Restriction map for
pFN21AA1113
(Circular) (Six-base) MAPSORT of: pFN21AA1113.seq Check: 6896 from: 1 to: 8086
pFN21AA1113 8086 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2384 3217 2384
Size: 833 7253
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5896 278
Size: 53 83 186 936 4360 2468
Fragments arranged by size:
4360 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5429 1937
Size: 3492 4594
Acc65I G'GTAC_C
Cuts at: 5407 5407
Size: 8086
AclI AA'CG_TT
Cuts at: 1563 6213 6586 1563
Size: 4650 373 3063
Fragments arranged by size:
4650 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2634 4368 5018 6149 7533 7801 1632
Size: 1002 1734 650 1131 1384 268 1917
Fragments arranged by size:
1917 1734 1384 1131 1002 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7889
AflIII A'CryG_T
Cuts at: 1203 2723 2873 2888 6985 7962 1203
Size: 1520 150 15 4097 977 1327
Fragments arranged by size:
4097 1520 1327 977 150 15
AgeI A'CCGG_T
Cuts at: 6917 6917
Size: 8086
AhdI GACnn_n'nnGTC
Cuts at: 6815 6815
Size: 8086
AleI CACnn'nnGTG
Cuts at: 1166 2123 4220 1166
Size: 957 2097 5032
Fragments arranged by size:
5032 2097 957
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8086
AlwNI CAG_nnn'CTG
Cuts at: 2798 3455 3629 5829 7401 2798
Size: 657 174 2200 1572 3483
Fragments arranged by size:
3483 2200 1572 657 174
ApaI G_GGCC'C
Cuts at: 2091 2190 2091
Size: 99 7987
ApaLI G'TGCA_C
Cuts at: 6143 7299 6143
Size: 1156 6930
ApoI r'AATT_y
Cuts at: 1493 2750 4946 5395 5621 5773 7882 1493
Size: 1257 2196 449 226 152 2109 1697
Fragments arranged by size:
2196 2109 1697 1257 449 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3123 5166 6640 160
Size: 872 2091 2043 1474 1606
Fragments arranged by size:
2091 2043 1606 1474 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8086
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 2468 2500 5411 1870
Size: 73 275 250 32 2911 4545
Fragments arranged by size:
4545 2911 275 250 73 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2912 2945 2912
Size: 33 8053
BamHI G'GATC_C
Cuts at: 5416 5783 5416
Size: 367 7719
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2544 5407 6862 618
Size: 325 217 627 757 2863 1455 1842
Fragments arranged by size:
2863 1842 1455 757 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2190 2225 2424 3938 4566 5405 1391
Size: 700 99 35 199 1514 628 839 4072
Fragments arranged by size:
4072 1514 839 700 628 199 99 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2432 2673 4298 4671 4901 5331 928
Size: 971 533 241 1625 373 230 430 3683
Fragments arranged by size:
3683 1625 971 533 430 373 241 230
BbvCI CC'TCA_GC
Cuts at: 2169 2169
Size: 8086
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6277 6311 1903
Size: 34 4340 34 3678
Fragments arranged by size:
4340 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3801 5978 7188 3801
Size: 2177 1210 4699
Fragments arranged by size:
4699 2177 1210
BclI T'GATC_A
Cuts at: 1548 2769 3711 4315 1548
Size: 1221 942 604 5319
Fragments arranged by size:
5319 1221 942 604
BfrBI ATG'CAT
Cuts at: 4493 4493
Size: 8086
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6697 136
Size: 107 122 71 631 605 5025 1525
Fragments arranged by size:
5025 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4696 5241 8081 4696
Size: 545 2840 4701
Fragments arranged by size:
4701 2840 545
BlpI GC'TnA_GC
Cuts at: 2486 4565 5503 2486
Size: 2079 938 5069
Fragments arranged by size:
5069 2079 938
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2190 2264 2496 2549 6147 7303 1790
Size: 301 99 74 232 53 3598 1156 2573
Fragments arranged by size:
3598 2573 1156 301 232 99 74 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2543 5817 6775
Size: 416 53 83 399 24 1126 3274 958
Cuts at: 6775 442
Size: 1753
Fragments arranged by size:
3274 1753 1126 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8086
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3798 4715 5263 3798
Size: 917 548 6621
Fragments arranged by size:
6621 917 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2286 2289 3477 3536 4149 6746 1239
Size: 1044 3 3 1188 59 613 2597 2579
Fragments arranged by size:
2597 2579 1188 1044 613 59 3 3
Bpu10I CC'TnA_GC
Cuts at: 2169 5193 2169
Size: 3024 5062
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3017 4066 6211 7076 7374 3017
Size: 1049 2145 865 298 3729
Fragments arranged by size:
3729 2145 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4219 5362 6749 882
Size: 617 2720 1143 1387 2219
Fragments arranged by size:
2720 2219 1387 1143 617
BsaAI yAC'GTr
Cuts at: 493 1204 5877 493
Size: 711 4673 2702
Fragments arranged by size:
4673 2702 711
BsaBI GATnn'nnATC
Cuts at: 8080 8080
Size: 8086
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2291 5893 6275 6910
Size: 53 83 186 936 758 3602 382 635
Cuts at: 6910 275
Size: 1451
Fragments arranged by size:
3602 1451 936 758 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6519 6917 7191 7338 7749 6519
Size: 398 274 147 411 6856
Fragments arranged by size:
6856 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2281 2311 1178
Size: 30 165 30 878 30 6953
Fragments arranged by size:
6953 878 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2153 2156 2159 2206 3693 4423 4644
Size: 964 3 3 3 47 1487 730 221
Cuts at: 4644 4707 1186
Size: 63 4565
Fragments arranged by size:
4565 1487 964 730 221 63 47 3
3 3
BseYI C'CCAG_C
Cuts at: 7289 7785 7289
Size: 496 7590
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2700 3056 1163
Size: 573 964 356 6193
Fragments arranged by size:
6193 964 573 356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2184 5536 6297 7325 1540
Size: 275 186 183 3352 761 1028 2301
Fragments arranged by size:
3352 2301 1028 761 275 186 183
BsiHKAI G_wGCw'C
Cuts at: 6147 6232 7303 6147
Size: 85 1071 6930
Fragments arranged by size:
6930 1071 85
BsmI GAATG_Cn'
Cuts at: 2570 3793 5597 5690 2570
Size: 1223 1804 93 4966
Fragments arranged by size:
4966 1804 1223 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2190 2225 2264 2424 2496 2549
Size: 399 301 99 35 39 160 72 53
Cuts at: 2549 3938 4566 5405 6147 6232 7303 1391
Size: 1389 628 839 742 85 1071 2174
Fragments arranged by size:
2174 1389 1071 839 742 628 399 301
160 99 85 72 53 39 35
BspEI T'CCGG_A
Cuts at: 7749 7749
Size: 8086
BspHI T'CATG_A
Cuts at: 5975 7705 5975
Size: 1730 6356
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2384 3217 4476 4537 5441 844
Size: 1540 833 1259 61 904 3489
Fragments arranged by size:
3489 1540 1259 904 833 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 2321 5973 1394
Size: 429 217 281 3652 3507
Fragments arranged by size:
3652 3507 429 281 217
BsrDI GCAATG_nn'
Cuts at: 65 1248 6581 6755 65
Size: 1183 5333 174 1396
Fragments arranged by size:
5333 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2162 2293 2406 6730 6917 1700
Size: 110 255 97 131 113 4324 187 2869
Fragments arranged by size:
4324 2869 255 187 131 113 110 97
BsrGI T'GTAC_A
Cuts at: 96 2836 96
Size: 2740 5346
BssHII G'CGCG_C
Cuts at: 1925 2061 1925
Size: 136 7950
BssSI C'ACGA_G
Cuts at: 6146 7158 6146
Size: 1012 7074
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2398 3727 794
Size: 1604 1329 5153
Fragments arranged by size:
5153 1604 1329
BstBI TT'CG_AA
Cuts at: 4597 6890 4597
Size: 2293 5793
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8086
BstXI CCAn_nnnn'nTGG
Cuts at: 2531 4066 2531
Size: 1535 6551
BstYI r'GATC_y
Cuts at: 1972 4696 5241 5416 5783 6168 6185 7637 7723
Size: 2724 545 175 367 385 17 1452 86
Cuts at: 7723 7735 8081 1972
Size: 12 346 1977
Fragments arranged by size:
2724 1977 1452 545 385 367 346 175
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2208 4884 513
Size: 552 939 117 87 2676 3715
Fragments arranged by size:
3715 2676 939 552 117 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4023 487
Size: 683 56 2797 4550
Fragments arranged by size:
4550 2797 683 56
BtsI GCAGTG_nn'
Cuts at: 5602 6396 6416 5602
Size: 794 20 7272
Fragments arranged by size:
7272 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8051
DraI TTT'AAA
Cuts at: 5390 5390
Size: 8086
DraIII CAC_nnn'GTG
Cuts at: 3344 3344
Size: 8086
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7093 809
Size: 507 5777 1802
Fragments arranged by size:
5777 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2205 3096
Size: 54 1425 50 275 18 351 24 891
Cuts at: 3096 5079 5533 6422 8051 8
Size: 1983 454 889 1629 43
Fragments arranged by size:
1983 1629 1425 891 889 454 351 275
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2181 5533 1537
Size: 275 369 3352 4090
Fragments arranged by size:
4090 3352 369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5283 6016 1353
Size: 3930 733 3423
Fragments arranged by size:
3930 3423 733
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2120 2432 6673 7047 7193
Size: 1064 138 435 96 312 4241 374 146
Cuts at: 7193 387
Size: 1280
Fragments arranged by size:
4241 1280 1064 435 374 312 146 138
96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2286 2289 2634 3477 3536 4149
Size: 393 651 3 3 345 843 59 613
Cuts at: 4149 4368 5018 6149 6746 7533 7801 1239
Size: 219 650 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 843 787 651 650 613 597
393 345 268 219 59 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 4164 4164
Size: 8086
EcoO109I rG'GnC_Cy
Cuts at: 2327 3971 4169 5256 2327
Size: 1644 198 1087 5157
Fragments arranged by size:
5157 1644 1087 198
EcoRI G'AATT_C
Cuts at: 4946 5395 5773 4946
Size: 449 378 7259
Fragments arranged by size:
7259 449 378
FspI TGC'GCA
Cuts at: 4869 6592 4869
Size: 1723 6363
HaeII r_GCGC'y
Cuts at: 1678 2169 2482 7233 1678
Size: 491 313 4751 2531
Fragments arranged by size:
4751 2531 491 313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 2624 2656 4064
Size: 32 135 32 709 32 311 32 1408
Cuts at: 4064 4096 4438 4470 6727 6759 6801 6833 1373
Size: 32 342 32 2257 32 42 32 2626
Fragments arranged by size:
2626 2257 1408 709 342 311 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2816 5430 5676 669
Size: 988 281 878 2614 246 3079
Fragments arranged by size:
3079 2614 988 878 281 246
HindIII A'AGCT_T
Cuts at: 748 2946 5030 748
Size: 2198 2084 3804
Fragments arranged by size:
3804 2198 2084
HpaI GTT'AAC
Cuts at: 5676 5676
Size: 8086
KpnI G_GTAC'C
Cuts at: 5411 5411
Size: 8086
MfeI C'AATT_G
Cuts at: 2970 3261 5685 2970
Size: 291 2424 5371
Fragments arranged by size:
5371 2424 291
MluI A'CGCG_T
Cuts at: 7962 7962
Size: 8086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2782 4047 4775 7200 7384 1305
Size: 1477 1265 728 2425 184 2007
Fragments arranged by size:
2425 2007 1477 1265 728 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3098 10
Size: 54 1425 343 1266 4998
Fragments arranged by size:
4998 1425 1266 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 4220 6044 6403 6562 518
Size: 648 957 2097 1824 359 159 2042
Fragments arranged by size:
2097 2042 1824 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2123 2210 3822 4263 5502 6179 7327
Size: 449 68 87 1612 441 1239 677 1148
Cuts at: 7327 7572 1606
Size: 245 2120
Fragments arranged by size:
2120 1612 1239 1148 677 449 441 245
87 68
NaeI GCC'GGC
Cuts at: 2164 2295 2408 2164
Size: 131 113 7842
Fragments arranged by size:
7842 131 113
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6595
Fragments arranged by size:
6595 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8086
NgoMIV G'CCGG_C
Cuts at: 2162 2293 2406 2162
Size: 131 113 7842
Fragments arranged by size:
7842 131 113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8086
NotI GC'GGCC_GC
Cuts at: 5533 5533
Size: 8086
NsiI A_TGCA'T
Cuts at: 4495 4495
Size: 8086
NspI r_CATG'y
Cuts at: 1132 1229 2727 2877 2892 3239 3562 3884 4423
Size: 97 1498 150 15 347 323 322 539
Cuts at: 4423 5444 6989 1132
Size: 1021 1545 2229
Fragments arranged by size:
2229 1545 1498 1021 539 347 323 322
150 97 15
PasI CC'CwG_GG
Cuts at: 1348 3875 1348
Size: 2527 5559
PciI A'CATG_T
Cuts at: 2723 2873 2888 6985 2723
Size: 150 15 4097 3824
Fragments arranged by size:
4097 3824 150 15
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3399 3455 1108
Size: 2291 56 5739
Fragments arranged by size:
5739 2291 56
PfoI T'CCnGG_A
Cuts at: 2347 2367 4186 4950 5064 2347
Size: 20 1819 764 114 5369
Fragments arranged by size:
5369 1819 764 114 20
PmeI GTTT'AAAC
Cuts at: 5390 5390
Size: 8086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6155 6187 7668 7700 6155
Size: 32 1481 32 6541
Fragments arranged by size:
6541 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2327 3971 4169 5256 2327
Size: 1644 198 1087 5157
Fragments arranged by size:
5157 1644 1087 198
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8086
PsiI TTA'TAA
Cuts at: 4994 5656 4994
Size: 662 7424
PspOMI G'GGCC_C
Cuts at: 2087 2186 2087
Size: 99 7987
PstI C_TGCA'G
Cuts at: 830 4534 5438 830
Size: 3704 904 3478
Fragments arranged by size:
3704 3478 904
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8086
PvuII CAG'CTG
Cuts at: 4263 4263
Size: 8086
SacII CC_GC'GG
Cuts at: 2124 2211 2124
Size: 87 7999
SalI G'TCGA_C
Cuts at: 1936 5428 1936
Size: 3492 4594
SanDI GG'GwC_CC
Cuts at: 2327 2327
Size: 8086
SbfI CC_TGCA'GG
Cuts at: 5438 5438
Size: 8086
ScaI AGT'ACT
Cuts at: 4237 4246 6334 8046 4237
Size: 9 2088 1712 4277
Fragments arranged by size:
4277 2088 1712 9
SfcI C'TryA_G
Cuts at: 826 1045 3191 3942 4530 5434 6569 7250 7441
Size: 219 2146 751 588 904 1135 681 191
Cuts at: 7441 826
Size: 1471
Fragments arranged by size:
2146 1471 1135 904 751 681 588 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8086
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8086
SmaI CCC'GGG
Cuts at: 1872 2502 5413 1872
Size: 630 2911 4545
Fragments arranged by size:
4545 2911 630
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2996 4081 6190 7091 7353 820
Size: 197 926 1053 1085 2109 901 262 1553
Fragments arranged by size:
2109 1553 1085 1053 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5877 493
Size: 5384 2702
SpeI A'CTAG_T
Cuts at: 152 2657 152
Size: 2505 5581
SphI G_CATG'C
Cuts at: 1132 3884 5444 1132
Size: 2752 1560 3774
Fragments arranged by size:
3774 2752 1560
SspI AAT'ATT
Cuts at: 5 52 3833 6010 5
Size: 47 3781 2177 2081
Fragments arranged by size:
3781 2177 2081 47
StuI AGG'CCT
Cuts at: 1517 3216 1517
Size: 1699 6387
StyI C'CwwG_G
Cuts at: 513 1065 2004 5525 513
Size: 552 939 3521 3074
Fragments arranged by size:
3521 3074 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4072 6113 6130 6283 6468 6913 325
Size: 1255 2492 2041 17 153 185 445 1498
Fragments arranged by size:
2492 2041 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2654 2836 4235
Size: 274 80 33 51 1443 677 182 1399
Cuts at: 4235 4244 5217 5374 6332 8044 96
Size: 9 973 157 958 1712 138
Fragments arranged by size:
1712 1443 1399 973 958 677 274 182
157 138 80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4141 4173 8046 8078 4141
Size: 32 3873 32 4149
Fragments arranged by size:
4149 3873 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8086
XbaI T'CTAG_A
Cuts at: 4474 5422 4474
Size: 948 7138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4665 4776 4665
Size: 111 7975
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8086
XmaI C'CCGG_G
Cuts at: 1870 2500 5411 1870
Size: 630 2911 4545
Fragments arranged by size:
4545 2911 630
XmnI GAAnn'nnTTC
Cuts at: 2861 2949 4032 4908 6215 2861
Size: 88 1083 876 1307 4732
Fragments arranged by size:
4732 1307 1083 876 88
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5894 276
Size: 53 83 186 936 4360 2468
Fragments arranged by size:
4360 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI
NruI PacI PmlI PspXI PsrI RsrII SacI SapI
SexAI SfiI SfoI SrfI SwaI