Restriction map for
pFN21AA1065
(Circular) (Six-base) MAPSORT of: pFN21AA1065.seq Check: 3469 from: 1 to: 6628
pFN21AA1065 6628 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3877 3971 1937
Size: 1940 94 4594
Fragments arranged by size:
4594 1940 94
Acc65I G'GTAC_C
Cuts at: 3152 3949 3152
Size: 797 5831
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2822 3114 3328 4691 6075 6343 1632
Size: 710 480 292 214 1363 1384 268 1917
Fragments arranged by size:
1917 1384 1363 710 480 292 268 214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 2275 3285 5527 6504 1203
Size: 1072 1010 2242 977 1327
Fragments arranged by size:
2242 1327 1072 1010 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 5357 5357
Size: 6628
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2387 1184
Size: 1203 5425
AlwNI CAG_nnn'CTG
Cuts at: 2320 2671 3688 4371 5943 2320
Size: 351 1017 683 1572 3005
Fragments arranged by size:
3005 1572 1017 683 351
ApaI G_GGCC'C
Cuts at: 2108 2440 2641 3041 3071 3548 3845 2108
Size: 332 201 400 30 477 297 4891
Fragments arranged by size:
4891 477 400 332 297 201 30
ApaLI G'TGCA_C
Cuts at: 4685 5841 4685
Size: 1156 5472
ApoI r'AATT_y
Cuts at: 1493 2958 3383 3937 4163 4315 6424 1493
Size: 1465 425 554 226 152 2109 1697
Fragments arranged by size:
2109 1697 1465 554 425 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 2766 3034 3953 1870
Size: 73 823 268 919 4545
Fragments arranged by size:
4545 919 823 268 73
BamHI G'GATC_C
Cuts at: 3958 4325 3958
Size: 367 6261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3152 3187 3625 3949 5404
Size: 325 217 627 1365 35 438 324 1455
Cuts at: 5404 618
Size: 1842
Fragments arranged by size:
1842 1455 1365 627 438 325 324 217
35
BanII G_rGCy'C
Cuts at: 1391 2108 2440 2579 2641 3041 3063 3071 3342
Size: 717 332 139 62 400 22 8 271
Cuts at: 3342 3548 3716 3845 3947 1391
Size: 206 168 129 102 4072
Fragments arranged by size:
4072 717 400 332 271 206 168 139
129 102 62 22 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4819 4853 1903
Size: 34 2882 34 3678
Fragments arranged by size:
3678 2882 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4520 5730 4520
Size: 1210 5418
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6628
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2724 3647 5239
Size: 107 122 71 631 605 1052 923 1592
Cuts at: 5239 136
Size: 1525
Fragments arranged by size:
1592 1525 1052 923 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6623 6623
Size: 6628
BlpI GC'TnA_GC
Cuts at: 2877 4045 2877
Size: 1168 5460
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2440 2621 2641 3041 3071 3192 3520
Size: 318 332 181 20 400 30 121 328
Cuts at: 3520 3548 3845 4689 5845 1790
Size: 28 297 844 1156 2573
Fragments arranged by size:
2573 1156 844 400 332 328 318 297
181 121 30 28 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6628
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3598 3710 3848
Size: 416 53 83 399 24 2181 112 138
Cuts at: 3848 4359 5317 442
Size: 511 958 1753
Fragments arranged by size:
2181 1753 958 511 416 399 138 112
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2503 2819 3216 3244 5288 1239
Size: 1264 316 397 28 2044 2579
Fragments arranged by size:
2579 2044 1264 397 316 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2390 3484 4753 5618 5916 2390
Size: 1094 1269 865 298 3102
Fragments arranged by size:
3102 1269 1094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5291 882
Size: 617 3792 2219
Fragments arranged by size:
3792 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4419 493
Size: 711 3215 2702
Fragments arranged by size:
3215 2702 711
BsaBI GATnn'nnATC
Cuts at: 6622 6622
Size: 6628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4435 4817 5452 275
Size: 53 83 186 936 2902 382 635 1451
Fragments arranged by size:
2902 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 5233
Fragments arranged by size:
5233 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2672 2846 1186
Size: 1207 279 174 4968
Fragments arranged by size:
4968 1207 279 174
BseYI C'CCAG_C
Cuts at: 2283 2622 2644 2736 3029 3266 3509 3681 5831
Size: 339 22 92 293 237 243 172 2150
Cuts at: 5831 6327 2283
Size: 496 2584
Fragments arranged by size:
2584 2150 496 339 293 243 237 172
92 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6055
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4078 4839 5867 1540
Size: 275 186 2077 761 1028 2301
Fragments arranged by size:
2301 2077 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3342 3637 4689 4774 5845 3342
Size: 295 1052 85 1071 4125
Fragments arranged by size:
4125 1071 1052 295 85
BsmI GAATG_Cn'
Cuts at: 4139 4232 4139
Size: 93 6535
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2440 2579 2621 2641 3041 3063
Size: 399 318 332 139 42 20 400 22
Cuts at: 3063 3071 3192 3342 3520 3548 3637 3716 3845
Size: 8 121 150 178 28 89 79 129
Cuts at: 3845 3947 4689 4774 5845 1391
Size: 102 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 332 318 178
150 139 129 121 102 89 85 79
42 28 22 20 8
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 2172 4517 6247 2172
Size: 2345 1730 2553
Fragments arranged by size:
2553 2345 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3983 844
Size: 3139 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4515 1394
Size: 429 2692 3507
Fragments arranged by size:
3507 2692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3855 5123 5297 65
Size: 1183 2607 1268 174 1396
Fragments arranged by size:
2607 1396 1268 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5272 5459 1700
Size: 110 3462 187 2869
Fragments arranged by size:
3462 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2226 96
Size: 2130 4498
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 4688 5700 4688
Size: 1012 5616
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3632 794
Size: 2838 3790
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 3580 1184
Size: 2396 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 2485 2822 3449 3772 3902 2485
Size: 337 627 323 130 5211
Fragments arranged by size:
5211 627 337 323 130
BstYI r'GATC_y
Cuts at: 1972 3958 4325 4710 4727 6179 6265 6277 6623
Size: 1986 367 385 17 1452 86 12 346
Cuts at: 6623 1972
Size: 1977
Fragments arranged by size:
1986 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3878 3878
Size: 6628
Bsu36I CC'TnA_GG
Cuts at: 2604 2894 2604
Size: 290 6338
BtgI C'CryG_G
Cuts at: 513 1065 2099 2203 2593 3042 3179 3765 3789
Size: 552 1034 104 390 449 137 586 24
Cuts at: 3789 513
Size: 3352
Fragments arranged by size:
3352 1034 586 552 449 390 137 104
24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5889
Fragments arranged by size:
5889 683 56
BtsI GCAGTG_nn'
Cuts at: 2294 3520 3874 4144 4938 4958 2294
Size: 1226 354 270 794 20 3964
Fragments arranged by size:
3964 1226 794 354 270 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6593
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DraIII CAC_nnn'GTG
Cuts at: 3632 3632
Size: 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 5635 809
Size: 507 698 3621 1802
Fragments arranged by size:
3621 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3140 4075 4964
Size: 54 1425 50 275 18 1310 935 889
Cuts at: 4964 6593 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1310 935 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4075 1537
Size: 275 2263 4090
Fragments arranged by size:
4090 2263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2823 3347 4558 1353
Size: 1470 524 1211 3423
Fragments arranged by size:
3423 1470 1211 524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5215 5589 5735 387
Size: 1064 138 3626 374 146 1280
Fragments arranged by size:
3626 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3340 3340
Size: 6628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2503 2819 2822 3114 3216 3244
Size: 393 710 161 316 3 292 102 28
Cuts at: 3244 3328 4691 5288 6075 6343 1239
Size: 84 1363 597 787 268 1524
Fragments arranged by size:
1524 1363 787 710 597 393 316 292
268 161 102 84 28 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3750 3750
Size: 6628
EcoO109I rG'GnC_Cy
Cuts at: 2104 2436 2637 2638 3038 3067 3330 3545 3842
Size: 332 201 1 400 29 263 215 297
Cuts at: 3842 2104
Size: 4890
Fragments arranged by size:
4890 400 332 297 263 215 201 29
1
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2852 2852
Size: 6628
FspI TGC'GCA
Cuts at: 5134 5134
Size: 6628
HaeII r_GCGC'y
Cuts at: 1678 2237 2683 5775 1678
Size: 559 446 3092 2531
Fragments arranged by size:
3092 2531 559 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5269 5301 5343 5375 1373
Size: 32 135 32 3697 32 42 32 2626
Fragments arranged by size:
3697 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3658 3972 4218 669
Size: 988 281 1720 314 246 3079
Fragments arranged by size:
3079 1720 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6628
HpaI GTT'AAC
Cuts at: 3658 4218 3658
Size: 560 6068
KpnI G_GTAC'C
Cuts at: 3156 3953 3156
Size: 797 5831
MfeI C'AATT_G
Cuts at: 4227 4227
Size: 6628
MluI A'CGCG_T
Cuts at: 3285 6504 3285
Size: 3219 3409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2988 5742 5926 1305
Size: 1683 2754 184 2007
Fragments arranged by size:
2754 2007 1683 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3142 10
Size: 54 1425 343 1310 3496
Fragments arranged by size:
3496 1425 1310 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2369 2483 3770 4586 4945 5104
Size: 648 1011 192 114 1287 816 359 159
Cuts at: 5104 518
Size: 2042
Fragments arranged by size:
2042 1287 1011 816 648 359 192 159
114
MspA1I CmG'CkG
Cuts at: 1606 2644 2842 2848 3044 3113 3164 3688 3791
Size: 1038 198 6 196 69 51 524 103
Cuts at: 3791 4044 4721 5869 6114 1606
Size: 253 677 1148 245 2120
Fragments arranged by size:
2120 1148 1038 677 524 253 245 198
196 103 69 51 6
NcoI C'CATG_G
Cuts at: 513 1065 2203 2593 3179 3765 513
Size: 552 1138 390 586 586 3376
Fragments arranged by size:
3376 1138 586 586 552 390
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6628
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 2225 3986 5531 1132
Size: 97 996 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 996 97
PasI CC'CwG_GG
Cuts at: 1348 3063 3099 3234 1348
Size: 1715 36 135 4742
Fragments arranged by size:
4742 1715 135 36
PciI A'CATG_T
Cuts at: 5527 5527
Size: 6628
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3099 3149 1108
Size: 1991 50 4587
Fragments arranged by size:
4587 1991 50
PfoI T'CCnGG_A
Cuts at: 2076 2076
Size: 6628
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2071 3557 3589 4697 4729 6210 6242 2039
Size: 32 1486 32 1108 32 1481 32 2425
Fragments arranged by size:
2425 1486 1481 1108 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3330 3330
Size: 6628
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6628
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PspOMI G'GGCC_C
Cuts at: 2104 2436 2637 3037 3067 3544 3841 2104
Size: 332 201 400 30 477 297 4891
Fragments arranged by size:
4891 477 400 332 297 201 30
PstI C_TGCA'G
Cuts at: 830 2667 2847 3980 830
Size: 1837 180 1133 3478
Fragments arranged by size:
3478 1837 1133 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
PvuII CAG'CTG
Cuts at: 2842 2848 3164 2842
Size: 6 316 6306
Fragments arranged by size:
6306 316 6
SacI G_AGCT'C
Cuts at: 3342 3342
Size: 6628
SacII CC_GC'GG
Cuts at: 3045 3792 3045
Size: 747 5881
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SapI GCTCTTCn'nnn_
Cuts at: 3347 3347
Size: 6628
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 3127 4876 6588 3127
Size: 1749 1712 3167
Fragments arranged by size:
3167 1749 1712
SexAI A'CCwGG_T
Cuts at: 3628 3628
Size: 6628
SfcI C'TryA_G
Cuts at: 826 1045 2663 2843 3976 5111 5792 5983 826
Size: 219 1618 180 1133 1135 681 191 1471
Fragments arranged by size:
1618 1471 1135 1133 681 219 191 180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 3955 1872
Size: 2083 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 3499 4732 5633 5895 820
Size: 197 926 462 1094 1233 901 262 1553
Fragments arranged by size:
1553 1233 1094 926 901 462 262 197
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 2819 152
Size: 2667 3961
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SspI AAT'ATT
Cuts at: 5 52 4552 5
Size: 47 4500 2081
Fragments arranged by size:
4500 2081 47
StuI AGG'CCT
Cuts at: 1517 2609 3589 1517
Size: 1092 980 4556
Fragments arranged by size:
4556 1092 980
StyI C'CwwG_G
Cuts at: 513 1065 2203 2593 3179 3765 4067 513
Size: 552 1138 390 586 586 302 3074
Fragments arranged by size:
3074 1138 586 586 552 390 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2141 2358 4655 4672 4825 5010 5455
Size: 1255 561 217 2297 17 153 185 445
Cuts at: 5455 325
Size: 1498
Fragments arranged by size:
2297 1498 1255 561 445 217 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2334 3125
Size: 274 80 33 51 1443 249 108 791
Cuts at: 3125 4874 6586 96
Size: 1749 1712 138
Fragments arranged by size:
1749 1712 1443 791 274 249 138 108
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3198 3230 6588 6620 3198
Size: 32 3358 32 3206
Fragments arranged by size:
3358 3206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2014 1316
Size: 698 5930
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 2477 2504 2727 3556 2461
Size: 16 27 223 829 5533
Fragments arranged by size:
5533 829 223 27 16
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 3953 1870
Size: 2083 4545
XmnI GAAnn'nnTTC
Cuts at: 2303 2327 4757 2303
Size: 24 2430 4174
Fragments arranged by size:
4174 2430 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SanDI SfiI SfoI SrfI SwaI