Restriction map for
pFN21AA1101
(Circular) (Six-base) MAPSORT of: pFN21AA1101.seq Check: 6962 from: 1 to: 6286
pFN21AA1101 6286 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3426 3629 1937
Size: 1489 203 4594
Fragments arranged by size:
4594 1489 203
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2610 3255 4349 5733 6001 1632
Size: 978 645 1094 1384 268 1917
Fragments arranged by size:
1917 1384 1094 978 645 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 5185 6162 1203
Size: 3982 977 1327
Fragments arranged by size:
3982 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 2346 1166
Size: 1180 5106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 2286
Size: 32 6254
AlwNI CAG_nnn'CTG
Cuts at: 4029 5601 4029
Size: 1572 4714
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 2193 2870 2897 3029 3106 3328 3362 3595
Size: 700 677 27 132 77 222 34 233
Cuts at: 3595 3821 3973 6082 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 700 677 233 226 222 152
132 77 34 27
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 3084 3611 1870
Size: 73 74 1067 527 4545
Fragments arranged by size:
4545 1067 527 74 73
BamHI G'GATC_C
Cuts at: 3616 3983 3616
Size: 367 5919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2567 2584 3607 5062 618
Size: 325 217 627 780 17 1023 1455 1842
Fragments arranged by size:
1842 1455 1023 780 627 325 217 17
BanII G_rGCy'C
Cuts at: 1391 3117 3605 1391
Size: 1726 488 4072
Fragments arranged by size:
4072 1726 488
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2978 3261 928
Size: 971 1079 283 3953
Fragments arranged by size:
3953 1079 971 283
BbvCI CC'TCA_GC
Cuts at: 3183 3183
Size: 6286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2008 2042 4477 4511 1903
Size: 34 71 34 2435 34 3678
Fragments arranged by size:
3678 2435 71 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3193 4897 136
Size: 107 122 71 631 605 1521 1704 1525
Fragments arranged by size:
1704 1525 1521 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3165 6281 3165
Size: 3116 3170
BlpI GC'TnA_GC
Cuts at: 3575 3703 3575
Size: 128 6158
Bme1580I G_kGCm'C
Cuts at: 1790 2116 4347 5503 1790
Size: 326 2231 1156 2573
Fragments arranged by size:
2573 2231 1156 326
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4017 4975 442
Size: 416 53 83 399 24 2600 958 1753
Fragments arranged by size:
2600 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3412 3412
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4946 1239
Size: 3707 2579
Bpu10I CC'TnA_GC
Cuts at: 3183 3258 3183
Size: 75 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 3201 4411 5276 5574 2174
Size: 1027 1210 865 298 2886
Fragments arranged by size:
2886 1210 1027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3084 4949 882
Size: 617 1585 1865 2219
Fragments arranged by size:
2219 1865 1585 617
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 3302 6280 3302
Size: 2978 3308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2983 4093 4475 5110
Size: 53 83 186 936 1450 1110 382 635
Cuts at: 5110 275
Size: 1451
Fragments arranged by size:
1451 1450 1110 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2286 2316 1178
Size: 30 165 30 883 30 5148
Fragments arranged by size:
5148 883 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6286
BseYI C'CCAG_C
Cuts at: 3009 3191 3570 5489 5985 3009
Size: 182 379 1919 496 3310
Fragments arranged by size:
3310 1919 496 379 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5713
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2929 3117 4347 4432 5503 2929
Size: 188 1230 85 1071 3712
Fragments arranged by size:
3712 1230 1071 188 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2929 3117 3605 4347 4432 5503
Size: 399 326 813 188 488 742 85 1071
Cuts at: 5503 1391
Size: 2174
Fragments arranged by size:
2174 1071 813 742 488 399 326 188
85
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 2277 4175 5905 2277
Size: 1898 1730 2658
Fragments arranged by size:
2658 1898 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3641 844
Size: 2797 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2370 2915 4781 4955 65
Size: 1183 1122 545 1866 174 1396
Fragments arranged by size:
1866 1396 1183 1122 545 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2430 2827 3165 3297 3616 3983 4368 4385
Size: 458 397 338 132 319 367 385 17
Cuts at: 4385 5837 5923 5935 6281 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 458 397 385 367 346 338
319 132 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2608 513
Size: 552 1543 4191
Fragments arranged by size:
4191 1543 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 2720 3802 4596 4616 2720
Size: 1082 794 20 4390
Fragments arranged by size:
4390 1082 794 20
ClaI AT'CG_AT
Cuts at: 1554 3493 1554
Size: 1939 4347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DraIII CAC_nnn'GTG
Cuts at: 2326 2326
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251
Size: 54 1425 50 275 18 1903 889 1629
Cuts at: 6251 8
Size: 43
Fragments arranged by size:
1903 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4216 1353
Size: 2863 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3115 3115
Size: 6286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2610 3255 4349 4946 5733 6001 1239
Size: 393 978 645 1094 597 787 268 1524
Fragments arranged by size:
1524 1094 978 787 645 597 393 268
EcoRI G'AATT_C
Cuts at: 3328 3595 3973 3328
Size: 267 378 5641
Fragments arranged by size:
5641 378 267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2918 2918
Size: 6286
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 2025 5433 1678
Size: 347 3408 2531
Fragments arranged by size:
3408 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3157 3189 4927 4959 5001
Size: 32 135 32 1585 32 1738 32 42
Cuts at: 5001 5033 1373
Size: 32 2626
Fragments arranged by size:
2626 1738 1585 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3427 3630 3876 669
Size: 988 281 1489 203 246 3079
Fragments arranged by size:
3079 1489 988 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 2655 3885 2655
Size: 1230 5056
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2558 3146 5400 5584 1305
Size: 1253 588 2254 184 2007
Fragments arranged by size:
2254 2007 1253 588 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4464
Fragments arranged by size:
4464 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2346 2421 2439 4244 4603 4762 518
Size: 648 1180 75 18 1805 359 159 2042
Fragments arranged by size:
2042 1805 1180 648 359 159 75 18
MspA1I CmG'CkG
Cuts at: 1606 3191 3702 4379 5527 5772 1606
Size: 1585 511 677 1148 245 2120
Fragments arranged by size:
2120 1585 1148 677 511 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5734
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 3644 5189 1132
Size: 97 2415 1545 2229
Fragments arranged by size:
2415 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2286 2318 4355 4387 5868 5900 2286
Size: 32 2037 32 1481 32 2672
Fragments arranged by size:
2672 2037 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PspXI vC'TCGA_Gb
Cuts at: 2017 2017
Size: 6286
PstI C_TGCA'G
Cuts at: 830 2064 2727 3638 830
Size: 1234 663 911 3478
Fragments arranged by size:
3478 1234 911 663
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 3191 3191
Size: 6286
SacI G_AGCT'C
Cuts at: 3117 3117
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 3425 3628 1936
Size: 1489 203 4594
Fragments arranged by size:
4594 1489 203
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 2394 3315 4534 6246 2394
Size: 921 1219 1712 2434
Fragments arranged by size:
2434 1712 1219 921
SexAI A'CCwGG_T
Cuts at: 2968 2968
Size: 6286
SfcI C'TryA_G
Cuts at: 826 1045 2060 2091 2667 2723 3634 4769 5450
Size: 219 1015 31 576 56 911 1135 681
Cuts at: 5450 5641 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1015 911 681 576 219 191
56 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2017 2153 3216 4390 5291 5553
Size: 197 926 74 136 1063 1174 901 262
Cuts at: 5553 820
Size: 1553
Fragments arranged by size:
1553 1174 1063 926 901 262 197 136
74
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 2227 4210 5
Size: 47 2175 1983 2081
Fragments arranged by size:
2175 2081 1983 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 3725 513
Size: 552 2660 3074
Fragments arranged by size:
3074 2660 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3247 4313 4330 4483 4668 5113 325
Size: 1255 1667 1066 17 153 185 445 1498
Fragments arranged by size:
1667 1498 1255 1066 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2392 2482 3313
Size: 274 80 33 51 1443 415 90 831
Cuts at: 3313 4532 6244 96
Size: 1219 1712 138
Fragments arranged by size:
1712 1443 1219 831 415 274 138 90
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 2017 1943
Size: 74 6212
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 2901 4415 2901
Size: 1514 4772
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI