Restriction map for
pFN21AA1079
(Circular) (Six-base) MAPSORT of: pFN21AA1079.seq Check: 2624 from: 1 to: 9067
pFN21AA1079 9067 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2128 5563 6000 6198 2128
Size: 3435 437 198 4997
Fragments arranged by size:
4997 3435 437 198
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6877 278
Size: 53 83 186 936 5341 2468
Fragments arranged by size:
5341 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3264 6096 6410 1937
Size: 1327 2832 314 4594
Fragments arranged by size:
4594 2832 1327 314
Acc65I G'GTAC_C
Cuts at: 4447 4489 6388 4447
Size: 42 1899 7126
Fragments arranged by size:
7126 1899 42
AclI AA'CG_TT
Cuts at: 1563 7194 7567 1563
Size: 5631 373 3063
Fragments arranged by size:
5631 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2582 2690 3225 3311 4223 4290 4301
Size: 932 18 108 535 86 912 67 11
Cuts at: 4301 7130 8514 8782 1632
Size: 2829 1384 268 1917
Fragments arranged by size:
2829 1917 1384 932 912 535 268 108
86 67 18 11
AfeI AGC'GCT
Cuts at: 3714 5028 3714
Size: 1314 7753
AflII C'TTAA_G
Cuts at: 820 1017 4250 820
Size: 197 3233 5637
Fragments arranged by size:
5637 3233 197
AflIII A'CryG_T
Cuts at: 1203 3871 4532 7966 8943 1203
Size: 2668 661 3434 977 1327
Fragments arranged by size:
3434 2668 1327 977 661
AgeI A'CCGG_T
Cuts at: 7898 7898
Size: 9067
AhdI GACnn_n'nnGTC
Cuts at: 4951 7796 4951
Size: 2845 6222
AleI CACnn'nnGTG
Cuts at: 1166 5261 1166
Size: 4095 4972
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2118 4439 5457 1184
Size: 934 2321 1018 4794
Fragments arranged by size:
4794 2321 1018 934
AlwNI CAG_nnn'CTG
Cuts at: 3418 3554 4589 4973 5607 6810 8382 3418
Size: 136 1035 384 634 1203 1572 4103
Fragments arranged by size:
4103 1572 1203 1035 634 384 136
ApaI G_GGCC'C
Cuts at: 3561 5765 6138 3561
Size: 2204 373 6490
Fragments arranged by size:
6490 2204 373
ApaLI G'TGCA_C
Cuts at: 3212 7124 8280 3212
Size: 3912 1156 3999
Fragments arranged by size:
3999 3912 1156
ApoI r'AATT_y
Cuts at: 1493 2237 4608 6376 6602 6754 8863 1493
Size: 744 2371 1768 226 152 2109 1697
Fragments arranged by size:
2371 2109 1768 1697 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2177 7621 160
Size: 872 1145 5444 1606
Fragments arranged by size:
5444 1606 1145 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9067
AvaI C'yCGr_G
Cuts at: 1870 1943 3887 5123 5147 5342 5365 5414 5429
Size: 73 1944 1236 24 195 23 49 15
Cuts at: 5429 5810 6041 6392 1870
Size: 381 231 351 4545
Fragments arranged by size:
4545 1944 1236 381 351 231 195 73
49 24 23 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4374 4407 4374
Size: 33 9034
BamHI G'GATC_C
Cuts at: 6397 6764 6397
Size: 367 8700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 4447 4489 5153 5988
Size: 325 217 627 326 2334 42 664 835
Cuts at: 5988 6388 7843 618
Size: 400 1455 1842
Fragments arranged by size:
2334 1842 1455 835 664 627 400 326
325 217 42
BanII G_rGCy'C
Cuts at: 1391 3561 3896 5343 5765 6138 6386 1391
Size: 2170 335 1447 422 373 248 4072
Fragments arranged by size:
4072 2170 1447 422 373 335 248
BbeI G_GCGC'C
Cuts at: 2117 2117
Size: 9067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 2311 4422 4806 5595 928
Size: 971 393 19 2111 384 789 4400
Fragments arranged by size:
4400 2111 971 789 393 384 19
BbvCI CC'TCA_GC
Cuts at: 5921 5969 5921
Size: 48 9019
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3335 3369 5693 5727 7258
Size: 34 50 34 1314 34 2324 34 1531
Cuts at: 7258 7292 1903
Size: 34 3678
Fragments arranged by size:
3678 2324 1531 1314 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2212 6959 8169 2212
Size: 4747 1210 3110
Fragments arranged by size:
4747 3110 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7678 136
Size: 107 122 71 631 605 6006 1525
Fragments arranged by size:
6006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9062 9062
Size: 9067
BlpI GC'TnA_GC
Cuts at: 3447 6314 6484 3447
Size: 2867 170 6030
Fragments arranged by size:
6030 2867 170
Bme1580I G_kGCm'C
Cuts at: 1790 2483 3216 3561 5073 5156 5765 5961 6138
Size: 693 733 345 1512 83 609 196 177
Cuts at: 6138 7128 8284 1790
Size: 990 1156 2573
Fragments arranged by size:
2573 1512 1156 990 733 693 609 345
196 177 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3936 4775 6798
Size: 416 53 83 399 24 2519 839 2023
Cuts at: 6798 7756 442
Size: 958 1753
Fragments arranged by size:
2519 2023 1753 958 839 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2919 3149 3627 4961 5024 5500 5629 7727
Size: 1680 230 478 1334 63 476 129 2098
Cuts at: 7727 1239
Size: 2579
Fragments arranged by size:
2579 2098 1680 1334 478 476 230 129
63
Bpu10I CC'TnA_GC
Cuts at: 3419 3779 5921 5969 6167 6203 3419
Size: 360 2142 48 198 36 6283
Fragments arranged by size:
6283 2142 360 198 48 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2379 3815 7192 8057 8355 2379
Size: 1436 3377 865 298 3091
Fragments arranged by size:
3377 3091 1436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3009 3187 4673 5936 6107 7730 882
Size: 617 1510 178 1486 1263 171 1623 2219
Fragments arranged by size:
2219 1623 1510 1486 1263 617 178 171
BsaAI yAC'GTr
Cuts at: 493 1204 3874 4533 6858 493
Size: 711 2670 659 2325 2702
Fragments arranged by size:
2702 2670 2325 711 659
BsaBI GATnn'nnATC
Cuts at: 9061 9061
Size: 9067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 6874 7256 7891
Size: 53 83 186 936 581 4760 382 635
Cuts at: 7891 275
Size: 1451
Fragments arranged by size:
4760 1451 936 635 581 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3520 7500 7898 8172 8319 8730 3520
Size: 3980 398 274 147 411 3857
Fragments arranged by size:
3980 3857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8842
Fragments arranged by size:
8842 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3307 3812 4691 4900 5372 5876 6159 1186
Size: 2121 505 879 209 472 504 283 4094
Fragments arranged by size:
4094 2121 879 505 504 472 283 209
BseYI C'CCAG_C
Cuts at: 2075 2308 2350 3123 3367 3765 4524 4966 5568
Size: 233 42 773 244 398 759 442 602
Cuts at: 5568 6130 6175 6181 8270 8766 2075
Size: 562 45 6 2089 496 2376
Fragments arranged by size:
2376 2089 773 759 602 562 496 442
398 244 233 45 42 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 2182 2765 2774 4463 5656 1163
Size: 573 388 58 583 9 1689 1193 4574
Fragments arranged by size:
4574 1689 1193 583 573 388 58 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5665 6517 7278 8306 1540
Size: 275 186 3664 852 761 1028 2301
Fragments arranged by size:
3664 2301 1028 852 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2700 3216 4418 5952 7128 7213 8284 2700
Size: 516 1202 1534 1176 85 1071 3483
Fragments arranged by size:
3483 1534 1202 1176 1071 516 85
BsmI GAATG_Cn'
Cuts at: 3332 4261 5626 6578 6671 3332
Size: 929 1365 952 93 5728
Fragments arranged by size:
5728 1365 952 929 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5267 5267
Size: 9067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2483 2700 3216 3561 3896 4418 5073
Size: 399 693 217 516 345 335 522 655
Cuts at: 5073 5156 5343 5765 5952 5961 6138 6386 7128
Size: 83 187 422 187 9 177 248 742
Cuts at: 7128 7213 8284 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 693 655 522 516 422
399 345 335 248 217 187 187 177
85 83 9
BspEI T'CCGG_A
Cuts at: 8730 8730
Size: 9067
BspHI T'CATG_A
Cuts at: 6956 8686 6956
Size: 1730 7337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2128 2862 3240 4009 4435 5563 5904 5919
Size: 1284 734 378 769 426 1128 341 15
Cuts at: 5919 6000 6198 6318 6422 844
Size: 81 198 120 104 3489
Fragments arranged by size:
3489 1284 1128 769 734 426 378 341
198 120 104 81 15
BsrBI CCG'CTC
Cuts at: 1394 1823 5144 6171 6954 1394
Size: 429 3321 1027 783 3507
Fragments arranged by size:
3507 3321 1027 783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 6341 7562 7736 65
Size: 1183 3173 1920 1221 174 1396
Fragments arranged by size:
3173 1920 1396 1221 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2066 3363 4818 5083 5237 7711
Size: 110 207 49 1297 1455 265 154 2474
Cuts at: 7711 7898 1700
Size: 187 2869
Fragments arranged by size:
2869 2474 1455 1297 265 207 187 154
110 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9067
BssSI C'ACGA_G
Cuts at: 3505 7127 8139 3505
Size: 3622 1012 4433
Fragments arranged by size:
4433 3622 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2779 2788 3220 5956 794
Size: 1985 9 432 2736 3905
Fragments arranged by size:
3905 2736 1985 432 9
BstBI TT'CG_AA
Cuts at: 4464 7871 4464
Size: 3407 5660
BstEII G'GTnAC_C
Cuts at: 1184 2663 1184
Size: 1479 7588
BstXI CCAn_nnnn'nTGG
Cuts at: 5524 6130 5524
Size: 606 8461
BstYI r'GATC_y
Cuts at: 1972 3550 5045 6280 6397 6764 7149 7166 8618
Size: 1578 1495 1235 117 367 385 17 1452
Cuts at: 8618 8704 8716 9062 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1578 1495 1452 1235 385 367 346
117 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3596 6187 3596
Size: 2591 6476
BtgI C'CryG_G
Cuts at: 513 1065 2754 3198 5178 5583 5685 513
Size: 552 1689 444 1980 405 102 3895
Fragments arranged by size:
3895 1980 1689 552 444 405 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2938 4799 5199 5256 5655 487
Size: 683 56 1712 1861 400 57 399 3899
Fragments arranged by size:
3899 1861 1712 683 400 399 57 56
BtsI GCAGTG_nn'
Cuts at: 2077 3284 6035 6583 7377 7397 2077
Size: 1207 2751 548 794 20 3747
Fragments arranged by size:
3747 2751 1207 794 548 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9032
DraI TTT'AAA
Cuts at: 3997 4854 6371 3997
Size: 857 1517 6693
Fragments arranged by size:
6693 1517 857
DraIII CAC_nnn'GTG
Cuts at: 5956 5956
Size: 9067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3260 8074 809
Size: 507 1944 4814 1802
Fragments arranged by size:
4814 1944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 3580 5191
Size: 54 1425 50 275 18 933 817 1611
Cuts at: 5191 6018 6514 7403 9032 8
Size: 827 496 889 1629 43
Fragments arranged by size:
1629 1611 1425 933 889 827 817 496
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6514 1537
Size: 275 4702 4090
Fragments arranged by size:
4702 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6997 1353
Size: 5644 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 5371 7654 8028 8174 387
Size: 1064 138 455 3327 2283 374 146 1280
Fragments arranged by size:
3327 2283 1280 1064 455 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2582 2690 2919 3149 3225 3311
Size: 393 932 18 108 229 230 76 86
Cuts at: 3311 3627 4223 4290 4301 4961 5024 5500 5629
Size: 316 596 67 11 660 63 476 129
Cuts at: 5629 7130 7727 8514 8782 1239
Size: 1501 597 787 268 1524
Fragments arranged by size:
1524 1501 932 787 660 597 596 476
393 316 268 230 229 129 108 86
76 67 63 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 4940 5837 4940
Size: 897 8170
EcoO109I rG'GnC_Cy
Cuts at: 2215 3415 3601 4650 5362 5761 5893 6134 6135
Size: 1200 186 1049 712 399 132 241 1
Cuts at: 6135 2215
Size: 5147
Fragments arranged by size:
5147 1200 1049 712 399 241 186 132
1
EcoRI G'AATT_C
Cuts at: 6376 6754 6376
Size: 378 8689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2800 2476
Size: 324 8743
FspI TGC'GCA
Cuts at: 2684 7573 2684
Size: 4889 4178
HaeII r_GCGC'y
Cuts at: 1678 2117 3716 3779 5030 8214 1678
Size: 439 1599 63 1251 3184 2531
Fragments arranged by size:
3184 2531 1599 1251 439 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 4634 4666 4840
Size: 32 135 32 707 32 2323 32 174
Cuts at: 4840 4872 4937 4969 5122 5154 5318 5336 5350
Size: 32 65 32 153 32 164 18 14
Cuts at: 5350 5368 6257 6289 7708 7740 7782 7814 1373
Size: 18 889 32 1419 32 42 32 2626
Fragments arranged by size:
2626 2323 1419 889 707 174 164 153
135 65 42 32 32 32 32 32
32 32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3265 6339 6411 6657 669
Size: 988 281 1327 3074 72 246 3079
Fragments arranged by size:
3079 3074 1327 988 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9067
HpaI GTT'AAC
Cuts at: 6657 6657
Size: 9067
KasI G'GCGC_C
Cuts at: 2113 2113
Size: 9067
KpnI G_GTAC'C
Cuts at: 4451 4493 6392 4451
Size: 42 1899 7126
Fragments arranged by size:
7126 1899 42
MfeI C'AATT_G
Cuts at: 6666 6666
Size: 9067
MluI A'CGCG_T
Cuts at: 8943 8943
Size: 9067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2587 2853 3090 3345 3625 5913 8181 8365
Size: 1282 266 237 255 280 2288 2268 184
Cuts at: 8365 1305
Size: 2007
Fragments arranged by size:
2288 2268 2007 1282 280 266 255 237
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7245
Fragments arranged by size:
7245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4230 5261 7025 7384 7543 518
Size: 648 3064 1031 1764 359 159 2042
Fragments arranged by size:
3064 2042 1764 1031 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2756 3127 3200 3250 3579 5199 5285 5637
Size: 1150 371 73 50 329 1620 86 352
Cuts at: 5637 6017 6483 7160 8308 8553 1606
Size: 380 466 677 1148 245 2120
Fragments arranged by size:
2120 1620 1150 1148 677 466 380 371
352 329 245 86 73 50
NaeI GCC'GGC
Cuts at: 2019 2068 4820 5085 5239 2019
Size: 49 2752 265 154 5847
Fragments arranged by size:
5847 2752 265 154 49
NarI GG'CG_CC
Cuts at: 2114 2114
Size: 9067
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8515
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9067
NgoMIV G'CCGG_C
Cuts at: 2017 2066 4818 5083 5237 2017
Size: 49 2752 265 154 5847
Fragments arranged by size:
5847 2752 265 154 49
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9067
NotI GC'GGCC_GC
Cuts at: 6514 6514
Size: 9067
NspI r_CATG'y
Cuts at: 1132 1229 2704 4345 6425 7970 1132
Size: 97 1475 1641 2080 1545 2229
Fragments arranged by size:
2229 2080 1641 1545 1475 97
PasI CC'CwG_GG
Cuts at: 1348 5293 1348
Size: 3945 5122
PciI A'CATG_T
Cuts at: 7966 7966
Size: 9067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4639 1108
Size: 3531 5536
PfoI T'CCnGG_A
Cuts at: 4551 4758 4938 4551
Size: 207 180 8680
Fragments arranged by size:
8680 207 180
PmeI GTTT'AAAC
Cuts at: 6371 6371
Size: 9067
PmlI CAC'GTG
Cuts at: 3874 4533 3874
Size: 659 8408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7136 7168 8649 8681 7136
Size: 32 1481 32 7522
Fragments arranged by size:
7522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 3601 4650 5362 5893 2215
Size: 1386 1049 712 531 5389
Fragments arranged by size:
5389 1386 1049 712 531
PshAI GACnn'nnGTC
Cuts at: 1613 4578 1613
Size: 2965 6102
PsiI TTA'TAA
Cuts at: 6637 6637
Size: 9067
PspOMI G'GGCC_C
Cuts at: 3557 5761 6134 3557
Size: 2204 373 6490
Fragments arranged by size:
6490 2204 373
PstI C_TGCA'G
Cuts at: 830 2128 2733 2977 3243 6419 830
Size: 1298 605 244 266 3176 3478
Fragments arranged by size:
3478 3176 1298 605 266 244
PvuI CG_AT'CG
Cuts at: 2001 5665 2001
Size: 3664 5403
PvuII CAG'CTG
Cuts at: 3127 5285 3127
Size: 2158 6909
SacII CC_GC'GG
Cuts at: 2757 3201 2757
Size: 444 8623
SalI G'TCGA_C
Cuts at: 1936 3263 6409 1936
Size: 1327 3146 4594
Fragments arranged by size:
4594 3146 1327
SanDI GG'GwC_CC
Cuts at: 3601 3601
Size: 9067
SbfI CC_TGCA'GG
Cuts at: 6419 6419
Size: 9067
ScaI AGT'ACT
Cuts at: 7315 9027 7315
Size: 1712 7355
SfcI C'TryA_G
Cuts at: 826 1045 2124 2376 2729 2973 3239 6415 7550
Size: 219 1079 252 353 244 266 3176 1135
Cuts at: 7550 8231 8422 826
Size: 681 191 1471
Fragments arranged by size:
3176 1471 1135 1079 681 353 266 252
244 219 191
SfoI GGC'GCC
Cuts at: 2115 2115
Size: 9067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9067
SgrAI Cr'CCGG_yG
Cuts at: 1700 2017 1700
Size: 317 8750
SmaI CCC'GGG
Cuts at: 1872 5367 6394 1872
Size: 3495 1027 4545
Fragments arranged by size:
4545 3495 1027
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2394 3794 4250 7171 8072 8334
Size: 197 926 451 1400 456 2921 901 262
Cuts at: 8334 820
Size: 1553
Fragments arranged by size:
2921 1553 1400 926 901 456 451 262
197
SnaBI TAC'GTA
Cuts at: 493 6858 493
Size: 6365 2702
SpeI A'CTAG_T
Cuts at: 152 5065 152
Size: 4913 4154
SphI G_CATG'C
Cuts at: 1132 6425 1132
Size: 5293 3774
SspI AAT'ATT
Cuts at: 5 52 2175 2341 2593 3957 6991 5
Size: 47 2123 166 252 1364 3034 2081
Fragments arranged by size:
3034 2123 2081 1364 252 166 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9067
StyI C'CwwG_G
Cuts at: 513 1065 5385 6506 513
Size: 552 4320 1121 3074
Fragments arranged by size:
4320 3074 1121 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 2999 3416 5960 7094 7111 7264
Size: 1255 1071 348 417 2544 1134 17 153
Cuts at: 7264 7449 7894 325
Size: 185 445 1498
Fragments arranged by size:
2544 1498 1255 1134 1071 445 417 348
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 3833 7313
Size: 274 80 33 51 1443 1829 27 3480
Cuts at: 7313 9025 96
Size: 1712 138
Fragments arranged by size:
3480 1829 1712 1443 274 138 80 51
33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9027 9059 9027
Size: 32 9035
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9067
XbaI T'CTAG_A
Cuts at: 6403 6403
Size: 9067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3364 4661 5728 3364
Size: 1297 1067 6703
Fragments arranged by size:
6703 1297 1067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9067
XmaI C'CCGG_G
Cuts at: 1870 5365 6392 1870
Size: 3495 1027 4545
Fragments arranged by size:
4545 3495 1027
XmnI GAAnn'nnTTC
Cuts at: 2625 7196 2625
Size: 4571 4496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6875 276
Size: 53 83 186 936 5341 2468
Fragments arranged by size:
5341 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacI SapI SexAI SfiI SrfI SwaI