Restriction map for
pFN21AA1090
(Circular) (Six-base) MAPSORT of: pFN21AA1090.seq Check: 4910 from: 1 to: 8800
pFN21AA1090 8800 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4130 5125 4130
Size: 995 7805
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6610 278
Size: 53 83 186 936 5074 2468
Fragments arranged by size:
5074 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6143 1937
Size: 4206 4594
Acc65I G'GTAC_C
Cuts at: 6121 6121
Size: 8800
AclI AA'CG_TT
Cuts at: 1563 2667 6927 7300 1563
Size: 1104 4260 373 3063
Fragments arranged by size:
4260 3063 1104 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 3423 3567 3642 3813 3815 3972 4910
Size: 942 849 144 75 171 2 157 938
Cuts at: 4910 4920 5926 6863 8247 8515 1632
Size: 10 1006 937 1384 268 1917
Fragments arranged by size:
1917 1384 1006 942 938 937 849 268
171 157 144 75 10 2
AfeI AGC'GCT
Cuts at: 4190 4190
Size: 8800
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8603
AflIII A'CryG_T
Cuts at: 1203 5862 7699 8676 1203
Size: 4659 1837 977 1327
Fragments arranged by size:
4659 1837 1327 977
AgeI A'CCGG_T
Cuts at: 7631 7631
Size: 8800
AhdI GACnn_n'nnGTC
Cuts at: 5962 7529 5962
Size: 1567 7233
AleI CACnn'nnGTG
Cuts at: 1166 4293 5483 1166
Size: 3127 1190 4483
Fragments arranged by size:
4483 3127 1190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3450 4058 4426 4714 1184
Size: 2266 608 368 288 5270
Fragments arranged by size:
5270 2266 608 368 288
AlwNI CAG_nnn'CTG
Cuts at: 3290 6543 8115 3290
Size: 3253 1572 3975
Fragments arranged by size:
3975 3253 1572
ApaI G_GGCC'C
Cuts at: 2965 2965
Size: 8800
ApaLI G'TGCA_C
Cuts at: 2778 6857 8013 2778
Size: 4079 1156 3565
Fragments arranged by size:
4079 3565 1156
ApoI r'AATT_y
Cuts at: 1493 2009 4214 4754 5264 6109 6335 6487 8596
Size: 516 2205 540 510 845 226 152 2109
Cuts at: 8596 1493
Size: 1697
Fragments arranged by size:
2205 2109 1697 845 540 516 510 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7354 160
Size: 872 6322 1606
Fragments arranged by size:
6322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8800
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 3898 4938 5609 6125 1870
Size: 73 313 1642 1040 671 516 4545
Fragments arranged by size:
4545 1642 1040 671 516 313 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2771 2804 3939 3972 2771
Size: 33 1135 33 7599
Fragments arranged by size:
7599 1135 33 33
BamHI G'GATC_C
Cuts at: 6130 6497 6130
Size: 367 8433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2905 6121 7576 618
Size: 325 217 627 287 831 3216 1455 1842
Fragments arranged by size:
3216 1842 1455 831 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 2965 3912 6119 1391
Size: 1574 947 2207 4072
Fragments arranged by size:
4072 2207 1574 947
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2111 3338 3382 3558 3662 4867 928
Size: 971 212 1227 44 176 104 1205 4861
Fragments arranged by size:
4861 1227 1205 971 212 176 104 44
BbvCI CC'TCA_GC
Cuts at: 2147 3627 2147
Size: 1480 7320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3653 3687 3723 3757 6991 7025 1903
Size: 34 1716 34 36 34 3234 34 3678
Fragments arranged by size:
3678 3234 1716 36 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3591 6692 7902 3591
Size: 3101 1210 4489
Fragments arranged by size:
4489 3101 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8800
BfrBI ATG'CAT
Cuts at: 3782 3782
Size: 8800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2903 3089 3530
Size: 107 122 71 631 605 1231 186 441
Cuts at: 3530 3731 7411 136
Size: 201 3680 1525
Fragments arranged by size:
3680 1525 1231 631 605 441 201 186
122 107 71
BglII A'GATC_T
Cuts at: 2245 2577 5016 8795 2245
Size: 332 2439 3779 2250
Fragments arranged by size:
3779 2439 2250 332
BlpI GC'TnA_GC
Cuts at: 3423 6217 3423
Size: 2794 6006
Bme1580I G_kGCm'C
Cuts at: 1790 2782 2965 6861 8017 1790
Size: 992 183 3896 1156 2573
Fragments arranged by size:
3896 2573 1156 992 183
BmgBI CAC'GTC
Cuts at: 1318 4489 5863 1318
Size: 3171 1374 4255
Fragments arranged by size:
4255 3171 1374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2959 3373 6531
Size: 416 53 83 399 24 1542 414 3158
Cuts at: 6531 7489 442
Size: 958 1753
Fragments arranged by size:
3158 1753 1542 958 416 414 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3288 3754 4153 3288
Size: 466 399 7935
Fragments arranged by size:
7935 466 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2352 2561 3260 7460 1239
Size: 910 203 209 699 4200 2579
Fragments arranged by size:
4200 2579 910 699 209 203
Bpu10I CC'TnA_GC
Cuts at: 2147 3464 3627 2147
Size: 1317 163 7320
Fragments arranged by size:
7320 1317 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2566 3060 3731 5441 6925 7790 8088 2566
Size: 494 671 1710 1484 865 298 3278
Fragments arranged by size:
3278 1710 1484 865 671 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3227 3318 3892 5189 5444 7463 882
Size: 617 1728 91 574 1297 255 2019 2219
Fragments arranged by size:
2219 2019 1728 1297 617 574 255 91
BsaAI yAC'GTr
Cuts at: 493 1204 2794 6591 493
Size: 711 1590 3797 2702
Fragments arranged by size:
3797 2702 1590 711
BsaBI GATnn'nnATC
Cuts at: 5073 8794 5073
Size: 3721 5079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 2811 6607 6989
Size: 53 83 186 936 678 600 3796 382
Cuts at: 6989 7624 275
Size: 635 1451
Fragments arranged by size:
3796 1451 936 678 635 600 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7233 7631 7905 8052 8463 7233
Size: 398 274 147 411 7570
Fragments arranged by size:
7570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3995 4025 5253 5283 1178
Size: 30 165 30 2592 30 1228 30 4695
Fragments arranged by size:
4695 2592 1228 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2530 3032 3261 3306 3318 3461 3730 3999
Size: 1344 502 229 45 12 143 269 269
Cuts at: 3999 5315 5318 1186
Size: 1316 3 4668
Fragments arranged by size:
4668 1344 1316 502 269 269 229 143
45 12 3
BseYI C'CCAG_C
Cuts at: 2157 4552 4625 8003 8499 2157
Size: 2395 73 3378 496 2458
Fragments arranged by size:
3378 2458 2395 496 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3954 4426 4622 5764 5932 1163
Size: 573 2218 472 196 1142 168 4031
Fragments arranged by size:
4031 2218 1142 573 472 196 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6250 7011 8039 1540
Size: 275 186 4249 761 1028 2301
Fragments arranged by size:
4249 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2782 3429 3505 4517 5925 6861 6946 8017 2782
Size: 647 76 1012 1408 936 85 1071 3565
Fragments arranged by size:
3565 1408 1071 1012 936 647 85 76
BsmI GAATG_Cn'
Cuts at: 3186 6311 6404 3186
Size: 3125 93 5582
Fragments arranged by size:
5582 3125 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 8800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2782 2965 3429 3505 3912 4517 5925
Size: 399 992 183 464 76 407 605 1408
Cuts at: 5925 6119 6861 6946 8017 1391
Size: 194 742 85 1071 2174
Fragments arranged by size:
2174 1408 1071 992 742 605 464 407
399 194 183 85 76
BspEI T'CCGG_A
Cuts at: 8463 8463
Size: 8800
BspHI T'CATG_A
Cuts at: 6689 8419 6689
Size: 1730 7070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4130 5125 6155 844
Size: 3286 995 1030 3489
Fragments arranged by size:
3489 3286 1030 995
BsrBI CCG'CTC
Cuts at: 1394 1823 3022 6687 1394
Size: 429 1199 3665 3507
Fragments arranged by size:
3665 3507 1199 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3096 4618 5566 7295 7469 65
Size: 1183 1848 1522 948 1729 174 1396
Fragments arranged by size:
1848 1729 1522 1396 1183 948 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 5577 7444 7631 1700
Size: 110 2877 890 1867 187 2869
Fragments arranged by size:
2877 2869 1867 890 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8800
BssSI C'ACGA_G
Cuts at: 4742 5333 5481 6860 7872 4742
Size: 591 148 1379 1012 5670
Fragments arranged by size:
5670 1379 1012 591 148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2288 4440 4617 5758 5778 794
Size: 1494 2152 177 1141 20 3816
Fragments arranged by size:
3816 2152 1494 1141 177 20
BstBI TT'CG_AA
Cuts at: 7604 7604
Size: 8800
BstEII G'GTnAC_C
Cuts at: 1184 2699 1184
Size: 1515 7285
BstYI r'GATC_y
Cuts at: 1972 2245 2577 2731 5016 6130 6497 6882 6899
Size: 273 332 154 2285 1114 367 385 17
Cuts at: 6899 8351 8437 8449 8795 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2285 1977 1452 1114 385 367 346 332
273 154 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3133 4138 3133
Size: 1005 7795
BtgI C'CryG_G
Cuts at: 513 1065 2004 3726 3816 5145 513
Size: 552 939 1722 90 1329 4168
Fragments arranged by size:
4168 1722 1329 939 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8061
Fragments arranged by size:
8061 683 56
BtsI GCAGTG_nn'
Cuts at: 2098 4443 4455 4886 5605 6316 7110 7130 2098
Size: 2345 12 431 719 711 794 20 3768
Fragments arranged by size:
3768 2345 794 719 711 431 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5581 5616 529
Size: 35 5017 35 3713
Fragments arranged by size:
5017 3713 35 35
DraI TTT'AAA
Cuts at: 6104 6104
Size: 8800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7807 809
Size: 507 6491 1802
Fragments arranged by size:
6491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 3702 4685
Size: 54 1425 50 275 18 373 1499 983
Cuts at: 4685 6247 7136 8765 8
Size: 1562 889 1629 43
Fragments arranged by size:
1629 1562 1499 1425 983 889 373 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6247 1537
Size: 275 4435 4090
Fragments arranged by size:
4435 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2507 2632 2834 3174 3191 3257 3295 3450
Size: 1154 125 202 340 17 66 38 155
Cuts at: 3450 3671 4864 5702 6050 6730 1353
Size: 221 1193 838 348 680 3423
Fragments arranged by size:
3423 1193 1154 838 680 348 340 221
202 155 125 66 38 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3220 6082 6094 7387 7761 7907
Size: 1064 138 1631 2862 12 1293 374 146
Cuts at: 7907 387
Size: 1280
Fragments arranged by size:
2862 1631 1293 1280 1064 374 146 138
12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2352 2561 2574 3260 3423 3567
Size: 393 517 203 209 13 686 163 144
Cuts at: 3567 3642 3813 3815 3972 4910 4920 5926 6863
Size: 75 171 2 157 938 10 1006 937
Cuts at: 6863 7460 8247 8515 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1006 938 937 787 686 597 517
393 268 209 203 171 163 157 144
75 13 10 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 2177
Size: 8800
EcoO109I rG'GnC_Cy
Cuts at: 2548 2961 3604 4055 5230 2548
Size: 413 643 451 1175 6118
Fragments arranged by size:
6118 1175 643 451 413
EcoRI G'AATT_C
Cuts at: 4754 6109 6487 4754
Size: 1355 378 7067
Fragments arranged by size:
7067 1355 378
EcoRV GAT'ATC
Cuts at: 4651 4651
Size: 8800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2582 3055 4657 5842 2434
Size: 148 473 1602 1185 5392
Fragments arranged by size:
5392 1602 1185 473 148
FspI TGC'GCA
Cuts at: 7306 7306
Size: 8800
HaeII r_GCGC'y
Cuts at: 1678 4192 5185 7947 1678
Size: 2514 993 2762 2531
Fragments arranged by size:
2762 2531 2514 993
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2569 2601 3359 3391 3672
Size: 32 135 32 997 32 758 32 281
Cuts at: 3672 3704 3896 3928 5948 5980 7441 7473 7515
Size: 32 192 32 2020 32 1461 32 42
Cuts at: 7515 7547 1373
Size: 32 2626
Fragments arranged by size:
2626 2020 1461 997 758 281 192 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4492 5272 6144 6390 669
Size: 988 281 2554 780 872 246 3079
Fragments arranged by size:
3079 2554 988 872 780 281 246
HindIII A'AGCT_T
Cuts at: 748 4922 748
Size: 4174 4626
HpaI GTT'AAC
Cuts at: 5272 6390 5272
Size: 1118 7682
KpnI G_GTAC'C
Cuts at: 6125 6125
Size: 8800
MfeI C'AATT_G
Cuts at: 5593 6399 5593
Size: 806 7994
MluI A'CGCG_T
Cuts at: 8676 8676
Size: 8800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4338 4452 7914 8098 1305
Size: 3033 114 3462 184 2007
Fragments arranged by size:
3462 3033 2007 184 114
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6978
Fragments arranged by size:
6978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 3011 3191 3408 3432 4293 4586
Size: 648 897 948 180 217 24 861 293
Cuts at: 4586 4658 4988 5483 5856 6758 7117 7276 518
Size: 72 330 495 373 902 359 159 2042
Fragments arranged by size:
2042 948 902 897 861 648 495 373
359 330 293 217 180 159 72 24
MspA1I CmG'CkG
Cuts at: 1606 4018 4278 4293 4365 4386 4556 4602 5027
Size: 2412 260 15 72 21 170 46 425
Cuts at: 5027 5147 5433 5736 5755 5946 6216 6893 8041
Size: 120 286 303 19 191 270 677 1148
Cuts at: 8041 8286 1606
Size: 245 2120
Fragments arranged by size:
2412 2120 1148 677 425 303 286 270
260 245 191 170 120 72 46 21
19 15
NaeI GCC'GGC
Cuts at: 4689 4689
Size: 8800
NcoI C'CATG_G
Cuts at: 513 1065 2004 3726 513
Size: 552 939 1722 5587
Fragments arranged by size:
5587 1722 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8800
NgoMIV G'CCGG_C
Cuts at: 4687 4687
Size: 8800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8800
NotI GC'GGCC_GC
Cuts at: 6247 6247
Size: 8800
NsiI A_TGCA'T
Cuts at: 3784 3784
Size: 8800
NspI r_CATG'y
Cuts at: 1132 1229 3010 3786 5362 6158 7703 1132
Size: 97 1781 776 1576 796 1545 2229
Fragments arranged by size:
2229 1781 1576 1545 796 776 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8800
PciI A'CATG_T
Cuts at: 7699 7699
Size: 8800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2633 3385 1108
Size: 1525 752 6523
Fragments arranged by size:
6523 1525 752
PfoI T'CCnGG_A
Cuts at: 4097 4644 4097
Size: 547 8253
PmeI GTTT'AAAC
Cuts at: 6104 6104
Size: 8800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6869 6901 8382 8414 6869
Size: 32 1481 32 7255
Fragments arranged by size:
7255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3604 4055 3604
Size: 451 8349
PshAI GACnn'nnGTC
Cuts at: 1613 3338 1613
Size: 1725 7075
PsiI TTA'TAA
Cuts at: 6370 6370
Size: 8800
PspOMI G'GGCC_C
Cuts at: 2961 2961
Size: 8800
PstI C_TGCA'G
Cuts at: 830 2178 2731 3106 3232 4127 4622 5518 6152
Size: 1348 553 375 126 895 495 896 634
Cuts at: 6152 830
Size: 3478
Fragments arranged by size:
3478 1348 896 895 634 553 495 375
126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8800
PvuII CAG'CTG
Cuts at: 4018 4293 4602 5433 5755 5946 4018
Size: 275 309 831 322 191 6872
Fragments arranged by size:
6872 831 322 309 275 191
SacII CC_GC'GG
Cuts at: 5148 5148
Size: 8800
SalI G'TCGA_C
Cuts at: 1936 6142 1936
Size: 4206 4594
SapI GCTCTTCn'nnn_
Cuts at: 2834 3450 2834
Size: 616 8184
SbfI CC_TGCA'GG
Cuts at: 2178 5518 6152 2178
Size: 3340 634 4826
Fragments arranged by size:
4826 3340 634
ScaI AGT'ACT
Cuts at: 3268 7048 8760 3268
Size: 3780 1712 3308
Fragments arranged by size:
3780 3308 1712
SfcI C'TryA_G
Cuts at: 826 1045 2174 2727 3102 3228 3609 4123 4618
Size: 219 1129 553 375 126 381 514 495
Cuts at: 4618 4675 5367 5514 6148 7283 7964 8155 826
Size: 57 692 147 634 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 692 681 634 553 514
495 381 375 219 191 147 126 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8800
SmaI CCC'GGG
Cuts at: 1872 2258 3900 6127 1872
Size: 386 1642 2227 4545
Fragments arranged by size:
4545 2227 1642 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2581 3075 3746 5456 6904 7805
Size: 197 926 638 494 671 1710 1448 901
Cuts at: 7805 8067 820
Size: 262 1553
Fragments arranged by size:
1710 1553 1448 926 901 671 638 494
262 197
SnaBI TAC'GTA
Cuts at: 493 6591 493
Size: 6098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8800
SphI G_CATG'C
Cuts at: 1132 6158 1132
Size: 5026 3774
SspI AAT'ATT
Cuts at: 5 52 4726 6724 5
Size: 47 4674 1998 2081
Fragments arranged by size:
4674 2081 1998 47
StuI AGG'CCT
Cuts at: 1517 5789 1517
Size: 4272 4528
StyI C'CwwG_G
Cuts at: 513 1065 2004 2705 3084 3726 4087 4328 5784
Size: 552 939 701 379 642 361 241 1456
Cuts at: 5784 5812 5846 6239 513
Size: 28 34 393 3074
Fragments arranged by size:
3074 1456 939 701 642 552 393 379
361 241 34 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2923 3444 3652 4648 5363 5989 6827
Size: 1255 1343 521 208 996 715 626 838
Cuts at: 6827 6844 6997 7182 7627 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1343 1255 996 838 715 626 521
445 208 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3266 7046 8758
Size: 274 80 33 51 1443 1289 3780 1712
Cuts at: 8758 96
Size: 138
Fragments arranged by size:
3780 1712 1443 1289 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 3995 4027 4401 4433 8760 8792 2067
Size: 32 1896 32 374 32 4327 32 2075
Fragments arranged by size:
4327 2075 1896 374 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8800
XbaI T'CTAG_A
Cuts at: 6136 6136
Size: 8800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2823 3523 2823
Size: 700 8100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8800
XmaI C'CCGG_G
Cuts at: 1870 2256 3898 6125 1870
Size: 386 1642 2227 4545
Fragments arranged by size:
4545 2227 1642 386
XmnI GAAnn'nnTTC
Cuts at: 6929 6929
Size: 8800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6608 276
Size: 53 83 186 936 5074 2468
Fragments arranged by size:
5074 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BsiWI BstXI BstZ17I DraIII
EcoICRI FseI FspAI KasI NarI NruI PacI PmlI
PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI
SrfI SwaI