Restriction map for
pFN21AA1111
(Circular) (Six-base) MAPSORT of: pFN21AA1111.seq Check: 2347 from: 1 to: 7885
pFN21AA1111 7885 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4201 4201
Size: 7885
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5695 278
Size: 53 83 186 936 4159 2468
Fragments arranged by size:
4159 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3900 5228 1937
Size: 1963 1328 4594
Fragments arranged by size:
4594 1963 1328
Acc65I G'GTAC_C
Cuts at: 2880 5206 2880
Size: 2326 5559
AclI AA'CG_TT
Cuts at: 1563 3453 6012 6385 1563
Size: 1890 2559 373 3063
Fragments arranged by size:
3063 2559 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 2458 2516 3172 3522 3617 3731 4832
Size: 812 14 58 656 350 95 114 1101
Cuts at: 4832 5049 5948 7332 7600 1632
Size: 217 899 1384 268 1917
Fragments arranged by size:
1917 1384 1101 899 812 656 350 268
217 114 95 58 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7688
AflIII A'CryG_T
Cuts at: 1203 2038 2184 6784 7761 1203
Size: 835 146 4600 977 1327
Fragments arranged by size:
4600 1327 977 835 146
AgeI A'CCGG_T
Cuts at: 6716 6716
Size: 7885
AhdI GACnn_n'nnGTC
Cuts at: 6614 6614
Size: 7885
AleI CACnn'nnGTG
Cuts at: 1166 3539 1166
Size: 2373 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2537 1184
Size: 1353 6532
AlwNI CAG_nnn'CTG
Cuts at: 3709 4742 5628 7200 3709
Size: 1033 886 1572 4394
Fragments arranged by size:
4394 1572 1033 886
ApaI G_GGCC'C
Cuts at: 2289 2289
Size: 7885
ApaLI G'TGCA_C
Cuts at: 5942 7098 5942
Size: 1156 6729
ApoI r'AATT_y
Cuts at: 1493 2453 2693 3941 4022 4621 5194 5420 5572
Size: 960 240 1248 81 599 573 226 152
Cuts at: 5572 7681 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1248 960 599 573 240 226
152 81
AseI AT'TA_AT
Cuts at: 160 1032 6439 160
Size: 872 5407 1606
Fragments arranged by size:
5407 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7885
AvaI C'yCGr_G
Cuts at: 1870 1943 4704 5210 1870
Size: 73 2761 506 4545
Fragments arranged by size:
4545 2761 506 73
BamHI G'GATC_C
Cuts at: 3063 5215 5582 3063
Size: 2152 367 5366
Fragments arranged by size:
5366 2152 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2867 2880 3752 5058
Size: 325 217 627 335 745 13 872 1306
Cuts at: 5058 5206 6661 618
Size: 148 1455 1842
Fragments arranged by size:
1842 1455 1306 872 745 627 335 325
217 148 13
BanII G_rGCy'C
Cuts at: 1391 2289 2366 2385 3815 4385 5204 1391
Size: 898 77 19 1430 570 819 4072
Fragments arranged by size:
4072 1430 898 819 570 77 19
BbeI G_GCGC'C
Cuts at: 5062 5062
Size: 7885
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 3441 3467 3719 3942 5035 928
Size: 971 461 1081 26 252 223 1093 3778
Fragments arranged by size:
3778 1093 1081 971 461 252 223 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6076 6110 1903
Size: 34 4139 34 3678
Fragments arranged by size:
4139 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5159 5777 6987 5159
Size: 618 1210 6057
Fragments arranged by size:
6057 1210 618
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7885
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6496 136
Size: 107 122 71 631 605 4824 1525
Fragments arranged by size:
4824 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2904 3406 7880 2904
Size: 502 4474 2909
Fragments arranged by size:
4474 2909 502
BlpI GC'TnA_GC
Cuts at: 3170 5302 3170
Size: 2132 5753
Bme1580I G_kGCm'C
Cuts at: 1790 2289 2484 4403 5946 7102 1790
Size: 499 195 1919 1543 1156 2573
Fragments arranged by size:
2573 1919 1543 1156 499 195
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7885
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4365 5616 6574
Size: 416 53 83 399 24 2948 1251 958
Cuts at: 6574 442
Size: 1753
Fragments arranged by size:
2948 1753 1251 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2977 4369 4489 4589 6545 1239
Size: 842 896 1392 120 100 1956 2579
Fragments arranged by size:
2579 1956 1392 896 842 120 100
Bpu10I CC'TnA_GC
Cuts at: 4926 4926
Size: 7885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2876 4181 4821 6010 6875 7173 2876
Size: 1305 640 1189 865 298 3588
Fragments arranged by size:
3588 1305 1189 865 640 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2788 3200 6548 882
Size: 617 1289 412 3348 2219
Fragments arranged by size:
3348 2219 1289 617 412
BsaAI yAC'GTr
Cuts at: 493 1204 5676 493
Size: 711 4472 2702
Fragments arranged by size:
4472 2702 711
BsaBI GATnn'nnATC
Cuts at: 7879 7879
Size: 7885
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3080 5059 5692 6074
Size: 53 83 186 936 1547 1979 633 382
Cuts at: 6074 6709 275
Size: 635 1451
Fragments arranged by size:
1979 1547 1451 936 635 633 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2385 6318 6716 6990 7137 7548 2385
Size: 3933 398 274 147 411 2722
Fragments arranged by size:
3933 2722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3266 3296 1178
Size: 30 165 30 1863 30 5767
Fragments arranged by size:
5767 1863 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 4121 4499 4502 4505 4508 4894 4897
Size: 1057 1878 378 3 3 3 386 3
Cuts at: 4897 4911 1186
Size: 14 4160
Fragments arranged by size:
4160 1878 1057 386 378 14 3 3
3 3
BseYI C'CCAG_C
Cuts at: 3496 4249 4259 4396 4425 4755 5052 7088 7584
Size: 753 10 137 29 330 297 2036 496
Cuts at: 7584 3496
Size: 3797
Fragments arranged by size:
3797 2036 753 496 330 297 137 29
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2086 5146 5335 6096 7124 1540
Size: 275 186 85 3060 189 761 1028 2301
Fragments arranged by size:
3060 2301 1028 761 275 189 186 85
BsiHKAI G_wGCw'C
Cuts at: 2385 3176 4385 5946 6031 7102 2385
Size: 791 1209 1561 85 1071 3168
Fragments arranged by size:
3168 1561 1209 1071 791 85
BsmI GAATG_Cn'
Cuts at: 4182 5396 5489 4182
Size: 1214 93 6578
Fragments arranged by size:
6578 1214 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 2366 2385 2484 3176 3815 4385
Size: 399 499 77 19 99 692 639 570
Cuts at: 4385 4403 5204 5946 6031 7102 1391
Size: 18 801 742 85 1071 2174
Fragments arranged by size:
2174 1071 801 742 692 639 570 499
399 99 85 77 19 18
BspEI T'CCGG_A
Cuts at: 2385 7548 2385
Size: 5163 2722
BspHI T'CATG_A
Cuts at: 2169 2820 5774 7504 2169
Size: 651 2954 1730 2550
Fragments arranged by size:
2954 2550 1730 651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4201 4311 5240 844
Size: 3357 110 929 3489
Fragments arranged by size:
3489 3357 929 110
BsrBI CCG'CTC
Cuts at: 1394 1823 4979 5772 1394
Size: 429 3156 793 3507
Fragments arranged by size:
3507 3156 793 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6380 6554 65
Size: 1183 5132 174 1396
Fragments arranged by size:
5132 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 2125 2142 3500 4324 6529 6716
Size: 110 271 44 17 1358 824 2205 187
Cuts at: 6716 1700
Size: 2869
Fragments arranged by size:
2869 2205 1358 824 271 187 110 44
17
BsrGI T'GTAC_A
Cuts at: 96 2803 96
Size: 2707 5178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7885
BssSI C'ACGA_G
Cuts at: 5945 6957 5945
Size: 1012 6873
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4290 4742 794
Size: 3496 452 3937
Fragments arranged by size:
3937 3496 452
BstBI TT'CG_AA
Cuts at: 2743 6689 2743
Size: 3946 3939
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7885
BstXI CCAn_nnnn'nTGG
Cuts at: 4467 5029 5168 4467
Size: 562 139 7184
Fragments arranged by size:
7184 562 139
BstYI r'GATC_y
Cuts at: 1972 2312 2904 3063 3369 3406 3420 3489 5215
Size: 340 592 159 306 37 14 69 1726
Cuts at: 5215 5582 5967 5984 7436 7522 7534 7880 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1726 1452 592 385 367 346 340
306 159 86 69 37 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2308 2448 3295 4637 513
Size: 552 1243 140 847 1342 3761
Fragments arranged by size:
3761 1342 1243 847 552 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7146
Fragments arranged by size:
7146 683 56
BtsI GCAGTG_nn'
Cuts at: 2257 3560 4381 4396 5401 6195 6215 2257
Size: 1303 821 15 1005 794 20 3927
Fragments arranged by size:
3927 1303 1005 821 794 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7885
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7850
DraI TTT'AAA
Cuts at: 5189 5189
Size: 7885
DraIII CAC_nnn'GTG
Cuts at: 3060 3060
Size: 7885
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6892 809
Size: 507 5576 1802
Fragments arranged by size:
5576 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2083 2771
Size: 54 1425 50 275 18 249 4 688
Cuts at: 2771 3411 3664 4296 4457 5121 5143 5332 6221
Size: 640 253 632 161 664 22 189 889
Cuts at: 6221 7850 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 688 664 640 632 275
253 249 189 161 54 50 43 22
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2083 5143 5332 1537
Size: 275 271 3060 189 4090
Fragments arranged by size:
4090 3060 275 271 189
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 2654 3191 3617 3851 5815 1353
Size: 867 434 537 426 234 1964 3423
Fragments arranged by size:
3423 1964 867 537 434 426 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2909 3413 6472 6846 6992 387
Size: 1064 138 1320 504 3059 374 146 1280
Fragments arranged by size:
3059 1320 1280 1064 504 374 146 138
EcoICRI GAG'CTC
Cuts at: 2383 4383 2383
Size: 2000 5885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2444 2458 2516 2977 3172 3522
Size: 393 449 363 14 58 461 195 350
Cuts at: 3522 3617 3731 4369 4489 4589 4832 5049 5948
Size: 95 114 638 120 100 243 217 899
Cuts at: 5948 6545 7332 7600 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 899 787 638 597 461 449 393
363 350 268 243 217 195 120 114
100 95 58 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 3334 4241 2498
Size: 836 907 6142
Fragments arranged by size:
6142 907 836
EcoO109I rG'GnC_Cy
Cuts at: 2286 3656 4220 4246 4701 4942 5116 2286
Size: 1370 564 26 455 241 174 5055
Fragments arranged by size:
5055 1370 564 455 241 174 26
EcoRI G'AATT_C
Cuts at: 2453 2693 4621 5194 5572 2453
Size: 240 1928 573 378 4766
Fragments arranged by size:
4766 1928 573 378 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3628 4172 3628
Size: 544 7341
FseI GG_CCGG'CC
Cuts at: 2085 2085
Size: 7885
FspI TGC'GCA
Cuts at: 6391 6391
Size: 7885
HaeII r_GCGC'y
Cuts at: 1678 4176 5062 7032 1678
Size: 2498 886 1970 2531
Fragments arranged by size:
2531 2498 1970 886
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3664 3696 3764 3796 4651
Size: 32 135 32 2092 32 68 32 855
Cuts at: 4651 4683 4931 4963 6526 6558 6600 6632 1373
Size: 32 248 32 1563 32 42 32 2626
Fragments arranged by size:
2626 2092 1563 855 248 135 68 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5229 5475 669
Size: 988 281 3291 246 3079
Fragments arranged by size:
3291 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2624 4154 748
Size: 1876 1530 4479
Fragments arranged by size:
4479 1876 1530
HpaI GTT'AAC
Cuts at: 5475 5475
Size: 7885
KasI G'GCGC_C
Cuts at: 5058 5058
Size: 7885
KpnI G_GTAC'C
Cuts at: 2884 5210 2884
Size: 2326 5559
MfeI C'AATT_G
Cuts at: 5484 5484
Size: 7885
MluI A'CGCG_T
Cuts at: 2038 7761 2038
Size: 5723 2162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3469 6999 7183 1305
Size: 2164 3530 184 2007
Fragments arranged by size:
3530 2164 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2773 3413 3666 4298 5123
Size: 54 1425 343 941 640 253 632 825
Cuts at: 5123 10
Size: 2772
Fragments arranged by size:
2772 1425 941 825 640 632 343 253
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2825 3134 3363 3539 5027 5166 5843
Size: 648 1659 309 229 176 1488 139 677
Cuts at: 5843 6202 6361 518
Size: 359 159 2042
Fragments arranged by size:
2042 1659 1488 677 648 359 309 229
176 159 139
MspA1I CmG'CkG
Cuts at: 1606 2655 4742 5056 5301 5978 7126 7371 1606
Size: 1049 2087 314 245 677 1148 245 2120
Fragments arranged by size:
2120 2087 1148 1049 677 314 245 245
NaeI GCC'GGC
Cuts at: 2083 2144 3502 2083
Size: 61 1358 6466
Fragments arranged by size:
6466 1358 61
NarI GG'CG_CC
Cuts at: 5059 5059
Size: 7885
NcoI C'CATG_G
Cuts at: 513 1065 3295 4637 513
Size: 552 2230 1342 3761
Fragments arranged by size:
3761 2230 1342 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7885
NgoMIV G'CCGG_C
Cuts at: 2081 2142 3500 2081
Size: 61 1358 6466
Fragments arranged by size:
6466 1358 61
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7885
NotI GC'GGCC_GC
Cuts at: 5332 5332
Size: 7885
NspI r_CATG'y
Cuts at: 1132 1229 2188 4288 5243 6788 1132
Size: 97 959 2100 955 1545 2229
Fragments arranged by size:
2229 2100 1545 959 955 97
PasI CC'CwG_GG
Cuts at: 1348 4436 1348
Size: 3088 4797
PciI A'CATG_T
Cuts at: 2184 6784 2184
Size: 4600 3285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3060 3216 1108
Size: 1952 156 5777
Fragments arranged by size:
5777 1952 156
PfoI T'CCnGG_A
Cuts at: 2457 3237 3372 2457
Size: 780 135 6970
Fragments arranged by size:
6970 780 135
PmeI GTTT'AAAC
Cuts at: 5189 5189
Size: 7885
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5954 5986 7467 7499 5954
Size: 32 1481 32 6340
Fragments arranged by size:
6340 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4220 4701 4220
Size: 481 7404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7885
PsiI TTA'TAA
Cuts at: 5455 5455
Size: 7885
PspOMI G'GGCC_C
Cuts at: 2285 2285
Size: 7885
PstI C_TGCA'G
Cuts at: 830 3486 3972 4308 4344 4738 5237 830
Size: 2656 486 336 36 394 499 3478
Fragments arranged by size:
3478 2656 499 486 394 336 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7885
PvuII CAG'CTG
Cuts at: 4742 4742
Size: 7885
SacI G_AGCT'C
Cuts at: 2385 4385 2385
Size: 2000 5885
SalI G'TCGA_C
Cuts at: 1936 5227 1936
Size: 3291 4594
SanDI GG'GwC_CC
Cuts at: 4701 4701
Size: 7885
SbfI CC_TGCA'GG
Cuts at: 4344 5237 4344
Size: 893 6992
ScaI AGT'ACT
Cuts at: 2813 6133 7845 2813
Size: 3320 1712 2853
Fragments arranged by size:
3320 2853 1712
SexAI A'CCwGG_T
Cuts at: 2997 3288 2997
Size: 291 7594
SfcI C'TryA_G
Cuts at: 826 1045 3482 3968 4304 4340 4734 4766 5233
Size: 219 2437 486 336 36 394 32 467
Cuts at: 5233 6368 7049 7240 826
Size: 1135 681 191 1471
Fragments arranged by size:
2437 1471 1135 681 486 467 394 336
219 191 36 32
SfoI GGC'GCC
Cuts at: 5060 5060
Size: 7885
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7885
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7885
SmaI CCC'GGG
Cuts at: 1872 4706 5212 1872
Size: 2834 506 4545
Fragments arranged by size:
4545 2834 506
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 4196 4836 5989 6890 7152
Size: 197 926 948 1305 640 1153 901 262
Cuts at: 7152 820
Size: 1553
Fragments arranged by size:
1553 1305 1153 948 926 901 640 262
197
SnaBI TAC'GTA
Cuts at: 493 5676 493
Size: 5183 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7885
SphI G_CATG'C
Cuts at: 1132 4288 5243 1132
Size: 3156 955 3774
Fragments arranged by size:
3774 3156 955
SspI AAT'ATT
Cuts at: 5 52 5809 5
Size: 47 5757 2081
Fragments arranged by size:
5757 2081 47
StuI AGG'CCT
Cuts at: 1517 3594 4346 1517
Size: 2077 752 5056
Fragments arranged by size:
5056 2077 752
StyI C'CwwG_G
Cuts at: 513 1065 3295 3603 3651 4637 5324 513
Size: 552 2230 308 48 986 687 3074
Fragments arranged by size:
3074 2230 986 687 552 308 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5912 5929 6082 6267 6712 325
Size: 1255 4332 17 153 185 445 1498
Fragments arranged by size:
4332 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2803 2811 2887
Size: 274 80 33 51 1443 826 8 76
Cuts at: 2887 4971 6131 7843 96
Size: 2084 1160 1712 138
Fragments arranged by size:
2084 1712 1443 1160 826 274 138 80
76 51 33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 4153 4185 4966 4998 7845 7877 3266
Size: 32 855 32 781 32 2847 32 3274
Fragments arranged by size:
3274 2847 855 781 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7885
XbaI T'CTAG_A
Cuts at: 5221 5221
Size: 7885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3452 4210 4393 5116 3452
Size: 758 183 723 6221
Fragments arranged by size:
6221 758 723 183
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7885
XmaI C'CCGG_G
Cuts at: 1870 4704 5210 1870
Size: 2834 506 4545
Fragments arranged by size:
4545 2834 506
XmnI GAAnn'nnTTC
Cuts at: 2418 6014 2418
Size: 3596 4289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5693 276
Size: 53 83 186 936 4159 2468
Fragments arranged by size:
4159 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SfiI
SrfI SwaI