 598 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5244 7181 882
Size: 617 3745 1937 2219
Fragments arranged by size:
3745 2219 1937 617
BsaAI yAC'GTr
Cuts at: 493 1204 6309 493
Size: 711 5105 2702
Fragments arranged by size:
5105 2702 711
BsaBI GATnn'nnATC
Cuts at: 3696 8512 3696
Size: 4816 3702
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3941 6325 6707 7342
Size: 53 83 186 936 2408 2384 382 635
Cuts at: 7342 275
Size: 1451
Fragments arranged by size:
2408 2384 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4174 5052 5326 6951 7349 7623 7770 8181 4174
Size: 878 274 1625 398 274 147 411 4511
Fragments arranged by size:
4511 1625 878 411 398 274 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2740 2770 1178
Size: 30 165 30 1337 30 6926
Fragments arranged by size:
6926 1337 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2982 3170 3900 3993 5307 5565 1186
Size: 1796 188 730 93 1314 258 4139
Fragments arranged by size:
4139 1796 1314 730 258 188 93
BseYI C'CCAG_C
Cuts at: 2655 7721 8217 2655
Size: 5066 496 2956
Fragments arranged by size:
5066 2956 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4172 1163
Size: 573 2436 5509
Fragments arranged by size:
5509 2436 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3338 5968 6729 7757 1540
Size: 275 186 1337 2630 761 1028 2301
Fragments arranged by size:
2630 2301 1337 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3589 6579 6664 7735 3589
Size: 2990 85 1071 4372
Fragments arranged by size:
4372 2990 1071 85
BsmI GAATG_Cn'
Cuts at: 3028 6029 6122 3028
Size: 3001 93 5424
Fragments arranged by size:
5424 3001 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3827 3827
Size: 8518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3589 3857 5837 6579 6664 7735 1391
Size: 399 1799 268 1980 742 85 1071 2174
Fragments arranged by size:
2174 1980 1799 1071 742 399 268 85
BspEI T'CCGG_A
Cuts at: 8181 8181
Size: 8518
BspHI T'CATG_A
Cuts at: 6407 8137 6407
Size: 1730 6788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5873 844
Size: 5029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3306 4253 6405 1394
Size: 429 1483 947 2152 3507
Fragments arranged by size:
3507 2152 1483 947 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7013 7187 65
Size: 1183 5765 174 1396
Fragments arranged by size:
5765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3994 5052 5326 7162 7349 1700
Size: 110 2184 1058 274 1836 187 2869
Fragments arranged by size:
2869 2184 1836 1058 274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8518
BssSI C'ACGA_G
Cuts at: 6578 7590 6578
Size: 1012 7506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8518
BstBI TT'CG_AA
Cuts at: 2069 7322 2069
Size: 5253 3265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8518
BstYI r'GATC_y
Cuts at: 1972 2583 3407 3697 5134 5848 6215 6600 6617
Size: 611 824 290 1437 714 367 385 17
Cuts at: 6617 8069 8155 8167 8513 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1437 824 714 611 385 367
346 290 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2994 5640 2994
Size: 2646 5872
BtgI C'CryG_G
Cuts at: 513 1065 2004 2534 3719 4903 513
Size: 552 939 530 1185 1184 4128
Fragments arranged by size:
4128 1185 1184 939 552 530
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7779
Fragments arranged by size:
7779 683 56
BtsI GCAGTG_nn'
Cuts at: 2457 6034 6828 6848 2457
Size: 3577 794 20 4127
Fragments arranged by size:
4127 3577 794 20
ClaI AT'CG_AT
Cuts at: 1554 5389 1554
Size: 3835 4683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8483
DraI TTT'AAA
Cuts at: 3654 5822 3654
Size: 2168 6350
DraIII CAC_nnn'GTG
Cuts at: 3422 4586 4904 3422
Size: 1164 318 7036
Fragments arranged by size:
7036 1164 318
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7525 809
Size: 507 6209 1802
Fragments arranged by size:
6209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 2505 4493
Size: 54 1425 50 275 18 615 60 1988
Cuts at: 4493 5965 6854 8483 8
Size: 1472 889 1629 43
Fragments arranged by size:
1988 1629 1472 1425 889 615 275 60
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5965 1537
Size: 275 4153 4090
Fragments arranged by size:
4153 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 3492 3594 4893 5242 5747 6448 1353
Size: 1794 345 102 1299 349 505 701 3423
Fragments arranged by size:
3423 1794 1299 701 505 349 345 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2409 7105 7479 7625 387
Size: 1064 138 820 4696 374 146 1280
Fragments arranged by size:
4696 1280 1064 820 374 146 138
EcoICRI GAG'CTC
Cuts at: 3587 3587
Size: 8518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 2460 3099 3575 3846 4074 4323
Size: 393 777 51 639 476 271 228 249
Cuts at: 4323 4902 6581 7178 7965 8233 1239
Size: 579 1679 597 787 268 1524
Fragments arranged by size:
1679 1524 787 777 639 597 579 476
393 271 268 249 228 51
EcoNI CCTnn'n_nnAGG
Cuts at: 5473 5473
Size: 8518
EcoO109I rG'GnC_Cy
Cuts at: 2674 5147 2674
Size: 2473 6045
EcoRI G'AATT_C
Cuts at: 4437 4839 5550 5827 6205 4437
Size: 402 711 277 378 6750
Fragments arranged by size:
6750 711 402 378 277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4839 5427 4839
Size: 588 7930
FspI TGC'GCA
Cuts at: 7024 7024
Size: 8518
HaeII r_GCGC'y
Cuts at: 1678 3944 7665 1678
Size: 2266 3721 2531
Fragments arranged by size:
3721 2531 2266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2407 2439 2943 2975 5299
Size: 32 135 32 835 32 504 32 2324
Cuts at: 5299 5331 7159 7191 7233 7265 1373
Size: 32 1828 32 42 32 2626
Fragments arranged by size:
2626 2324 1828 835 504 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4937 5862 6108 669
Size: 988 281 2999 925 246 3079
Fragments arranged by size:
3079 2999 988 925 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8518
HpaI GTT'AAC
Cuts at: 6108 6108
Size: 8518
KasI G'GCGC_C
Cuts at: 3940 3940
Size: 8518
KpnI G_GTAC'C
Cuts at: 5843 5843
Size: 8518
MfeI C'AATT_G
Cuts at: 6117 6117
Size: 8518
MluI A'CGCG_T
Cuts at: 8394 8394
Size: 8518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4174 4307 7632 7816 1305
Size: 2869 133 3325 184 2007
Fragments arranged by size:
3325 2869 2007 184 133
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2447 10
Size: 54 1425 343 615 6081
Fragments arranged by size:
6081 1425 615 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 3524 6476 6835 6994 518
Size: 648 1296 1062 2952 359 159 2042
Fragments arranged by size:
2952 2042 1296 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2729 2735 2741 4262 4712 5934 6611 7759
Size: 1123 6 6 1521 450 1222 677 1148
Cuts at: 7759 8004 1606
Size: 245 2120
Fragments arranged by size:
2120 1521 1222 1148 1123 677 450 245
6 6
NarI GG'CG_CC
Cuts at: 3941 3941
Size: 8518
NcoI C'CATG_G
Cuts at: 513 1065 2004 3719 513
Size: 552 939 1715 5312
Fragments arranged by size:
5312 1715 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8518
NotI GC'GGCC_GC
Cuts at: 5965 5965
Size: 8518
NsiI A_TGCA'T
Cuts at: 3580 3580
Size: 8518
NspI r_CATG'y
Cuts at: 1132 1229 2132 5672 5876 7421 1132
Size: 97 903 3540 204 1545 2229
Fragments arranged by size:
3540 2229 1545 903 204 97
PasI CC'CwG_GG
Cuts at: 1348 2312 5280 5469 1348
Size: 964 2968 189 4397
Fragments arranged by size:
4397 2968 964 189
PciI A'CATG_T
Cuts at: 2128 7417 2128
Size: 5289 3229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4073 4712 4904 5168 1108
Size: 2965 639 192 264 4458
Fragments arranged by size:
4458 2965 639 264 192
PfoI T'CCnGG_A
