Restriction map for
pFN21AA1114
(Circular) (Six-base) MAPSORT of: pFN21AA1114.seq Check: 5 from: 1 to: 8998
pFN21AA1114 8998 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2263 2263
Size: 8998
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6808 278
Size: 53 83 186 936 5272 2468
Fragments arranged by size:
5272 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6341 1937
Size: 4404 4594
Acc65I G'GTAC_C
Cuts at: 5735 6319 5735
Size: 584 8414
AclI AA'CG_TT
Cuts at: 1563 7125 7498 1563
Size: 5562 373 3063
Fragments arranged by size:
5562 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2379 2522 3996 4156 4186 4240 4294 4348
Size: 747 143 1474 160 30 54 54 54
Cuts at: 4348 4624 5224 5254 5674 5794 6058 6088 6145
Size: 276 600 30 420 120 264 30 57
Cuts at: 6145 7061 8445 8713 1632
Size: 916 1384 268 1917
Fragments arranged by size:
1917 1474 1384 916 747 600 420 276
268 264 160 143 120 57 54 54
54 30 30 30
AfeI AGC'GCT
Cuts at: 5743 5743
Size: 8998
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8801
AflIII A'CryG_T
Cuts at: 1203 7897 8874 1203
Size: 6694 977 1327
Fragments arranged by size:
6694 1327 977
AgeI A'CCGG_T
Cuts at: 5588 7829 5588
Size: 2241 6757
AhdI GACnn_n'nnGTC
Cuts at: 7727 7727
Size: 8998
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8998
AlwNI CAG_nnn'CTG
Cuts at: 2402 3169 4695 4755 4875 5025 5145 5445 5475
Size: 767 1526 60 120 150 120 300 30
Cuts at: 5475 5535 6185 6741 8313 2402
Size: 60 650 556 1572 3087
Fragments arranged by size:
3087 1572 1526 767 650 556 300 150
120 120 60 60 30
ApaI G_GGCC'C
Cuts at: 2054 2933 3097 4035 2054
Size: 879 164 938 7017
Fragments arranged by size:
7017 938 879 164
ApaLI G'TGCA_C
Cuts at: 4233 4395 4617 5067 5187 5277 7055 8211 4233
Size: 162 222 450 120 90 1778 1156 5020
Fragments arranged by size:
5020 1778 1156 450 222 162 120 90
ApoI r'AATT_y
Cuts at: 1493 2935 6307 6533 6685 8794 1493
Size: 1442 3372 226 152 2109 1697
Fragments arranged by size:
3372 2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7552 160
Size: 872 6520 1606
Fragments arranged by size:
6520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8998
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 3054 3219 6323 1870
Size: 73 122 989 165 3104 4545
Fragments arranged by size:
4545 3104 989 165 122 73
AvrII C'CTAG_G
Cuts at: 5671 5671
Size: 8998
BamHI G'GATC_C
Cuts at: 3288 6328 6695 3288
Size: 3040 367 5591
Fragments arranged by size:
5591 3040 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 5045 5435 5675 5735
Size: 325 217 627 248 3010 390 240 60
Cuts at: 5735 5765 5795 5825 5885 5915 6275 6319 7774
Size: 30 30 30 60 30 360 44 1455
Cuts at: 7774 618
Size: 1842
Fragments arranged by size:
3010 1842 1455 627 390 360 325 248
240 217 60 60 44 30 30 30
30
BanII G_rGCy'C
Cuts at: 1391 2054 2933 3034 3097 3207 4000 4035 4138
Size: 663 879 101 63 110 793 35 103
Cuts at: 4138 5461 6317 1391
Size: 1323 856 4072
Fragments arranged by size:
4072 1323 879 856 793 663 110 103
101 63 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2795 4630 928
Size: 971 896 1835 5296
Fragments arranged by size:
5296 1835 971 896
BbvCI CC'TCA_GC
Cuts at: 3416 3969 3987 5942 3416
Size: 553 18 1955 6472
Fragments arranged by size:
6472 1955 553 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7189 7223 1903
Size: 34 5252 34 3678
Fragments arranged by size:
5252 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2747 6890 8100 2747
Size: 4143 1210 3645
Fragments arranged by size:
4143 3645 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8998
BfrBI ATG'CAT
Cuts at: 2140 2140
Size: 8998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3050 3659 7609
Size: 107 122 71 631 605 1378 609 3950
Cuts at: 7609 136
Size: 1525
Fragments arranged by size:
3950 1525 1378 631 609 605 122 107
71
BglII A'GATC_T
Cuts at: 2949 8993 2949
Size: 6044 2954
BlpI GC'TnA_GC
Cuts at: 2406 4652 6415 2406
Size: 2246 1763 4989
Fragments arranged by size:
4989 2246 1763
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2933 3097 3136 4035 4237 4399 4621
Size: 264 879 164 39 899 202 162 222
Cuts at: 4621 5071 5191 5281 5341 5798 5828 5888 7059
Size: 450 120 90 60 457 30 60 1171
Cuts at: 7059 8215 1790
Size: 1156 2573
Fragments arranged by size:
2573 1171 1156 899 879 457 450 264
222 202 164 162 120 90 60 60
39 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3584 3916 5801
Size: 416 53 83 399 24 2167 332 1885
Cuts at: 5801 5831 5891 6081 6729 7687 442
Size: 30 60 190 648 958 1753
Fragments arranged by size:
2167 1885 1753 958 648 416 399 332
190 83 60 53 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4697 4577
Size: 120 8878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 2250 3221 3503 3794 4123 4228 4342
Size: 837 174 971 282 291 329 105 114
Cuts at: 4342 4702 7658 1239
Size: 360 2956 2579
Fragments arranged by size:
2956 2579 971 837 360 329 291 282
174 114 105
Bpu10I CC'TnA_GC
Cuts at: 2799 3416 3969 3987 4095 5702 5942 2799
Size: 617 553 18 108 1607 240 5855
Fragments arranged by size:
5855 1607 617 553 240 108 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3158 3851 7123 7988 8286 3158
Size: 693 3272 865 298 3870
Fragments arranged by size:
3870 3272 865 693 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 2674 3611 7661 882
Size: 617 603 572 937 4050 2219
Fragments arranged by size:
4050 2219 937 617 603 572
BsaAI yAC'GTr
Cuts at: 493 1204 6789 493
Size: 711 5585 2702
Fragments arranged by size:
5585 2702 711
BsaBI GATnn'nnATC
Cuts at: 3180 3395 8992 3180
Size: 215 5597 3186
Fragments arranged by size:
5597 3186 215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6805 7187 7822 275
Size: 53 83 186 936 5272 382 635 1451
Fragments arranged by size:
5272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5588 7431 7829 8103 8250 8661 5588
Size: 1843 398 274 147 411 5925
Fragments arranged by size:
5925 1843 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3476 3506 1178
Size: 30 165 30 2073 30 6670
Fragments arranged by size:
6670 2073 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3599 4194 1186
Size: 2413 595 5990
Fragments arranged by size:
5990 2413 595
BseYI C'CCAG_C
Cuts at: 2272 3601 4028 4238 4292 4712 4892 5162 5372
Size: 1329 427 210 54 420 180 270 210
Cuts at: 5372 5402 5462 5576 6056 8201 8697 2272
Size: 30 60 114 480 2145 496 2573
Fragments arranged by size:
2573 2145 1329 496 480 427 420 270
210 210 180 114 60 54 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2235 3294 3905 3935 4759 1163
Size: 573 499 1059 611 30 824 5402
Fragments arranged by size:
5402 1059 824 611 573 499 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6448 7209 8237 1540
Size: 275 186 4447 761 1028 2301
Fragments arranged by size:
4447 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4237 4352 4399 4621 4634 4921 4953 4988 5071
Size: 115 47 222 13 287 32 35 83
Cuts at: 5071 5191 5281 5431 5461 6179 6239 7059 7144
Size: 120 90 150 30 718 60 820 85
Cuts at: 7144 8215 4237
Size: 1071 5020
Fragments arranged by size:
5020 1071 820 718 287 222 150 120
115 90 85 83 60 47 35 32
30 13
BsmI GAATG_Cn'
Cuts at: 3712 6509 6602 3712
Size: 2797 93 6108
Fragments arranged by size:
6108 2797 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2933 3034 3097 3136 3207 4000
Size: 399 264 879 101 63 39 71 793
Cuts at: 4000 4035 4138 4237 4352 4399 4621 4634 4921
Size: 35 103 99 115 47 222 13 287
Cuts at: 4921 4953 4988 5071 5191 5281 5341 5431 5461
Size: 32 35 83 120 90 60 90 30
Cuts at: 5461 5798 5828 5888 6179 6239 6317 7059 7144
Size: 337 30 60 291 60 78 742 85
Cuts at: 7144 8215 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 879 793 742 399 337 291
287 264 222 120 115 103 101 99
90 90 85 83 78 71 63 60
60 60 47 39 35 35 32 30
30 13
BspEI T'CCGG_A
Cuts at: 8661 8661
Size: 8998
BspHI T'CATG_A
Cuts at: 6887 8617 6887
Size: 1730 7268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2263 2337 2506 6353 844
Size: 1419 74 169 3847 3489
Fragments arranged by size:
3847 3489 1419 169 74
BsrBI CCG'CTC
Cuts at: 1394 1823 6885 1394
Size: 429 5062 3507
Fragments arranged by size:
5062 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5611 7493 7667 65
Size: 1183 4363 1882 174 1396
Fragments arranged by size:
4363 1882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 5588 7642 7829 1700
Size: 110 386 3392 2054 187 2869
Fragments arranged by size:
3392 2869 2054 386 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8998
BssSI C'ACGA_G
Cuts at: 3842 7058 8070 3842
Size: 3216 1012 4770
Fragments arranged by size:
4770 3216 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2258 794
Size: 1464 7534
BstBI TT'CG_AA
Cuts at: 7802 7802
Size: 8998
BstEII G'GTnAC_C
Cuts at: 1184 2374 1184
Size: 1190 7808
BstXI CCAn_nnnn'nTGG
Cuts at: 2486 2486
Size: 8998
BstYI r'GATC_y
Cuts at: 1972 2949 3288 3390 6328 6695 7080 7097 8549
Size: 977 339 102 2938 367 385 17 1452
Cuts at: 8549 8635 8647 8993 1972
Size: 86 12 346 1977
Fragments arranged by size:
2938 1977 1452 977 385 367 346 339
102 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2175 3293 4569 513
Size: 552 939 171 1118 1276 4942
Fragments arranged by size:
4942 1276 1118 939 552 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4101 4221 5967 487
Size: 683 56 2875 120 1746 3518
Fragments arranged by size:
3518 2875 1746 683 120 56
BtsI GCAGTG_nn'
Cuts at: 2400 3936 4429 4621 4951 5071 5491 6025 6145
Size: 1536 493 192 330 120 420 534 120
Cuts at: 6145 6262 6514 7308 7328 2400
Size: 117 252 794 20 4070
Fragments arranged by size:
4070 1536 794 534 493 420 330 252
192 120 120 117 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4834 4869 529
Size: 35 4270 35 4658
Fragments arranged by size:
4658 4270 35 35
DraI TTT'AAA
Cuts at: 2325 6302 2325
Size: 3977 5021
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8005 809
Size: 507 6689 1802
Fragments arranged by size:
6689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2476 3327
Size: 54 1425 50 275 18 342 304 851
Cuts at: 3327 6445 7334 8963 8
Size: 3118 889 1629 43
Fragments arranged by size:
3118 1629 1425 889 851 342 304 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6445 1537
Size: 275 4633 4090
Fragments arranged by size:
4633 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2426 4071 4834 6928 1353
Size: 759 314 1645 763 2094 3423
Fragments arranged by size:
3423 2094 1645 763 759 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3301 5832 7585 7959 8105 387
Size: 1064 138 1712 2531 1753 374 146 1280
Fragments arranged by size:
2531 1753 1712 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5459 5459
Size: 8998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2250 2379 2522 3221 3503 3794
Size: 393 444 174 129 143 699 282 291
Cuts at: 3794 3996 4123 4156 4186 4228 4240 4294 4342
Size: 202 127 33 30 42 12 54 48
Cuts at: 4342 4348 4624 4702 5224 5254 5674 5794 6058
Size: 6 276 78 522 30 420 120 264
Cuts at: 6058 6088 6145 7061 7658 8445 8713 1239
Size: 30 57 916 597 787 268 1524
Fragments arranged by size:
1524 916 787 699 597 522 444 420
393 291 282 276 268 264 202 174
143 129 127 120 78 57 54 48
42 33 30 30 30 12 6
EcoO109I rG'GnC_Cy
Cuts at: 2050 2051 2224 2518 2929 2959 3093 3166 3216
Size: 1 173 294 411 30 134 73 50
Cuts at: 3216 3787 4032 2050
Size: 571 245 7016
Fragments arranged by size:
7016 571 411 294 245 173 134 73
50 30 1
EcoRI G'AATT_C
Cuts at: 6307 6685 6307
Size: 378 8620
EcoRV GAT'ATC
Cuts at: 4057 4057
Size: 8998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 8998
FspI TGC'GCA
Cuts at: 3683 7504 3683
Size: 3821 5177
HaeII r_GCGC'y
Cuts at: 1678 5745 8145 1678
Size: 4067 2400 2531
Fragments arranged by size:
4067 2531 2400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 7639 7671 7713 7745 1373
Size: 32 135 32 6067 32 42 32 2626
Fragments arranged by size:
6067 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2194 4168 6342 6588 669
Size: 988 281 256 1974 2174 246 3079
Fragments arranged by size:
3079 2174 1974 988 281 256 246
HindIII A'AGCT_T
Cuts at: 748 2616 748
Size: 1868 7130
HpaI GTT'AAC
Cuts at: 6588 6588
Size: 8998
KpnI G_GTAC'C
Cuts at: 5739 6323 5739
Size: 584 8414
MfeI C'AATT_G
Cuts at: 2559 2793 6597 2559
Size: 234 3804 4960
Fragments arranged by size:
4960 3804 234
MluI A'CGCG_T
Cuts at: 8874 8874
Size: 8998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6051 8112 8296 1305
Size: 4746 2061 184 2007
Fragments arranged by size:
4746 2061 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2478 3329 10
Size: 54 1425 343 342 304 851 5679
Fragments arranged by size:
5679 1425 851 343 342 304 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2484 3252 3635 6956 7315 7474 518
Size: 648 1318 768 383 3321 359 159 2042
Fragments arranged by size:
3321 2042 1318 768 648 383 359 159
MspA1I CmG'CkG
Cuts at: 1606 2276 2500 3956 5351 6108 6165 6414 7091
Size: 670 224 1456 1395 757 57 249 677
Cuts at: 7091 8239 8484 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1456 1395 1148 757 677 670 249
245 224 57
NcoI C'CATG_G
Cuts at: 513 1065 2004 2175 3293 513
Size: 552 939 171 1118 6218
Fragments arranged by size:
6218 1118 939 552 171
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8998
NotI GC'GGCC_GC
Cuts at: 6445 6445
Size: 8998
NsiI A_TGCA'T
Cuts at: 2142 2142
Size: 8998
NspI r_CATG'y
Cuts at: 1132 1229 3415 3879 4453 6356 7901 1132
Size: 97 2186 464 574 1903 1545 2229
Fragments arranged by size:
2229 2186 1903 1545 574 464 97
PasI CC'CwG_GG
Cuts at: 1348 3701 1348
Size: 2353 6645
PciI A'CATG_T
Cuts at: 7897 7897
Size: 8998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2995 3169 3906 6122 6209 1108
Size: 1887 174 737 2216 87 3897
Fragments arranged by size:
3897 2216 1887 737 174 87
PmeI GTTT'AAAC
Cuts at: 6302 6302
Size: 8998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7067 7099 8580 8612 7067
Size: 32 1481 32 7453
Fragments arranged by size:
7453 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 3216 3787 2518
Size: 698 571 7729
Fragments arranged by size:
7729 698 571
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8998
PsiI TTA'TAA
Cuts at: 6568 6568
Size: 8998
PspOMI G'GGCC_C
Cuts at: 2050 2929 3093 4031 2050
Size: 879 164 938 7017
Fragments arranged by size:
7017 938 879 164
PstI C_TGCA'G
Cuts at: 830 3568 3955 6140 6350 830
Size: 2738 387 2185 210 3478
Fragments arranged by size:
3478 2738 2185 387 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8998
PvuII CAG'CTG
Cuts at: 2276 2500 3956 2276
Size: 224 1456 7318
Fragments arranged by size:
7318 1456 224
SacI G_AGCT'C
Cuts at: 5461 5461
Size: 8998
SalI G'TCGA_C
Cuts at: 1936 6340 1936
Size: 4404 4594
SanDI GG'GwC_CC
Cuts at: 3787 3787
Size: 8998
SapI GCTCTTCn'nnn_
Cuts at: 4834 4834
Size: 8998
SbfI CC_TGCA'GG
Cuts at: 6350 6350
Size: 8998
ScaI AGT'ACT
Cuts at: 4963 7246 8958 4963
Size: 2283 1712 5003
Fragments arranged by size:
5003 2283 1712
SfcI C'TryA_G
Cuts at: 826 1045 3564 3951 6136 6346 7481 8162 8353
Size: 219 2519 387 2185 210 1135 681 191
Cuts at: 8353 826
Size: 1471
Fragments arranged by size:
2519 2185 1471 1135 681 387 219 210
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8998
SmaI CCC'GGG
Cuts at: 1872 2067 3221 6325 1872
Size: 195 1154 3104 4545
Fragments arranged by size:
4545 3104 1154 195
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3137 3866 7102 8003 8265 820
Size: 197 926 1194 729 3236 901 262 1553
Fragments arranged by size:
3236 1553 1194 926 901 729 262 197
SnaBI TAC'GTA
Cuts at: 493 6789 493
Size: 6296 2702
SpeI A'CTAG_T
Cuts at: 152 4412 5858 152
Size: 4260 1446 3292
Fragments arranged by size:
4260 3292 1446
SphI G_CATG'C
Cuts at: 1132 3879 4453 6356 1132
Size: 2747 574 1903 3774
Fragments arranged by size:
3774 2747 1903 574
SspI AAT'ATT
Cuts at: 5 52 2548 3446 6922 5
Size: 47 2496 898 3476 2081
Fragments arranged by size:
3476 2496 2081 898 47
StuI AGG'CCT
Cuts at: 1517 2873 3307 4100 5971 1517
Size: 1356 434 793 1871 4544
Fragments arranged by size:
4544 1871 1356 793 434
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 2175 2445 2455 2622 2707
Size: 552 939 156 15 270 10 167 85
Cuts at: 2707 2904 3088 3293 3585 3900 5671 6437 513
Size: 197 184 205 292 315 1771 766 3074
Fragments arranged by size:
3074 1771 939 766 552 315 292 270
205 197 184 167 156 85 15 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2705 2720 3610 4252 4306 4636 6187
Size: 1255 1125 15 890 642 54 330 1551
Cuts at: 6187 6247 7025 7042 7195 7380 7825 325
Size: 60 778 17 153 185 445 1498
Fragments arranged by size:
1551 1498 1255 1125 890 778 642 445
330 185 153 60 54 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3777 4588 4961
Size: 274 80 33 51 1443 1800 811 373
Cuts at: 4961 7244 8956 96
Size: 2283 1712 138
Fragments arranged by size:
2283 1800 1712 1443 811 373 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 8958 8990 2019
Size: 32 6907 32 2027
Fragments arranged by size:
6907 2027 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2126 1316
Size: 810 8188
XbaI T'CTAG_A
Cuts at: 6334 6334
Size: 8998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2471 4007 2167
Size: 304 1536 7158
Fragments arranged by size:
7158 1536 304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8998
XmaI C'CCGG_G
Cuts at: 1870 2065 3219 6323 1870
Size: 195 1154 3104 4545
Fragments arranged by size:
4545 3104 1154 195
XmnI GAAnn'nnTTC
Cuts at: 7127 7127
Size: 8998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6806 276
Size: 53 83 186 936 5272 2468
Fragments arranged by size:
5272 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI