Restriction map for
pFN21AA1106
(Circular) (Six-base) MAPSORT of: pFN21AA1106.seq Check: 3210 from: 1 to: 8101
pFN21AA1106 8101 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5911 278
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4305 5318 5444 1937
Size: 2368 1013 126 4594
Fragments arranged by size:
4594 2368 1013 126
Acc65I G'GTAC_C
Cuts at: 4806 5422 4806
Size: 616 7485
AclI AA'CG_TT
Cuts at: 1563 6228 6601 1563
Size: 4665 373 3063
Fragments arranged by size:
4665 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2313 3129 5162 6164 7548 7816 1632
Size: 681 816 2033 1002 1384 268 1917
Fragments arranged by size:
2033 1917 1384 1002 816 681 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7904
AflIII A'CryG_T
Cuts at: 1203 3621 4749 7000 7977 1203
Size: 2418 1128 2251 977 1327
Fragments arranged by size:
2418 2251 1327 1128 977
AgeI A'CCGG_T
Cuts at: 4260 6932 4260
Size: 2672 5429
AhdI GACnn_n'nnGTC
Cuts at: 2713 6830 2713
Size: 4117 3984
AleI CACnn'nnGTG
Cuts at: 1166 2154 3089 1166
Size: 988 935 6178
Fragments arranged by size:
6178 988 935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8101
AlwNI CAG_nnn'CTG
Cuts at: 4090 4111 4200 4987 5224 5844 7416 4090
Size: 21 89 787 237 620 1572 4775
Fragments arranged by size:
4775 1572 787 620 237 89 21
ApaLI G'TGCA_C
Cuts at: 6158 7314 6158
Size: 1156 6945
ApoI r'AATT_y
Cuts at: 1493 5281 5391 5410 5636 5788 7897 1493
Size: 3788 110 19 226 152 2109 1697
Fragments arranged by size:
3788 2109 1697 226 152 110 19
AseI AT'TA_AT
Cuts at: 160 1032 6655 160
Size: 872 5623 1606
Fragments arranged by size:
5623 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
AvaI C'yCGr_G
Cuts at: 1870 1943 3269 3368 3755 4412 4712 4936 5426
Size: 73 1326 99 387 657 300 224 490
Cuts at: 5426 1870
Size: 4545
Fragments arranged by size:
4545 1326 657 490 387 300 224 99
73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4430 4463 4430
Size: 33 8068
BamHI G'GATC_C
Cuts at: 5263 5431 5798 5263
Size: 168 367 7566
Fragments arranged by size:
7566 367 168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4773 4806 5422 6877 618
Size: 325 217 627 2986 33 616 1455 1842
Fragments arranged by size:
2986 1842 1455 627 616 325 217 33
BanII G_rGCy'C
Cuts at: 1391 3020 3756 4227 5420 1391
Size: 1629 736 471 1193 4072
Fragments arranged by size:
4072 1629 1193 736 471
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 2361 2556 4374 5150 928
Size: 971 402 60 195 1818 776 3879
Fragments arranged by size:
3879 1818 971 776 402 195 60
BbvCI CC'TCA_GC
Cuts at: 2484 4166 2484
Size: 1682 6419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6292 6326 1903
Size: 34 4355 34 3678
Fragments arranged by size:
4355 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4419 5993 7203 4419
Size: 1574 1210 5317
Fragments arranged by size:
5317 1574 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8101
BfrBI ATG'CAT
Cuts at: 3472 3472
Size: 8101
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2501 4034 6712
Size: 107 122 71 631 605 829 1533 2678
Cuts at: 6712 136
Size: 1525
Fragments arranged by size:
2678 1533 1525 829 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8096 8096
Size: 8101
BlpI GC'TnA_GC
Cuts at: 5518 5518
Size: 8101
Bme1580I G_kGCm'C
Cuts at: 1790 3494 4559 4778 6162 7318 1790
Size: 1704 1065 219 1384 1156 2573
Fragments arranged by size:
2573 1704 1384 1156 1065 219
BmgBI CAC'GTC
Cuts at: 1318 3622 4894 4947 1318
Size: 2304 1272 53 4472
Fragments arranged by size:
4472 2304 1272 53
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4749 4816
Size: 416 53 83 399 24 1988 1344 67
Cuts at: 4816 5832 6790 442
Size: 1016 958 1753
Fragments arranged by size:
1988 1753 1344 1016 958 416 399 83
67 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5238 5238
Size: 8101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2849 3023 3170 3438 3704 4241 4347 4995
Size: 1610 174 147 268 266 537 106 648
Cuts at: 4995 6761 1239
Size: 1766 2579
Fragments arranged by size:
2579 1766 1610 648 537 268 266 174
147 106
Bpu10I CC'TnA_GC
Cuts at: 2484 3656 3950 4091 4166 4885 5213 2484
Size: 1172 294 141 75 719 328 5372
Fragments arranged by size:
5372 1172 719 328 294 141 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3316 3470 4535 6226 7091 7389 3316
Size: 154 1065 1691 865 298 4028
Fragments arranged by size:
4028 1691 1065 865 298 154
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2870 6764 882
Size: 617 1371 3894 2219
Fragments arranged by size:
3894 2219 1371 617
BsaAI yAC'GTr
Cuts at: 493 1204 3391 3984 5296 5892 493
Size: 711 2187 593 1312 596 2702
Fragments arranged by size:
2702 2187 1312 711 596 593
BsaBI GATnn'nnATC
Cuts at: 5334 8095 5334
Size: 2761 5340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2960 5908 6290 6925
Size: 53 83 186 936 1427 2948 382 635
Cuts at: 6925 275
Size: 1451
Fragments arranged by size:
2948 1451 1427 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3425 3557 4260 4705 6534 6932 7206 7353 7764
Size: 132 703 445 1829 398 274 147 411
Cuts at: 7764 3425
Size: 3762
Fragments arranged by size:
3762 1829 703 445 411 398 274 147
132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2996 3026 3044 3074 3524
Size: 30 165 30 1593 30 18 30 450
Cuts at: 3524 3554 4214 4244 1178
Size: 30 660 30 5035
Fragments arranged by size:
5035 1593 660 450 165 30 30 30
30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2248 2327 2342 2345 2810 2822 3020
Size: 850 212 79 15 3 465 12 198
Cuts at: 3020 3235 5171 5183 1186
Size: 215 1936 12 4104
Fragments arranged by size:
4104 1936 850 465 215 212 198 79
15 12 12 3
BseYI C'CCAG_C
Cuts at: 3215 3896 3902 3932 4236 4273 4290 7304 7800
Size: 681 6 30 304 37 17 3014 496
Cuts at: 7800 3215
Size: 3516
Fragments arranged by size:
3516 3014 681 496 304 37 30 17
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3490 4006 1163
Size: 573 1754 516 5258
Fragments arranged by size:
5258 1754 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3026 3570 5551 6312 7340 1540
Size: 275 186 1025 544 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 1025 761 544 275 186
BsiHKAI G_wGCw'C
Cuts at: 2676 3657 3756 4097 6162 6247 7318 2676
Size: 981 99 341 2065 85 1071 3459
Fragments arranged by size:
3459 2065 1071 981 341 99 85
BsmI GAATG_Cn'
Cuts at: 4515 5612 5705 4515
Size: 1097 93 6911
Fragments arranged by size:
6911 1097 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2676 3020 3494 3657 3756 4097 4227
Size: 399 886 344 474 163 99 341 130
Cuts at: 4227 4559 4778 5420 6162 6247 7318 1391
Size: 332 219 642 742 85 1071 2174
Fragments arranged by size:
2174 1071 886 742 642 474 399 344
341 332 219 163 130 99 85
BspEI T'CCGG_A
Cuts at: 3425 3557 7764 3425
Size: 132 4207 3762
Fragments arranged by size:
4207 3762 132
BspHI T'CATG_A
Cuts at: 3064 5990 7720 3064
Size: 2926 1730 3445
Fragments arranged by size:
3445 2926 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 5456 844
Size: 1380 3232 3489
Fragments arranged by size:
3489 3232 1380
BsrBI CCG'CTC
Cuts at: 1394 1823 3070 5988 1394
Size: 429 1247 2918 3507
Fragments arranged by size:
3507 2918 1247 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6596 6770 65
Size: 1183 5348 174 1396
Fragments arranged by size:
5348 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3383 4260 4592 6745 6932 1700
Size: 110 1573 877 332 2153 187 2869
Fragments arranged by size:
2869 2153 1573 877 332 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8101
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8101
BssSI C'ACGA_G
Cuts at: 5198 6161 7173 5198
Size: 963 1012 6126
Fragments arranged by size:
6126 1012 963
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3984 4860 794
Size: 3190 876 4035
Fragments arranged by size:
4035 3190 876
BstBI TT'CG_AA
Cuts at: 4519 6905 4519
Size: 2386 5715
BstEII G'GTnAC_C
Cuts at: 1184 4420 1184
Size: 3236 4865
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2545 3166 3395 3809 4739 2011
Size: 534 621 229 414 930 5373
Fragments arranged by size:
5373 930 621 534 414 229
BstYI r'GATC_y
Cuts at: 1972 5263 5431 5798 6183 6200 7652 7738 7750
Size: 3291 168 367 385 17 1452 86 12
Cuts at: 7750 8096 1972
Size: 346 1977
Fragments arranged by size:
3291 1977 1452 385 367 346 168 86
17 12
BstZ17I GTA'TAC
Cuts at: 5319 5319
Size: 8101
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 3207 4187 513
Size: 552 939 60 1143 980 4427
Fragments arranged by size:
4427 1143 980 939 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2018 2817 3145 4742 4967 487
Size: 683 56 792 799 328 1597 225 3621
Fragments arranged by size:
3621 1597 799 792 683 328 225 56
BtsI GCAGTG_nn'
Cuts at: 2125 4254 5617 6411 6431 2125
Size: 2129 1363 794 20 3795
Fragments arranged by size:
3795 2129 1363 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8066
DraI TTT'AAA
Cuts at: 5405 5405
Size: 8101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3305 7108 809
Size: 507 1989 3803 1802
Fragments arranged by size:
3803 1989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2451 2859 2933
Size: 54 1425 50 275 18 621 408 74
Cuts at: 2933 3204 3385 3588 3744 4038 4530 4723 4785
Size: 271 181 203 156 294 492 193 62
Cuts at: 4785 5548 6437 8066 8
Size: 763 889 1629 43
Fragments arranged by size:
1629 1425 889 763 621 492 408 294
275 271 203 193 181 156 74 62
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5548 1537
Size: 275 3736 4090
Fragments arranged by size:
4090 3736 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 2934 3105 5148 6031 1353
Size: 978 603 171 2043 883 3423
Fragments arranged by size:
3423 2043 978 883 603 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4520 6688 7062 7208 387
Size: 1064 138 2931 2168 374 146 1280
Fragments arranged by size:
2931 2168 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3754 3754
Size: 8101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2313 2849 3023 3129 3170 3438 3704
Size: 393 681 536 174 106 41 268 266
Cuts at: 3704 4241 4347 4995 5162 6164 6761 7548 7816
Size: 537 106 648 167 1002 597 787 268
Cuts at: 7816 1239
Size: 1524
Fragments arranged by size:
1524 1002 787 681 648 597 537 536
393 268 268 266 174 167 106 106
41
EcoNI CCTnn'n_nnAGG
Cuts at: 3746 3746
Size: 8101
EcoO109I rG'GnC_Cy
Cuts at: 3448 3892 4108 4155 3448
Size: 444 216 47 7394
Fragments arranged by size:
7394 444 216 47
EcoRI G'AATT_C
Cuts at: 5391 5410 5788 5391
Size: 19 378 7704
Fragments arranged by size:
7704 378 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4015 4015
Size: 8101
FspI TGC'GCA
Cuts at: 6607 6607
Size: 8101
HaeII r_GCGC'y
Cuts at: 1678 3975 4767 7248 1678
Size: 2297 792 2481 2531
Fragments arranged by size:
2531 2481 2297 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2699 2731 3071
Size: 32 135 32 671 32 424 32 340
Cuts at: 3071 3103 3236 3268 3343 3375 3552 3584 4214
Size: 32 133 32 75 32 177 32 630
Cuts at: 4214 4246 4331 4363 6742 6774 6816 6848 1373
Size: 32 85 32 2379 32 42 32 2626
Fragments arranged by size:
2626 2379 671 630 424 340 177 135
133 85 75 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3526 5445 5691 669
Size: 988 281 1588 1919 246 3079
Fragments arranged by size:
3079 1919 1588 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4967 748
Size: 4219 3882
HpaI GTT'AAC
Cuts at: 5691 5691
Size: 8101
KpnI G_GTAC'C
Cuts at: 4810 5426 4810
Size: 616 7485
MfeI C'AATT_G
Cuts at: 5700 5700
Size: 8101
MluI A'CGCG_T
Cuts at: 4749 7977 4749
Size: 3228 4873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2561 3386 3762 7215 7399 1305
Size: 1256 825 376 3453 184 2007
Fragments arranged by size:
3453 2007 1256 825 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 2861 3206 3590 3746
Size: 54 1425 343 621 408 345 384 156
Cuts at: 3746 4532 10
Size: 786 3579
Fragments arranged by size:
3579 1425 786 621 408 384 345 343
156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2130 2154 3089 3477 3807 4139
Size: 648 843 121 24 935 388 330 332
Cuts at: 4139 4316 6059 6418 6577 518
Size: 177 1743 359 159 2042
Fragments arranged by size:
2042 1743 935 843 648 388 359 332
330 177 159 121 24
MspA1I CmG'CkG
Cuts at: 1606 2094 3042 3682 4189 4197 4273 4899 5002
Size: 488 948 640 507 8 76 626 103
Cuts at: 5002 5251 5517 6194 7342 7587 1606
Size: 249 266 677 1148 245 2120
Fragments arranged by size:
2120 1148 948 677 640 626 507 488
266 249 245 103 76 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 3207 513
Size: 552 939 1203 5407
Fragments arranged by size:
5407 1203 939 552
NdeI CA'TA_TG
Cuts at: 387 3241 387
Size: 2854 5247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8101
NotI GC'GGCC_GC
Cuts at: 5548 5548
Size: 8101
NsiI A_TGCA'T
Cuts at: 3474 3474
Size: 8101
NspI r_CATG'y
Cuts at: 1132 1229 2716 2930 2986 3524 4858 5459 7004
Size: 97 1487 214 56 538 1334 601 1545
Cuts at: 7004 1132
Size: 2229
Fragments arranged by size:
2229 1545 1487 1334 601 538 214 97
56
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8101
PciI A'CATG_T
Cuts at: 7000 7000
Size: 8101
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5203 5236 1108
Size: 4095 33 3973
Fragments arranged by size:
4095 3973 33
PfoI T'CCnGG_A
Cuts at: 4383 4829 4383
Size: 446 7655
PmeI GTTT'AAAC
Cuts at: 5405 5405
Size: 8101
PmlI CAC'GTG
Cuts at: 3984 3984
Size: 8101
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6170 6202 7683 7715 6170
Size: 32 1481 32 6556
Fragments arranged by size:
6556 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3448 3892 4108 4155 3448
Size: 444 216 47 7394
Fragments arranged by size:
7394 444 216 47
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8101
PsiI TTA'TAA
Cuts at: 5671 5671
Size: 8101
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2061 2093 5104 5136 2061
Size: 32 3011 32 5026
Fragments arranged by size:
5026 3011 32 32
PstI C_TGCA'G
Cuts at: 830 3577 5453 830
Size: 2747 1876 3478
Fragments arranged by size:
3478 2747 1876
PvuI CG_AT'CG
Cuts at: 2001 3570 2001
Size: 1569 6532
PvuII CAG'CTG
Cuts at: 2094 3042 3682 4197 4273 5251 2094
Size: 948 640 515 76 978 4944
Fragments arranged by size:
4944 978 948 640 515 76
RsrII CG'GwC_CG
Cuts at: 3646 3646
Size: 8101
SacI G_AGCT'C
Cuts at: 3756 3756
Size: 8101
SacII CC_GC'GG
Cuts at: 4190 4190
Size: 8101
SalI G'TCGA_C
Cuts at: 1936 5443 1936
Size: 3507 4594
SanDI GG'GwC_CC
Cuts at: 3448 4155 3448
Size: 707 7394
SbfI CC_TGCA'GG
Cuts at: 5453 5453
Size: 8101
ScaI AGT'ACT
Cuts at: 6349 8061 6349
Size: 1712 6389
SfcI C'TryA_G
Cuts at: 826 1045 3573 3703 5449 6584 7265 7456 826
Size: 219 2528 130 1746 1135 681 191 1471
Fragments arranged by size:
2528 1746 1471 1135 681 219 191 130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8101
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8101
SmaI CCC'GGG
Cuts at: 1872 3271 3370 4414 4714 5428 1872
Size: 1399 99 1044 300 714 4545
Fragments arranged by size:
4545 1399 1044 714 300 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3331 3485 3755 4550 6205 7106
Size: 197 926 1388 154 270 795 1655 901
Cuts at: 7106 7368 820
Size: 262 1553
Fragments arranged by size:
1655 1553 1388 926 901 795 270 262
197 154
SnaBI TAC'GTA
Cuts at: 493 5892 493
Size: 5399 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8101
SphI G_CATG'C
Cuts at: 1132 5459 1132
Size: 4327 3774
SspI AAT'ATT
Cuts at: 5 52 3764 6025 5
Size: 47 3712 2261 2081
Fragments arranged by size:
3712 2261 2081 47
StuI AGG'CCT
Cuts at: 1517 3641 1517
Size: 2124 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 2656 3129 3207 3591 3747
Size: 552 939 168 484 473 78 384 156
Cuts at: 3747 3831 4779 5540 513
Size: 84 948 761 3074
Fragments arranged by size:
3074 948 939 761 552 484 473 384
168 156 84 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3048 4828 6128 6145 6298 6483 6928
Size: 1255 1468 1780 1300 17 153 185 445
Cuts at: 6928 325
Size: 1498
Fragments arranged by size:
1780 1498 1468 1300 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6347 8059 96
Size: 274 80 33 51 1443 4370 1712 138
Fragments arranged by size:
4370 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2857 2889 3044 3076 8061 8093 2857
Size: 32 155 32 4985 32 2865
Fragments arranged by size:
4985 2865 155 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2512 1316
Size: 1196 6905
XbaI T'CTAG_A
Cuts at: 4393 5437 4393
Size: 1044 7057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5083 5083
Size: 8101
XhoI C'TCGA_G
Cuts at: 1943 3755 1943
Size: 1812 6289
XmaI C'CCGG_G
Cuts at: 1870 3269 3368 4412 4712 5426 1870
Size: 1399 99 1044 300 714 4545
Fragments arranged by size:
4545 1399 1044 714 300 99
XmnI GAAnn'nnTTC
Cuts at: 6230 6230
Size: 8101
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5909 276
Size: 53 83 186 936 4375 2468
Fragments arranged by size:
4375 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BsmBI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PspOMI PspXI SapI SexAI
SfiI SfoI SrfI SwaI