Restriction map for
pFN21AA1080
(Circular) (Six-base) MAPSORT of: pFN21AA1080.seq Check: 499 from: 1 to: 6544
pFN21AA1080 6544 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2149 2149
Size: 6544
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4354 278
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3887 1937
Size: 1950 4594
Acc65I G'GTAC_C
Cuts at: 3865 3865
Size: 6544
AclI AA'CG_TT
Cuts at: 1563 4671 5044 1563
Size: 3108 373 3063
Fragments arranged by size:
3108 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2395 2489 2609 4607 5991 6259 1632
Size: 763 94 120 1998 1384 268 1917
Fragments arranged by size:
1998 1917 1384 763 268 120 94
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6347
AflIII A'CryG_T
Cuts at: 1203 5443 6420 1203
Size: 4240 977 1327
Fragments arranged by size:
4240 1327 977
AgeI A'CCGG_T
Cuts at: 2637 5375 2637
Size: 2738 3806
AhdI GACnn_n'nnGTC
Cuts at: 5273 5273
Size: 6544
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6544
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6544
AlwNI CAG_nnn'CTG
Cuts at: 2467 3121 3553 3712 4287 5859 2467
Size: 654 432 159 575 1572 3152
Fragments arranged by size:
3152 1572 654 575 432 159
ApaI G_GGCC'C
Cuts at: 2069 2069
Size: 6544
ApaLI G'TGCA_C
Cuts at: 4601 5757 4601
Size: 1156 5388
ApoI r'AATT_y
Cuts at: 1493 2146 2312 2650 2889 3253 3498 3853 4079
Size: 653 166 338 239 364 245 355 226
Cuts at: 4079 4231 6340 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 653 364 355 338 245 239
226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 5098 160
Size: 872 4066 1606
Fragments arranged by size:
4066 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2615 3869 1870
Size: 73 119 553 1254 4545
Fragments arranged by size:
4545 1254 553 119 73
BamHI G'GATC_C
Cuts at: 3874 4241 3874
Size: 367 6177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 3534 3865 5320 618
Size: 325 217 627 1310 437 331 1455 1842
Fragments arranged by size:
1842 1455 1310 627 437 331 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2552 3529 3863 1391
Size: 678 483 977 334 4072
Fragments arranged by size:
4072 977 678 483 334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2442 3044 928
Size: 971 543 602 4428
Fragments arranged by size:
4428 971 602 543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4735 4769 1903
Size: 34 2798 34 3678
Fragments arranged by size:
3678 2798 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4436 5646 4436
Size: 1210 5334
BclI T'GATC_A
Cuts at: 1548 2334 1548
Size: 786 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5155 136
Size: 107 122 71 631 605 337 3146 1525
Fragments arranged by size:
3146 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6539 6539
Size: 6544
BlpI GC'TnA_GC
Cuts at: 2750 3961 2750
Size: 1211 5333
Bme1580I G_kGCm'C
Cuts at: 1790 2069 3293 3537 4605 5761 1790
Size: 279 1224 244 1068 1156 2573
Fragments arranged by size:
2573 1224 1156 1068 279 244
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4275 5233 442
Size: 416 53 83 399 24 2858 958 1753
Fragments arranged by size:
2858 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6544
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3594 3594
Size: 6544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2282 2879 2989 3190 3339 3675 5204
Size: 863 180 597 110 201 149 336 1529
Cuts at: 5204 1239
Size: 2579
Fragments arranged by size:
2579 1529 863 597 336 201 180 149
110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3405 4669 5534 5832 3405
Size: 1264 865 298 4117
Fragments arranged by size:
4117 1264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5207 882
Size: 617 3708 2219
Fragments arranged by size:
3708 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4335 493
Size: 711 3131 2702
Fragments arranged by size:
3131 2702 711
BsaBI GATnn'nnATC
Cuts at: 6538 6538
Size: 6544
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4351 4733 5368 275
Size: 53 83 186 936 2818 382 635 1451
Fragments arranged by size:
2818 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2431 2637 2844 3483 4977 5375 5649 5796 6207
Size: 206 207 639 1494 398 274 147 411
Cuts at: 6207 2431
Size: 2768
Fragments arranged by size:
2768 1494 639 411 398 274 207 206
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 1178
Size: 30 165 30 1269 30 5020
Fragments arranged by size:
5020 1269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2900 3193 3257 1186
Size: 1714 293 64 4473
Fragments arranged by size:
4473 1714 293 64
BseYI C'CCAG_C
Cuts at: 3452 3582 5747 6243 3452
Size: 130 2165 496 3753
Fragments arranged by size:
3753 2165 496 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5971
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 3994 4755 5783 1540
Size: 275 186 15 1978 761 1028 2301
Fragments arranged by size:
2301 1978 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2552 3575 4605 4690 5761 2552
Size: 1023 1030 85 1071 3335
Fragments arranged by size:
3335 1071 1030 1023 85
BsmI GAATG_Cn'
Cuts at: 3671 4055 4148 3671
Size: 384 93 6067
Fragments arranged by size:
6067 384 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2445 2445
Size: 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2552 3293 3529 3537 3575 3863
Size: 399 279 483 741 236 8 38 288
Cuts at: 3863 4605 4690 5761 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 483 399 288 279
236 85 38 8
BspEI T'CCGG_A
Cuts at: 2431 6207 2431
Size: 3776 2768
BspHI T'CATG_A
Cuts at: 4433 6163 4433
Size: 1730 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2149 2791 3050 3085 3899 844
Size: 1305 642 259 35 814 3489
Fragments arranged by size:
3489 1305 814 642 259 35
BsrBI CCG'CTC
Cuts at: 1394 1823 4431 1394
Size: 429 2608 3507
Fragments arranged by size:
3507 2608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5039 5213 65
Size: 1183 3791 174 1396
Fragments arranged by size:
3791 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2637 3646 5188 5375 1700
Size: 110 827 1009 1542 187 2869
Fragments arranged by size:
2869 1542 1009 827 187 110
BsrGI T'GTAC_A
Cuts at: 96 2931 96
Size: 2835 3709
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6544
BssSI C'ACGA_G
Cuts at: 4604 5616 4604
Size: 1012 5532
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2746 794
Size: 1952 4592
BstBI TT'CG_AA
Cuts at: 5348 5348
Size: 6544
BstEII G'GTnAC_C
Cuts at: 1184 2956 1184
Size: 1772 4772
BstXI CCAn_nnnn'nTGG
Cuts at: 2538 2986 2538
Size: 448 6096
BstYI r'GATC_y
Cuts at: 1972 2214 3874 4241 4626 4643 6095 6181 6193
Size: 242 1660 367 385 17 1452 86 12
Cuts at: 6193 6539 1972
Size: 346 1977
Fragments arranged by size:
1977 1660 1452 385 367 346 242 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 513
Size: 552 939 12 5041
Fragments arranged by size:
5041 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5805
Fragments arranged by size:
5805 683 56
BtsI GCAGTG_nn'
Cuts at: 4060 4854 4874 4060
Size: 794 20 5730
Fragments arranged by size:
5730 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3757 3792 529
Size: 35 3193 35 3281
Fragments arranged by size:
3281 3193 35 35
DraI TTT'AAA
Cuts at: 3848 3848
Size: 6544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2999 3827 5551 809
Size: 507 1683 828 1724 1802
Fragments arranged by size:
1802 1724 1683 828 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 3991 4880
Size: 54 1425 50 275 18 477 1684 889
Cuts at: 4880 6509 8
Size: 1629 43
Fragments arranged by size:
1684 1629 1425 889 477 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3991 1537
Size: 275 2179 4090
Fragments arranged by size:
4090 2179 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2386 2414 4474 1353
Size: 1033 28 2060 3423
Fragments arranged by size:
3423 2060 1033 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5131 5505 5651 387
Size: 1064 138 3542 374 146 1280
Fragments arranged by size:
3542 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2550 2550
Size: 6544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2282 2395 2489 2609 2879 2989
Size: 393 470 180 113 94 120 270 110
Cuts at: 2989 3190 3339 3675 4607 5204 5991 6259 1239
Size: 201 149 336 932 597 787 268 1524
Fragments arranged by size:
1524 932 787 597 470 393 336 270
268 201 180 149 120 113 110 94
EcoNI CCTnn'n_nnAGG
Cuts at: 3153 3153
Size: 6544
EcoO109I rG'GnC_Cy
Cuts at: 2057 2364 2620 2776 2794 3118 2057
Size: 307 256 156 18 324 5483
Fragments arranged by size:
5483 324 307 256 156 18
EcoRI G'AATT_C
Cuts at: 3498 3853 4231 3498
Size: 355 378 5811
Fragments arranged by size:
5811 378 355
EcoRV GAT'ATC
Cuts at: 3598 3702 3598
Size: 104 6440
FspI TGC'GCA
Cuts at: 3081 5050 3081
Size: 1969 4575
HaeII r_GCGC'y
Cuts at: 1678 5691 1678
Size: 4013 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2206 2238 2672 2704 3254
Size: 32 135 32 634 32 434 32 550
Cuts at: 3254 3286 5185 5217 5259 5291 1373
Size: 32 1899 32 42 32 2626
Fragments arranged by size:
2626 1899 634 550 434 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3888 4134 669
Size: 988 281 1950 246 3079
Fragments arranged by size:
3079 1950 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2582 3662 748
Size: 1834 1080 3630
Fragments arranged by size:
3630 1834 1080
HpaI GTT'AAC
Cuts at: 4134 4134
Size: 6544
KpnI G_GTAC'C
Cuts at: 3869 3869
Size: 6544
MfeI C'AATT_G
Cuts at: 4143 4143
Size: 6544
MluI A'CGCG_T
Cuts at: 6420 6420
Size: 6544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 2396 5658 5842 1305
Size: 795 296 3262 184 2007
Fragments arranged by size:
3262 2007 795 296 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 10
Size: 54 1425 343 477 4245
Fragments arranged by size:
4245 1425 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4502 4861 5020 518
Size: 648 3336 359 159 2042
Fragments arranged by size:
3336 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 3695 3960 4637 5785 6030 1606
Size: 412 1677 265 677 1148 245 2120
Fragments arranged by size:
2120 1677 1148 677 412 265 245
NaeI GCC'GGC
Cuts at: 3648 3648
Size: 6544
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5053
Fragments arranged by size:
5053 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6544
NgoMIV G'CCGG_C
Cuts at: 3646 3646
Size: 6544
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6544
NotI GC'GGCC_GC
Cuts at: 3991 3991
Size: 6544
NspI r_CATG'y
Cuts at: 1132 1229 2119 2192 2758 3902 5447 1132
Size: 97 890 73 566 1144 1545 2229
Fragments arranged by size:
2229 1545 1144 890 566 97 73
PasI CC'CwG_GG
Cuts at: 1348 2053 3530 1348
Size: 705 1477 4362
Fragments arranged by size:
4362 1477 705
PciI A'CATG_T
Cuts at: 5443 5443
Size: 6544
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3224 1108
Size: 2116 4428
PmeI GTTT'AAAC
Cuts at: 3848 3848
Size: 6544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4613 4645 6126 6158 4613
Size: 32 1481 32 4999
Fragments arranged by size:
4999 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2364 2620 3118 2057
Size: 307 256 498 5483
Fragments arranged by size:
5483 498 307 256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6544
PsiI TTA'TAA
Cuts at: 2486 4114 2486
Size: 1628 4916
PspOMI G'GGCC_C
Cuts at: 2065 2065
Size: 6544
PstI C_TGCA'G
Cuts at: 830 2742 2802 3247 3645 3896 830
Size: 1912 60 445 398 251 3478
Fragments arranged by size:
3478 1912 445 398 251 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6544
PvuII CAG'CTG
Cuts at: 3695 3695
Size: 6544
SacI G_AGCT'C
Cuts at: 2552 2552
Size: 6544
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 6544
SalI G'TCGA_C
Cuts at: 1936 3886 1936
Size: 1950 4594
SanDI GG'GwC_CC
Cuts at: 2057 2057
Size: 6544
SbfI CC_TGCA'GG
Cuts at: 2802 3896 2802
Size: 1094 5450
ScaI AGT'ACT
Cuts at: 4792 6504 4792
Size: 1712 4832
SexAI A'CCwGG_T
Cuts at: 3337 3337
Size: 6544
SfcI C'TryA_G
Cuts at: 826 1045 2738 2798 3243 3641 3892 5027 5708
Size: 219 1693 60 445 398 251 1135 681
Cuts at: 5708 5899 826
Size: 191 1471
Fragments arranged by size:
1693 1471 1135 681 445 398 251 219
191 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6544
SmaI CCC'GGG
Cuts at: 1872 2064 3871 1872
Size: 192 1807 4545
Fragments arranged by size:
4545 1807 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3420 4648 5549 5811 820
Size: 197 926 1477 1228 901 262 1553
Fragments arranged by size:
1553 1477 1228 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4335 493
Size: 3842 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6544
SphI G_CATG'C
Cuts at: 1132 3902 1132
Size: 2770 3774
SspI AAT'ATT
Cuts at: 5 52 4468 5
Size: 47 4416 2081
Fragments arranged by size:
4416 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6544
StyI C'CwwG_G
Cuts at: 513 1065 2004 2917 3129 3324 3772 3983 513
Size: 552 939 913 212 195 448 211 3074
Fragments arranged by size:
3074 939 913 552 448 212 211 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2930 3552 4571 4588 4741 4926 5371
Size: 1255 1350 622 1019 17 153 185 445
Cuts at: 5371 325
Size: 1498
Fragments arranged by size:
1498 1350 1255 1019 622 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2931 4790 6502
Size: 274 80 33 51 1443 954 1859 1712
Cuts at: 6502 96
Size: 138
Fragments arranged by size:
1859 1712 1443 954 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2958 2990 6504 6536 2958
Size: 32 3514 32 2966
Fragments arranged by size:
3514 2966 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6544
XbaI T'CTAG_A
Cuts at: 3880 3880
Size: 6544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2535 3363 2535
Size: 828 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6544
XmaI C'CCGG_G
Cuts at: 1870 2062 3869 1870
Size: 192 1807 4545
Fragments arranged by size:
4545 1807 192
XmnI GAAnn'nnTTC
Cuts at: 3491 3812 4673 3491
Size: 321 861 5362
Fragments arranged by size:
5362 861 321
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4352 276
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SapI SfiI SfoI SrfI SwaI