Restriction map for
pFN21AA1071
(Circular) (Six-base) MAPSORT of: pFN21AA1071.seq Check: 1515 from: 1 to: 7957
pFN21AA1071 7957 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3057 3057
Size: 7957
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5767 278
Size: 53 83 186 936 4231 2468
Fragments arranged by size:
4231 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5300 1937
Size: 3363 4594
Acc65I G'GTAC_C
Cuts at: 5278 5278
Size: 7957
AclI AA'CG_TT
Cuts at: 1563 3957 6084 6457 1563
Size: 2394 2127 373 3063
Fragments arranged by size:
3063 2394 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 2714 5004 6020 7404 7672 1632
Size: 405 677 2290 1016 1384 268 1917
Fragments arranged by size:
2290 1917 1384 1016 677 405 268
AflII C'TTAA_G
Cuts at: 820 1017 2501 820
Size: 197 1484 6276
Fragments arranged by size:
6276 1484 197
AflIII A'CryG_T
Cuts at: 1203 6856 7833 1203
Size: 5653 977 1327
Fragments arranged by size:
5653 1327 977
AgeI A'CCGG_T
Cuts at: 5102 6788 5102
Size: 1686 6271
AhdI GACnn_n'nnGTC
Cuts at: 6686 6686
Size: 7957
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7957
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3642 1184
Size: 2458 5499
AlwNI CAG_nnn'CTG
Cuts at: 3739 4667 4745 4772 4796 4832 4853 4874 5215
Size: 928 78 27 24 36 21 21 341
Cuts at: 5215 5700 7272 3739
Size: 485 1572 4424
Fragments arranged by size:
4424 1572 928 485 341 78 36 27
24 21 21
ApaI G_GGCC'C
Cuts at: 2174 2174
Size: 7957
ApaLI G'TGCA_C
Cuts at: 6014 7170 6014
Size: 1156 6801
ApoI r'AATT_y
Cuts at: 1493 2011 2651 5266 5492 5644 7753 1493
Size: 518 640 2615 226 152 2109 1697
Fragments arranged by size:
2615 2109 1697 640 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6511 160
Size: 872 5479 1606
Fragments arranged by size:
5479 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7957
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 3237 5282 1870
Size: 73 224 1070 2045 4545
Fragments arranged by size:
4545 2045 1070 224 73
AvrII C'CTAG_G
Cuts at: 3053 4474 3053
Size: 1421 6536
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4576 4609 4576
Size: 33 7924
BamHI G'GATC_C
Cuts at: 5287 5654 5287
Size: 367 7590
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5011 5278 6733 618
Size: 325 217 627 3224 267 1455 1842
Fragments arranged by size:
3224 1842 1455 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2174 3244 3307 3993 5276 1391
Size: 783 1070 63 686 1283 4072
Fragments arranged by size:
4072 1283 1070 783 686 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6986
BbvCI CC'TCA_GC
Cuts at: 4867 5079 4867
Size: 212 7745
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4534 4568 6148 6182 1903
Size: 34 2597 34 1580 34 3678
Fragments arranged by size:
3678 2597 1580 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5849 7059 5849
Size: 1210 6747
BclI T'GATC_A
Cuts at: 1548 4287 1548
Size: 2739 5218
BfrBI ATG'CAT
Cuts at: 3517 3517
Size: 7957
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2697 6568 136
Size: 107 122 71 631 605 1025 3871 1525
Fragments arranged by size:
3871 1525 1025 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7952 7952
Size: 7957
BlpI GC'TnA_GC
Cuts at: 3243 5374 3243
Size: 2131 5826
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2787 6018 7174 1790
Size: 384 613 3231 1156 2573
Fragments arranged by size:
3231 2573 1156 613 384
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7957
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 2347 5688
Size: 416 53 83 399 24 728 202 3341
Cuts at: 5688 6646 442
Size: 958 1753
Fragments arranged by size:
3341 1753 958 728 416 399 202 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7957
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3343 3343
Size: 7957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2427 3827 3917 4283 4644 4722 4749 4773
Size: 1188 1400 90 366 361 78 27 24
Cuts at: 4773 4809 4905 4920 4956 5028 5046 6617 1239
Size: 36 96 15 36 72 18 1571 2579
Fragments arranged by size:
2579 1571 1400 1188 366 361 96 90
78 72 36 36 27 24 18 15
Bpu10I CC'TnA_GC
Cuts at: 4867 4983 5073 5079 4867
Size: 116 90 6 7745
Fragments arranged by size:
7745 116 90 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2545 3367 6082 6947 7245 2545
Size: 822 2715 865 298 3257
Fragments arranged by size:
3257 2715 865 822 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2490 3027 6620 882
Size: 617 991 537 3593 2219
Fragments arranged by size:
3593 2219 991 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 5748 493
Size: 711 4544 2702
Fragments arranged by size:
4544 2702 711
BsaBI GATnn'nnATC
Cuts at: 7951 7951
Size: 7957
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2440 5764 6146 6781
Size: 53 83 186 936 907 3324 382 635
Cuts at: 6781 275
Size: 1451
Fragments arranged by size:
3324 1451 936 907 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3339 3885 4960 5102 6390 6788 7062 7209 7620
Size: 546 1075 142 1288 398 274 147 411
Cuts at: 7620 3339
Size: 3676
Fragments arranged by size:
3676 1288 1075 546 411 398 274 147
142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2578 2608 3095 3125 5008
Size: 30 165 30 1175 30 487 30 1883
Cuts at: 5008 5032 5038 5044 5062 5074 1178
Size: 24 6 6 18 12 4061
Fragments arranged by size:
4061 1883 1175 487 165 30 30 30
30 24 18 12 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2286 4022 4079 4208 1186
Size: 1100 1736 57 129 4935
Fragments arranged by size:
4935 1736 1100 129 57
BseYI C'CCAG_C
Cuts at: 3306 7160 7656 3306
Size: 3854 496 3607
Fragments arranged by size:
3854 3607 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3963 1163
Size: 573 2227 5157
Fragments arranged by size:
5157 2227 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5407 6168 7196 1540
Size: 275 186 3406 761 1028 2301
Fragments arranged by size:
3406 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2596 3879 3993 6018 6103 7174 2596
Size: 1283 114 2025 85 1071 3379
Fragments arranged by size:
3379 2025 1283 1071 114 85
BsmI GAATG_Cn'
Cuts at: 2307 4479 5468 5561 2307
Size: 2172 989 93 4703
Fragments arranged by size:
4703 2172 989 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3113 3424 3887 4443 4791 3113
Size: 311 463 556 348 6279
Fragments arranged by size:
6279 556 463 348 311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2596 2787 3244 3307 3879 3993
Size: 399 384 422 191 457 63 572 114
Cuts at: 3993 5276 6018 6103 7174 1391
Size: 1283 742 85 1071 2174
Fragments arranged by size:
2174 1283 1071 742 572 457 422 399
384 191 114 85 63
BspEI T'CCGG_A
Cuts at: 3885 7620 3885
Size: 3735 4222
BspHI T'CATG_A
Cuts at: 5846 7576 5846
Size: 1730 6227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3057 5150 5312 844
Size: 2213 2093 162 3489
Fragments arranged by size:
3489 2213 2093 162
BsrBI CCG'CTC
Cuts at: 1394 1823 5844 1394
Size: 429 4021 3507
Fragments arranged by size:
4021 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2894 6452 6626 65
Size: 1183 1646 3558 174 1396
Fragments arranged by size:
3558 1646 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5102 6601 6788 1700
Size: 110 3292 1499 187 2869
Fragments arranged by size:
3292 2869 1499 187 110
BsrGI T'GTAC_A
Cuts at: 96 3835 96
Size: 3739 4218
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7957
BssSI C'ACGA_G
Cuts at: 3440 6017 7029 3440
Size: 2577 1012 4368
Fragments arranged by size:
4368 2577 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2056 4637 794
Size: 1262 2581 4114
Fragments arranged by size:
4114 2581 1262
BstBI TT'CG_AA
Cuts at: 6761 6761
Size: 7957
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7957
BstXI CCAn_nnnn'nTGG
Cuts at: 4723 5188 4723
Size: 465 7492
BstYI r'GATC_y
Cuts at: 1972 3321 3532 5211 5287 5654 6039 6056 7508
Size: 1349 211 1679 76 367 385 17 1452
Cuts at: 7508 7594 7606 7952 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1679 1452 1349 385 367 346 211
86 76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2040 4722 513
Size: 552 975 2682 3748
Fragments arranged by size:
3748 2682 975 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2786 487
Size: 683 56 1560 5658
Fragments arranged by size:
5658 1560 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 3681 4677 4692 4698 4794 5473 6267 6287
Size: 1517 996 15 6 96 679 794 20
Cuts at: 6287 2164
Size: 3834
Fragments arranged by size:
3834 1517 996 794 679 96 20 15
6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7957
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5103 5138 529
Size: 35 4539 35 3348
Fragments arranged by size:
4539 3348 35 35
DraI TTT'AAA
Cuts at: 5261 5261
Size: 7957
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4130 6964 809
Size: 507 2814 2834 1802
Fragments arranged by size:
2834 2814 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2553 3678 4212
Size: 54 1425 50 275 18 723 1125 534
Cuts at: 4212 4352 5404 6293 7922 8
Size: 140 1052 889 1629 43
Fragments arranged by size:
1629 1425 1125 1052 889 723 534 275
140 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5404 1537
Size: 275 3592 4090
Fragments arranged by size:
4090 3592 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3771 5887 1353
Size: 2418 2116 3423
Fragments arranged by size:
3423 2418 2116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6544 6918 7064 387
Size: 1064 138 4955 374 146 1280
Fragments arranged by size:
4955 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3991 3991
Size: 7957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2427 2714 3827 3917 4283 4644
Size: 393 405 390 287 1113 90 366 361
Cuts at: 4644 4722 4749 4773 4809 4905 4920 4956 5004
Size: 78 27 24 36 96 15 36 48
Cuts at: 5004 5028 5046 6020 6617 7404 7672 1239
Size: 24 18 974 597 787 268 1524
Fragments arranged by size:
1524 1113 974 787 597 405 393 390
366 361 287 268 96 90 78 48
36 36 27 24 24 18 15
EcoO109I rG'GnC_Cy
Cuts at: 2171 2714 2958 3466 3736 3829 2171
Size: 543 244 508 270 93 6299
Fragments arranged by size:
6299 543 508 270 244 93
EcoRI G'AATT_C
Cuts at: 2651 5266 5644 2651
Size: 2615 378 4964
Fragments arranged by size:
4964 2615 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2087 2690 2087
Size: 603 7354
FspI TGC'GCA
Cuts at: 6463 6463
Size: 7957
HaeII r_GCGC'y
Cuts at: 1678 7104 1678
Size: 5426 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2830 2862 3529 3561 3702
Size: 32 135 32 1258 32 667 32 141
Cuts at: 3702 3734 4006 4038 4325 4357 5131 5163 6598
Size: 32 272 32 287 32 774 32 1435
Cuts at: 6598 6630 6672 6704 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1435 1258 774 667 287 272 141
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3805 5301 5547 669
Size: 988 281 1867 1496 246 3079
Fragments arranged by size:
3079 1867 1496 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7957
HpaI GTT'AAC
Cuts at: 5547 5547
Size: 7957
KpnI G_GTAC'C
Cuts at: 5282 5282
Size: 7957
MfeI C'AATT_G
Cuts at: 5556 5556
Size: 7957
MluI A'CGCG_T
Cuts at: 7833 7833
Size: 7957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2365 3339 5063 5075 5081 7071 7255 1305
Size: 1060 974 1724 12 6 1990 184 2007
Fragments arranged by size:
2007 1990 1724 1060 974 184 12 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 3680 4214 10
Size: 54 1425 343 723 1125 534 3753
Fragments arranged by size:
3753 1425 1125 723 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 2481 4721 5915 6274 6433 518
Size: 648 1213 102 2240 1194 359 159 2042
Fragments arranged by size:
2240 2042 1213 1194 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2290 2455 4339 4634 4769 4802 4829 4850
Size: 684 165 1884 295 135 33 27 21
Cuts at: 4850 4871 4925 4979 5066 5373 6050 7198 7443
Size: 21 54 54 87 307 677 1148 245
Cuts at: 7443 1606
Size: 2120
Fragments arranged by size:
2120 1884 1148 684 677 307 295 245
165 135 87 54 54 33 27 21
21
NcoI C'CATG_G
Cuts at: 513 1065 4722 513
Size: 552 3657 3748
Fragments arranged by size:
3748 3657 552
NdeI CA'TA_TG
Cuts at: 387 3430 387
Size: 3043 4914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7957
NotI GC'GGCC_GC
Cuts at: 5404 5404
Size: 7957
NsiI A_TGCA'T
Cuts at: 3519 3519
Size: 7957
NspI r_CATG'y
Cuts at: 1132 1229 2378 2516 2746 3855 4351 5315 6860
Size: 97 1149 138 230 1109 496 964 1545
Cuts at: 6860 1132
Size: 2229
Fragments arranged by size:
2229 1545 1149 1109 964 496 230 138
97
PasI CC'CwG_GG
Cuts at: 1348 2245 1348
Size: 897 7060
PciI A'CATG_T
Cuts at: 6856 6856
Size: 7957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3313 1108
Size: 2205 5752
PfoI T'CCnGG_A
Cuts at: 2464 4310 5107 2464
Size: 1846 797 5314
Fragments arranged by size:
5314 1846 797
PmeI GTTT'AAAC
Cuts at: 5261 5261
Size: 7957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6026 6058 7539 7571 6026
Size: 32 1481 32 6412
Fragments arranged by size:
6412 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2958 2958
Size: 7957
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7957
PsiI TTA'TAA
Cuts at: 5527 5527
Size: 7957
PspOMI G'GGCC_C
Cuts at: 2170 2170
Size: 7957
PstI C_TGCA'G
Cuts at: 830 3393 4592 4633 5309 830
Size: 2563 1199 41 676 3478
Fragments arranged by size:
3478 2563 1199 676 41
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7957
PvuII CAG'CTG
Cuts at: 2290 4339 4634 4769 4829 4850 4871 4925 5066
Size: 2049 295 135 60 21 21 54 141
Cuts at: 5066 2290
Size: 5181
Fragments arranged by size:
5181 2049 295 141 135 60 54 21
21
SacI G_AGCT'C
Cuts at: 3993 3993
Size: 7957
SalI G'TCGA_C
Cuts at: 1936 5299 1936
Size: 3363 4594
SapI GCTCTTCn'nnn_
Cuts at: 3771 3771
Size: 7957
SbfI CC_TGCA'GG
Cuts at: 5309 5309
Size: 7957
ScaI AGT'ACT
Cuts at: 2357 4296 6205 7917 2357
Size: 1939 1909 1712 2397
Fragments arranged by size:
2397 1939 1909 1712
SfcI C'TryA_G
Cuts at: 826 1045 2757 2791 3389 4588 4629 5305 6440
Size: 219 1712 34 598 1199 41 676 1135
Cuts at: 6440 7121 7312 826
Size: 681 191 1471
Fragments arranged by size:
1712 1471 1199 1135 681 676 598 219
191 41 34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7957
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7957
SmaI CCC'GGG
Cuts at: 1872 2169 5284 1872
Size: 297 3115 4545
Fragments arranged by size:
4545 3115 297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 2524 3346 6061 6962 7224
Size: 197 926 558 23 822 2715 901 262
Cuts at: 7224 820
Size: 1553
Fragments arranged by size:
2715 1553 926 901 822 558 262 197
23
SnaBI TAC'GTA
Cuts at: 493 5748 493
Size: 5255 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7957
SphI G_CATG'C
Cuts at: 1132 2378 2746 4351 5315 1132
Size: 1246 368 1605 964 3774
Fragments arranged by size:
3774 1605 1246 964 368
SspI AAT'ATT
Cuts at: 5 52 4058 5881 5
Size: 47 4006 1823 2081
Fragments arranged by size:
4006 2081 1823 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7957
StyI C'CwwG_G
Cuts at: 513 1065 2340 2665 3015 3053 3756 4474 4722
Size: 552 1275 325 350 38 703 718 248
Cuts at: 4722 5396 513
Size: 674 3074
Fragments arranged by size:
3074 1275 718 703 674 552 350 325
248 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4478 5984 6001 6154 6339 6784 325
Size: 1255 2898 1506 17 153 185 445 1498
Fragments arranged by size:
2898 1506 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 3835 4294
Size: 274 80 33 51 1443 378 1480 459
Cuts at: 4294 6203 7915 96
Size: 1909 1712 138
Fragments arranged by size:
1909 1712 1480 1443 459 378 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7917 7949 7917
Size: 32 7925
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7957
XbaI T'CTAG_A
Cuts at: 3547 4171 5293 3547
Size: 624 1122 6211
Fragments arranged by size:
6211 1122 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4604 5212 4604
Size: 608 7349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7957
XmaI C'CCGG_G
Cuts at: 1870 2167 5282 1870
Size: 297 3115 4545
Fragments arranged by size:
4545 3115 297
XmnI GAAnn'nnTTC
Cuts at: 6086 6086
Size: 7957
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5765 276
Size: 53 83 186 936 4231 2468
Fragments arranged by size:
4231 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BsiWI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI