Restriction map for
pFN21AA1097
(Circular) (Six-base) MAPSORT of: pFN21AA1097.seq Check: 5485 from: 1 to: 7438
pFN21AA1097 7438 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3087 3173 4781 1937
Size: 1150 86 1608 4594
Fragments arranged by size:
4594 1608 1150 86
Acc65I G'GTAC_C
Cuts at: 4759 4759
Size: 7438
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2952 3063 3120 3821 4017 4109 4626 4671
Size: 1320 111 57 701 196 92 517 45
Cuts at: 4671 5501 6885 7153 1632
Size: 830 1384 268 1917
Fragments arranged by size:
1917 1384 1320 830 701 517 268 196
111 92 57 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 6337 7314 1203
Size: 5134 977 1327
Fragments arranged by size:
5134 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 3394 4319 6167 3394
Size: 925 1848 4665
Fragments arranged by size:
4665 1848 925
AleI CACnn'nnGTG
Cuts at: 1166 3532 1166
Size: 2366 5072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4481 1184
Size: 3297 4141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4104 4136 4104
Size: 32 7406
AlwNI CAG_nnn'CTG
Cuts at: 2651 3379 4592 5181 6753 2651
Size: 728 1213 589 1572 3336
Fragments arranged by size:
3336 1572 1213 728 589
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 2732 3037 3050 3974 4093 4157 4398 4618
Size: 1239 305 13 924 119 64 241 220
Cuts at: 4618 4747 4973 5125 7234 1493
Size: 129 226 152 2109 1697
Fragments arranged by size:
2109 1697 1239 924 305 241 226 220
152 129 119 64 13
AseI AT'TA_AT
Cuts at: 160 1032 3027 5992 160
Size: 872 1995 2965 1606
Fragments arranged by size:
2965 1995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 3407 4763 1870
Size: 73 1464 1356 4545
Fragments arranged by size:
4545 1464 1356 73
BamHI G'GATC_C
Cuts at: 4768 5135 4768
Size: 367 7071
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3262 4759 6214 618
Size: 325 217 627 1475 1497 1455 1842
Fragments arranged by size:
1842 1497 1475 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4757 1391
Size: 3366 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2844 3456 4257 928
Size: 971 945 612 801 4109
Fragments arranged by size:
4109 971 945 801 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3503 3537 5629 5663 1903
Size: 34 1566 34 2092 34 3678
Fragments arranged by size:
3678 2092 1566 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5330 6540 5330
Size: 1210 6228
BclI T'GATC_A
Cuts at: 1548 3982 1548
Size: 2434 5004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6049 136
Size: 107 122 71 631 605 4377 1525
Fragments arranged by size:
4377 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3421 7433 3421
Size: 4012 3426
BlpI GC'TnA_GC
Cuts at: 3459 4855 3459
Size: 1396 6042
Bme1580I G_kGCm'C
Cuts at: 1790 5499 6655 1790
Size: 3709 1156 2573
Fragments arranged by size:
3709 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2364 5169 6127
Size: 416 53 83 399 24 947 2805 958
Cuts at: 6127 442
Size: 1753
Fragments arranged by size:
2805 1753 958 947 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 4425 4503 6098 1239
Size: 917 2269 78 1595 2579
Fragments arranged by size:
2579 2269 1595 917 78
Bpu10I CC'TnA_GC
Cuts at: 3852 4574 3852
Size: 722 6716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2712 5563 6428 6726 2712
Size: 2851 865 298 3424
Fragments arranged by size:
3424 2851 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3404 6101 882
Size: 617 1905 2697 2219
Fragments arranged by size:
2697 2219 1905 617
BsaAI yAC'GTr
Cuts at: 493 1204 5229 493
Size: 711 4025 2702
Fragments arranged by size:
4025 2702 711
BsaBI GATnn'nnATC
Cuts at: 4512 7432 4512
Size: 2920 4518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5245 5627 6262 275
Size: 53 83 186 936 3712 382 635 1451
Fragments arranged by size:
3712 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4669 5871 6269 6543 6690 7101 4669
Size: 1202 398 274 147 411 5006
Fragments arranged by size:
5006 1202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4104 4134 1178
Size: 30 165 30 2701 30 4482
Fragments arranged by size:
4482 2701 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2382 2837 4093 1186
Size: 1196 455 1256 4531
Fragments arranged by size:
4531 1256 1196 455
BseYI C'CCAG_C
Cuts at: 6641 7137 6641
Size: 496 6942
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3392 1163
Size: 573 1656 5209
Fragments arranged by size:
5209 1656 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5499 5584 6655 5499
Size: 85 1071 6282
Fragments arranged by size:
6282 1071 85
BsmI GAATG_Cn'
Cuts at: 2506 3466 4949 5042 2506
Size: 960 1483 93 4902
Fragments arranged by size:
4902 1483 960 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4757 5499 5584 6655 1391
Size: 399 2967 742 85 1071 2174
Fragments arranged by size:
2967 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 2761 5327 7057 2761
Size: 2566 1730 3142
Fragments arranged by size:
3142 2566 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2596 4290 4793 844
Size: 1752 1694 503 3489
Fragments arranged by size:
3489 1752 1694 503
BsrBI CCG'CTC
Cuts at: 1394 1823 5325 1394
Size: 429 3502 3507
Fragments arranged by size:
3507 3502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2306 5933 6107 65
Size: 1183 1058 3627 174 1396
Fragments arranged by size:
3627 1396 1183 1058 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6082 6269 1700
Size: 110 4272 187 2869
Fragments arranged by size:
4272 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3672 4015 4332 96
Size: 3576 343 317 3202
Fragments arranged by size:
3576 3202 343 317
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 5498 6510 5498
Size: 1012 6426
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7438
BstBI TT'CG_AA
Cuts at: 2017 3161 6242 2017
Size: 1144 3081 3213
Fragments arranged by size:
3213 3081 1144
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7438
BstXI CCAn_nnnn'nTGG
Cuts at: 2124 3534 2124
Size: 1410 6028
BstYI r'GATC_y
Cuts at: 1972 2648 2800 3183 3421 3832 4513 4768 5135
Size: 676 152 383 238 411 681 255 367
Cuts at: 5135 5520 5537 6989 7075 7087 7433 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 681 676 411 385 383 367
346 255 238 152 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6886
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6699
Fragments arranged by size:
6699 683 56
BtsI GCAGTG_nn'
Cuts at: 3380 4475 4954 5748 5768 3380
Size: 1095 479 794 20 5050
Fragments arranged by size:
5050 1095 794 479 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7403
DraI TTT'AAA
Cuts at: 2362 4742 2362
Size: 2380 5058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6445 809
Size: 507 5129 1802
Fragments arranged by size:
5129 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3251 4594 4885
Size: 54 1425 50 275 18 1421 1343 291
Cuts at: 4885 5774 7403 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1421 1343 889 291 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 3557 4046 4056 5368 1353
Size: 891 1313 489 10 1312 3423
Fragments arranged by size:
3423 1313 1312 891 489 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4376 6025 6399 6545 387
Size: 1064 138 2787 1649 374 146 1280
Fragments arranged by size:
2787 1649 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2952 3063 3120 3821 4017 4109
Size: 393 524 796 111 57 701 196 92
Cuts at: 4109 4425 4503 4626 4671 5501 6098 6885 7153
Size: 316 78 123 45 830 597 787 268
Cuts at: 7153 1239
Size: 1524
Fragments arranged by size:
1524 830 796 787 701 597 524 393
316 268 196 123 111 92 78 57
45
EcoNI CCTnn'n_nnAGG
Cuts at: 3237 3237
Size: 7438
EcoO109I rG'GnC_Cy
Cuts at: 4523 4646 4523
Size: 123 7315
EcoRI G'AATT_C
Cuts at: 2732 4747 5125 2732
Size: 2015 378 5045
Fragments arranged by size:
5045 2015 378
EcoRV GAT'ATC
Cuts at: 4089 4089
Size: 7438
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2462 2753 3644 2462
Size: 291 891 6256
Fragments arranged by size:
6256 891 291
FspI TGC'GCA
Cuts at: 5944 5944
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 6585 1678
Size: 4907 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2852 2884 3053 3059 3085
Size: 32 135 32 1280 32 169 6 26
Cuts at: 3085 3091 3380 3412 3868 3875 3900 3907 4255
Size: 6 289 32 456 7 25 7 348
Cuts at: 4255 4287 4305 4337 6079 6111 6153 6185 1373
Size: 32 18 32 1742 32 42 32 2626
Fragments arranged by size:
2626 1742 1280 456 348 289 169 135
42 32 32 32 32 32 32 32
32 26 25 18 7 7 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3088 4324 4782 5028 669
Size: 988 281 1150 1236 458 246 3079
Fragments arranged by size:
3079 1236 1150 988 458 281 246
HindIII A'AGCT_T
Cuts at: 748 4029 748
Size: 3281 4157
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KpnI G_GTAC'C
Cuts at: 4763 4763
Size: 7438
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2720 2832 3506 6552 6736 1305
Size: 1415 112 674 3046 184 2007
Fragments arranged by size:
3046 2007 1415 674 184 112
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3253 4596 10
Size: 54 1425 343 1421 1343 2852
Fragments arranged by size:
2852 1425 1421 1343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3162 3532 4218 4463 5396 5755 5914
Size: 648 1996 370 686 245 933 359 159
Cuts at: 5914 518
Size: 2042
Fragments arranged by size:
2042 1996 933 686 648 370 359 245
159
MspA1I CmG'CkG
Cuts at: 1606 4319 4854 5531 6679 6924 1606
Size: 2713 535 677 1148 245 2120
Fragments arranged by size:
2713 2120 1148 677 535 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6886
NdeI CA'TA_TG
Cuts at: 387 2152 3520 3880 387
Size: 1765 1368 360 3945
Fragments arranged by size:
3945 1765 1368 360
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 2133 4796 6341 1132
Size: 97 904 2663 1545 2229
Fragments arranged by size:
2663 2229 1545 904 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7438
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3440 1108
Size: 2332 5106
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 7438
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4104 4136 5507 5539 7020 7052 4104
Size: 32 1371 32 1481 32 4490
Fragments arranged by size:
4490 1481 1371 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4523 4523
Size: 7438
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PstI C_TGCA'G
Cuts at: 830 4629 4790 830
Size: 3799 161 3478
Fragments arranged by size:
3799 3478 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 4319 4319
Size: 7438
SalI G'TCGA_C
Cuts at: 1936 3086 4780 1936
Size: 1150 1694 4594
Fragments arranged by size:
4594 1694 1150
SapI GCTCTTCn'nnn_
Cuts at: 4046 4046
Size: 7438
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SexAI A'CCwGG_T
Cuts at: 4254 4254
Size: 7438
SfcI C'TryA_G
Cuts at: 826 1045 3489 4045 4625 4786 5921 6602 6793
Size: 219 2444 556 580 161 1135 681 191
Cuts at: 6793 826
Size: 1471
Fragments arranged by size:
2444 1471 1135 681 580 556 219 191
161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 4765 1872
Size: 2893 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2727 3407 5542 6443 6705 820
Size: 197 926 784 680 2135 901 262 1553
Fragments arranged by size:
2135 1553 926 901 784 680 262 197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SspI AAT'ATT
Cuts at: 5 52 2479 5362 5
Size: 47 2427 2883 2081
Fragments arranged by size:
2883 2427 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7438
StyI C'CwwG_G
Cuts at: 513 1065 2083 2110 4877 513
Size: 552 1018 27 2767 3074
Fragments arranged by size:
3074 2767 1018 552 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4719 5465 5482 5635 5820 6265 325
Size: 1255 3139 746 17 153 185 445 1498
Fragments arranged by size:
3139 1498 1255 746 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3672 3724 4015
Size: 274 80 33 51 1443 1695 52 291
Cuts at: 4015 4332 5684 7396 96
Size: 317 1352 1712 138
Fragments arranged by size:
1712 1695 1443 1352 317 291 274 138
80 52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2640 2672 3168 3200 7398 7430 2640
Size: 32 496 32 4198 32 2648
Fragments arranged by size:
4198 2648 496 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7438
XbaI T'CTAG_A
Cuts at: 2918 4774 2918
Size: 1856 5582
XhoI C'TCGA_G
Cuts at: 1943 3407 1943
Size: 1464 5974
XmaI C'CCGG_G
Cuts at: 1870 4763 1870
Size: 2893 4545
XmnI GAAnn'nnTTC
Cuts at: 2736 5567 2736
Size: 2831 4607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI XcmI