Cuts at: 2476 4006 4684 4786 4918 4960 5149 2476
Size: 1530 678 102 132 42 189 5845
Fragments arranged by size:
5845 1530 678 189 132 102 42
PmeI GTTT'AAAC
Cuts at: 5822 5822
Size: 8518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6587 6619 8100 8132 6587
Size: 32 1481 32 6973
Fragments arranged by size:
6973 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5147 5147
Size: 8518
PshAI GACnn'nnGTC
Cuts at: 1613 3881 4782 1613
Size: 2268 901 5349
Fragments arranged by size:
5349 2268 901
PsiI TTA'TAA
Cuts at: 6088 6088
Size: 8518
PstI C_TGCA'G
Cuts at: 830 2673 2734 2740 5516 5870 830
Size: 1843 61 6 2776 354 3478
Fragments arranged by size:
3478 2776 1843 354 61 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8518
PvuII CAG'CTG
Cuts at: 2729 2735 2741 4262 4712 2729
Size: 6 6 1521 450 6535
Fragments arranged by size:
6535 1521 450 6 6
SacI G_AGCT'C
Cuts at: 3589 3589
Size: 8518
SalI G'TCGA_C
Cuts at: 1936 5860 1936
Size: 3924 4594
SapI GCTCTTCn'nnn_
Cuts at: 3594 4893 3594
Size: 1299 7219
SbfI CC_TGCA'GG
Cuts at: 5870 5870
Size: 8518
ScaI AGT'ACT
Cuts at: 2343 3433 6766 8478 2343
Size: 1090 3333 1712 2383
Fragments arranged by size:
3333 2383 1712 1090
SexAI A'CCwGG_T
Cuts at: 4072 4072
Size: 8518
SfcI C'TryA_G
Cuts at: 826 1045 2574 2669 2730 2736 3954 5064 5130
Size: 219 1529 95 61 6 1218 1110 66
Cuts at: 5130 5196 5512 5866 7001 7682 7873 826
Size: 66 316 354 1135 681 191 1471
Fragments arranged by size:
1529 1471 1218 1135 1110 681 354 316
219 191 95 66 66 61 6
SfoI GGC'GCC
Cuts at: 3942 3942
Size: 8518
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8518
SmaI CCC'GGG
Cuts at: 1872 3539 5845 1872
Size: 1667 2306 4545
Fragments arranged by size:
4545 2306 1667
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2598 3196 3307 6622 7523 7785
Size: 197 926 655 598 111 3315 901 262
Cuts at: 7785 820
Size: 1553
Fragments arranged by size:
3315 1553 926 901 655 598 262 197
111
SnaBI TAC'GTA
Cuts at: 493 6309 493
Size: 5816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8518
SphI G_CATG'C
Cuts at: 1132 5876 1132
Size: 4744 3774
SspI AAT'ATT
Cuts at: 5 52 4546 4566 6442 5
Size: 47 4494 20 1876 2081
Fragments arranged by size:
4494 2081 1876 47 20
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8518
StyI C'CwwG_G
Cuts at: 513 1065 2004 2518 3627 3719 5411 5957 513
Size: 552 939 514 1109 92 1692 546 3074
Fragments arranged by size:
3074 1692 1109 939 552 546 514 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2077 2275 4597 5364 6545 6562 6715
Size: 1255 497 198 2322 767 1181 17 153
Cuts at: 6715 6900 7345 325
Size: 185 445 1498
Fragments arranged by size:
2322 1498 1255 1181 767 497 445 198
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2341 3431 4657
Size: 274 80 33 51 1443 364 1090 1226
Cuts at: 4657 5692 6764 8476 96
Size: 1035 1072 1712 138
Fragments arranged by size:
1712 1443 1226 1090 1072 1035 364 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2472 2504 2740 2772 5551 5583 8478 8510 2472
Size: 32 236 32 2779 32 2895 32 2480
Fragments arranged by size:
2895 2779 2480 236 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8518
XbaI T'CTAG_A
Cuts at: 3269 5854 3269
Size: 2585 5933
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4340 5277 4340
Size: 937 7581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8518
XmaI C'CCGG_G
Cuts at: 1870 3537 5843 1870
Size: 1667 2306 4545
Fragments arranged by size:
4545 2306 1667
XmnI GAAnn'nnTTC
Cuts at: 2021 3211 6647 2021
Size: 1190 3436 3892
Fragments arranged by size:
3892 3436 1190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6326 276
Size: 53 83 186 936 4792 2468
Fragments arranged by size:
4792 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SrfI SwaI